Молекулярно-генетические эффекты дупликации генома у ржи (Secale cerealе L.)

• Published in: Фактори експериментальної еволюції організмів
• Date: 2013
• Main Authors: Гордей, И.С., Белько, Н.Б., Гордей, И.А.
• Format: Article
• Language: Russian
• Published: Інститут молекулярної біології і генетики НАН України 2013
• Subjects: Прикладна генетика і селекція
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/177755
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Молекулярно-генетические эффекты дупликации генома у ржи (Secale cerealе L.) / И.С. Гордей, Н.Б. Белько, И.А. Гордей // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 156-161. — Бібліогр.: 8 назв. — рос.

Aims. Studying of the molecular-genetic effects of genome duplication in winter rye on cellular, protein and DNA levels. Methods. Cytologic analysis of the chromosome number, the microsporogenesis, karyotype analysis with use C-banding, electrophoresis of the storage proteins - secalins, PCR-analysis with random primers (RAPD). Results. Duplication of the chromosome number in winter rye accompanied by a significant infringements of the microsporogenesis process, changes in the spectrum of amplified DNA fragments and the spectra of species-specific proteins of seeds (secalins). Conclusions. Duplication of the genome in rye leads to multiple molecular genetic effects on cellular, protein and DNA levels, due to an infrigements of a balanced genetic system of meiosis control in diploid plants, gene expression changes in species-specific seed proteins and structural changes in DNA as a result of duplication. Key words: winter rye, genome duplication, chromosomes, DNA, aneuploidy, meiosis, polymorphism, electrophoresis, PCR-analysis.