Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions
Aims. Molecular-genetic and cytological analyses were carried out to detect the alien genes in original introgression stocks and to investigate their inheritance. Methods. Rye (Xrems1303, SR1R003) and wheat (Xgwm18-1BS, Xgwm550-1BS, Xgwm140-1BL, Xgwm153-1BL, Xgwm357-1AL, Taglut-1AS) microsatellites...
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Інститут молекулярної біології і генетики НАН України
2014
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| Zitieren: | Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions / I.I. Motsny, L.V. Sudarchuk, A.V. Galaev, S.V. Chebotar // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2014. — Т. 15. — С. 264-268. — Бібліогр.: 14 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1860250379719016448 |
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| author | Motsny, I.I. Sudarchuk, L.V. Galaev, A.V. Chebotar, S.V. |
| author_facet | Motsny, I.I. Sudarchuk, L.V. Galaev, A.V. Chebotar, S.V. |
| citation_txt | Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions / I.I. Motsny, L.V. Sudarchuk, A.V. Galaev, S.V. Chebotar // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2014. — Т. 15. — С. 264-268. — Бібліогр.: 14 назв. — англ. |
| collection | DSpace DC |
| container_title | Фактори експериментальної еволюції організмів |
| description | Aims. Molecular-genetic and cytological analyses were carried out to detect the alien genes in original introgression stocks and to investigate their inheritance. Methods. Rye (Xrems1303, SR1R003) and wheat (Xgwm18-1BS, Xgwm550-1BS, Xgwm140-1BL, Xgwm153-1BL, Xgwm357-1AL, Taglut-1AS) microsatellites and secalin-specific STS-marker (ω-sec-P3+ ω-sec-P4) have been applied. Results. The (1B)1R wheat-rye chromosome substitution and 1BL.1RS translocation have been identified. The pairing between short arms of the 1BL.1RS translocation and of bread wheat chromosome 1B was observed with very low frequency (in 0.3 % PMCs). Conclusions. The stocks have (1B)1R wheat-rye chromosome substitution or 1BL.1RS translocation. The translocation was contributed by the collection strains, derived from wheat cv. Avrora and originated from Petkus rye. The intact rye chromosome 1R for the substitution was contributed by triticale (8x) cv. AD825 and originated from rye Voronezhskaya SHI. The substitution stocks were susceptible to leaf and stem rusts because of another origination of the 1R chromosome. Three major linked genes determining hairiness of the leaf upper surface (Hlup), lower surface (Hllow) and leaf margin (Hlm) were revealed. The genes were contributed by a synthetic (T. timopheevii/Ae. tauschii) and were non-allelic to Hl1 gene.

Key words: Triticum aestivum, (1B)1R substitution, 1BL.1RS translocation, hairy leaf, PCR-markers.
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264
metylhuanin DNA methyltransferase during establishment of cell lines in vitro // Biopol Cell. – 2013. – 29, N 6. –
Р. 485–492.
MACEWICZ L.L., LYLO V.V., KARPOVA I.S., KOTSARENKO K.V., RUBAN T.A., LUKASH L.L.
Institute of Molecular Biology and Genetics of Natl. Acad. Sci. of Ukraine,
Ukraine, 03680, Kyiv, Akad. Zabolotnogo str., 150, е-mail: lukash@imbg.org.ua
PLANT AND ANIMAL LECTINES AS MODULATORS OF MGMT AND MARP GENE
EXPRESSION IN VITRO
Aims. Previously for the first time we have studied the ability of lectins to influence the processes of
mutagenesis and antimutagenesis in different test systems. The aim of present study was to examine the
effect of panel of lectins on the MGMT and MARP expression levels in tumor and non-tumor mammalian
cells in vitro. Methods. Standard cell cultivation methods and Western blot analysis were used. Results. The
influence of plant and animal lectins (perk egg lectin, lentil seeds lectin and elderberry bark lectin) on
expressiom of proteins recognized by anti-MGMT monoclonal antibodies (MGMT and MARP) on stable
and destabilized human non-tumor and tumor-derived cell lines was studied. Conclusions. Studied lectins
are able to modulate the expression of MGMT and MARP. The influence of SNA-I on MARP and MGMT
expression levels depends on origin and genomic stability of cell line. SNA-I is perspective for further study
as potential drug in anti-tumor therapy optimization schemes.
Key words: MGMT expression, MARP expression, lectins, NiCl2, cell lines.
UDC 633.11:631.523:633.11
MOTSNY I.I. 1, SUDARCHUK L.V. 1, GALAEV A.V. 1, CHEBOTAR S.V. 1, 2
1 Plant Breeding and Genetics Institute – National Center of Seed and Cultivar investigations,
Ukraine, 65036, Odessa, Ovidiopolskaya dor., 3, e-mail: motsnyyii@gmail.com
2 Odessa National Mechnikov University, Department of Genetics and Molecular Biology,
Ukraine, 65026, Odessa, Dvoryanskaya str., 2
APPLICATION OF PCR MARKERS FOR DETECTING 1BL.1RS WHEAT-RYE CHROMOSOME
TRANSLOCATIONS AND (1B)1R SUBSTITUTIONS
Nikolai Vavilov was the first to recognize the
utilization of wheat relatives is a promising source
for wheat improvement [1]. As an development of
Vavilov`s ideas a number of wheat introgression
stocks with a high resistance to powdery mildew,
leaf and stem rusts, frost tolerance, high protein
content and some morphological characters has
been obtained as a result of wide crosses [2, 3]. For
a successful practical application the stocks require
an identification of the alien introgressions. DNA
markers become a useful tool for gene or
chromosome identification, especially being
valuable in respect of new for wheat an alien
genetic material.
This paper deals with PCR marker assisted
detection of (1B)1R wheat-rye chromosome
substitution and 1BL.1RS translocation, their meiotic
behavior and genetic analysis of certain alien
characters, incorporated into wheat. The
investigation was carried out within a program for
the development of a genetic collection of bread
wheat lines with qualitative characters.
Material and methods
A set of original primitive introgression
stocks (2n = 42): Erythrospermum 200_97-2 (in
further E200_97-2), Erythrospermum 217_97
(E217_97), Hostianum 242_97-1 (H242_97-1),
Hostianum 242_97-2 (H242_97-2), Hostianum
273_97 (H273_97), Hostianum 274_97 (H274_97)
and ОН232_03, collection sib-strains H74_90-245
and H74_90-258, winter bread wheat cv. Odesskaya
267 (Od267) and F1 hybrids between Od267 and all
the lines have been investigated. The majority of the
stocks were developed from a cross: triticale (8x)
cv. AD825/T. durum Desf. cv. Chernomor and
spontaneous hybridization of the F3 hybrids with the
strain H74_90-245 or H74_90-258, or without it.
Triticale AD825 is a primary amphidiploid (T.
aestivum L. cv. Hostianum 237/S. cereale L. cv.
Voronezhskaya SHI) [4]. The strains H74_90-245
and H74_90-258 were derived in Dobroudja
Agricultural Institute (General Toshevo, Bulgaria)
from the step cross: Dr. Savov`s synthetic (T.
timopheevii Zhuk./Ae. tauschii Coss.)/Tom Pouce
265
Blanc//Avrora/3/Rusalka and received from Dr.
Ivan Panayotov. The stock OH232_03 was obtained
from a cross Od267/H74_90-258.
All lines were analyzed by using DNA-
markers. DNA was isolated from leaf material of
adult plants and seedlings according to standard
CTAB-methods. Because 1RS chromosome
presence, as well as some target gene location were
supposed, the molecular markers: rye
microsatellites: Xrems1303, SR1R003 [5], a secalin-
specific STS-marker – ω-sec-P3 + ω-sec-P4 [6] and
wheat microsatellites: Xgwm18-1BS, Xgwm550-1BS,
Xgwm140-1BL, Xgwm153-1BL, Xgwm357-1AL [7],
Taglut-1AS [8] were chosen for the analysis. PCR
amplification was carried out in a thermocycler
‘Tercik’ (Russia), and a standard electrophoresis
procedure in 10 % poly acrylamide gel (PAAG)
was applied for differentiation of PCR products [9].
Fragment sizes were calculated by comparison with
molecular weight marker pUC19/MspI. 1RS
chromosome presence was detected with the rye
microsatellites and the secalin-specific STS-marker.
Substitution or translocation was identified by the
absence of 1B chromosome corresponding arm via
application of the wheat microsatellites.
Resistance to powdery mildew, leaf and stem
rusts, hairiness of the glumes and leaves was
evaluated within researched material to contain.
Moreover, the stocks, cv. Od267 and the F1s were
studied cytologically with routine acetocarmine
methods. The chromosome substitution or
translocation presence in the stocks and the strains
was confirmed cytologically for meiotic
configurations at metaphase I (MI) in pollen mother
cells (PMCs) of the F1 hybrids.
Plant pathogen resistance was evaluated at
the adult plant stage in field with use of an
international universal scale. Furthermore, powdery
mildew resistance was noted in field in later autumn
at the seedling stage. Leaf and stem rust resistance
were scored both at natural epiphytoty conditions
and under an artificial infection pressure. Herewith,
population mixtures of the most aggressive local
races of both diseases were used. All phenotypical
evaluations were conducted under field conditions
at the heading and flowering stages. Hairiness
(pubescence) was searched on the glumes, upper
(adaxial) and lower surfaces of a leaf blade, as well
as on the leaf margin at the culm node using a
magnifying glass.
Results and discussion
The presence of 1RS chromosome was
detected in the introgression stocks and sib-strains
by the presence of specific products of: Xrems1303,
SR1R003, ω-sec-P3 + ω-sec-P4 markers. The
absence of PCR products with the markers Xgwm18
(1BS), Xgwm550 (1BS), as well as Xgwm140 (1BL)
and Xgwm153 (1BL) permitted to identify 1B
chromosome translocation or substitution. The
detection of PCR-products of the Taglut (1AS) and
Xgwm357 (1AL) markers proved the presence of
intact 1A chromosome in the lines. The
amplification products with the markers Xgwm140
and Xgwm153 were not detected for the stocks
H273_97 and H274_97, but were obtained within
collection sib-strains and for the stocks Е200_97-2,
H217_97, H242_97-1, H242_97-2 and ОН232_03,
as well (Table 1). Thus, the stocks H273_97 and
H274_97 carry (1B)1R substitution, and all other
lines carry 1BL.1RS translocation chromosome.
Table 1. Results of PCR-analysis of the lines studied for the marker loci alleles, bp
Marker locus
O
d2
67
H
74
_9
0-
24
5
H
74
_9
0-
25
8
E2
00
_9
7-
2
E2
17
_9
7
H
24
2_
97
-1
H
24
2_
97
-2
H
27
3_
97
H
27
4_
97
O
H
23
2_
03
Xrems1303 (1RS ) -* 290 290 290 290 290 290 290 290 290
SR1R003 (1RS ) - 97 97 97 97 97 97 97 97 97
ω-sec-P3/P4 (1RS ) - 400 400 400 +#/- 400 400 400 400 400
Xgwm18 (1BS ) 186 - - - 188 - - - - -
Xgwm550 (1BS ) 195 - - - - - - - - -
Xgwm140 (1BL) 223 223,
233
223,
233
223,
233
223,
233 223 223 - - 223,
233
Xgwm153 (1BL) 195 195 195 195 195 195 195 - - 195
Taglut (1AS ) 126 137 134 135 131 128 128 128 128 131
Xgwm357 (1AL) 124 124 124 124 124 124 124 124 124 124
Notes: * – the primer product absence; # Size of DNA amplification fragment in PAAG is more than 400 bp
at the stock E217_97.
266
In general there was no polymorphism of rye
DNA markers among the lines with the
introgressions. Only by using the secalin-specific
ω-sec-P3 + ω-sec-P4 primers a genetic
polymorphism has been detected supposing a new
allele of Sec1 locus in the stock E217_97. The
presence of the product 188 bp with the Xgwm18
marker simultaneously with rye DNA fragments
(Table 1) has proved the translocation heterozygosis
in that stock.
Meiotic observations have supported the
molecular-genetic evidence and have revealed 20
closed bivalents (the maximum) plus an open
bivalent (20II
C + 1II
О) at MI in the F1 hybrids
Od267/translocation stocks (fig. 1, a). Similarly 20
bivalents and 2 univalents (19II
C + 1II
О + 2I) were
observed in the F1s Od267/substitution
stocks (fig. 1, b). The translocation 1BL.1RS
heterozygosis has also been confirmed in the stock
E217_97: some F1 plants Od267/E217_97 had 20II
C
+ 1II
О as the highest meiotic association and the
others – 21II
C (fig. 1, c).
The pairing between short arms of 1R and 1B
chromosomes has not been well documented in
literature. In this study there was no pairing
between 1R and 1B chromosomes in any 322 PMCs
studied in the H273_97/Od267 and
H274_97/Od267 crosses. In the contrast, 21II
C were
observed in 3 meiotic PMCs of 894 (0.3 %) studied
in the F1s between Od267 and the introgression
stocks E200_97-2, E217_97 (plants with 1BL.1RS
translocation), H242_97-1 and H242_97-2.
Therefore, the 1BL.1RS translocation of the stocks
might rarely pair with 1BS chromosome.
Fig. 1. The highest chromosome associations at meiotic MI of F1 hybrids between cv. Od267 and
(a) line Е242_97-1: 20II
C + 1II
О; (b) line H274_97: 19II
C + 1II
О + 2I; (c) line E217_97 (plants without
1BL.1RS translocation): 21II
C (590×)
b
a
c
267
Thereby, investigated introgression stocks
and the collection sib-strains have (1B)1R wheat-
rye chromosome substitution or 1BL.1RS
translocation. That was determined and identified
with use of PCR-markers (Table 1) and confirmed
cytologically (fig. 1). The translocation was
contributed by the collection sib-strains H74_90-
245 and H74_90-258 and derived from Russian
wheat cv. Avrora. Therefore, the rye 1RS
chromosome is originated from Petkus rye. This
chromosome arm transferred to bread wheat genetic
background carries genes, important for the
adaptation of wheat varieties, particularly closely
linked the genes Pm8, Yr9, Lr26 and Sr31 [10]. The
intact rye chromosome 1R for the substitution was
contributed by triticale (8x) cv. AD825 and,
therefore, originated from S. cereale L. cv.
Voronezhskaya SHI. Evidently, such chromosome
rearrangements are known to occur in wheat-rye or
wheat-triticale crosses [11].
Due to their agronomic advantages
translocations with 1RS are usually widespread in
cultivars from Forest-Steppe zone of Ukraine, but
not from South. In South Ukraine 1RS chromosome
has not been used in wheat breeding, because of
traditional to PBGI – NCSCI storage protein
composition selection for the high technological
quality [12]. However, nowadays a program for
wheat-rye translocation use in wheat breeding has
been started [13] and the cvs Zhitnitsa (with
1AL.1RS translocation, leaf and stem rust resistance
and middle quality) and Schedrist` (with 1BL.1RS
translocation and low quality) have been developed.
Depending on karyotype structure the stocks
were considerably distinguished by powdery
mildew, leaf and stem rust resistance and by the
presence of morphological characters (hairy spike
or leaf). The lines E200_97-2, H242_97-1,
H242_97-2, H74_90-245 and H74_90-258, carrying
the 1BL.1RS translocation from cv. Avrora, had high
resistance to all the diseases. There were three and
two genes for resistance, respectively, to leaf and
stem rusts in the lines, and Lr26 and Sr31 among
them [14]. Cv. Od267 was susceptible. The stocks
H273_97 and H274_97 were moderately infected
by powdery mildew and stem rust (MS) and did not
have any leaf rust resistance (S-VS). E217_97 was
somewhat resistant (MS-MR) to powdery mildew
only at the adult plant stage, and ОН232_03 was
susceptible (MS) to stem rust.
As to a pubescence, the presence of typical
wheat Hg1 gene (short and week glume hairiness
like in cv. Ulyanovka) in the stocks of Hostianum
species (H242_97-1, H242_97-2, H273_97 and
H274_97) is determined. The gene is located in 1AS
chromosome [10] and is originated from old cv.
Hostianum 237 – a parental form for the octoploid
triticale AD825. The Hg1 gene coding hairiness of
glumes Mendelian mode of inheritance was
determined: 63 haired: 16 not haired (χ2
3:1 = 0.95)
F2 hybrids in a test-cross with Od267.
As for leaf blade hairiness, the stocks
E217_97, H273_97 and H274_97 were identified as
glabrous ones, and cv. Od267 had a thin layer of
hairs on the adaxial surface. In contrast, the stocks
E200_97-2, H242_97-1 and H242_97-2, as well as
the collection strains H74_90-245 and H74_90-258
were found to carry hairiness on upper and lower
surfaces, as well as on leaf margin at leaf base.
Three major linked genes determining hairiness of
the leaf upper surface (Hlup), lower surface (Hllow)
and leaf margin (Hlm) were revealed with location,
supposedly, on the long arm of chromosome 4D.
The genes were contributed by a synthetic (T.
timopheevii Zhuk./Ae. tauschii Coss) and, therefore,
were originated from T. timopheevii or Ae. tauschii.
The Hlup, Hllow and Hlm loci are non-allelic to Hl
gene. In wheat the alleles Hg and Hl determine
hairiness of glumes or leaf pubescence which
allows them to avoid drought and high temperatures
during the vegetation or grain filling [10].
Conclusion
With use of molecular-genetic and
cytological analyses (1B)1R wheat-rye chromosome
substitution or 1BL.1RS translocation were detected
in the original primitive introgression stocks. The
pairing between 1RS and 1BS chromosomes was
revealed with very low frequency. Three and two
genes for resistance, respectively, to leaf and stem
rusts were revealed, and Lr26 and Sr31 among them
have been recognized and determined to be
somewhat effective. The genes were identified with
the molecular markers Xrems1303, SR1R003,
ω-sec-P3 + ω-sec-P4, contributed by cv. Avrora and
originated from Petkus rye.
The Hg1 gene coding hairiness of glumes
Mendelian mode of inheritance was determined.
Three major linked genes determining hairiness of
the leaf upper surface (Hlup), lower surface (Hllow)
and leaf margin (Hlm) were revealed. The glume
hairiness gene was contributed by the old cv.
Hostianum 237. The leaf pubescence genes were
contributed by a synthetic (T. timopheevii Zhuk./Ae.
tauschii Coss) and, therefore, originated from
T. timopheevii or Ae. tauschii. The Hlup, Hllow and
Hlm loci are non-allelic to Hl1 gene.
268
References
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English summary).
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wheat and amphiploids // Cytology and Genetics (Kiev, Ukraine). 2000. – 34, N 6. – P. 9–20. (in Russian with
English summary).
4. Badaev N.S., Badaeva E.D., Maximov N.G., Volkov D.K., Zelenin A.V. Differences in rye chromosome structure
in the karyotype of triticale // Proc. Ac. Sci. USSR (Dokl. AN SSSR, Moscow: Nauka). – 1982. – 267, N 4. –
P. 953–956. (in Russian).
5. Landjeva S., Korzun V., Tsanev V., Vladova R., Ganeva G. Distribution of the wheat-rye translocation 1BL.1RS
among bread wheat varieties of Bulgaria // Plant Breeding. – 2006. – 125. – P. 102–104.
6. Chai J.F., Zhou R.H., Jia J.Z., Liu X. Development and application of a new codominant PCR marker for detecting
1BL.1RS wheat-rye chromosome translocations // Plant Breeding. – 2006. – 125. – P. 302–304.
7. Rder M.S., Korzun V., Wendehake K., Plaschke J., Tixier M.H., Leroy P., Ganal M.W. A microsatellite map of
wheat // Genetics. – 1998. – 149. – P. 2007–2023.
8. Devos K.M., Bryan G.J., Collins A.J., Stephenson P., Gale M. D. Application of two microsatellite sequences in
wheat storage proteins as molecular markers // Theoretical and Applied Genetics. – 1995. – 90. – P. 247–252.
9. Maniatis T., Fritsch E.E., Sambrook J. Molecular cloning. A laboratory manual. – Cold Spring Harbor Laboratory,
1982. – 480 p.
10. McIntosh R.A., Dubcovsky J., Rogers W.J., Morris C., Appels R., Xia X.C. Catalogue of gene symbols for wheat:
2012 Supplement [E-resource] // Komugi – Integrated wheat science database. – 2012. – Available online:
http://www.shigen.nig.ac.jp/wheat/komugi/genes/symbolClassList.jsp
11. Bartoљ P. Chromosome 1R of rye in wheat breeding // Plant Breeding Abstracts. – 1993. – 63. – P. 1203–1211.
12. Chebotar S. Molecular-genetic analysis of Ukrainian bread wheat gene pool // Abstract book International Plant
Breeding Congress, 10–14 November 2013. – Antalya, Turkey, 2013. – P. 624.
13. Topal N.N. Identification and agronomy characteristic of bread winter wheat varieties and lines with wheat-rye
translocations // Abstracts international scientific conference “Breeding and genetics of agricultural crops:
traditions and prospects”, 17–19 October 2012. – Odesa, Ukraine, 2012. – P. 199–200. (in Ukrainian with English
summary).
14. Sudarchuk L.V., Chebotar S.V. Motsny I.I., Sivolap Yu.M. Hybridological and recombination analysis of alien
characters in F2 introgression hybrids of winter wheat using molecular markers // Coll. Sc. Papers V International
conference “The genome of plants”, 13–16 October 2008 / South Plant Biotechnology Center UAAS (Ukraine). –
Odessa, Ukraine, 2008. – P. 127–130. (in Russian with English summary).
MOTSNY I.I. 1, SUDARCHUK L.V. 1, GALAEV A.V. 1, CHEBOTAR S.V. 1, 2
1 Plant Breeding and Genetics Institute – National Center of Seed and Cultivar investigations, Ukraine,
65036, Odessa, Ovidiopolskaya dor., 3, e-mail: motsnyyii@gmail.com
2 Odessa National Mechnikov University, Department of Genetics and Molecular Biology, Ukraine, 65026,
Odessa, Dvoryanskaya str., 2
APPLICATION OF PCR MARKERS FOR DETECTING 1BL.1RS WHEAT-RYE CHROMOSOME
TRANSLOCATIONS AND (1B)1R SUBSTITUTIONS
Aims. Molecular-genetic and cytological analyses were carried out to detect the alien genes in original
introgression stocks and to investigate their inheritance. Methods. Rye (Xrems1303, SR1R003) and wheat
(Xgwm18-1BS, Xgwm550-1BS, Xgwm140-1BL, Xgwm153-1BL, Xgwm357-1AL, Taglut-1AS) microsatellites
and secalin-specific STS-marker (ω-sec-P3+ ω-sec-P4) have been applied. Results. The (1B)1R wheat-rye
chromosome substitution and 1BL.1RS translocation have been identified. The pairing between short arms of
the 1BL.1RS translocation and of bread wheat chromosome 1B was observed with very low frequency (in
0.3 % PMCs). Conclusions. The stocks have (1B)1R wheat-rye chromosome substitution or 1BL.1RS
translocation. The translocation was contributed by the collection strains, derived from wheat cv. Avrora and
originated from Petkus rye. The intact rye chromosome 1R for the substitution was contributed by triticale
(8x) cv. AD825 and originated from rye Voronezhskaya SHI. The substitution stocks were susceptible to leaf
and stem rusts because of another origination of the 1R chromosome. Three major linked genes determining
hairiness of the leaf upper surface (Hlup), lower surface (Hllow) and leaf margin (Hlm) were revealed. The
genes were contributed by a synthetic (T. timopheevii/Ae. tauschii) and were non-allelic to Hl1 gene.
Key words: Triticum aestivum, (1B)1R substitution, 1BL.1RS translocation, hairy leaf, PCR-markers.
|
| id | nasplib_isofts_kiev_ua-123456789-178349 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 2219-3782 |
| language | English |
| last_indexed | 2025-12-07T18:42:36Z |
| publishDate | 2014 |
| publisher | Інститут молекулярної біології і генетики НАН України |
| record_format | dspace |
| spelling | Motsny, I.I. Sudarchuk, L.V. Galaev, A.V. Chebotar, S.V. 2021-02-18T15:26:04Z 2021-02-18T15:26:04Z 2014 Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions / I.I. Motsny, L.V. Sudarchuk, A.V. Galaev, S.V. Chebotar // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2014. — Т. 15. — С. 264-268. — Бібліогр.: 14 назв. — англ. 2219-3782 https://nasplib.isofts.kiev.ua/handle/123456789/178349 633.11:631.523:633.11 Aims. Molecular-genetic and cytological analyses were carried out to detect the alien genes in original introgression stocks and to investigate their inheritance. Methods. Rye (Xrems1303, SR1R003) and wheat (Xgwm18-1BS, Xgwm550-1BS, Xgwm140-1BL, Xgwm153-1BL, Xgwm357-1AL, Taglut-1AS) microsatellites and secalin-specific STS-marker (ω-sec-P3+ ω-sec-P4) have been applied. Results. The (1B)1R wheat-rye chromosome substitution and 1BL.1RS translocation have been identified. The pairing between short arms of the 1BL.1RS translocation and of bread wheat chromosome 1B was observed with very low frequency (in 0.3 % PMCs). Conclusions. The stocks have (1B)1R wheat-rye chromosome substitution or 1BL.1RS translocation. The translocation was contributed by the collection strains, derived from wheat cv. Avrora and originated from Petkus rye. The intact rye chromosome 1R for the substitution was contributed by triticale (8x) cv. AD825 and originated from rye Voronezhskaya SHI. The substitution stocks were susceptible to leaf and stem rusts because of another origination of the 1R chromosome. Three major linked genes determining hairiness of the leaf upper surface (Hlup), lower surface (Hllow) and leaf margin (Hlm) were revealed. The genes were contributed by a synthetic (T. timopheevii/Ae. tauschii) and were non-allelic to Hl1 gene.
 
 Key words: Triticum aestivum, (1B)1R substitution, 1BL.1RS translocation, hairy leaf, PCR-markers. en Інститут молекулярної біології і генетики НАН України Фактори експериментальної еволюції організмів Прикладна генетика і селекція Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions Article published earlier |
| spellingShingle | Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions Motsny, I.I. Sudarchuk, L.V. Galaev, A.V. Chebotar, S.V. Прикладна генетика і селекція |
| title | Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions |
| title_full | Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions |
| title_fullStr | Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions |
| title_full_unstemmed | Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions |
| title_short | Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions |
| title_sort | application of pcr markers for detecting 1bl.1rs wheat-rye chromosome translocations and (1b)1r substitutions |
| topic | Прикладна генетика і селекція |
| topic_facet | Прикладна генетика і селекція |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/178349 |
| work_keys_str_mv | AT motsnyii applicationofpcrmarkersfordetecting1bl1rswheatryechromosometranslocationsand1b1rsubstitutions AT sudarchuklv applicationofpcrmarkersfordetecting1bl1rswheatryechromosometranslocationsand1b1rsubstitutions AT galaevav applicationofpcrmarkersfordetecting1bl1rswheatryechromosometranslocationsand1b1rsubstitutions AT chebotarsv applicationofpcrmarkersfordetecting1bl1rswheatryechromosometranslocationsand1b1rsubstitutions |