Инкапсулированные генетически модифицированные клетки СНО-К1 как источник рекомбинантного FGF2 человека

• Date: 2013
• Main Authors: Рымарь, С.Е., Рачкевич, Н.О., Кулачко, А.В., Рубан, Т.А., Кордюм, В.А.
• Format: Article
• Language: Russian
• Published: Інститут молекулярної біології і генетики НАН України 2013
• Series: Фактори експериментальної еволюції організмів
• Subjects: Генетика людини та медична генетика
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/177999
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Инкапсулированные генетически модифицированные клетки СНО-К1 как источник рекомбинантного FGF2 человека / С.Е. Рымарь, Н.О. Рачкевич, А.В. Кулачко, Т.А. Рубан, В.А. Кордюм // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 326-330. — Бібліогр.: 12 назв. — рос.

The aim of the investigation was to obtain the transgenic mammalian cell line CHO-K1 that expresses recombinant human FGF2 and secretes it into cultural medium and to compare the abilities of a monolyer cell culture and the cells encapsulated in alginate microcapsules to produce and to secrete this protein. Methods. The non-viral gene transfer method, RT-PCR, Western blot analysis were used for the investigation. Results. The expression vector pC1-F contained the recombinant human FGF2 had been constructed. This vector was used for the CHO-K1 cells transfection. As a result, a stable transgenic cell line expressing FGF2 was obtained. A few positive signals were detected via Western blot analysis of the conditioned cultural media. The same protein products were revealed in a case of the conditioned cultural media where alginate microcapsules with the transgenic cells had been cultivated. Conclusion. Thus the obtained genetically modified CHO-K1 cells remain a sourсe of the recombinant human FGF2 after their encapsulation in alginate microcapsules. Key words: recombinant human FGF2, transfection, encapsulation, alginate microcapsule.