Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши
Aims. The aim of this study was a comparative analysis of the effects of 1,1-dimethyl hydrazine (1,1-DMH) – a component of rocket fuel, and anti-cancer drug cyclophosphan (CP) to the structure of the synaptonemal complex (SC) of male mice and selection of the defective spermatocytes I at meiosis. Me...
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| Cite this: | Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши / М.М. Ацаева, О.Л. Коломиец // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 284-286. — Бібліогр.: 11 назв. — рос. |
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Ацаева, М.М. Коломиец, О.Л. 2021-02-16T19:14:38Z 2021-02-16T19:14:38Z 2013 Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши / М.М. Ацаева, О.Л. Коломиец // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 284-286. — Бібліогр.: 11 назв. — рос. 2219-3782 https://nasplib.isofts.kiev.ua/handle/123456789/177822 Aims. The aim of this study was a comparative analysis of the effects of 1,1-dimethyl hydrazine (1,1-DMH) – a component of rocket fuel, and anti-cancer drug cyclophosphan (CP) to the structure of the synaptonemal complex (SC) of male mice and selection of the defective spermatocytes I at meiosis. Methods. Immuno-fluorescent analysis of the structure of SC damage and chromatin silencing. Results. Multiple damage in the structure of the SCs or even the absence of SC has been detected at the spermatocytes nuclei after the introduction of 1,1-DMH and CP tо male mice. The most common damage were fragmentation of SCs, the disturbance of the chromosome synapsis and architectonics of the nuclei. Ring chromosome and SCP3 protein aggregates were also found. There were every indication of pachytene arrest at many nuclei. We revealed morphological features, the so-called meiotic catastrophe that blocked meiosis at the different stages of meiosis. Conclusions. 1,1-DMH and CP cause dramatic damage of meiotic chromosomes, which carries a risk of disruption of spermatogenesis, infertility and risk of chromosomal aberrations of offspring, as evidenced by the detection of abnormal sperm. Key words: synaptonemal complex, spermatocytes, mice, 1,1-dimethyl hydrazine, immunofluorescent analysis. ru Інститут молекулярної біології і генетики НАН України Фактори експериментальної еволюції організмів Генетика людини та медична генетика Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши Immunofluorescent analysis of the structure of synaptonemal complex damage induced by xenobiotics in mice spermatocytes Article published earlier |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| title |
Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши |
| spellingShingle |
Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши Ацаева, М.М. Коломиец, О.Л. Генетика людини та медична генетика |
| title_short |
Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши |
| title_full |
Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши |
| title_fullStr |
Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши |
| title_full_unstemmed |
Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши |
| title_sort |
иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши |
| author |
Ацаева, М.М. Коломиец, О.Л. |
| author_facet |
Ацаева, М.М. Коломиец, О.Л. |
| topic |
Генетика людини та медична генетика |
| topic_facet |
Генетика людини та медична генетика |
| publishDate |
2013 |
| language |
Russian |
| container_title |
Фактори експериментальної еволюції організмів |
| publisher |
Інститут молекулярної біології і генетики НАН України |
| format |
Article |
| title_alt |
Immunofluorescent analysis of the structure of synaptonemal complex damage induced by xenobiotics in mice spermatocytes |
| description |
Aims. The aim of this study was a comparative analysis of the effects of 1,1-dimethyl hydrazine (1,1-DMH) – a component of rocket fuel, and anti-cancer drug cyclophosphan (CP) to the structure of the synaptonemal complex (SC) of male mice and selection of the defective spermatocytes I at meiosis. Methods. Immuno-fluorescent analysis of the structure of SC damage and chromatin silencing. Results. Multiple damage in the structure of the SCs or even the absence of SC has been detected at the spermatocytes nuclei after the introduction of 1,1-DMH and CP tо male mice. The most common damage were fragmentation of SCs, the disturbance of the chromosome synapsis and architectonics of the nuclei. Ring chromosome and SCP3 protein aggregates were also found. There were every indication of pachytene arrest at many nuclei. We revealed morphological features, the so-called meiotic catastrophe that blocked meiosis at the different stages of meiosis. Conclusions. 1,1-DMH and CP cause dramatic damage of meiotic chromosomes, which carries a risk of disruption of spermatogenesis, infertility and risk of chromosomal aberrations of offspring, as evidenced by the detection of abnormal sperm.
Key words: synaptonemal complex, spermatocytes, mice, 1,1-dimethyl hydrazine, immunofluorescent analysis.
|
| issn |
2219-3782 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/177822 |
| citation_txt |
Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши / М.М. Ацаева, О.Л. Коломиец // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 284-286. — Бібліогр.: 11 назв. — рос. |
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AT acaevamm immunofluorescentnyianaliznarušeniivstrukturesinaptonemnyhkompleksovvyzvannyhksenobiotikamivspermatocitahmyši AT kolomiecol immunofluorescentnyianaliznarušeniivstrukturesinaptonemnyhkompleksovvyzvannyhksenobiotikamivspermatocitahmyši AT acaevamm immunofluorescentanalysisofthestructureofsynaptonemalcomplexdamageinducedbyxenobioticsinmicespermatocytes AT kolomiecol immunofluorescentanalysisofthestructureofsynaptonemalcomplexdamageinducedbyxenobioticsinmicespermatocytes |
| first_indexed |
2025-11-25T23:30:36Z |
| last_indexed |
2025-11-25T23:30:36Z |
| _version_ |
1850581870990852096 |
| fulltext |
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6. Allen J.W., Gibson J.B., Poorman P. ., Backer L.C., Moses M.J. Synaptonemal Complex Damage Induced by
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Vol. 201. – P. 313–324.
7. Counce S.J., Meyer G.F. Differentiation of the synaptonemal complex and the kinetochore in Locusta spermato-
cytes studied by whole mount electron microscopy // Chromosoma. – 1973. –Vol. 44. – P. 231–253.
8. Homolka D., Ivanek R., Capkova J., Jansa P., Forejt J. Chromosomal rearrangement interferes with meiotic X
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6. – P. 71–82.
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ATSAEVA M.M., KOLOMIETS O.L.
N.I. Vavilov Institute of General Genetics, RAS
Russia, 119991, Moscow, Gubkin str., 3, e-mail: olkolomiets@mail.ru
IMMUNOFLUORESCENT ANALYSIS OF THE STRUCTURE OF SYNAPTONEMAL
COMPLEX DAMAGE INDUCED BY XENOBIOTICS IN MICE SPERMATOCYTES
Aims. The aim of this study was a comparative analysis of the effects of 1,1-dimethyl hydrazine (1,1-DMH)
– a component of rocket fuel, and anti-cancer drug cyclophosphan (CP) to the structure of the synaptonemal
complex (SC) of male mice and selection of the defective spermatocytes I at meiosis. Methods. Immuno-
fluorescent analysis of the structure of SC damage and chromatin silencing. Results. Multiple damage in the
structure of the SCs or even the absence of SC has been detected at the spermatocytes nuclei after the intro-
duction of 1,1-DMH and CP t male mice. The most common damage were fragmentation of SCs, the dis-
turbance of the chromosome synapsis and architectonics of the nuclei. Ring chromosome and SCP3 protein
aggregates were also found. There were every indication of pachytene arrest at many nuclei. We revealed
morphological features, the so-called meiotic catastrophe that blocked meiosis at the different stages of mei-
osis. Conclusions. 1,1-DMH and CP cause dramatic damage of meiotic chromosomes, which carries a risk
of disruption of spermatogenesis, infertility and risk of chromosomal aberrations of offspring, as evidenced
by the detection of abnormal sperm.
Key words: synaptonemal complex, spermatocytes, mice, 1,1-dimethyl hydrazine, immunofluorescent analy-
sis.
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