Определение Lr генов в сортах пшеницы мягкой озимой украинской селекции
Aims. The aim of our research was to identify Lr-genes in winter wheat Ukrainian varieties using DNA markers. Methods. DNA was isolated from a mixture of five seeds. Presence of Lr genes was studied by polymerase chain reaction (PCR). The amplification products were visualized by electrophoresis on...
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| Published in: | Фактори експериментальної еволюції організмів |
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| Date: | 2014 |
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| Language: | Russian |
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Інститут молекулярної біології і генетики НАН України
2014
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/178084 |
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| Cite this: | Определение Lr генов в сортах пшеницы мягкой озимой украинской селекции / Г.П. Зайцева, Г.Е. Акинина, Е.В. Твердохлеб, Ю.Н. Дугарь, В.Н. Попов // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2014. — Т. 14. — С. 150-153. — Бібліогр.: 11 назв. — рос. |
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Зайцева, Г.П. Акинина, Г.Е. Твердохлеб, Е.В. Дугарь, Ю.Н. Попов, В.Н. 2021-02-17T20:04:17Z 2021-02-17T20:04:17Z 2014 Определение Lr генов в сортах пшеницы мягкой озимой украинской селекции / Г.П. Зайцева, Г.Е. Акинина, Е.В. Твердохлеб, Ю.Н. Дугарь, В.Н. Попов // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2014. — Т. 14. — С. 150-153. — Бібліогр.: 11 назв. — рос. 2219-3782 https://nasplib.isofts.kiev.ua/handle/123456789/178084 633.11:575 Aims. The aim of our research was to identify Lr-genes in winter wheat Ukrainian varieties using DNA markers. Methods. DNA was isolated from a mixture of five seeds. Presence of Lr genes was studied by polymerase chain reaction (PCR). The amplification products were visualized by electrophoresis on a 2% agarose gel. For determination the numbers and sizes of the amplification products demo version of program TotalLab 120 was used. Results. Lr10 gene was detected in six varieties of winter wheat from two breeding centers – The Plant Production Institute (PPI) and The Plant Breeding and Genetics Institute (PBGI). In the studied varieties Lr19 and Lr20 genes were not identified. Lr34 gene was identified in 13 varieties of PPI, 17 varieties of PBGI and three varieties of the Mironivskiy institute of wheat (MIW). Conclusions. Using the DNA-markers varieties and lines of soft winter wheat with the Lr genes were identified. The two Lr genes, Lr10 and Lr34, are present in some studied samples. And two genes, Lr19 and Lr20, were not identified in studied Ukrainian winter wheat varieties and lines. Key words: Lr- genes, DNA markers, leaf rust, T. aestivum L. ru Інститут молекулярної біології і генетики НАН України Фактори експериментальної еволюції організмів Аналіз та оцінка генетичних ресурсів Определение Lr генов в сортах пшеницы мягкой озимой украинской селекции Determination of Lr genes in soft winter wheat varieties of ukrainian selection Article published earlier |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| title |
Определение Lr генов в сортах пшеницы мягкой озимой украинской селекции |
| spellingShingle |
Определение Lr генов в сортах пшеницы мягкой озимой украинской селекции Зайцева, Г.П. Акинина, Г.Е. Твердохлеб, Е.В. Дугарь, Ю.Н. Попов, В.Н. Аналіз та оцінка генетичних ресурсів |
| title_short |
Определение Lr генов в сортах пшеницы мягкой озимой украинской селекции |
| title_full |
Определение Lr генов в сортах пшеницы мягкой озимой украинской селекции |
| title_fullStr |
Определение Lr генов в сортах пшеницы мягкой озимой украинской селекции |
| title_full_unstemmed |
Определение Lr генов в сортах пшеницы мягкой озимой украинской селекции |
| title_sort |
определение lr генов в сортах пшеницы мягкой озимой украинской селекции |
| author |
Зайцева, Г.П. Акинина, Г.Е. Твердохлеб, Е.В. Дугарь, Ю.Н. Попов, В.Н. |
| author_facet |
Зайцева, Г.П. Акинина, Г.Е. Твердохлеб, Е.В. Дугарь, Ю.Н. Попов, В.Н. |
| topic |
Аналіз та оцінка генетичних ресурсів |
| topic_facet |
Аналіз та оцінка генетичних ресурсів |
| publishDate |
2014 |
| language |
Russian |
| container_title |
Фактори експериментальної еволюції організмів |
| publisher |
Інститут молекулярної біології і генетики НАН України |
| format |
Article |
| title_alt |
Determination of Lr genes in soft winter wheat varieties of ukrainian selection |
| description |
Aims. The aim of our research was to identify Lr-genes in winter wheat Ukrainian varieties using DNA markers. Methods. DNA was isolated from a mixture of five seeds. Presence of Lr genes was studied by polymerase chain reaction (PCR). The amplification products were visualized by electrophoresis on a 2% agarose gel. For determination the numbers and sizes of the amplification products demo version of program TotalLab 120 was used. Results. Lr10 gene was detected in six varieties of winter wheat from two breeding centers – The Plant Production Institute (PPI) and The Plant Breeding and Genetics Institute (PBGI). In the studied varieties Lr19 and Lr20 genes were not identified. Lr34 gene was identified in 13 varieties of PPI, 17 varieties of PBGI and three varieties of the Mironivskiy institute of wheat (MIW). Conclusions. Using the DNA-markers varieties and lines of soft winter wheat with the Lr genes were identified. The two Lr genes, Lr10 and Lr34, are present in some studied samples. And two genes, Lr19 and Lr20, were not identified in studied Ukrainian winter wheat varieties and lines.
Key words: Lr- genes, DNA markers, leaf rust, T. aestivum L.
|
| issn |
2219-3782 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/178084 |
| citation_txt |
Определение Lr генов в сортах пшеницы мягкой озимой украинской селекции / Г.П. Зайцева, Г.Е. Акинина, Е.В. Твердохлеб, Ю.Н. Дугарь, В.Н. Попов // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2014. — Т. 14. — С. 150-153. — Бібліогр.: 11 назв. — рос. |
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| first_indexed |
2025-12-07T17:40:34Z |
| last_indexed |
2025-12-07T17:40:34Z |
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