Анализ связи фрагментации ДНК и анеуплоидий спермы с параметрами ранних эмбрионов

• Published in: Фактори експериментальної еволюції організмів
• Date: 2016
• Main Authors: Жилкова, Е.С., Чумакова, Н.А., Тищенко, А.А., Феськов, А.М., Федота, А.М.
• Format: Article
• Language: Russian
• Published: Інститут молекулярної біології і генетики НАН України 2016
• Subjects: Генетика людини та медична генетика
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/177621
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Анализ связи фрагментации ДНК и анеуплоидий спермы с параметрами ранних эмбрионов / Е.С. Жилкова, Н.А. Чумакова, А.А. Тищенко, А.М. Феськов, А.М. Федота // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2016. — Т. 18. — С. 231-235. — Бібліогр.: 28 назв. — рос.

Aim. The dependence of the process of blastocyst formation in IVF practice on the level of sperm DNA fragmentation
 and sperm aneuploidies in men with low reproductive function was studied. Methods. The level of the sperm DNA
 fragmentation was measured by the method of sperm chromatin dispersion. Semen aneuploidies were detected by the
 method of fluorescence in situ hybridization. Results. The significant negative correlation between DNA fragmentation
 level and formation of the high quality blastocyst is proved. There is a negative correlation between the level sperm
 aneuploidies and number and quality of formatted blastocysts. Conclusions. The failures of sperm DNA compactization
 influence the early embryo development. There is a dependence of blastocyst formation rates on the level of sperm
 aneuploidy.
 Keywords: DNA fragmentation, sperm aneuploidy, blastocyst formation rates.