Клеточная экструзия ДНК лейкоцитами млекопитающих
Aims. The study of cellular extrusion of DNA of mammalian leukocytes. Methods. Leukocytes were isolated from human blood and mice in the capillaries by centrifugation. Human leukocytes and part white blood cells of mice placed onto a slide and fixed. Another portion was applied to mice leukocytes em...
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| Published in: | Фактори експериментальної еволюції організмів |
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| Date: | 2014 |
| Main Authors: | , , , , |
| Format: | Article |
| Language: | Russian |
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Інститут молекулярної біології і генетики НАН України
2014
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/178273 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Клеточная экструзия ДНК лейкоцитами млекопитающих / Л.И. Лихачева, С.П. Шпилевская, Т.А. Рубан, Т.П. Гулько, В.А. Кордюм // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2014. — Т. 15. — С. 91-95. — Бібліогр.: 18 назв. — рос. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862719957646704640 |
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| author | Лихачева, Л.И. Шпилевская, С.П. Рубан, Т.А. Гулько, Т.П. Кордюм, В.А. |
| author_facet | Лихачева, Л.И. Шпилевская, С.П. Рубан, Т.А. Гулько, Т.П. Кордюм, В.А. |
| citation_txt | Клеточная экструзия ДНК лейкоцитами млекопитающих / Л.И. Лихачева, С.П. Шпилевская, Т.А. Рубан, Т.П. Гулько, В.А. Кордюм // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2014. — Т. 15. — С. 91-95. — Бібліогр.: 18 назв. — рос. |
| collection | DSpace DC |
| container_title | Фактори експериментальної еволюції організмів |
| description | Aims. The study of cellular extrusion of DNA of mammalian leukocytes. Methods. Leukocytes were isolated from human blood and mice in the capillaries by centrifugation. Human leukocytes and part white blood cells of mice placed onto a slide and fixed. Another portion was applied to mice leukocytes embryonic fibroblasts, after 1.5–2 hour exposure preparations were fixed. The resulting specimens were stained with fluorescent dyes Hoechst 33342 DNA. or Syber-Green and analyzed by standard fluorescence microscopy. Results. Microscopy results showed that the emissions of DNA from leucocytes into the extracellular space as observed in their monoculture or in the presence of fibroblasts, while the extracellular DNA the nuclei of leukocytes is contacted with the fibroblasts. Conclusion. These results demonstrate that mammalian leukocytes may release DNA into the extracellular space as in monoculture and in co-culture with tissue culture cells. It is suggested that the phenomenon described here can serve as described here can serve as.

Key words: cellular extrusion of DNA, extracellular space.
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| first_indexed | 2025-12-07T18:23:02Z |
| format | Article |
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| id | nasplib_isofts_kiev_ua-123456789-178273 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 2219-3782 |
| language | Russian |
| last_indexed | 2025-12-07T18:23:02Z |
| publishDate | 2014 |
| publisher | Інститут молекулярної біології і генетики НАН України |
| record_format | dspace |
| spelling | Лихачева, Л.И. Шпилевская, С.П. Рубан, Т.А. Гулько, Т.П. Кордюм, В.А. 2021-02-18T12:29:42Z 2021-02-18T12:29:42Z 2014 Клеточная экструзия ДНК лейкоцитами млекопитающих / Л.И. Лихачева, С.П. Шпилевская, Т.А. Рубан, Т.П. Гулько, В.А. Кордюм // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2014. — Т. 15. — С. 91-95. — Бібліогр.: 18 назв. — рос. 2219-3782 https://nasplib.isofts.kiev.ua/handle/123456789/178273 577.1+576.5:591. 111.1 Aims. The study of cellular extrusion of DNA of mammalian leukocytes. Methods. Leukocytes were isolated from human blood and mice in the capillaries by centrifugation. Human leukocytes and part white blood cells of mice placed onto a slide and fixed. Another portion was applied to mice leukocytes embryonic fibroblasts, after 1.5–2 hour exposure preparations were fixed. The resulting specimens were stained with fluorescent dyes Hoechst 33342 DNA. or Syber-Green and analyzed by standard fluorescence microscopy. Results. Microscopy results showed that the emissions of DNA from leucocytes into the extracellular space as observed in their monoculture or in the presence of fibroblasts, while the extracellular DNA the nuclei of leukocytes is contacted with the fibroblasts. Conclusion. These results demonstrate that mammalian leukocytes may release DNA into the extracellular space as in monoculture and in co-culture with tissue culture cells. It is suggested that the phenomenon described here can serve as described here can serve as.
 
 Key words: cellular extrusion of DNA, extracellular space. ru Інститут молекулярної біології і генетики НАН України Фактори експериментальної еволюції організмів Клітинні, генні та молекулярні біотехнології Клеточная экструзия ДНК лейкоцитами млекопитающих Cellular extrusion of DNA by mammalian leukocytes Article published earlier |
| spellingShingle | Клеточная экструзия ДНК лейкоцитами млекопитающих Лихачева, Л.И. Шпилевская, С.П. Рубан, Т.А. Гулько, Т.П. Кордюм, В.А. Клітинні, генні та молекулярні біотехнології |
| title | Клеточная экструзия ДНК лейкоцитами млекопитающих |
| title_alt | Cellular extrusion of DNA by mammalian leukocytes |
| title_full | Клеточная экструзия ДНК лейкоцитами млекопитающих |
| title_fullStr | Клеточная экструзия ДНК лейкоцитами млекопитающих |
| title_full_unstemmed | Клеточная экструзия ДНК лейкоцитами млекопитающих |
| title_short | Клеточная экструзия ДНК лейкоцитами млекопитающих |
| title_sort | клеточная экструзия днк лейкоцитами млекопитающих |
| topic | Клітинні, генні та молекулярні біотехнології |
| topic_facet | Клітинні, генні та молекулярні біотехнології |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/178273 |
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