Молекулярно-генетические эффекты дупликации генома у ржи (Secale cerealе L.)
Aims. Studying of the molecular-genetic effects of genome duplication in winter rye on cellular, protein and DNA levels. Methods. Cytologic analysis of the chromosome number, the microsporogenesis, karyotype analysis with use C-banding, electrophoresis of the storage proteins - secalins, PCR-analysi...
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| Veröffentlicht in: | Фактори експериментальної еволюції організмів |
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| Datum: | 2013 |
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | Russisch |
| Veröffentlicht: |
Інститут молекулярної біології і генетики НАН України
2013
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| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/177755 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Молекулярно-генетические эффекты дупликации генома у ржи (Secale cerealе L.) / И.С. Гордей, Н.Б. Белько, И.А. Гордей // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 156-161. — Бібліогр.: 8 назв. — рос. |
Institution
Digital Library of Periodicals of National Academy of Sciences of Ukraine| Zusammenfassung: | Aims. Studying of the molecular-genetic effects of genome duplication in winter rye on cellular, protein and DNA levels. Methods. Cytologic analysis of the chromosome number, the microsporogenesis, karyotype analysis with use C-banding, electrophoresis of the storage proteins - secalins, PCR-analysis with random primers (RAPD). Results. Duplication of the chromosome number in winter rye accompanied by a significant infringements of the microsporogenesis process, changes in the spectrum of amplified DNA fragments and the spectra of species-specific proteins of seeds (secalins). Conclusions. Duplication of the genome in rye leads to multiple molecular genetic effects on cellular, protein and DNA levels, due to an infrigements of a balanced genetic system of meiosis control in diploid plants, gene expression changes in species-specific seed proteins and structural changes in DNA as a result of duplication.

Key words: winter rye, genome duplication, chromosomes, DNA, aneuploidy, meiosis, polymorphism, electrophoresis, PCR-analysis.
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| ISSN: | 2219-3782 |