Вплив манітолу на індукцію та регенерацію морфогенної калусної тканини у кукурудзи

Aims. In maize (Zea mays L.) callusogenesis on immature embryos is the basis for obtaining somaclones, cell selection,
 genetic engineering. The aim of the study was to examine the effect of mannitol in an induction medium on obtaining and
 regeneration capacity of morphogenic calli....

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Published in:Фактори експериментальної еволюції організмів
Date:2015
Main Authors: Абраімова, О.Є., Деркач, К.В., Сатарова, Т.М., Шевченко, Н.В., Черноусова, Н.М.
Format: Article
Language:Ukrainian
Published: Інститут молекулярної біології і генетики НАН України 2015
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Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/177503
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Вплив манітолу на індукцію та регенерацію морфогенної калусної тканини у кукурудзи/ О.Є. Абраімова, К.В. Деркач, Т.М. Сатарова, Н.В. Шевченко, Н.М. Черноусова // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 17. — С. 103-106. — Бібліогр.: 11 назв. — укр.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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Summary:Aims. In maize (Zea mays L.) callusogenesis on immature embryos is the basis for obtaining somaclones, cell selection,
 genetic engineering. The aim of the study was to examine the effect of mannitol in an induction medium on obtaining and
 regeneration capacity of morphogenic calli. Methods. Plant tissue culture method for assessing morphogenetic reaction of
 9 maize lines was used. Results. Mannitol significantly increased frequencies of morphogenic calli induction, reduced their
 differentiation, but inhibited fresh weight accumulation. Calli obtained on the mannitol contained medium had improved
 regeneration ability. Conclusions. Mannitol is an effective component for the induction of morphogenic calli of different
 types and for maintenance of regeneration ability of callus tissue of type I.
 Keywords: mannitol, maize, culture in vitro, morphogenic callus, regenerants.
ISSN:2219-3782