Инкапсулированные генетически модифицированные клетки СНО-К1 как источник рекомбинантного FGF2 человека
The aim of the investigation was to obtain the transgenic mammalian cell line CHO-K1 that expresses recombinant human FGF2 and secretes it into cultural medium and to compare the abilities of a monolyer cell culture and the cells encapsulated in alginate microcapsules to produce and to secrete this...
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Рымарь, С.Е. Рачкевич, Н.О. Кулачко, А.В. Рубан, Т.А. Кордюм, В.А. 2021-02-17T15:44:04Z 2021-02-17T15:44:04Z 2013 Инкапсулированные генетически модифицированные клетки СНО-К1 как источник рекомбинантного FGF2 человека / С.Е. Рымарь, Н.О. Рачкевич, А.В. Кулачко, Т.А. Рубан, В.А. Кордюм // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 326-330. — Бібліогр.: 12 назв. — рос. 2219-3782 https://nasplib.isofts.kiev.ua/handle/123456789/177999 The aim of the investigation was to obtain the transgenic mammalian cell line CHO-K1 that expresses recombinant human FGF2 and secretes it into cultural medium and to compare the abilities of a monolyer cell culture and the cells encapsulated in alginate microcapsules to produce and to secrete this protein. Methods. The non-viral gene transfer method, RT-PCR, Western blot analysis were used for the investigation. Results. The expression vector pC1-F contained the recombinant human FGF2 had been constructed. This vector was used for the CHO-K1 cells transfection. As a result, a stable transgenic cell line expressing FGF2 was obtained. A few positive signals were detected via Western blot analysis of the conditioned cultural media. The same protein products were revealed in a case of the conditioned cultural media where alginate microcapsules with the transgenic cells had been cultivated. Conclusion. Thus the obtained genetically modified CHO-K1 cells remain a sourсe of the recombinant human FGF2 after their encapsulation in alginate microcapsules. Key words: recombinant human FGF2, transfection, encapsulation, alginate microcapsule. ru Інститут молекулярної біології і генетики НАН України Фактори експериментальної еволюції організмів Генетика людини та медична генетика Инкапсулированные генетически модифицированные клетки СНО-К1 как источник рекомбинантного FGF2 человека Encapsulated genetically modified cells of CHO-K1 as source of human recombinant FGF2 Article published earlier |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| title |
Инкапсулированные генетически модифицированные клетки СНО-К1 как источник рекомбинантного FGF2 человека |
| spellingShingle |
Инкапсулированные генетически модифицированные клетки СНО-К1 как источник рекомбинантного FGF2 человека Рымарь, С.Е. Рачкевич, Н.О. Кулачко, А.В. Рубан, Т.А. Кордюм, В.А. Генетика людини та медична генетика |
| title_short |
Инкапсулированные генетически модифицированные клетки СНО-К1 как источник рекомбинантного FGF2 человека |
| title_full |
Инкапсулированные генетически модифицированные клетки СНО-К1 как источник рекомбинантного FGF2 человека |
| title_fullStr |
Инкапсулированные генетически модифицированные клетки СНО-К1 как источник рекомбинантного FGF2 человека |
| title_full_unstemmed |
Инкапсулированные генетически модифицированные клетки СНО-К1 как источник рекомбинантного FGF2 человека |
| title_sort |
инкапсулированные генетически модифицированные клетки сно-к1 как источник рекомбинантного fgf2 человека |
| author |
Рымарь, С.Е. Рачкевич, Н.О. Кулачко, А.В. Рубан, Т.А. Кордюм, В.А. |
| author_facet |
Рымарь, С.Е. Рачкевич, Н.О. Кулачко, А.В. Рубан, Т.А. Кордюм, В.А. |
| topic |
Генетика людини та медична генетика |
| topic_facet |
Генетика людини та медична генетика |
| publishDate |
2013 |
| language |
Russian |
| container_title |
Фактори експериментальної еволюції організмів |
| publisher |
Інститут молекулярної біології і генетики НАН України |
| format |
Article |
| title_alt |
Encapsulated genetically modified cells of CHO-K1 as source of human recombinant FGF2 |
| description |
The aim of the investigation was to obtain the transgenic mammalian cell line CHO-K1 that expresses recombinant human FGF2 and secretes it into cultural medium and to compare the abilities of a monolyer cell culture and the cells encapsulated in alginate microcapsules to produce and to secrete this protein. Methods. The non-viral gene transfer method, RT-PCR, Western blot analysis were used for the investigation. Results. The expression vector pC1-F contained the recombinant human FGF2 had been constructed. This vector was used for the CHO-K1 cells transfection. As a result, a stable transgenic cell line expressing FGF2 was obtained. A few positive signals were detected via Western blot analysis of the conditioned cultural media. The same protein products were revealed in a case of the conditioned cultural media where alginate microcapsules with the transgenic cells had been cultivated. Conclusion. Thus the obtained genetically modified CHO-K1 cells remain a sourсe of the recombinant human FGF2 after their encapsulation in alginate microcapsules.
Key words: recombinant human FGF2, transfection, encapsulation, alginate microcapsule.
|
| issn |
2219-3782 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/177999 |
| citation_txt |
Инкапсулированные генетически модифицированные клетки СНО-К1 как источник рекомбинантного FGF2 человека / С.Е. Рымарь, Н.О. Рачкевич, А.В. Кулачко, Т.А. Рубан, В.А. Кордюм // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 326-330. — Бібліогр.: 12 назв. — рос. |
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2025-11-25T22:45:17Z |
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2025-11-25T22:45:17Z |
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326
NEUMERZITSKAYA L.V., KLIMENKO S.W., KOVAL G.M., WERBILENKO R.M.,
V.N. SHKARUPA
Si «National S ientific Center for Radiation Medicine NAM S of Ukraine»
Ukraine, 04050, Kiev, Melnikov str., 53, e-mail: lneum@bigmir.net
STUDY OF CHROMOSOMAL ABNORMALITIES IN THE SOMATIC CELLS OF PATIENTS
WITH THYROID CANCER WHO SUFFERED FROM THE CHERNOBYL ACCIDENT
Aims. Research level and spectrum of chromosomal aberrations in peripheral blood lymphocytes of patients
with thyroid cancer, which were last ionizing radiation due to the Chernobyl accident. Methods. ytogenetic
analysis of human lymphocytes. Results. Cytogenetic studies of peripheral blood lymphocytes of patients
with thyroid cancer. This group of patients previously exposed to radiation. The frequency of chromosomal
aberrations in peripheral blood lymphocytes of patients with higher levels of spontaneous and significantly
higher than the control group.
Key words: thyroid cancer, chromosome aberrations, radiation.
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1. Krishnamurthy N.V., Gimi B. Encapsulated cell grafts to treat cellular deficiencies and dysfunction // Crit Rev.
Biomed. Eng. – 2011. – Vol. 39, 6. – P. 473–491.
2. Hernández R.M., Orive G., Murua A., Pedraz J.L. Microcapsules and microcarriers for in situ cell delivery //
Advanced Drug Delivery Reviews. – 2010. – Vol. 62. – P. 711–730.
3. Yun Y.R., Won J.E., Jeon E., Lee S., Kang W., Jo H., Jang J.H., Shin U.S., Kim H.W. Fibroblast growth factors:
biology, function, and application for tissue regeneration // J. Tissue Eng. – 2010. – P. 1–18.
4. Sambrook J., Fritsch E.F., Maniatis T. Molecular cloning // Cold Spring Harbor Lab. Press. – 1989. – Vol. 1. – P. 568.
5. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4 // Nature. –
1970. – Vol. 227, 5229. – P. 680–685.
6. Cohen J., Zaleski K.L., Nourissat G., Yaremchuk M.J. Survival of porcine mesenchymal stem cells over the
alginate recovered method // J. Biomed. Mater. Res. Part A. – 2010. – 96 A. – P. 93–99.
7. Zehe C, Engling A, Wegehingel S, Schäfer T, Nickel W. Cell-surface heparan sulfate proteoglycans are essential
components of the unconventional export machinery of FGF-2 // Proc. Natl. Acad. Sci. USA. – 2006. – Vol. 103. –
P. 15479–15484.
8. Soulet F., Bailly K., Roga S., Bouche G. Exogenously added FGF2 to NIH3T3 cells interacts with nuclear
ribosomal S6 kinase in cell cycle-dependent manner // JBC. – 2005. – Vol. 280. – P. 25604–25610.
9. Bonnet H., Filhol O., Truchet I., Bouche G. FGF2 binds to the regulatory subunit of CK2 and directly stimulates
CK2 activity toward nucleolin // JBC. – 1996. – Vol. 271. – P. 24781–24787.
10. Van den Berghe L., Laurell H., Bugler B. FIF, a nuclear putatively antiapoptotic factor, interacts specifically with
FGF2 // Mol. Endocrinol. – 2000. – Vol. 14. – P. 1709–1724.
11. Herr B., Ornitz D.M., Sasisekharan R., Venkataraman G., Waksman G. Heparin-induced Self-association of
Fibroblast Growth Factor-2 // JBC. – 1997. – Vol. 272. – P. 16382–16389.
12. Darrabie M.D., Kendall W.F.Jr., Opara E.C. Characteristics of Poly-L-Ornithine-coated alginate microcapsules //
Biomaterials. – 2005. – Vol. 26, 34. – P. 6846–6852.
100 m 50 m
330
RYMAR S.E., RACHKEVICH N.O., KULACHKO A.V., RUBAN T.A., KORDIUM V.A.
Institute of Molecular Biology and Genetics of NASU
Ukraine, 03680, Kyiv, Acad. Zabolotny str., 150, e-mail: s.y.rymar@imbg.org.ua
SI «Institute of Genetic and Regenarative Medicine» NASMU
Ukraine, 04114, Kyiv, Vyshgorodska str., 67
ENCAPSULATED GENETICALLY MODIFIED CELLS OF CHO-K1 AS SOUR E OF
HUMAN RECOMBINANT FGF2
The aim of the investigation was to obtain the transgenic mammalian cell line CHO-K1 that expresses
recombinant human FGF2 and secretes it into cultural medium and to compare the abilities of a monolyer
cell culture and the cells encapsulated in alginate microcapsules to produce and to secrete this protein.
Methods. The non-viral gene transfer method, RT-PCR, Western blot analysis were used for the
investigation. Results. The expression vector pC1-F contained the recombinant human FGF2 had been
constructed. This vector was used for the CHO-K1 cells transfection. As a result, a stable transgenic cell line
expressing FGF2 was obtained. A few positive signals were detected via Western blot analysis of the
conditioned cultural media. The same protein products were revealed in a case of the conditioned cultural
media where alginate microcapsules with the transgenic cells had been cultivated. Conclusion. Thus the
obtained genetically modified CHO-K1 cells remain a sour e of the recombinant human FGF2 after their
encapsulation in alginate microcapsules.
Key words: recombinant human FGF2, transfection, encapsulation, alginate microcapsule.
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