Морфологічні та ростові особливості нової лінії клітин людини 4BL
Morphology of cells and growth curve are important characteristics of cell line, so the aim of this research was to study these peculiarities. Methods. We investigated original cell line 4BL, obtained from peripheral blood of healthy donor, which was successfully passed through the Heyflick limit. M...
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| Published in: | Фактори експериментальної еволюції організмів |
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| Date: | 2013 |
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| Language: | Ukrainian |
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Інститут молекулярної біології і генетики НАН України
2013
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/177813 |
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| Cite this: | Морфологічні та ростові особливості нової лінії клітин людини 4BL / В.О. Кушнірук, Т.П. Рубан, Л.Л. Лукаш // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 315-319. — Бібліогр.: 6 назв. — укр. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1860114891024629760 |
|---|---|
| author | Кушнірук, В.О. Рубан, Т.П. Лукаш, Л.Л. |
| author_facet | Кушнірук, В.О. Рубан, Т.П. Лукаш, Л.Л. |
| citation_txt | Морфологічні та ростові особливості нової лінії клітин людини 4BL / В.О. Кушнірук, Т.П. Рубан, Л.Л. Лукаш // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 315-319. — Бібліогр.: 6 назв. — укр. |
| collection | DSpace DC |
| container_title | Фактори експериментальної еволюції організмів |
| description | Morphology of cells and growth curve are important characteristics of cell line, so the aim of this research was to study these peculiarities. Methods. We investigated original cell line 4BL, obtained from peripheral blood of healthy donor, which was successfully passed through the Heyflick limit. Methods of cell cultivation and standard cytological methods were used. Results. Cell line 4BL and its clones consist of two main types of cells: fibroblast-like and epithelioid. Cells have non-random distribution on the surface of culture dish and form cycle-like structures. These properties indirectly denote about stem potential of these cells, what have been confirmed by special investigations. Growth curves had graded character: virtually absence of lag-phase, two periods of exponential growth with phase of growth impairment between them, virtually absence of stationary phase and two peaks with maximum quantity of cells and accordingly two-step decreasing. Conclusions. Fibrgoblast-like and epithelioid cells are two main morphological types of cell line 4BL, so as it’s clones 1, 2 and 3. Ability to form cycle structures at the surface of culture dish was observed. Growth curves had graded character.

Key words: human cell line, growth curves, stem cells.
|
| first_indexed | 2025-12-07T17:36:14Z |
| format | Article |
| fulltext |
315
KOMISSAROVA S.M. 1, CHAKOVA N.N. 2, KRUPNOVA E.V. 2, MICHALENKO .P. 2,
CHEBOTARYOVA N.V. 2, NIYAZOVA S.S. 2
1 The Republican and Practical Centre «Cardiology»
2 Genetics and Cytology Institute of the National Academy of Sciences of Belarus, Minsk
Belarus, 220036, Minsk, R. Luxemburg str., 110, e-mail: kom_svet@mail.ru
INDIVIDUALIZATION OF ANGIOTENSIN II BETA-BLOCKER TREATMENT IN PATINETS
WITH HYPERTROPHIC CARDIOMYOPATHY
Aims. To assess the influence of gene polymorphism, coding RAAS proteins (I/D gene polymorphism ACE
and 1188 gene polymorphism AGTR1) on the effectiveness and safety of ARB therapy (losartan) in pa-
tients with HCMP. Methods. The study comprised 73 patients with HCMP (54 males and 19 females, mean-
age 46,7±15,7 yrs.). All patients received losartan during 12 months. All patients showed I/D- polymor-
phism in gene and change in 1166 of gene AGTR1 using PCR-RFLP analysis. Results. Losartan
therapy improved hemodynamic state in HCMP patients, but was unevenly effective in respect of left ven-
tricular hypertrophy regression (LVH). The greatest positive effect of treatment was noted in most of the pa-
tients (87,5 %) that were the carriers of heterozygous genotype combination (AGTR1)/ID(ACE) and in
58,3 % of carriers of homozygous genotypes (AGTR1)/DD(ACE). In subjects with genotype combina-
tion of AA(AGTR1)/II ACE a resistant LVH was discovered in 87,5 % of cases. Conclusion. Prior to
losartan therapy, it is reasonable to perform genotyping of RAAS proteins (I/D gene polymorphism ACE and
1188 gene polymorphism AGTR1), which allows considering genetic factors susceptible to the drug ad-
ministered.
Key words: hypertrophic cardiomyopathy, AGTR1 and ACE gene polymorphism, angiotensin II beta-
blocker.
. ., . ., . .
, , 03143, . . , 150, e-mail: kushniruk_v_o@ukr.net
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1. Sng J., Lufkin T. Emerging Stem Cell Therapies: Treatment, Safety, and Biology // Stem Cells International. –
2012. – P. 1–9.
2. Jacobs S.A., Roobrouck V.D., Verfaillie C.M., Gool S.W.V. Immunological characteristics of human mesenchymal
319
stem cells and multipotent adult progenitor cells // Immunol. Cell Biol. – 2013. – Vol. 91, 1. – . 32–39.
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6. Morange M. The scientific legacy of Jacques Monod // Research in Microbiology. – 2010. – Vol. 161. – . 77–81
p.
KUSHNIRUK V.O., RUBAN T.P., LUKASH L.L.
Institute of Molecular Biology and Genetics of NAS of Ukraine
Ukraine, 03680, Kyiv, Zabolotnogo str., 150, e-mail: kushniruk_v_o@ukr.net
MORPHOLOGICAL AND GROWTH PECULIARITIES OF NEW HUMAN CELL LINE 4BL
Morphology of cells and growth curve are important characteristics of cell line, so the aim of this research
was to study these peculiarities. Methods. We investigated original cell line 4BL, obtained from peripheral
blood of healthy donor, which was successfully passed through the Heyflick limit. Methods of cell cultiva-
tion and standard cytological methods were used. Results. Cell line 4BL and its clones consist of two main
types of cells: fibroblast-like and epithelioid. Cells have non-random distribution on the surface of culture
dish and form cycle-like structures. These properties indirectly denote about stem potential of these cells,
what have been confirmed by special investigations. Growth curves had graded character: virtually absence
of lag-phase, two periods of exponential growth with phase of growth impairment between them, virtually
absence of stationary phase and two peaks with maximum quantity of cells and accordingly two-step de-
creasing. Conclusions. Fibrgoblast-like and epithelioid cells are two main morphological types of cell line
4BL, so as it’s clones 1, 2 and 3. Ability to form cycle structures at the surface of culture dish was observed.
Growth curves had graded character.
Key words: human cell line, growth curves, stem cells.
. ., . .
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|
| id | nasplib_isofts_kiev_ua-123456789-177813 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 2219-3782 |
| language | Ukrainian |
| last_indexed | 2025-12-07T17:36:14Z |
| publishDate | 2013 |
| publisher | Інститут молекулярної біології і генетики НАН України |
| record_format | dspace |
| spelling | Кушнірук, В.О. Рубан, Т.П. Лукаш, Л.Л. 2021-02-16T19:11:50Z 2021-02-16T19:11:50Z 2013 Морфологічні та ростові особливості нової лінії клітин людини 4BL / В.О. Кушнірук, Т.П. Рубан, Л.Л. Лукаш // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 315-319. — Бібліогр.: 6 назв. — укр. 2219-3782 https://nasplib.isofts.kiev.ua/handle/123456789/177813 Morphology of cells and growth curve are important characteristics of cell line, so the aim of this research was to study these peculiarities. Methods. We investigated original cell line 4BL, obtained from peripheral blood of healthy donor, which was successfully passed through the Heyflick limit. Methods of cell cultivation and standard cytological methods were used. Results. Cell line 4BL and its clones consist of two main types of cells: fibroblast-like and epithelioid. Cells have non-random distribution on the surface of culture dish and form cycle-like structures. These properties indirectly denote about stem potential of these cells, what have been confirmed by special investigations. Growth curves had graded character: virtually absence of lag-phase, two periods of exponential growth with phase of growth impairment between them, virtually absence of stationary phase and two peaks with maximum quantity of cells and accordingly two-step decreasing. Conclusions. Fibrgoblast-like and epithelioid cells are two main morphological types of cell line 4BL, so as it’s clones 1, 2 and 3. Ability to form cycle structures at the surface of culture dish was observed. Growth curves had graded character.
 
 Key words: human cell line, growth curves, stem cells. uk Інститут молекулярної біології і генетики НАН України Фактори експериментальної еволюції організмів Генетика людини та медична генетика Морфологічні та ростові особливості нової лінії клітин людини 4BL Morphological and growth peculiarities of new human cell line 4BL Article published earlier |
| spellingShingle | Морфологічні та ростові особливості нової лінії клітин людини 4BL Кушнірук, В.О. Рубан, Т.П. Лукаш, Л.Л. Генетика людини та медична генетика |
| title | Морфологічні та ростові особливості нової лінії клітин людини 4BL |
| title_alt | Morphological and growth peculiarities of new human cell line 4BL |
| title_full | Морфологічні та ростові особливості нової лінії клітин людини 4BL |
| title_fullStr | Морфологічні та ростові особливості нової лінії клітин людини 4BL |
| title_full_unstemmed | Морфологічні та ростові особливості нової лінії клітин людини 4BL |
| title_short | Морфологічні та ростові особливості нової лінії клітин людини 4BL |
| title_sort | морфологічні та ростові особливості нової лінії клітин людини 4bl |
| topic | Генетика людини та медична генетика |
| topic_facet | Генетика людини та медична генетика |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/177813 |
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