Влияние возраста листьев сахарной свеклы на ПЦР-профили ферментных генов
Aims. In the previous researches we showed that during a sprout growth process and a tissue differentiation there is a change of DNA structure of enzyme loci. These changes, however, do not concern primary structure of coding genes part. We were interested by a question, whether there is a change of...
Saved in:
| Published in: | Фактори експериментальної еволюції організмів |
|---|---|
| Date: | 2014 |
| Main Authors: | , , |
| Format: | Article |
| Language: | Russian |
| Published: |
Інститут молекулярної біології і генетики НАН України
2014
|
| Subjects: | |
| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/178053 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Влияние возраста листьев сахарной свеклы на ПЦР-профили ферментных генов / Н.А. Виниченко, С.С. Кирикович, Е.В. Левитес // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2014. — Т. 14. — С. 95-100. — Бібліогр.: 10 назв. — рос. |
Institution
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862716619973722112 |
|---|---|
| author | Виниченко, Н.А. Кирикович, С.С. Левитес, Е.В. |
| author_facet | Виниченко, Н.А. Кирикович, С.С. Левитес, Е.В. |
| citation_txt | Влияние возраста листьев сахарной свеклы на ПЦР-профили ферментных генов / Н.А. Виниченко, С.С. Кирикович, Е.В. Левитес // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2014. — Т. 14. — С. 95-100. — Бібліогр.: 10 назв. — рос. |
| collection | DSpace DC |
| container_title | Фактори експериментальної еволюції організмів |
| description | Aims. In the previous researches we showed that during a sprout growth process and a tissue differentiation there is a change of DNA structure of enzyme loci. These changes, however, do not concern primary structure of coding genes part. We were interested by a question, whether there is a change of DNA in adult plants. For this purpose we decided to use PCR-profiles of leaves of different age from one plant. Methods. For researches were taken two sugar beet agamospermous progenies. For receiving PCR-profiles we used the modified method of the ISSR analysis. The main peculiarity of this modification is those: together with a microsatellite primer for PCR we used the primer which was specific to the enzyme locus. DNA was extracted from the youngest, average and senior leaves. Results. PCR-profiles for Adh1, Me1, Gpi1, Got1 loci were received. A wide variety of PCR-profiles of Adh1 locus was revealed even in homozygous plants at all studied stages of growth. PCR-profiles of a Me1 locus did not show a wide variety, but showed gradual transition of one PCR-profile to another one during leaf growth. It was revealed complication of PCR-profiles of Gpi1 and Got1 loci during leaf development and growth. Conclusions. Obtained results point out that there are changes of DNA structure of enzyme loci the adult plant of sugar beet during development and growth of a leaf. At present there are no data which point that these changes connected with changes of the nucleotide sequences of studied genes.

Key words: PCR-profiles, sugar beet, enzyme loci, ISSR analysis.
|
| first_indexed | 2025-12-07T18:06:16Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-178053 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 2219-3782 |
| language | Russian |
| last_indexed | 2025-12-07T18:06:16Z |
| publishDate | 2014 |
| publisher | Інститут молекулярної біології і генетики НАН України |
| record_format | dspace |
| spelling | Виниченко, Н.А. Кирикович, С.С. Левитес, Е.В. 2021-02-17T18:20:20Z 2021-02-17T18:20:20Z 2014 Влияние возраста листьев сахарной свеклы на ПЦР-профили ферментных генов / Н.А. Виниченко, С.С. Кирикович, Е.В. Левитес // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2014. — Т. 14. — С. 95-100. — Бібліогр.: 10 назв. — рос. 2219-3782 https://nasplib.isofts.kiev.ua/handle/123456789/178053 575.1:581.163:577.151.64:633.413:577.213 Aims. In the previous researches we showed that during a sprout growth process and a tissue differentiation there is a change of DNA structure of enzyme loci. These changes, however, do not concern primary structure of coding genes part. We were interested by a question, whether there is a change of DNA in adult plants. For this purpose we decided to use PCR-profiles of leaves of different age from one plant. Methods. For researches were taken two sugar beet agamospermous progenies. For receiving PCR-profiles we used the modified method of the ISSR analysis. The main peculiarity of this modification is those: together with a microsatellite primer for PCR we used the primer which was specific to the enzyme locus. DNA was extracted from the youngest, average and senior leaves. Results. PCR-profiles for Adh1, Me1, Gpi1, Got1 loci were received. A wide variety of PCR-profiles of Adh1 locus was revealed even in homozygous plants at all studied stages of growth. PCR-profiles of a Me1 locus did not show a wide variety, but showed gradual transition of one PCR-profile to another one during leaf growth. It was revealed complication of PCR-profiles of Gpi1 and Got1 loci during leaf development and growth. Conclusions. Obtained results point out that there are changes of DNA structure of enzyme loci the adult plant of sugar beet during development and growth of a leaf. At present there are no data which point that these changes connected with changes of the nucleotide sequences of studied genes.
 
 Key words: PCR-profiles, sugar beet, enzyme loci, ISSR analysis. ru Інститут молекулярної біології і генетики НАН України Фактори експериментальної еволюції організмів Епігенетика і генетика розвитку Влияние возраста листьев сахарной свеклы на ПЦР-профили ферментных генов Influence age sugar beet leaves on PCR profiles of enzyme genes Article published earlier |
| spellingShingle | Влияние возраста листьев сахарной свеклы на ПЦР-профили ферментных генов Виниченко, Н.А. Кирикович, С.С. Левитес, Е.В. Епігенетика і генетика розвитку |
| title | Влияние возраста листьев сахарной свеклы на ПЦР-профили ферментных генов |
| title_alt | Influence age sugar beet leaves on PCR profiles of enzyme genes |
| title_full | Влияние возраста листьев сахарной свеклы на ПЦР-профили ферментных генов |
| title_fullStr | Влияние возраста листьев сахарной свеклы на ПЦР-профили ферментных генов |
| title_full_unstemmed | Влияние возраста листьев сахарной свеклы на ПЦР-профили ферментных генов |
| title_short | Влияние возраста листьев сахарной свеклы на ПЦР-профили ферментных генов |
| title_sort | влияние возраста листьев сахарной свеклы на пцр-профили ферментных генов |
| topic | Епігенетика і генетика розвитку |
| topic_facet | Епігенетика і генетика розвитку |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/178053 |
| work_keys_str_mv | AT viničenkona vliânievozrastalistʹevsaharnoisveklynapcrprofilifermentnyhgenov AT kirikovičss vliânievozrastalistʹevsaharnoisveklynapcrprofilifermentnyhgenov AT levitesev vliânievozrastalistʹevsaharnoisveklynapcrprofilifermentnyhgenov AT viničenkona influenceagesugarbeetleavesonpcrprofilesofenzymegenes AT kirikovičss influenceagesugarbeetleavesonpcrprofilesofenzymegenes AT levitesev influenceagesugarbeetleavesonpcrprofilesofenzymegenes |