Вивчення ролі NO та актинових філаментів у відповіді рослин на дію холоду
Aims. It has been shown in a number of studies that low temperature leads to significant changes in the organization
 of cytoskeleton structures. Recent studies have shed light on the importance of NO, a ubiquitous signaling molecule
 in eukaryotes, for plant tolerance to freezing. M...
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| Published in: | Фактори експериментальної еволюції організмів |
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| Date: | 2015 |
| Main Authors: | , , , |
| Format: | Article |
| Language: | Ukrainian |
| Published: |
Інститут молекулярної біології і генетики НАН України
2015
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| Subjects: | |
| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/177519 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Вивчення ролі NO та актинових філаментів у відповіді рослин на дію холоду / С.Г. Плоховська, Ю.А. Красиленко, А.І. Ємець, Я.Б. Блюм // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 17. — С. 236-240. — Бібліогр.: 15 назв. — укр. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Summary: | Aims. It has been shown in a number of studies that low temperature leads to significant changes in the organization
of cytoskeleton structures. Recent studies have shed light on the importance of NO, a ubiquitous signaling molecule
in eukaryotes, for plant tolerance to freezing. Methods. In this work we studied the effect of cold and SNP (NO donor)
on organization of actin filaments in the cells of the root seedlings A. thaliana (GFP-ABD2-GFP) using confocal laser
scanning microscope. Results. Observed disorientation and partially depolymerization of actin filaments in the most cells
of the transition zone and individual cells were found. Effect of SNP stimulates restoration of actin filaments; there were
found an extensive network of renewal filaments structures after SNP treatment. Conclusions. Our data have shown for
the first time a negative effect of low temperature on organization and orientation of the actin filaments in various cell
types of the main roots of A. thaliana. We have found that disorientation microfilaments reversed by exogenous donor of
NO, which correlates positively with respect to cold.
Keywords: cytoskeleton, actin filanents, sodium nitroprusside (SNP), green fluorescent protein (GFP), actin binding
domen 2 (ABD2).
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| ISSN: | 2219-3782 |