Молекулярные маркеры для селекции новых сортов гороха, устойчивых к мучнистой росе
Aims. Powdery mildew is economically important disease of pea (Pisum sativum L.) as it causes severe losses of yield worldwide. Environmental friendly approach to control powdery mildew, in contrast to chemical protection, is use of the resistant cultivars. Mutations in pea gene PsMLO1 that confer r...
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| Date: | 2013 |
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Інститут молекулярної біології і генетики НАН України
2013
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| Cite this: | Молекулярные маркеры для селекции новых сортов гороха, устойчивых к мучнистой росе / В.А. Жуков, А.С. Сулима, А.И. Жернаков, О.Ю. Штарк, А.Ю. Борисов, И.А. Тихонович // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 173-177. — Бібліогр.: 10 назв. — рос. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1860081441205911552 |
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| author | Жуков, В.А. Сулима, А.С. Жернаков, А.И. Штарк, О.Ю. Борисов, А.Ю. Тихонович, И.А. |
| author_facet | Жуков, В.А. Сулима, А.С. Жернаков, А.И. Штарк, О.Ю. Борисов, А.Ю. Тихонович, И.А. |
| citation_txt | Молекулярные маркеры для селекции новых сортов гороха, устойчивых к мучнистой росе / В.А. Жуков, А.С. Сулима, А.И. Жернаков, О.Ю. Штарк, А.Ю. Борисов, И.А. Тихонович // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 173-177. — Бібліогр.: 10 назв. — рос. |
| collection | DSpace DC |
| container_title | Фактори експериментальної еволюції організмів |
| description | Aims. Powdery mildew is economically important disease of pea (Pisum sativum L.) as it causes severe losses of yield worldwide. Environmental friendly approach to control powdery mildew, in contrast to chemical protection, is use of the resistant cultivars. Mutations in pea gene PsMLO1 that confer resistance to powdery mildew can be used as molecular markers for breeding resistant cultivars. Methods. Mutant allelic variants of PsMLO1 were sequenced in resistant pea cultivars and lines in order to detect SNPs and/or indels suitable for creation PCR-based molecular markers. Results. The system of 3 primers was designed that allows one-step PCR-based identification of natural mutant allele PsMLO1 with transposon insertion in reading frame that presents in resistant pea cultivar Franklin. Conclusions. This molecular marker can be used for breeding resistant cultivars when taking cultivar Franklin (as well as related lines and cultivars) as donors of powdery mildew resistance trait.

Key words: Pisum sativum L., powdery mildew, molecular markers, resistance, breeding.
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| first_indexed | 2025-12-07T17:17:01Z |
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173
YEMETS Z.V., MAMENKO O.M., KHRUCKIY S.S.
Kharkov state zooveterinary Academy, The Ministry Of Agrarian Of Ukraine
Ukraine, 62341, Kharkov, Small Danilovka, e-mail: zoya_emez@mail.ru
CHANGES THE PROTEIN PERCENTAGE OF COWS UNDER THE INFLUENCE OF
NEGATIVE FACTORS BIOGEOCHEMICAL PROVINCE
Aims. In the article the results of scientific and business experience, which was held in the zone of heavy
metals contamination of the biogeochemical province in the negative anthropogenic impact on genetically
predetermined quality indicator of protein in the milk of cows. Given production processing method for the
production of environmentally safe milk and increasing percentage of protein in the milk of cows with the
help of toxic action of mineral additives and biologically active preparation «AVGOR-5». Methods. Labora-
tory of physico-chemical tests of an experimental material with use of the method of atomic-absorption spec-
trophotometry S-30 (Carl Zeiss, Jena), held – biometric processing of the received results. Results. It
should be noted that due to the application of special fodder additives and biologically active preparation was
achieved not only the persistence of high levels of protein content in the milk, but also a decrease in urinary
excretion of extremely toxic creatinine, which is important for prevention of environmental pollution. Con-
clusions. Genetically predetermined rate the protein percentage milk may change under the influence of such
«shock» factor, as the high content of heavy metals in the feed. A balanced vitamin-mineral additives, used
in the feeding of the cows with the product «AVGOR-5», contributed to the normalization of such genetic
indicator, as the content of protein in the milk of cows, which in connection with the anthropogenic pollution
is significantly decline in province of the Central Donbass with the negative anthropogenic influence that has
affected the quality of the products obtained, and may be evidence of the oppression of genetically caused by
the capacity of animals in the protein percentage.
Key words: protein in the milk of cows, genetically predetermined figure, the biogeochemical province,
negative factors.
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protein-coupled receptors, GPCR) [1].
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(Mike Ambrose, John
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toulouse.prd.fr/multalin/multalin.html) ClustalW2
(http://www.ebi.ac.uk/Tools/clustalw2/index.html),
MEGA 5.05 [8].
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,
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(Retrotransposon-Based Insertion Polymorphisms),
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,
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);
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176
. PsMLO1 ,
(Franklin) (SGE, )
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.
,
( ,
2012147570/20(076429)).
(
)
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( ,
)
( .).
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-
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. ( -
337.2012.4), ( 16.512.11.2155 8056
8109) (12-04-01687- , 12-04-32126_ -a, 13-04-01702- 13-04-01703- ).
1. Devoto A., Hartmann H.A., Piffanelli P., Elliott C., Simmons C., Taramino G., Goh C.-S., Cohen F.E., Emerson
B.C., Schulze-Lefert P. Molecular phylogeny and evolution of the plant-specific seven-transmembrane MLO
family // J. of Mol. Evol. – 2003. – Vol. 56, 1. – P. 77–88.
2. Humphry M., Reinstädler A., Ivanov S., Bisseling T., Panstruga R. Durable broad-spectrum powdery mildew
resistance in pea er1 plants is conferred by natural loss-of-function mutations in PsMLO1 // Mol. Plant Pathol. –
2011. – Vol. 12, 9. – P. 866–878.
3. Kibbe WA. OligoCalc: an online oligonucleotide properties calculator // Nucleic Acids Res. – 2007. – Vol. 35
177
(webserver issue). – W. 43–46.
4. Mahmood T., Ahmad I., Qureshi S., Aslam M. Estimation of yield losses due to powdery mildew in peas // Pak. J.
Bot. – 1983. – Vol. 15. – P. 113–115.
5. Pavan S., Schiavulli A., Appiano M., Marcotrigiano A.R., Cillo F., Visser R.G., Bai Y., Lotti C., Ricciardi L. Pea
powdery mildew er1 resistance is associated to loss-of-function mutations at a MLO homologous locus // Theor.
Appl. Genet. – 2011. – Vol. 123, 8. – P. 1425–1431.
6. Pavan S., Schiavulli A., Appiano M., Miacola C., Visser R.F., Bai Y., Lotti C., Ricciardi L. Identification of a
complete set of functional markers for the selection of er1 powdery mildew resistance in Pisum sativum L. // Mol.
Breed. – 2013. – Vol. 31, 1. – P. 247–253.
7. Rogers S.O., Bendich A.J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant
tissues // Plant Mol. Biol. – 1985. – Vol. 5. – P. 69–76.
8. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. MEGA5: molecular evolutionary genetics
analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods // Mol. Biol. Evol. –
2011. – Vol. 28, 10. – P. 2731–2739.
9. Tiwari K., Penner G., Warkentin T. Inheritance of powdery mildew resistance in pea // Can. J. Plant Sci. – 1997. –
Vol. 77, 3. – P. 307–310.
10. Untergasser A., Nijveen H., Rao X., Bisseling T., Geurts R., Leunissen J.A.M. Primer3Plus, an enhanced web
interface to Primer3 // Nucleic Acids Res. – 2007. – Vol. 35 (webserver issue). – W. 71–74.
ZHUKOV V.A., SULIMA A.S., ZHERNAKOV A.I., SHTARK O.Y., BORISOV A.Y.,
TIKHONOVICH I.A.
All-Russia Research Institute for Agricultural Microbiology RAAS
Russia, 196608, Saint-Petersburg, Pushkin, Podbelsky ch., 3, e-mail: zhukoff01@yahoo.com
MOLECULAR MARKERS FOR BREEDING THE NEW PEA CULTIVARS RESISTANT TO
POWDERY MILDEW
Aims. Powdery mildew is economically important disease of pea (Pisum sativum L.) as it causes severe
losses of yield worldwide. Environmental friendly approach to control powdery mildew, in contrast to
chemical protection, is use of the resistant cultivars. Mutations in pea gene PsMLO1 that confer resistance to
powdery mildew can be used as molecular markers for breeding resistant cultivars. Methods. Mutant allelic
variants of PsMLO1 were sequenced in resistant pea cultivars and lines in order to detect SNPs and/or indels
suitable for creation PCR-based molecular markers. Results. The system of 3 primers was designed that
allows one-step PCR-based identification of natural mutant allele PsMLO1 with transposon insertion in
reading frame that presents in resistant pea cultivar Franklin. Conclusions. This molecular marker can be
used for breeding resistant cultivars when taking cultivar Franklin (as well as related lines and cultivars) as
donors of powdery mildew resistance trait.
Key words: Pisum sativum L., powdery mildew, molecular markers, resistance, breeding.
. .
. . . ’
, 61060, , ., 142, e-mail:olzador@ukr.net
, QTL,
.
70 %.
-
. 2011
8,7 , 28 %
2005 [1].
9 %
, . -
,
. -
.
2012
(USDA) -
57 %, -
.
11 % ,
|
| id | nasplib_isofts_kiev_ua-123456789-177751 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 2219-3782 |
| language | Russian |
| last_indexed | 2025-12-07T17:17:01Z |
| publishDate | 2013 |
| publisher | Інститут молекулярної біології і генетики НАН України |
| record_format | dspace |
| spelling | Жуков, В.А. Сулима, А.С. Жернаков, А.И. Штарк, О.Ю. Борисов, А.Ю. Тихонович, И.А. 2021-02-16T16:26:14Z 2021-02-16T16:26:14Z 2013 Молекулярные маркеры для селекции новых сортов гороха, устойчивых к мучнистой росе / В.А. Жуков, А.С. Сулима, А.И. Жернаков, О.Ю. Штарк, А.Ю. Борисов, И.А. Тихонович // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 173-177. — Бібліогр.: 10 назв. — рос. 2219-3782 https://nasplib.isofts.kiev.ua/handle/123456789/177751 Aims. Powdery mildew is economically important disease of pea (Pisum sativum L.) as it causes severe losses of yield worldwide. Environmental friendly approach to control powdery mildew, in contrast to chemical protection, is use of the resistant cultivars. Mutations in pea gene PsMLO1 that confer resistance to powdery mildew can be used as molecular markers for breeding resistant cultivars. Methods. Mutant allelic variants of PsMLO1 were sequenced in resistant pea cultivars and lines in order to detect SNPs and/or indels suitable for creation PCR-based molecular markers. Results. The system of 3 primers was designed that allows one-step PCR-based identification of natural mutant allele PsMLO1 with transposon insertion in reading frame that presents in resistant pea cultivar Franklin. Conclusions. This molecular marker can be used for breeding resistant cultivars when taking cultivar Franklin (as well as related lines and cultivars) as donors of powdery mildew resistance trait.
 
 Key words: Pisum sativum L., powdery mildew, molecular markers, resistance, breeding. ru Інститут молекулярної біології і генетики НАН України Фактори експериментальної еволюції організмів Прикладна генетика і селекція Молекулярные маркеры для селекции новых сортов гороха, устойчивых к мучнистой росе Molecular markers for breeding the new pea cultivars resistant to powdery mildew Article published earlier |
| spellingShingle | Молекулярные маркеры для селекции новых сортов гороха, устойчивых к мучнистой росе Жуков, В.А. Сулима, А.С. Жернаков, А.И. Штарк, О.Ю. Борисов, А.Ю. Тихонович, И.А. Прикладна генетика і селекція |
| title | Молекулярные маркеры для селекции новых сортов гороха, устойчивых к мучнистой росе |
| title_alt | Molecular markers for breeding the new pea cultivars resistant to powdery mildew |
| title_full | Молекулярные маркеры для селекции новых сортов гороха, устойчивых к мучнистой росе |
| title_fullStr | Молекулярные маркеры для селекции новых сортов гороха, устойчивых к мучнистой росе |
| title_full_unstemmed | Молекулярные маркеры для селекции новых сортов гороха, устойчивых к мучнистой росе |
| title_short | Молекулярные маркеры для селекции новых сортов гороха, устойчивых к мучнистой росе |
| title_sort | молекулярные маркеры для селекции новых сортов гороха, устойчивых к мучнистой росе |
| topic | Прикладна генетика і селекція |
| topic_facet | Прикладна генетика і селекція |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/177751 |
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