Молекулярные маркеры для селекции новых сортов гороха, устойчивых к мучнистой росе

Aims. Powdery mildew is economically important disease of pea (Pisum sativum L.) as it causes severe losses of yield worldwide. Environmental friendly approach to control powdery mildew, in contrast to chemical protection, is use of the resistant cultivars. Mutations in pea gene PsMLO1 that confer r...

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Veröffentlicht in:Фактори експериментальної еволюції організмів
Datum:2013
Hauptverfasser: Жуков, В.А., Сулима, А.С., Жернаков, А.И., Штарк, О.Ю., Борисов, А.Ю., Тихонович, И.А.
Format: Artikel
Sprache:Russian
Veröffentlicht: Інститут молекулярної біології і генетики НАН України 2013
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Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/177751
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Zitieren:Молекулярные маркеры для селекции новых сортов гороха, устойчивых к мучнистой росе / В.А. Жуков, А.С. Сулима, А.И. Жернаков, О.Ю. Штарк, А.Ю. Борисов, И.А. Тихонович // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 173-177. — Бібліогр.: 10 назв. — рос.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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Zusammenfassung:Aims. Powdery mildew is economically important disease of pea (Pisum sativum L.) as it causes severe losses of yield worldwide. Environmental friendly approach to control powdery mildew, in contrast to chemical protection, is use of the resistant cultivars. Mutations in pea gene PsMLO1 that confer resistance to powdery mildew can be used as molecular markers for breeding resistant cultivars. Methods. Mutant allelic variants of PsMLO1 were sequenced in resistant pea cultivars and lines in order to detect SNPs and/or indels suitable for creation PCR-based molecular markers. Results. The system of 3 primers was designed that allows one-step PCR-based identification of natural mutant allele PsMLO1 with transposon insertion in reading frame that presents in resistant pea cultivar Franklin. Conclusions. This molecular marker can be used for breeding resistant cultivars when taking cultivar Franklin (as well as related lines and cultivars) as donors of powdery mildew resistance trait. Key words: Pisum sativum L., powdery mildew, molecular markers, resistance, breeding.
ISSN:2219-3782