Зв’язок стабільності геному з різними генотипами генів відтворення свиноматок

Зв’язок стабільності геному з різними генотипами генів відтворення свиноматок

Aim. The aim of this study was to investigate the association of genotypes communication complex genes reproductive capacity of sows genome and identify the most promising in terms of increasing bahatoplidnosti studied animal populations combination. Methods. Studies were conducted by using standard...

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Veröffentlicht in:Фактори експериментальної еволюції організмів
Datum:2013
Hauptverfasser: Драгулян, М.В., Костенко, С.О., Сидоренко, О.В.
Format: Artikel
Sprache:Ukrainian
Veröffentlicht: Інститут молекулярної біології і генетики НАН України 2013
Schlagworte:
Прикладна генетика і селекція
Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/177758
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Zitieren:Зв’язок стабільності геному з різними генотипами генів відтворення свиноматок / М.В. Драгулян, С.О. Костенко, О.В. Сидоренко // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 166-170. — Бібліогр.: 5 назв. — укр.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-177758
record_format dspace
spelling Драгулян, М.В.
Костенко, С.О.
Сидоренко, О.В.
2021-02-16T16:28:14Z
2021-02-16T16:28:14Z
2013
Зв’язок стабільності геному з різними генотипами генів відтворення свиноматок / М.В. Драгулян, С.О. Костенко, О.В. Сидоренко // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 166-170. — Бібліогр.: 5 назв. — укр.
2219-3782
https://nasplib.isofts.kiev.ua/handle/123456789/177758
Aim. The aim of this study was to investigate the association of genotypes communication complex genes reproductive capacity of sows genome and identify the most promising in terms of increasing bahatoplidnosti studied animal populations combination. Methods. Studies were conducted by using standard molecular genetic and cytogenetic methods. In cytogenetic preparations take into account the level of cells with micronuclei (MN), dual (AH) and apoptotic (AP) cells, and mitotic index (MI). The study of gene polymorphism ESR, NCOA1, PRLR, was performed by PCR–RFLP. FSHR gene polymorphism was performed method Bi-Passa (without restriction). Results. Detected frequencies of alleles and genotypes of genes FSHR, NCOA1, ESR, PRLR and the animals Ukrainian meat and Welsh breeds. Advantage of sows Ukrainian meat and Welsh breeds certain genotypes over their counterparts. A cytogenetic testing sows and found that the frequency of cells with micronuclei affects the twins and the percentage of emergency farrowing. In studying the stability of the genome due to different genotypes of genes reproductive capacity of sows was found that both sows carrier all 4 desired alleles of both species even at high levels of cells with micronuclei is a high percentage of twins and preservation of offspring. Conclusions. Pattern revealed positive effects of alleles ESRB, NCOA1A1, PRLRA, FSHRC. The authentic correlation between productivity performance and the level of micronuclei animals suggests that animals should be selected not only based on DNA markers, but you should still take into account the stability of the genome of pigs. More stable gene was observed in animals with desirable and intermediate genotypes to genes ESR/NCOA1/PRLR/FSHR. Key words: gene receptor gene, lymphocyte, sow, multiple.
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Інститут молекулярної біології і генетики НАН України
Фактори експериментальної еволюції організмів
Прикладна генетика і селекція
Зв’язок стабільності геному з різними генотипами генів відтворення свиноматок
Relationship with stability genome of different genotypes genes play sows
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Зв’язок стабільності геному з різними генотипами генів відтворення свиноматок
spellingShingle Зв’язок стабільності геному з різними генотипами генів відтворення свиноматок
Драгулян, М.В.
Костенко, С.О.
Сидоренко, О.В.
Прикладна генетика і селекція
title_short Зв’язок стабільності геному з різними генотипами генів відтворення свиноматок
title_full Зв’язок стабільності геному з різними генотипами генів відтворення свиноматок
title_fullStr Зв’язок стабільності геному з різними генотипами генів відтворення свиноматок
title_full_unstemmed Зв’язок стабільності геному з різними генотипами генів відтворення свиноматок
title_sort зв’язок стабільності геному з різними генотипами генів відтворення свиноматок
author Драгулян, М.В.
Костенко, С.О.
Сидоренко, О.В.
author_facet Драгулян, М.В.
Костенко, С.О.
Сидоренко, О.В.
topic Прикладна генетика і селекція
topic_facet Прикладна генетика і селекція
publishDate 2013
language Ukrainian
container_title Фактори експериментальної еволюції організмів
publisher Інститут молекулярної біології і генетики НАН України
format Article
title_alt Relationship with stability genome of different genotypes genes play sows
description Aim. The aim of this study was to investigate the association of genotypes communication complex genes reproductive capacity of sows genome and identify the most promising in terms of increasing bahatoplidnosti studied animal populations combination. Methods. Studies were conducted by using standard molecular genetic and cytogenetic methods. In cytogenetic preparations take into account the level of cells with micronuclei (MN), dual (AH) and apoptotic (AP) cells, and mitotic index (MI). The study of gene polymorphism ESR, NCOA1, PRLR, was performed by PCR–RFLP. FSHR gene polymorphism was performed method Bi-Passa (without restriction). Results. Detected frequencies of alleles and genotypes of genes FSHR, NCOA1, ESR, PRLR and the animals Ukrainian meat and Welsh breeds. Advantage of sows Ukrainian meat and Welsh breeds certain genotypes over their counterparts. A cytogenetic testing sows and found that the frequency of cells with micronuclei affects the twins and the percentage of emergency farrowing. In studying the stability of the genome due to different genotypes of genes reproductive capacity of sows was found that both sows carrier all 4 desired alleles of both species even at high levels of cells with micronuclei is a high percentage of twins and preservation of offspring. Conclusions. Pattern revealed positive effects of alleles ESRB, NCOA1A1, PRLRA, FSHRC. The authentic correlation between productivity performance and the level of micronuclei animals suggests that animals should be selected not only based on DNA markers, but you should still take into account the stability of the genome of pigs. More stable gene was observed in animals with desirable and intermediate genotypes to genes ESR/NCOA1/PRLR/FSHR. Key words: gene receptor gene, lymphocyte, sow, multiple.
issn 2219-3782
url https://nasplib.isofts.kiev.ua/handle/123456789/177758
citation_txt Зв’язок стабільності геному з різними генотипами генів відтворення свиноматок / М.В. Драгулян, С.О. Костенко, О.В. Сидоренко // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 166-170. — Бібліогр.: 5 назв. — укр.
work_keys_str_mv AT dragulânmv zvâzokstabílʹnostígenomuzríznimigenotipamigenívvídtvorennâsvinomatok
AT kostenkoso zvâzokstabílʹnostígenomuzríznimigenotipamigenívvídtvorennâsvinomatok
AT sidorenkoov zvâzokstabílʹnostígenomuzríznimigenotipamigenívvídtvorennâsvinomatok
AT dragulânmv relationshipwithstabilitygenomeofdifferentgenotypesgenesplaysows
AT kostenkoso relationshipwithstabilitygenomeofdifferentgenotypesgenesplaysows
AT sidorenkoov relationshipwithstabilitygenomeofdifferentgenotypesgenesplaysows
first_indexed 2025-11-24T16:27:43Z
last_indexed 2025-11-24T16:27:43Z
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fulltext 166 1. - , . 2. - . - - « », « » « » . « / » - . 3. – - - « ». , - – . . 1. . ., . ., . . - . – / . . . 15.11.1987. 138687 2. . . . – : , 2008. – . . – 151 . 3. Hammond G.T. and Mahlbery P.G. Morphology of Clangular Nairs of Cannabis sativa from scanning electron microscopy / G.T. Hammond American Journal of Botany. – 1973. – Vol. 60, 6. – P. 524–528. HORSHKOVA L.M.1, BOHDANOVA A.S.1, VYROVETS V.H.2 1.Hlukhiv National Pedagogical University named by Oleksandr Dovzhenko Ukraine, 41400, Sumy region, Hlukhiv, Kyiv-Moskow Street, 24, -mail: kafbiol@mail.ru 2.Research Station of Bast Crops of the Institute of Agriculture of Northern-East NAAS Ukraine, 41400, Sumy region, Hlukhiv, Tereschenkiv Street, 45, -mail: ibc@sm.ukrtel.net INHERITING THE GLAND HAIRS MORPHOLOGICAL FEATURES BY THE POSTERITY Aims. The article deals with the research of inheriting the morphological features of gland hairs by the posterity. The connection between the existence of the gland hairs and the concentration of cannabinoids in the hemp plants was investigated. The selective work was aimed at lowering the cannabinoids content in the hemp plants by means of selecting the plants with less gland hairs as they are mainly the bearers of the substance. Methods. During the investigation the stereoscopic microscope was the main device and the method of the thin layer chromatography was used. The cannabinoids quantity was defined by the gas and liquid chromatograph of the type Hewlett Packard 3380 A. The inner standard of the experiment was methyl ether of the stearin acid C19H38O2. Results. The second generation hybrids demonstrated splitting by the phenotype. The hybridological analysis proved dominating the gland hairs over their absence. Conclusions. Besides the interaction of two dominant genes was shown as the new morphological feature - the gland form which was the proof of the genes complementary result. Key words: lowering the cannabinoids content, gland hairs, inheriting, morphological features, thin layer chromatography method. . . 1, . . 2, . . 3 1 , 03143, , . . , 150, e-mail: parus_major@ukr.net 2 , 03041, , . , 15, e-mail: swetakostenko@mail.ru 3 , 08321, ., . , . , 1 ’ , (ES) - (PRL) - (FSH) , , , 167 , , - , , [4]. - - . - - , - [1], , - [4]. : - (ESR), (NCOA1), - (PRLR), - (FSHR) , - . - . - , - . . . ’ ’ ’ (n=73) (n=123) , « » - , . « - » - . ESR, NCOA1, PRLR, – . FSHR - Bi-Passa ( ). ESR, NCOA1, PRLR - – PvuII, RsaI, AluI. 2 % . , - - ( AA ) . [3]. ( ), ( ) ( ) - , ( ). 1000 . 3000 . Excel 2003. - - ’ - ESR, NCOA1, PRLR, FSHR ( . 1). - - ESRB, NCOA1A1, PRLRA, FSHRC ( .). - ’ [1, 4]. 1. ’ ’ 0,48±0,028 0,40±0,021 ESR 0,52±0,027 0,60±0,026 0,001 1 0,62±0,020 0,66±0,020 NCOA1 2 0,38±0,030 0,34±0,027 0,001 0,58±0,019 0,53±0,016 PRLR 0,42±0,022 0,47±0,017 0,001 0,73±0,018 0,75±0,015 FSHR L 0,27±0,030 0,25±0,026 – 168 , - - ( ). - - . , , , - . - ESRBB/FSHRCC , - ’ - ESR /PRLR . , - NCOA1A1A1/ESR /FSHR . NCOA1A1A1/ESR /FSHR /PRLR , - NCOA1A2A2/ESR /FSHR /PRLR 4,4 2,0 ’ . , , . . ’ - - . , - - ’ - 0,67 ‰ 1,9 ‰ . ' - - ( 0,9 ‰). - , ( <0,01). - ( . 2). ’ - . ’ : r=0,57 ( <0,01) - r=0,35 ( <0,01) ’ . , - . ’ - , - . . [5]. 169 2. ’ , ‰ , ‰ , ‰ , ‰ ’ 3,64±0,28** 2,33±0,26 4,26±0,38** 4,09±0,33** 4,58±0,21 2,60±0,21 3,11±0,26 5,56±0,32 . ** <0,01 . - , - - , ' - . - 4 - . - , – - - ( . 3). 3. ’ 5144 7 11 89,00 342 6,7 10 90,00 700 6,2 12,5 88,00 1090 5,8 5 0,00 28 5,9 5 10,00 230 7,6 8 77,7 , - - , , - , [2]. , - , , - , , - , . ESRB, NCOA1A1, PRLRA, FSHRC. - , - , - . ESR/NCOA1/PRLR/FSHR. 1. . . : - . . . . : . 03.02.21. – / . – ., 2005. – 24 . 2. . ., . . // . – 2004. – . 46, 2. – . 105–113. 3. - / . ., . ., . ., . . // . . . « ». – : AA , 1995. – . 17. 4. . . , // i ii AB i. – 2008. – 2. – . 81–85. 5. . . - : . . . : 06.02.01. – , , / . – . . . : - - . – ., 1992. – 113 . 170 DRAHULYAN M.V. 1, KOSTENKO S.O. 2, SYDORENKO O.V. 3 1 Institute of Molecular Biology and Genetics NAS of Ukraine Ukraine, 03143, Kyiv, Akad. Zabolotnoho, 150, e-mail: parus_major@ukr.net 2 Natsionalnyy University of Life and Environmental Sciences of Ukraine Ukraine, 03041, Kyiv, Heroes of Defense str., 15, e-mail: swetakostenko@mail.ru 3 Institute of Animal Breeding and Genetics NAAS of Ukraine Ukraine, 08321, Kyiv region, v. Chubinskoe, Pogrebnyak str., 1 RELATIONSHIP WITH STABILITY GENOME OF DIFFERENT GENOTYPES GENES PLAY SOWS Aim. The aim of this study was to investigate the association of genotypes communication complex genes reproductive capacity of sows genome and identify the most promising in terms of increasing bahatoplidnosti studied animal populations combination. Methods. Studies were conducted by using standard molecular genetic and cytogenetic methods. In cytogenetic preparations take into account the level of cells with micronuclei (MN), dual (AH) and apoptotic (AP) cells, and mitotic index (MI). The study of gene polymorphism ESR, NCOA1, PRLR, was performed by PCR–RFLP. FSHR gene polymorphism was performed method Bi-Passa (without restriction). Results. Detected frequencies of alleles and genotypes of genes FSHR, NCOA1, ESR, PRLR and the animals Ukrainian meat and Welsh breeds. Advantage of sows Ukrainian meat and Welsh breeds certain genotypes over their counterparts. A cytogenetic testing sows and found that the frequency of cells with micronuclei affects the twins and the percentage of emergency farrowing. In studying the stability of the genome due to different genotypes of genes reproductive capacity of sows was found that both sows carrier all 4 desired alleles of both species even at high levels of cells with micronuclei is a high percentage of twins and preservation of offspring. Conclusions. Pattern revealed positive effects of alleles ESRB, NCOA1A1, PRLRA, FSHRC. The authentic correlation between productivity performance and the level of micronuclei animals suggests that animals should be selected not only based on DNA markers, but you should still take into account the stability of the genome of pigs. More stable gene was observed in animals with desirable and intermediate genotypes to genes ESR/NCOA1/PRLR/FSHR. Key words: gene receptor gene, lymphocyte, sow, multiple. . ., . ., . . , , 62341, , . , e-mail: zoya_emez@mail.ru - , - , - , - , . - - - , - . - , - - . , , - . , , , , , - [2]. - - , , , , - . - - ( , ).

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