Аналіз трансгенних рослин кукурудзи та соняшнику з підвищеним рівнем стійкості до водного стресу
Aims. The trustworthy selection of transgenic plants essentially makes fast the whole transformation of procedure. The optimization of mass screening of corn and sunflower plants, obtained after Agrobacterium – mediated transformation in planta was created. Methods. Agrobacterium – mediated transf...
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| Veröffentlicht in: | Фактори експериментальної еволюції організмів |
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| Datum: | 2015 |
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | Ukrainian |
| Veröffentlicht: |
Інститут молекулярної біології і генетики НАН України
2015
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| Schlagworte: | |
| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/177525 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Аналіз трансгенних рослин кукурудзи та соняшнику з підвищеним рівнем стійкості до водного стресу / А.Г. Комісаренко, С.І. Михальська, В.М. Курчій, Л.Є. Сергєєва // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 17. — С. 189-192. — Бібліогр.: 13 назв. — укр. |
Institution
Digital Library of Periodicals of National Academy of Sciences of Ukraine| Zusammenfassung: | Aims. The trustworthy selection of transgenic plants essentially makes fast the whole transformation of procedure. The
optimization of mass screening of corn and sunflower plants, obtained after Agrobacterium – mediated transformation in
planta was created. Methods. Agrobacterium – mediated transformation with pBi2E suppressor of proline dehydrogenase
gene was carried out. T0 corn and sunflower plants were tested under stress pressure. Lethal water stress in vitro was
simulated by the addition of mannitol. The levels of free proline were measured. Results. The transgenic forms survived
under lethal water stress. The free proline levels increased in plant tissues in various manners. The free proline contents
maintained the tolerance and viability of transformed plants. Conclusions. The reliable method of the detection of plants,
resistant to water stress there were proposed.
Keywords: Zea mays, Helianthus annuus, Agrobacterium – mediated transformation in planta, proline dehydrogenase gene
suppressor, water stress, resistance.
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| ISSN: | 2219-3782 |