Биологически активные вещества перспективных видов Nigella L. и их изменения при обработке электромагнитным излучением

• Veröffentlicht in: Фактори експериментальної еволюції організмів
• Datum: 2016
• Hauptverfasser: Шиш, С.Н., Скаковский, Е.Д., Тычинская, Л.Ю., Шутова, А.Г., Спиридович, Е.В.
• Format: Artikel
• Sprache: Russisch
• Veröffentlicht: Інститут молекулярної біології і генетики НАН України 2016
• Schlagworte: Аналіз та оцінка генетичних ресурсів
• Online Zugang: https://nasplib.isofts.kiev.ua/handle/123456789/177558
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Zitieren: Биологически активные вещества перспективных видов Nigella L. и их изменения при обработке электромагнитным излучением / С.Н. Шиш, Е.Д. Скаковский, Л.Ю. Тычинская, А.Г. Шутова, Е.В. Спиридович // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2016. — Т. 18. — С. 211-214. — Бібліогр.: 5 назв. — рос.

Aim. The evaluation of biologically active compounds of seeds of three Nigella species cultivated in Belarus and their
 changes under the influence of electromagnetic radiation. Methods. A comparative NMR analysis of chloroform extracts
 of three species of the Nigella family (Nigella damascena L., Nigella sativa L., Nigella orientalis L.) has been carried
 out and the influence of EMR on the extract composition has been investigated. The seeds were obtained from plants
 cultivated on the experimental site of the Department of Plant Biochemistry and Biotechnology of the Central Botanical
 Garden, NAS of Belarus. Results. Fatty acid composition of the extract was evaluated; linoleic and oleic acids are the
 most abundant among triglycerides. The presence of thymoquinone in the extracts was proven. The content of unsaturated
 fatty acids in the lipid fraction of the Nigella sativa seeds increases by 10–13% after the treatment with electromagnetic
 radiation in Regime 1 (53.57–78.33 GHz, 20 min) and Regime 3 (64.0–66.0 GHz, 8 min). The influence of EMR on the
 presence of thymoquinone in the seeds is shown. Conclusions. The variation of fatty acids composition of three different
 Nigella species has been shown. The specificity of N. damascena is the highest content of eicosadienoic acid in the seed
 extracts, while those of N. orientalis contains the highest amount of linoleic acid. N. sativa is the leader in thymoquinone
 accumulation.The changes of fatty acids composition and structure of N. sativa triacylglycerides that occur under the
 influence of EMR are determined.
 Keywords: seeds, Nigella sativa, Nigella damascena, Nigella orientalis, nuclear magnetic resonance, fatty acids.