Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions
Aims. Molecular-genetic and cytological analyses were carried out to detect the alien genes in original introgression stocks and to investigate their inheritance. Methods. Rye (Xrems1303, SR1R003) and wheat (Xgwm18-1BS, Xgwm550-1BS, Xgwm140-1BL, Xgwm153-1BL, Xgwm357-1AL, Taglut-1AS) microsatellites...
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| Опубліковано в: : | Фактори експериментальної еволюції організмів |
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| Дата: | 2014 |
| Автори: | , , , |
| Формат: | Стаття |
| Мова: | Англійська |
| Опубліковано: |
Інститут молекулярної біології і генетики НАН України
2014
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| Онлайн доступ: | https://nasplib.isofts.kiev.ua/handle/123456789/178349 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions / I.I. Motsny, L.V. Sudarchuk, A.V. Galaev, S.V. Chebotar // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2014. — Т. 15. — С. 264-268. — Бібліогр.: 14 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862723581316694016 |
|---|---|
| author | Motsny, I.I. Sudarchuk, L.V. Galaev, A.V. Chebotar, S.V. |
| author_facet | Motsny, I.I. Sudarchuk, L.V. Galaev, A.V. Chebotar, S.V. |
| citation_txt | Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions / I.I. Motsny, L.V. Sudarchuk, A.V. Galaev, S.V. Chebotar // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2014. — Т. 15. — С. 264-268. — Бібліогр.: 14 назв. — англ. |
| collection | DSpace DC |
| container_title | Фактори експериментальної еволюції організмів |
| description | Aims. Molecular-genetic and cytological analyses were carried out to detect the alien genes in original introgression stocks and to investigate their inheritance. Methods. Rye (Xrems1303, SR1R003) and wheat (Xgwm18-1BS, Xgwm550-1BS, Xgwm140-1BL, Xgwm153-1BL, Xgwm357-1AL, Taglut-1AS) microsatellites and secalin-specific STS-marker (ω-sec-P3+ ω-sec-P4) have been applied. Results. The (1B)1R wheat-rye chromosome substitution and 1BL.1RS translocation have been identified. The pairing between short arms of the 1BL.1RS translocation and of bread wheat chromosome 1B was observed with very low frequency (in 0.3 % PMCs). Conclusions. The stocks have (1B)1R wheat-rye chromosome substitution or 1BL.1RS translocation. The translocation was contributed by the collection strains, derived from wheat cv. Avrora and originated from Petkus rye. The intact rye chromosome 1R for the substitution was contributed by triticale (8x) cv. AD825 and originated from rye Voronezhskaya SHI. The substitution stocks were susceptible to leaf and stem rusts because of another origination of the 1R chromosome. Three major linked genes determining hairiness of the leaf upper surface (Hlup), lower surface (Hllow) and leaf margin (Hlm) were revealed. The genes were contributed by a synthetic (T. timopheevii/Ae. tauschii) and were non-allelic to Hl1 gene.

Key words: Triticum aestivum, (1B)1R substitution, 1BL.1RS translocation, hairy leaf, PCR-markers.
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| first_indexed | 2025-12-07T18:42:36Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-178349 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 2219-3782 |
| language | English |
| last_indexed | 2025-12-07T18:42:36Z |
| publishDate | 2014 |
| publisher | Інститут молекулярної біології і генетики НАН України |
| record_format | dspace |
| spelling | Motsny, I.I. Sudarchuk, L.V. Galaev, A.V. Chebotar, S.V. 2021-02-18T15:26:04Z 2021-02-18T15:26:04Z 2014 Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions / I.I. Motsny, L.V. Sudarchuk, A.V. Galaev, S.V. Chebotar // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2014. — Т. 15. — С. 264-268. — Бібліогр.: 14 назв. — англ. 2219-3782 https://nasplib.isofts.kiev.ua/handle/123456789/178349 633.11:631.523:633.11 Aims. Molecular-genetic and cytological analyses were carried out to detect the alien genes in original introgression stocks and to investigate their inheritance. Methods. Rye (Xrems1303, SR1R003) and wheat (Xgwm18-1BS, Xgwm550-1BS, Xgwm140-1BL, Xgwm153-1BL, Xgwm357-1AL, Taglut-1AS) microsatellites and secalin-specific STS-marker (ω-sec-P3+ ω-sec-P4) have been applied. Results. The (1B)1R wheat-rye chromosome substitution and 1BL.1RS translocation have been identified. The pairing between short arms of the 1BL.1RS translocation and of bread wheat chromosome 1B was observed with very low frequency (in 0.3 % PMCs). Conclusions. The stocks have (1B)1R wheat-rye chromosome substitution or 1BL.1RS translocation. The translocation was contributed by the collection strains, derived from wheat cv. Avrora and originated from Petkus rye. The intact rye chromosome 1R for the substitution was contributed by triticale (8x) cv. AD825 and originated from rye Voronezhskaya SHI. The substitution stocks were susceptible to leaf and stem rusts because of another origination of the 1R chromosome. Three major linked genes determining hairiness of the leaf upper surface (Hlup), lower surface (Hllow) and leaf margin (Hlm) were revealed. The genes were contributed by a synthetic (T. timopheevii/Ae. tauschii) and were non-allelic to Hl1 gene.
 
 Key words: Triticum aestivum, (1B)1R substitution, 1BL.1RS translocation, hairy leaf, PCR-markers. en Інститут молекулярної біології і генетики НАН України Фактори експериментальної еволюції організмів Прикладна генетика і селекція Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions Article published earlier |
| spellingShingle | Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions Motsny, I.I. Sudarchuk, L.V. Galaev, A.V. Chebotar, S.V. Прикладна генетика і селекція |
| title | Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions |
| title_full | Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions |
| title_fullStr | Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions |
| title_full_unstemmed | Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions |
| title_short | Application of PCR markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1B)1R substitutions |
| title_sort | application of pcr markers for detecting 1bl.1rs wheat-rye chromosome translocations and (1b)1r substitutions |
| topic | Прикладна генетика і селекція |
| topic_facet | Прикладна генетика і селекція |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/178349 |
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