Дослідження впливу вуглецевих нанотрубок на протопласти тютюну для створення нових підходів у біотехнології рослин
Aims. In order to develop the background for the creation of novel carbon nanotubes (CNTs) based approaches in plant biotechnology the effect of non-covalently functionalized single-walled and multi-walled CNTs (SWCNTs and MWCNTs, respectively) on tobacco Nicotiana tabacum L. mesophyll protoplasts...
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| Published in: | Фактори експериментальної еволюції організмів |
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| Date: | 2015 |
| Main Authors: | , , , |
| Format: | Article |
| Language: | Ukrainian |
| Published: |
Інститут молекулярної біології і генетики НАН України
2015
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| Subjects: | |
| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/177495 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Дослідження впливу вуглецевих нанотрубок на протопласти тютюну для створення нових підходів у біотехнології рослин / О.М. Бурлака, Я.В. Пірко, А.І. Ємець, Я.Б. Блюм // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 17. — С. 121-125. — Бібліогр.: 19 назв. — укр. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Summary: | Aims. In order to develop the background for the creation of novel carbon nanotubes (CNTs) based approaches in plant
biotechnology the effect of non-covalently functionalized single-walled and multi-walled CNTs (SWCNTs and MWCNTs,
respectively) on tobacco Nicotiana tabacum L. mesophyll protoplasts was investigated. Factors influencing the viability of
protoplasts exposed to CNTs, namely dose, CNTs type, functionalization type, were determined. Methods. Physical and
chemical CNTs surface modification methods, in vitro plant culture approaches and microscope studies were combined to
evaluate the effect of CNTs on protoplasts. Results. The dose-dependent adverse impact of CNTs on viability of protoplasts
was observed. However protoplasts demonstrated higher viability when exposed to SWCNTs in compere to MWCNTs.
Absence of change in protoplasts viability was observed when protoplasts were exposed to identical concentrations of CNTs
functionalized using different molecules, indicating equal biological compatibility of all three types of used functionalization
coatings.
Keywords: carbon nanotubes – CNTs, functionalization, protoplasts, plant biotechnology.
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| ISSN: | 2219-3782 |