Реконструкція та верифікація просторової структури молекул тубулінів представників Diplomonadida, Kinetoplastida, Amoebida, Saccharomycetes та Microsporea
Aims. The purpose of this study was a reconstruction and verification of spatial structures of tubulin molecules from
 representatives of Diplomonadida, Kinetoplastida, Amoebida, Saccharomycetes and Microsporea to determine differences
 in their spatial structures and clarify the mec...
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| Published in: | Фактори експериментальної еволюції організмів |
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| Date: | 2015 |
| Main Authors: | , , , , |
| Format: | Article |
| Language: | Ukrainian |
| Published: |
Інститут молекулярної біології і генетики НАН України
2015
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| Subjects: | |
| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/177499 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Реконструкція та верифікація просторової структури молекул тубулінів представників Diplomonadida, Kinetoplastida, Amoebida, Saccharomycetes та Microsporea / С.І. Співак, С.П. Ожерєдов, Д.О. Самофалова, О.В. Раєвський, П.А. Карпов // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 17. — С. 92-96. — Бібліогр.: 10 назв. — укр. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Summary: | Aims. The purpose of this study was a reconstruction and verification of spatial structures of tubulin molecules from
representatives of Diplomonadida, Kinetoplastida, Amoebida, Saccharomycetes and Microsporea to determine differences
in their spatial structures and clarify the mechanism of their interaction with antimitotic agents. Methods. The study was
carried out with in silico approaches using UniProtКВ database, RCSB Protein Data Bank and on-line services: SWISSMODEL, I-TASSER and MolProbity. Results. 17 tubulin molecules with fully decoded primary structures were selected
among representatives of such classes: Diplomonadida, Kinetoplastida, Amoebida, Saccharomycetes and Microsporea.
For all these proteins accurate three dimentional structures were constructed with subsequent optimization and verification
of protein geometry. Conclusions. Selected models of tubulin molecules were added to a CSModDB database within VO
CSLabGrid for their future application in comparative analysis and a search of compounds with anti mitotic activity by
means of high-throughput screening.
Keywords: tubulin, antimitotic drugs, in silico.
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| ISSN: | 2219-3782 |