Застосування наноматеріалу в ембріогенетичній системі in vitro отримання ембріонів свиней

• Опубліковано в: Фактори експериментальної еволюції організмів
• Дата: 2015
• ISSN: 2219-3782
• Автори: Зюзюн, А.Б., Щербак, О.В., Осипчук, О.С., Ковтун, С.І., Дзіцюк, В.В.
• Формат: Стаття
• Мова: Українська
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2015
• Теми: Молекулярні та клітинні біотехнології
• Онлайн доступ: https://nasplib.isofts.kiev.ua/handle/123456789/177518
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Застосування наноматеріалу в ембріогенетичній системі in vitro отримання ембріонів свиней / А.Б. Зюзюн, О.В. Щербак, О.С. Осипчук, С.І. Ковтун, В.В. Дзіцюк // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 17. — С. 164-168. — Бібліогр.: 20 назв. — укр.

Aims. The optimization model culturing somatic and germ cells of animals through the use of nanomaterials currently is
 an important task in the system of development and improvement of effective ways to get in vitro oocytes preimplantant
 embryos and embryonic stem cells. Methods. To research the oocyte-cumulus complexes (OCC), pigs were divided into
 two groups: the experimental group, in which cultivation was carried out in a medium containing 0.001 % concentration
 of UFS / N-Gal and the control group, in which cultivation OCC conducted without the addition of nanomaterials.
 Ejaculated cryopreserved sperm of boar was used for in vitro fertilization of pig’s oocytes. Results. Morphological and
 cytogenetic analysis showed that the level of maturation of oocytes in vitro in the experimental group was 13.6 % higher
 compared to the control group (70.6 % ± 3.6). To investigate the in vitro maturation of oocytes pigs spent their fertilization
 of cryopreserved boar sperm. The quantity of zygotes in the experimental group was formed outside the body by 11.0 %
 more than in the control group (31.8 % ± 4.9), also level splitting of embryos was higher at 15.1 % in the experimental
 group compared with controls (18.2 % ± 4.1). Conclusion. The system of optimization in conditions of in vitro biological
 activity of nanomaterials was developed and the methods of biotechnological manipulation of gametes females in the
 system of rational use and reproduction of native breeds of farm animals were improved.
 Keywords: oocytes, in vitro fertilization, embryos, nanomaterial, N-galactosamine, cytogenetic analysis.