Вплив делеції гена β-катеніну на морфологію та фізіологію кардіоміоцитів за умов дії стимуляторів гіпертрофії
Aim. The investigation of β-catenin signaling function during cardiomyocyte hypertrophy development under the one
 allele of β-catenin conditional cardiospecific knock-out condition was the aim of present work. Methods. The heart
 of new-born trangenic and wild-type animals were used...
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| Veröffentlicht in: | Фактори експериментальної еволюції організмів |
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| Datum: | 2016 |
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | Ukrainisch |
| Veröffentlicht: |
Інститут молекулярної біології і генетики НАН України
2016
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| Schlagworte: | |
| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/177643 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Вплив делеції гена β-катеніну на морфологію та фізіологію кардіоміоцитів за умов дії стимуляторів гіпертрофії / О.Л. Пальчевська, А.А. Хазєєва, Н.В. Мачушинець, Т.П. Рубан, Л.Л. Мацевич, О.О. Півень // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2016. — Т. 18. — С. 242-247. — Бібліогр.: 27 назв. — укр. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Zusammenfassung: | Aim. The investigation of β-catenin signaling function during cardiomyocyte hypertrophy development under the one
allele of β-catenin conditional cardiospecific knock-out condition was the aim of present work. Methods. The heart
of new-born trangenic and wild-type animals were used for the primary culture isolation. The physiological activity
was evaluated with MTT-test. The primary cardiomyocytes were H&E stained and morphological parameters were
measured. Results. The physiological activity elevation was detected for wild-type and haploinsufficient animals after
lithium chloride treatment comparing to control cells in MTT-test. The inhibition of physiological activity was detected
for all genotypes comparing to controls. The binucleated cells distribution was shown to differ between the control and
LiCl-treated cells. Also the frequency of binucleated cells tended to increase for mutants comparing to controls after
canonical Wnt stimulation with LiCl. Conclusions. The deletion of β-catenin leads to disruptions in cell morphology
and physiology in postnatal cardiovyocytes and attenuated cell hypertrophy development possibly due to canonical Wntsignaling inhibition.
Keywords: hypertrophic stimuli, β-catenin haploinsufficiency, β-catenin deletion, canonical Wnt-signaling.
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| ISSN: | 2219-3782 |