The positive effect of antibiotic paromomycin compared with kanamycin for selection of transgenic plants with NPTII gene on the example of Nicotiana tabacum

• Опубліковано в: : Фактори експериментальної еволюції організмів
• Дата: 2015
• Автори: Nitovska, I.O., Avilov, I.D., Morgun, B.V.
• Формат: Стаття
• Мова: Англійська
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2015
• Теми: Молекулярні та клітинні біотехнології
• Онлайн доступ: https://nasplib.isofts.kiev.ua/handle/123456789/177534
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: The positive effect of antibiotic paromomycin compared with kanamycin for selection of transgenic plants with NPTII gene on the example of Nicotiana tabacum / I.O. Nitovska, I.D. Avilov, B.V. Morgun // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 17. — С. 270-273. — Бібліогр.: 14 назв. — англ.

Aims. To investigate the efficiency of antibiotic paromomycin as a selective agent for the production of transgenic plants
 with nptII gene compared with kanamycin sulfate on the example of Nicotiana tabacum. Methods. A vector containing
 the nptII gene under the control of 35S promoter of cauliflower mosaic virus was used in Agrobacterium-mediated genetic
 transformation of leaf explants from Nicotiana tabacum. Following parallel explant selection on media with paromomycin
 or kanamycin plant regeneration was observed. Total DNA of the regenerated plants was tested for the presence of nptII
 gene by PCR. Results. It has been shown that the use of antibiotic paromomycin is effective for obtaining transgenic
 tobacco plants. Paromomycin revealed a softer effect on plant explants compared with kanamycin sulfate without causing
 severe depression on regenerative ability of plant tissues, leading to a higher frequency of regeneration on selective
 medium and transformation. Conclusions. Transgenic tobacco plants were received on selective media containing
 paromomycin with high frequency. Due to the mild effects on plant explants, paromomycin is an attractive agent for
 selection of transgenic plants with nptII gene, especially for those species in which the kanamycin sulfate has a strong
 negative impact on the regenerative potential.
 Keywords: Paromomycin, kanamycin, nptII, Nicotiana tabacum, selection of transgenic plants.