Значение однонуклеотидного полиморфизма +276G/Т гена адипонектина (ADIPOQ) в формировании риска cахарного диабета 2 типа
Aim. To examine the association of single nucleotide polymorphism (SNP) +276G/Т ADIPOQ gene with Type 2 diabetes mellitus in slavonic population of Kharkov – Ukrainians and Russians. Methods. SNР ADIPOQ was detected in 544 type 2 diabetic patients and 140 subjects without ischemic heart disease, art...
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| Опубліковано в: : | Фактори експериментальної еволюції організмів |
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| Дата: | 2013 |
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Інститут молекулярної біології і генетики НАН України
2013
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| Цитувати: | Значение однонуклеотидного полиморфизма +276G/Т гена адипонектина (ADIPOQ) в формировании риска cахарного диабета 2 типа / Л.А. Атраментова, М.Ю. Горшунская, Ю.И. Караченцев, Н.А. Кравчун, Т.В. Тыжненко, А.К. Почерняев, Ю.А. Опалейко, В.В. Полторак // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 280-283. — Бібліогр.: 19 назв. — рос. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1859773883570192384 |
|---|---|
| author | Атраментова, Л.А. Горшунская, М.Ю. Караченцев, Ю.И. Кравчун, Н.А. Тыжненко, Т.В. Почерняев, А.К. Опалейко, Ю.А. Полторак, В.В. |
| author_facet | Атраментова, Л.А. Горшунская, М.Ю. Караченцев, Ю.И. Кравчун, Н.А. Тыжненко, Т.В. Почерняев, А.К. Опалейко, Ю.А. Полторак, В.В. |
| citation_txt | Значение однонуклеотидного полиморфизма +276G/Т гена адипонектина (ADIPOQ) в формировании риска cахарного диабета 2 типа / Л.А. Атраментова, М.Ю. Горшунская, Ю.И. Караченцев, Н.А. Кравчун, Т.В. Тыжненко, А.К. Почерняев, Ю.А. Опалейко, В.В. Полторак // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 280-283. — Бібліогр.: 19 назв. — рос. |
| collection | DSpace DC |
| container_title | Фактори експериментальної еволюції організмів |
| description | Aim. To examine the association of single nucleotide polymorphism (SNP) +276G/Т ADIPOQ gene with Type 2 diabetes mellitus in slavonic population of Kharkov – Ukrainians and Russians. Methods. SNР ADIPOQ was detected in 544 type 2 diabetic patients and 140 subjects without ischemic heart disease, arterial hypertension and diabetes. Genotyping of the SNP was performed by using the polymerase chain reaction – restriction fragments length polymorphism method. The statistical analysis was made, genotype and allele frequencies were tested by χ2. The Odds Ratio (OR) was calculated and a 95 % confidence interval (CI) was provide. Results. The genotype frequencies were consistent with Hardy-Weinberg equilibrium in both groups. Frequency of G allele is 0.69 in control group, and 0.60 in patients. The TT genotype was present in 8.6 % of control subjects, and in 16.6 % of patients (p<0.05). The TT genotype was associated with increased risk for type 2 diabetes mellitus (OR=2.00; CI 1.05-3.78; p<0,05). Homozygosity for major allele was associated with decreased risk of disease (OR=0.66;CI0,45-0,96 p<0,05). Conclusion. The TT genotype is a factor of increased risk for type 2 diabetes mellitus in the slavonic population.
Key words: SNP 276 G/T adiponectine ADIPOQ, type 2 diabetes mellitus.
|
| first_indexed | 2025-12-02T07:48:10Z |
| format | Article |
| fulltext |
280
1. Myres NM, Rootsi S, Lin AA at al. (18 co-authors). A major Y-chromosome haplogroup R1b Holocene era foun-
der effect in Central and Western Europe // Eur. J. Hum. Genet. – 2011. – Vol. 19, 1. – P. 95–101.
2. Rootsi S, Myres NM, Lin A.A. (33 co-authors) Distinguishing the co-ancestries of haplogroup G Y-chromosomes
in the populations of Europe and the Caucasus // Eur. J. Hum. Genet. – 2012. – Vol. 20, 12. – P. 1275–1282.
3. Semino O, Magri C, Benuzzi G. at al. (16 co-authors) Origin, diffusion, and differentiation of Y-chromosome hap-
logroups E and J: inferences on the neolithization of Europe and later migratory events in the Mediterranean area //
Am. J. Hum. Genet. – 2004. – 74 (5). – P. 1023–1034.
4. Underhill P.A., Myres N.M., Rootsi S. (34 co-authors). Separating the post-Glacial coancestry of European and
Asian Y chromosomes within haplogroup R1a // Eur. J. Hum. Genet. – 2010. – 18 (4). P. 479–484.
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AGDZHOYAN A.T. 1, UTEVSKA . . 5, SKHALYAKHO R.A. 2, DIBIROVA KH.D. 2, 1,
POCHESHKHOVA E.A. 3, 2, YUSUPOV Y.M. 4, MANSUROV R.I. 2, NAUMOVA E.A. 6,
ATRAMENTOVA L.A. 5, BALANOVSKA E.V. 2, BALANOVSKY O.P. 1, 2
1 Vavilov Institute for General Genetics, Russian Academy of Sciences
Russia, 119991, Moscow, GSP-1, Gubkin 3, e-mail: aagdzhoyan@gmal.com
2 Research Centre of Medical Genetics of the Russian Academy of Medical Science
Russia, Moscow
3 Kuban State Medical University, Krasnodar, Russia
4 Institute for Humanities Research of the Republic of Bashkortostan, Ufa, Russia
5 V.N. Karazin Kharkiv National University, Kharkiv, Ukraine
6 Lomonosov Moscow State University Moscow, Russia
TRACES OF ANCIENT MIGRATIONS IN THE CRIMEAN AND KAZAN TATARS GENEPOOLS:
THE ANALYSIS OF Y-CHROMOSOME POLYMORPHISM
Aims. The comparative analysis of gene pools of the Crimean and Kazan Tatars by the Y chromosome
markers. Methods. The molecular, statistical and cartographical methods were used. Results. A high
heterogeneity and lack of a dominant haplogroup were found for the gene pool of Crimean Tatars.
Conclusions. The Crimean Tatars gene pool includes the «Middle Eastern», «Mediterranean» and, to a lesser
extent, «Asian» haplogroups, and for Kazan Tatars the «Finno-Ugric» haplogroups are typical. The gene
pools of the Crimean and Kazan Tatars are placed on the different «poles» of the genetic space of Eurasian
Turkic peoples.
Key words: Y-chromosome haplogroup, population, gene pool, Crimean and Kazan Tatars.
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(ADIPOQ) -
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247 [4], -
281
-
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–
.
544 -
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140 ,
, -
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. -
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.
Chelex-100 [15].
+276G/T (rs1501299),
-
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)
.
ADIPOQ,
+276G/ , (ADIPOQ276F
ggcctctttcatcacagacc) (ADIPOQ276R
agatgcagcaaagccaaagt) .
-
pUC19,
MspI. -
BsmI (Mva1269I) -
2 %
[16]. -
-
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196 . ., -
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)
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196 . .
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282
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OR (Odds Ratio) 95 %- -
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. -
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, -
,
-
2
.
G.
0,693, 0,601. ,
(0,399 0,307 ). -
,
,
2 -
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(pG=0,689).
- ,
(16,9 8,6 % -
), -
(37,1 47,1 %).
. 2
, n (%)
GG GT TT
pG
66 (47,1) 62 (44,3) 12 (8,6) 0,693
2 202 (37,1) 250 (46,0) 92 (16,9) 0,601
: n – , pG – G, 2 – 2 .
(
2 ) , -
(r=0,09; 2=5,3;
2(0,05)=3,8; <0,05). -
- -
r=0,07; 2=7,5; 2(0,01)=6,6; <0,01. -
-
2
(OR=2,00; 95 % 1,05-3,78; <0,05), -
-
(OR=0,66; 95 % 0,45–0,96; <0,05).
,
, , -
2 ,
,
17 %, 91 %. -
, -
-
7,6 %, -
G - -
-
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-
– [19].
,
-
, -
.
1. Lu Qi, Tricia Li, Eric Rim t. al. The +276 polymorphism of the APM1 gene, plasma adiponectin concentration,
and cardiovascular risk in diabetic men // Diabetes. – 2005. – Vol.54. – P. 1607–1610.
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all publicity available DNA sequences.
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B., Froguel P., Marre M. Adiponectin gene polymorphisms and adiponectin levels are independently associated
with the development of hyperglycemia during a 3-year period // Diabetes. – 2004. – Vol. 53. – P. 1150–1157.
6. Hu F.B., Doria A., Meigs J.D. et.al. Genetic variation of the adiponectin locus and risk of type 2 diabetes in women
// Diabetes. – 2004. – Vol. 53. – P. 209–231.
7. Ohashi K., Ouchi N., Kihara S., et. al. Adiponectin 1164T mutation is associated with the metabolic syndrome and
coronary artery disease // J. Am. Coll Cardiol. – 2004. – P. 1195-2000.
8. Tso A.W.K, Sham P.C., Wat N.M.S., Xu A., Cheung B.M.Y.,.Rong R, Fong C.H.Y., Xu J.Y., Cheng K.K.Y., Janus
E.D., Lam K.S.L. Polymorphism of the gene encoding adiponectin and outcome of Chinese subjects with impared
glucose tolerance: a 5-year follow-up study // Diabetologia. – 2006. – Vol. 49. – P. 1806–1815.
9. Vasseur F., Helbecque N., Dina C. et al. Single-nucleotide polymorphism haplotypes in both proximal promoter
and exon 3 f the APM1 gene modulate adipocyte-secreted adiponectin hormone levels and contribute to the genetic
risk for type 2 diabetes in French Caucasians // Hum Mol Genet. – 2002. – Vol.11. – P. 2607–2614.
10. Stumvoll M., Tschritter O., Fritsche A. t. al. Assotiation of the T-G polymorphism in adiponectin (exon 2) with
obesity and insulin sensitivity // Diabetes. – 2002. – Vol. 51. – P. 37–41.
11. Menzaghi C., Ercolo T., Paola RD et. al. A haplotype at the adiponectin locus is associated with obesity and other
features of the insulin resistance syndrome // Diabetes. – 2002. – Vol. 51. – P. 2306–2312.
12. Hara K., Boutin P., Mori Y. et al. Genetic variation in the gene encoding adiponectin is associated with an in-
creased risk of type 2 diabetes in the Japanese population // Diabetes. – 2002. – Vol. 51. – . 536–540.
13. Ukkola O, Ravussin E, Jacobson P, Sjostrom L, Bouchard C. Mutations in the adiponectin gene in lean and obese
subjects from the Swedish obese subjects cohort // Metabolism – 2003. – Vol. 52. – P. 881–884.
14. Vozarova de Courten B., Hansoon R.L., Funahashi T. et. al. Common polymorphisms in the adiponectin gene
ACDC are not associated with diabetes in Pima Indians // Diabetes. – 2005. – Vol. 54. – P. 284–289.
15. Walsh P.S., Metzger D.A., Higuchi R. Chelex 100 as a medium for extraction of DNA for PCR-based typing from
forensic material // BioTechniques. – 1991.– 10. – P. 506–513.
16. Reddy, M.N. association of adiponectin gene functional polymorphisms (+45 /G and +276 G/ ) with obese
Breast Cancer / M.N. Reddy, K. Kumar, K. Jamil // J. Mol. Biomark. Diagn. – 2012. – Vol. 3. – P. 1–6.
17. Armitage P., Berry G. Statistical Methods in Medical Research // 3rd ed. Blackwell Scientific Publications. – Lon-
don, 1994. – 620 p.
18. 2010 . . – 2011. – 32 .
19. – / . . . . –
.: - - , 2009. – 528 .
RAMENTOVA L. . 1, GORSHUNSKAYA .Y. 2, KARACHENTSEV Y.I. 1, RAVCHUN N. . 1,
YZHNENKO .V. 1, P CHERNIAEV . . 1, P L I J. . 1, POLTORAK V.V. 1
1 V. Danilevsky Institute of Endocrine pathology problems at NAMS of Ukraine
Ukraine, 61002, Kharkov , Artema str., 10
2 Kharkiv Postgraduate Medical Academy
Ukraine, 61176, Kharkov, Korchagintsev str., 58, e-mail: atramentova@yandex.ru
ROLE OF SINGLE NUCLEOTIDE POLYMORPHISM +276G/ OF ADIPONECTIN GENE
(ADIPOQ) IN RISK OF TYPE 2 DIABETES MELLITUS DEVELOPMENT
Aim. To examine the association of single nucleotide polymorphism (SNP) +276G/ ADIPOQ gene with
Type 2 diabetes mellitus in slavonic population of Kharkov – Ukrainians and Russians. Methods. SN ADI-
POQ was detected in 544 type 2 diabetic patients and 140 subjects without ischemic heart disease, arterial
hypertension and diabetes. Genotyping of the SNP was performed by using the polymerase chain reaction –
restriction fragments length polymorphism method. The statistical analysis was made, genotype and allele
frequencies were tested by 2. The Odds Ratio (OR) was calculated and a 95 % confidence interval (CI) was
provide. Results. The genotype frequencies were consistent with Hardy-Weinberg equilibrium in both
groups. Frequency of G allele is 0.69 in control group, and 0.60 in patients. The TT genotype was present in
8.6 % of control subjects, and in 16.6 % of patients (p<0.05). The TT genotype was associated with
increased risk for type 2 diabetes mellitus (OR=2.00; CI 1.05-3.78; p<0,05). Homozygosity for major allele
was associated with decreased risk of disease (OR=0.66; CI 0,45-0,96 p<0,05). Conclusion. The TT genotype
is a factor of increased risk for type 2 diabetes mellitus in the slavonic population.
Key words: SNP 276 G/T adiponectine ADIPOQ, type 2 diabetes mellitus.
|
| id | nasplib_isofts_kiev_ua-123456789-177821 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 2219-3782 |
| language | Russian |
| last_indexed | 2025-12-02T07:48:10Z |
| publishDate | 2013 |
| publisher | Інститут молекулярної біології і генетики НАН України |
| record_format | dspace |
| spelling | Атраментова, Л.А. Горшунская, М.Ю. Караченцев, Ю.И. Кравчун, Н.А. Тыжненко, Т.В. Почерняев, А.К. Опалейко, Ю.А. Полторак, В.В. 2021-02-16T19:14:24Z 2021-02-16T19:14:24Z 2013 Значение однонуклеотидного полиморфизма +276G/Т гена адипонектина (ADIPOQ) в формировании риска cахарного диабета 2 типа / Л.А. Атраментова, М.Ю. Горшунская, Ю.И. Караченцев, Н.А. Кравчун, Т.В. Тыжненко, А.К. Почерняев, Ю.А. Опалейко, В.В. Полторак // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 280-283. — Бібліогр.: 19 назв. — рос. 2219-3782 https://nasplib.isofts.kiev.ua/handle/123456789/177821 Aim. To examine the association of single nucleotide polymorphism (SNP) +276G/Т ADIPOQ gene with Type 2 diabetes mellitus in slavonic population of Kharkov – Ukrainians and Russians. Methods. SNР ADIPOQ was detected in 544 type 2 diabetic patients and 140 subjects without ischemic heart disease, arterial hypertension and diabetes. Genotyping of the SNP was performed by using the polymerase chain reaction – restriction fragments length polymorphism method. The statistical analysis was made, genotype and allele frequencies were tested by χ2. The Odds Ratio (OR) was calculated and a 95 % confidence interval (CI) was provide. Results. The genotype frequencies were consistent with Hardy-Weinberg equilibrium in both groups. Frequency of G allele is 0.69 in control group, and 0.60 in patients. The TT genotype was present in 8.6 % of control subjects, and in 16.6 % of patients (p<0.05). The TT genotype was associated with increased risk for type 2 diabetes mellitus (OR=2.00; CI 1.05-3.78; p<0,05). Homozygosity for major allele was associated with decreased risk of disease (OR=0.66;CI0,45-0,96 p<0,05). Conclusion. The TT genotype is a factor of increased risk for type 2 diabetes mellitus in the slavonic population. Key words: SNP 276 G/T adiponectine ADIPOQ, type 2 diabetes mellitus. ru Інститут молекулярної біології і генетики НАН України Фактори експериментальної еволюції організмів Генетика людини та медична генетика Значение однонуклеотидного полиморфизма +276G/Т гена адипонектина (ADIPOQ) в формировании риска cахарного диабета 2 типа Role of single nucleotide polymorphism +276G/Т of adiponectin gene (ADIPOQ) in risk of type 2 diabetes mellitus development Article published earlier |
| spellingShingle | Значение однонуклеотидного полиморфизма +276G/Т гена адипонектина (ADIPOQ) в формировании риска cахарного диабета 2 типа Атраментова, Л.А. Горшунская, М.Ю. Караченцев, Ю.И. Кравчун, Н.А. Тыжненко, Т.В. Почерняев, А.К. Опалейко, Ю.А. Полторак, В.В. Генетика людини та медична генетика |
| title | Значение однонуклеотидного полиморфизма +276G/Т гена адипонектина (ADIPOQ) в формировании риска cахарного диабета 2 типа |
| title_alt | Role of single nucleotide polymorphism +276G/Т of adiponectin gene (ADIPOQ) in risk of type 2 diabetes mellitus development |
| title_full | Значение однонуклеотидного полиморфизма +276G/Т гена адипонектина (ADIPOQ) в формировании риска cахарного диабета 2 типа |
| title_fullStr | Значение однонуклеотидного полиморфизма +276G/Т гена адипонектина (ADIPOQ) в формировании риска cахарного диабета 2 типа |
| title_full_unstemmed | Значение однонуклеотидного полиморфизма +276G/Т гена адипонектина (ADIPOQ) в формировании риска cахарного диабета 2 типа |
| title_short | Значение однонуклеотидного полиморфизма +276G/Т гена адипонектина (ADIPOQ) в формировании риска cахарного диабета 2 типа |
| title_sort | значение однонуклеотидного полиморфизма +276g/т гена адипонектина (adipoq) в формировании риска cахарного диабета 2 типа |
| topic | Генетика людини та медична генетика |
| topic_facet | Генетика людини та медична генетика |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/177821 |
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