Детекція гена Or5 стійкості до вовчка соняшнику за допомогою SCAR-маркеру RTS05
Aims. The SCAR-marker RTS05 are linked to Or5-gene mapped on 5.6 сМ from it. The aim of this work was investigation of the presence of SCAR-marker RTS05 on various breeding initial material of sunflower. Methods. We studied the presence of RTS05 marker in 37 inbred lines, 29 interspecific hybrids-...
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| Veröffentlicht in: | Фактори експериментальної еволюції організмів |
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| Datum: | 2015 |
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | Ukrainian |
| Veröffentlicht: |
Інститут молекулярної біології і генетики НАН України
2015
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| Schlagworte: | |
| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/177496 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Детекція гена Or5 стійкості до вовчка соняшнику за допомогою SCAR-маркеру RTS05 / В.М. Попов, Ю.М. Тереняк, Г.Є. Акініна, Я.Ю. Шарипіна, Т.А. Долгова, В.В. Кириченко // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 17. — С. 330-332. — Бібліогр.: 10 назв. — укр. |
Institution
Digital Library of Periodicals of National Academy of Sciences of Ukraine| Zusammenfassung: | Aims. The SCAR-marker RTS05 are linked to Or5-gene mapped on 5.6 сМ from it. The aim of this work was investigation
of the presence of SCAR-marker RTS05 on various breeding initial material of sunflower. Methods. We studied the
presence of RTS05 marker in 37 inbred lines, 29 interspecific hybrids-based samples and 39 varieties of sunflower that
created in The Plant Production Institute nd. a. V. Ya. Yuriev. The identification of RTS05 marker we conducted by
polymerase chain reaction (PCR). The amplicon of 650 bp size as a result of amplification was indicated about the
presence of Or5-gene. In the case of absence Or5-gene in sunflower samples this specific amplicon was not synthesized.
Results. The amplicon of 650 bp size was identified in all type of studied sunflower breeding material. The frequency of
RTS05-marker in sunflower samples was different. Conclusions The results of our work are suggested possibility to use
sunflower samples with identified Or5-gene for creation initial breeding material resistant to broomrape.
Keywords: sunflower, broomrape, DNA-markers, Or5-gene.
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| ISSN: | 2219-3782 |