Генетична трансформація цукрового буряку синтетичним геном cry1C
Aims. Insect pest’s impact makes a significant limitation of the sugar beet crop yield. Integration of cry-genes of Bacillus
 thuringiensis into the plant genome is one of the promising strategies to ensure of plant resistance. The aim of this work was to
 obtain sugar beet lines (ba...
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| Published in: | Фактори експериментальної еволюції організмів |
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| Date: | 2015 |
| Main Authors: | , |
| Format: | Article |
| Language: | Ukrainian |
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Інститут молекулярної біології і генетики НАН України
2015
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/177526 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Генетична трансформація цукрового буряку синтетичним геном cry1C / В.В. Курило, А.І. Ємець // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 17. — С. 193-196. — Бібліогр.: 21 назв. — укр. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862597715365462016 |
|---|---|
| author | Курило, В.В. Ємець, А.І. |
| author_facet | Курило, В.В. Ємець, А.І. |
| citation_txt | Генетична трансформація цукрового буряку синтетичним геном cry1C / В.В. Курило, А.І. Ємець // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 17. — С. 193-196. — Бібліогр.: 21 назв. — укр. |
| collection | DSpace DC |
| container_title | Фактори експериментальної еволюції організмів |
| description | Aims. Insect pest’s impact makes a significant limitation of the sugar beet crop yield. Integration of cry-genes of Bacillus
thuringiensis into the plant genome is one of the promising strategies to ensure of plant resistance. The aim of this work was to
obtain sugar beet lines (based on the MM1/2 line) transformed with cry1C genes using vector construction pRD400-cry1CST.
Methods. Genetic transformation of sugar beet was performed using the procedure of co-cultivation of leaf explants with
Agrobacterium tumefacins. Results. Sugar beet line MM1/2 was transformed by Agrobacterium-mediated transformation
using vector pRD400-cry1CST, containing synthetic cry1C gene and selectable marker gene neomycin phosphotransferase
II (nptII), that conferring resistance to kanamycin. After the optimization protocol of genetic transformation and direct
regeneration from leaf discs a transgenic sugarbeet lines were obtained. They survived on a selective medium containing 1
mg/l benzylaminopurine (BAP), kanamycin as the selective agent in a concentration of 100 mg/l and 250 mg/l cefotaxime
to eliminate excess Agrobacterium. The frequency of transformation in this case was 57.2 %. Conclusions. Based on the
selection and capacity for growth and development in the presence of the selective agent kanamycin, we could suggest that
a target cry1C gene was integrated into the genome of sugar beet plants, that confers resistance to a number of insect genus
of Lepidoptera and Diptera and its expression in transgenic lines of B. vulgaris.
Keywords: genetic transformation, Agrobacterium tumefaciens, Beta vulgaris, cry-genes.
|
| first_indexed | 2025-11-27T19:00:59Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-177526 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 2219-3782 |
| language | Ukrainian |
| last_indexed | 2025-11-27T19:00:59Z |
| publishDate | 2015 |
| publisher | Інститут молекулярної біології і генетики НАН України |
| record_format | dspace |
| spelling | Курило, В.В. Ємець, А.І. 2021-02-16T12:24:19Z 2021-02-16T12:24:19Z 2015 Генетична трансформація цукрового буряку синтетичним геном cry1C / В.В. Курило, А.І. Ємець // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 17. — С. 193-196. — Бібліогр.: 21 назв. — укр. 2219-3782 https://nasplib.isofts.kiev.ua/handle/123456789/177526 62.37:633:6:632.7 Aims. Insect pest’s impact makes a significant limitation of the sugar beet crop yield. Integration of cry-genes of Bacillus
 thuringiensis into the plant genome is one of the promising strategies to ensure of plant resistance. The aim of this work was to
 obtain sugar beet lines (based on the MM1/2 line) transformed with cry1C genes using vector construction pRD400-cry1CST.
 Methods. Genetic transformation of sugar beet was performed using the procedure of co-cultivation of leaf explants with
 Agrobacterium tumefacins. Results. Sugar beet line MM1/2 was transformed by Agrobacterium-mediated transformation
 using vector pRD400-cry1CST, containing synthetic cry1C gene and selectable marker gene neomycin phosphotransferase
 II (nptII), that conferring resistance to kanamycin. After the optimization protocol of genetic transformation and direct
 regeneration from leaf discs a transgenic sugarbeet lines were obtained. They survived on a selective medium containing 1
 mg/l benzylaminopurine (BAP), kanamycin as the selective agent in a concentration of 100 mg/l and 250 mg/l cefotaxime
 to eliminate excess Agrobacterium. The frequency of transformation in this case was 57.2 %. Conclusions. Based on the
 selection and capacity for growth and development in the presence of the selective agent kanamycin, we could suggest that
 a target cry1C gene was integrated into the genome of sugar beet plants, that confers resistance to a number of insect genus
 of Lepidoptera and Diptera and its expression in transgenic lines of B. vulgaris.
 Keywords: genetic transformation, Agrobacterium tumefaciens, Beta vulgaris, cry-genes. uk Інститут молекулярної біології і генетики НАН України Фактори експериментальної еволюції організмів Молекулярні та клітинні біотехнології Генетична трансформація цукрового буряку синтетичним геном cry1C Genetic transformation of sugar beet by synthetic cry1C gene Article published earlier |
| spellingShingle | Генетична трансформація цукрового буряку синтетичним геном cry1C Курило, В.В. Ємець, А.І. Молекулярні та клітинні біотехнології |
| title | Генетична трансформація цукрового буряку синтетичним геном cry1C |
| title_alt | Genetic transformation of sugar beet by synthetic cry1C gene |
| title_full | Генетична трансформація цукрового буряку синтетичним геном cry1C |
| title_fullStr | Генетична трансформація цукрового буряку синтетичним геном cry1C |
| title_full_unstemmed | Генетична трансформація цукрового буряку синтетичним геном cry1C |
| title_short | Генетична трансформація цукрового буряку синтетичним геном cry1C |
| title_sort | генетична трансформація цукрового буряку синтетичним геном cry1c |
| topic | Молекулярні та клітинні біотехнології |
| topic_facet | Молекулярні та клітинні біотехнології |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/177526 |
| work_keys_str_mv | AT kurilovv genetičnatransformacíâcukrovogoburâkusintetičnimgenomcry1c AT êmecʹaí genetičnatransformacíâcukrovogoburâkusintetičnimgenomcry1c AT kurilovv genetictransformationofsugarbeetbysyntheticcry1cgene AT êmecʹaí genetictransformationofsugarbeetbysyntheticcry1cgene |