Role of cytokinins in the regulation the chilling stress response in Triticum aestivum and Triticum spelta
The research is devoted to the study of the eff ect of chilling (+4 °C, 2 h) on cytokinin homeostasis in 14-day stressed and 21-day restored plants of winter wheat (Triticum aestivum L.) cv. “Podolyanka” and spelt wheat (Triticum spelta L.) cv. “Frankenkorn”. Our study revealed that chilling induces...
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| Цитувати: | Role of cytokinins in the regulation the chilling stress response in Triticum aestivum and Triticum spelta / L.V. Voytenko, M.M. Shcherbatiuk, V.A. Vasyuk, I.V. Kosakivska // Доповіді Національної академії наук України. — 2024. — № 3. — С. 69-76. — Бібліогр.: 22 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1859719391918161920 |
|---|---|
| author | Voytenko, L.V. Shcherbatiuk, M.M. Vasyuk, V.A. Kosakivska, I.V. |
| author_facet | Voytenko, L.V. Shcherbatiuk, M.M. Vasyuk, V.A. Kosakivska, I.V. |
| citation_txt | Role of cytokinins in the regulation the chilling stress response in Triticum aestivum and Triticum spelta / L.V. Voytenko, M.M. Shcherbatiuk, V.A. Vasyuk, I.V. Kosakivska // Доповіді Національної академії наук України. — 2024. — № 3. — С. 69-76. — Бібліогр.: 22 назв. — англ. |
| collection | DSpace DC |
| container_title | Доповіді НАН України |
| description | The research is devoted to the study of the eff ect of chilling (+4 °C, 2 h) on cytokinin homeostasis in 14-day stressed and 21-day restored plants of winter wheat (Triticum aestivum L.) cv. “Podolyanka” and spelt wheat (Triticum spelta L.) cv. “Frankenkorn”. Our study revealed that chilling induces complex changes in the content and distribution of cytokinins in plants that are species- and organ-specifi c. Aft er chilling, the total cytokinin content in the roots of winter wheat cv. “Podolyanka” increased threefold due to the accumulation of trans-zeatin-O-glucoside, trans-zeatin, isopentenyladenine and isopentenyladenosine. Stress did not aff ect trans-zeatin riboside accumulation in roots but induced a fourfold increase in its content in the shoots of winter wheat cv. “Podolyanka”. Th e total content of cytokinin in the roots of spelt wheat cv. “Frankenkorn” decreased by 1.4 times due to decline in trans-zeatin-O-glucoside and trans-zeatin riboside content, while in shoots, it decreased by 1.2 times owing to reduced trans-zeatin-O-glucoside and isopentenyladenine levels. Prolonged exposure to chilling was manifested by an increase in hormone levels in both 21-day-old species. Our data revealed common and distinct traits in cytokinin homeostasis between winter wheat cv. “Podolyanka” and spelt wheat cv. “Frankenkorn” during rapid adaptation and the recovery period, providing new insights into the response of these species to chilling.
Робота присвячена дослідженню впливу охолодження (+4 °C, 2 год) на гомеостаз цитокінінів у 14-добових стресованих та 21-добових відновлених рослин озимої пшениці (Triticum aestivum L.) сорту “Подолянка” та спельти (Triticum spelta L.) сорту “Франкенкорн”. Показано, що охолодження зумовлює комплексні зміни у вмісті і розподілі цитокінінів, які є видо- та органоспецифічними. Після охолодження загальний вміст цитокінінів (нг/г сирої речовини) у коренях пшениці зріс утричі за рахунок накопичення транс-зеатин-O- глюкозиду, транс-зеатину, ізопентеніладеніну та ізопентеніладенозину. Стрес не вплинув на накопичення транс-зеатинрибозиду у коренях, але спричинив збільшення його вмісту в чотири рази в надземній частині озимої пшениці “Подолянка”. Загальний вміст цитокінінів у коренях спельти “Франкенкорн” зменшився в 1,4 раза за рахунок зниження вмісту транс-зеатин-O-глюкозиду і транс-зеатинрибозиду, а в надземній частині — в 1,2 раза за рахунок зниження рівня транс-зеатин-O-глюкозиду та ізопентеніладеніну. Віддалений ефект охолодження виявився у підвищенні рівня цитокінінів у обох видах рослин. Визначені загальні і специфічні зміни цитокінінового гомеостазу озимої пшениці “Подолянка” та спельти “Франкенкорн” під час швидкої адаптації і в період відновлення додають нові знання про реакцію споріднених видів пшениці на охолодження на ранніх етапах вегетації.
|
| first_indexed | 2025-12-01T08:53:26Z |
| format | Article |
| fulltext |
69
ОПОВІДІ
НАЦІОНАЛЬНОЇ
АКАДЕМІЇ НАУК
УКРАЇНИ
ISSN 1025-6415. Допов. Нац. акад. наук Укр. 2024. № 3: 69—76
C i t a t i o n: Voytenko L.V., Shcherbatiuk M.M., Vasyuk V.A., Kosakivska I.V. Role of cytokinins in the regulation the
chilling stress response in Triticum aestivum and Triticum spelta. Dopov. Nac. akad. nauk Ukr. 2024. No. 3. P. 69—76.
https://doi.org/10.15407/dopovidi2024.03.069
© Publisher PH «Akademperiodyka» of the NAS of Ukraine, 2024. Th is is an open acsess article under the CC BY-NC-
ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/)
БІОЛОГІЯ
BIOLOGY
https://doi.org/10.15407/dopovidi2024.03.069
UDC 58.036:577.175.1:582.542.1
L.V. Voytenko, https://orcid.org/0000-0003-0380-0807
M.M. Shcherbatiuk, https://orcid.org/0000-0002-6453-228X
V.A. Vasyuk, https://orcid.org/0000-0003-1069-9698
I.V. Kosakivska, https://orcid.org/0000-0002-2173-8341
M.G. Kholodny Institute of Botany of the NAS of Ukraine, Kyiv, Ukraine
E-mail: lesyavoytenko@gmail.com, chrom.botany@ukr.net, vasyuk@ukr.net, irynakosakivska@gmail.com
Role of cytokinins in the regulation
the chilling stress response in Triticum aestivum and Triticum spelta
Presented by Academician of the NAS of Ukraine V.V. Morgun
Th e research is devoted to the study of the eff ect of chilling (+4 °C, 2 h) on cytokinin homeostasis in 14-day stressed
and 21-day restored plants of winter wheat (Triticum aestivum L.) cv. “Podolyanka” and spelt wheat (Triticum
spelta L.) cv. “Frankenkorn”. Our study revealed that chilling induces complex changes in the content and distribution
of cytokinins in plants that are species- and organ-specifi c. Aft er chilling, the total cytokinin content in the roots of
winter wheat cv. “Podolyanka” increased threefold due to the accumulation of trans-zeatin-O-glucoside, trans-zeatin,
isopentenyladenine and isopentenyladenosine. Stress did not aff ect trans-zeatin riboside accumulation in roots but
induced a fourfold increase in its content in the shoots of winter wheat cv. “Podolyanka”. Th e total content of cytokinin
in the roots of spelt wheat cv. “Frankenkorn” decreased by 1.4 times due to decline in trans-zeatin-O-glucoside and
trans-zeatin riboside content, while in shoots, it decreased by 1.2 times owing to reduced trans-zeatin-O-glucoside
and isopentenyladenine levels. Prolonged exposure to chilling was manifested by an increase in hormone levels in both
21-day-old species. Our data revealed common and distinct traits in cytokinin homeostasis between winter wheat cv.
“Podolyanka” and spelt wheat cv. “Frankenkorn” during rapid adaptation and the recovery period, providing new
insights into the response of these species to chilling.
Keywords: Triticum aestivum, Triticum spelta, cytokinins, chilling, recovery.
Introduction. Abiotic stresses have a detrimental eff ect on the growth and productivity of cereal
crops, signifi cantly aff ecting the world’s most vital group of monocotyledonous plants that have
served as food and other basic human needs for millennia. Th ese stresses cause approximately
50% of crop losses, with low temperatures alone accounting for 7% [1]. Annually, 85% of the
70 ISSN 1025-6415. Dopov. Nac. akad. nauk Ukr. 2024. No. 3
L.V. Voytenko, M.M. Shcherbatiuk, V.A. Vasyuk, I.V. Kosakivska
world’s wheat acreage is aff ected by spring frosts, which mainly occur in March and April during
early development [2]. Low tempe ratures adversely aff ect vegetative and reproductive growth
of wheat, delay seed germination and lead to partial death of plants due to impaired embryo
development. Th e rate of water and nutrients absorption by seedlings decreases, which leads to
dehydration of cells, disruption of nutrient processes and yield losses in the range of 10—30% [3].
Cold stress in wheat plants alters photosynthesis, respiration and substances transport intensity
[4], inducing structural and functional reorganization of photosynthetic and energy apparatus [5],
and triggered changes in endogenous phytohormone balance [6].
Phytohormones are important endogenous regulators of physiological and metabolic pro-
cesses in plants under both normal and stressful conditions [7, 8]. Th ey represent promising tar-
gets for biotechnological approaches aimed at improving and increasing plant productivity in
desired direction. Current research in fi eld of phytohormones is mainly focused on cell and tis-
sue culture and modern omics approaches [9]. Recently, phytohormone engineering has emerged
as an important platform for the development of stress-tolerant plants. However, the success of
phytohormone utilization depends on the fundamental study of the mechanisms of stress toler-
ance enhancement. Phytohormones belonging to the cytokinin family play a crucial role in the
regulation of plant growth and development. Th ey control cell division, meristem formation, pho-
tosynthesis, aging and the uptake of macro- and microelements [10—12]. Cytokinin synthesis
primarily occurs in the root apical meristem, with the hormone translocating to the aerial part
via the transpiration stream. Zeatin-type cytokinins, including trans- and cis-zeatin (t-Z, c-Z),
trans-zeatin riboside (t-ZR), and zeatin-O-glucoside (ZOG), are predominant in the xylem, while
isopentenyl-type cytokinins, such as isopentenyladenine (iP) and isopentenyladenosine (iPA) are
prevalent in the phloem [13]. Recent studies have increasingly demonstrated the ability of cy-
tokinins to mitigate both abiotic and biotic stressors [11, 14, 16]. However, the precise role of
cytokinins in response of plants to low temperatures remains incompletely understood. Our study
aims to investigate the involvement of endogenous cytokinins in the formation of the responses to
short-term chilling in the related wheat species Triticum aestivum and T. spelta.
Materials and method. Fourteen- and twenty-one-day-old plants of winter wheat (Triticum
aestivum L. cv. “Podolyanka”) and spelt wheat (Triticum spelta L. cv. “Frankenkorn”) were examined
under laboratory conditions from 2022 to 2023. Th e “Podolyanka” wheat variety is known for its
winter hardiness and drought resistance, high yield and adaptability to various growing conditions,
while “Frankenkorn” spelt wheat variety exhibits frost resistance and environmental versatility [16,
17]. Calibrated seeds were sterilized in 80% ethanol solution, washed with distilled water, placed
in cuvettes fi lled with water for three hours and germinated in a thermostat at a temperature of
+24 °C for 21 hours. Th e germinated seeds were then planted in 2-liter containers fi lled with
calcined river sand. Plants were grown under controlled conditions at a temperature of +20 °С, a
light intensity of 190 μmol/(m2·s), a photoperiod of 16/8 h (day/night), and relative air humidity
65 ± 5%. Substrate moisture was maintained at 60% of full moisture capacity with daily watering
using Knop’s solution at a rate of 50 ml per vessel. Fourteen-day-old plants with 2-3 leaves were di-
vided into two groups: one group was exposed to +4 °С temperature 190 μmol/(m2·s) light inten-
sity for 2 hours (LT-plants), and the other group, which served as a control (C-plants), continued
to grow under initial experimental conditions. Plants were recovered by growing under controlled
conditions until the twenty-fi rst day (3-4 leaf phase). Shoots and roots of both 14- and 21-day-old
LT- and C-plants were selected for the study.
71ISSN 1025-6415. Допов. Нац. акад. наук Укр. 2024. № 3
Role of cytokinins in the regulation the chilling stress response in Triticum aestivum and Triticum spelta
Extraction, purifi cation and quantitative determination of endogenous t-Z, t-ZR, t-ZOG, iPA
and iP were performed following the method outlined in article [18].
Th e experiments were conducted with three biological and three analytical replicates. Analy-
sis and calculation of the phytohormone content were performed using Agilent OpenLAB CDS
ChemStation Edition (rev. C.01.09) chromatograph soft ware. Statistical analysis was performed
using the program Statistix v. 10.0 (“Analytical Soft ware”, USA). One-way analysis of variance
(ANOVA) was used, and diff erences between mean values were considered signifi cant at P ≤ 0.05.
Results and discussion. Five forms of cytokinins were identifi ed in “Podolyanka” winter
wheat and “Frankenkorn” spelt wheat plants: t-Z, t-ZR, t-ZOG, iPA, and iP. Active forms of cytoki-
nins t-Z and iP were predominant in 14-day-old control winter wheat plants, with their content in
shoots being 1.2 and 6.6 times higher than in roots, respectively. Th e level of t-ZR approached the
limit of the method sensitivity (Fig. 1). In 14-day-old control spelt wheat plants, t-ZR dominated
among the active forms, with content in shoots and roots at 62.2 ± 3.1 and 54.8 ± 2.7 ng/g FW
(fresh weight), respectively. In spelt wheat roots, the level of t-ZОG was 1.9 times higher, while
in winter wheat, it was 2.6 times lower than in shoots. iPA in the organs of both species was
present in trace amounts (Fig. 2). In shoots and roots of 21-day-old control winter wheat plants,
the total cytokinin content increased by 59.4% and 79 %, amounting to 542.4 ± 27.1 ng/g FW and
214.8 ± 10.7 ng/g FW, respectively. Accumulation of t-ZOG, t-Z and iPA was observed in both
organs, with amounts in shoots being 0.9, 1.6 and 2.4 times higher than in roots (see Fig. 1).
In shoots and roots of 21-day-old control spelt wheat plants, the total cytokinin content in-
creased by 145,6% and 172.2%, amounting to 336.0 ± 16.8 ng/g FW and 434.5 ± 21.7 ng/g FW,
respectively. t-ZOG, t-ZR and iPA were accumulated in both organs of spelt wheat plants. Th e
levels of t-ZOG and iPA in roots were 2.2 and 3.3 times higher than in shoots, whereas the con-
tents of t-Z, t-ZR and iPA were in a close range (see Fig. 2). Among the isopentenyl forms, iP
(225.6 ± 11.38 ng/g FW) was predominant in shoots of 21-day-old control winter wheat plants,
while iPA was predominant in spelt wheat roots (73.8 ± 3.4 ng/g FW) (see Fig. 1, 2).
ng
/g
fr
es
h
w
ei
gh
t
t-Z
O
G
t-Z
O
G
t-Z
R
t-Z
Rt-Z t-ZiP iPiP
A
iP
A
t-Z
O
G
t-Z
O
G
t-Z
R
t-Z
Rt-Z t-ZiP iPiP
A
iP
A
280 Shoots
Roots
210
*
*
*
*
*
*
*
*
140
70
Control
14 days
Chilling stress Control
21 days
Recovery
0
Fig. 1. Accumulation and distribution of endogenous cytokinins in Triticum
aestivum L. cv. “Podolyanka” plants after chilling stress (+4 °С, 2 h) and during
recovery period (ng/g FW). іP — isopentenyladenine; іPА — iso pen tenyl-
adenosine; t-Z — trans-zeatin; t-ZR — trans-zeatin riboside; t-ZOG — trans-
zeatin-O-glucoside. * — significant difference at P ≤ 0.05 vs. control; data are
the mean ± SE, n = 9
72 ISSN 1025-6415. Dopov. Nac. akad. nauk Ukr. 2024. No. 3
L.V. Voytenko, M.M. Shcherbatiuk, V.A. Vasyuk, I.V. Kosakivska
Aft er short-term chilling, the total cytokinin content in the roots of 14-day-old winter wheat
plants increased threefold, reaching 377.6 ± 18.9 ng/g FW. Th is increase was due to the increase
of t-ZOG, t-Z, iP and iPA content by 4.2-fold, 1.7-fold, 6.4-fold and 2.0-fold, respectively. In
shoots, the total cytokinin content remained elevated and amounted to 349.1 ± 17.5 ng/g FW. Low
positive temperature did not aff ect the t-ZR accumulation in roots, but led to fourfold increase
of the hormone level in shoots (see Fig. 1). Conversely, in 14-day-old spelt wheat plants, chilling
caused a 1.5-fold and 1.2-fold decrease in total cytokinin content in roots and shoots, respectively,
amounting to 103.9 ± 5.2 ng/g FW and 114.9±5.7 ng/g FW. Th e changes in roots were attributed
to 1.6-fold and 1.7-fold reduction in t-ZOG and t-ZR levels, while shoot alterations involved a 2.3-
fold and 5.4-fold decrease in t-ZOG and iP levels. Th e content of t-Z in both organs, t-ZR in shoots
and both isopentenyl forms in roots remained within the control range (see Fig. 2).
During the recovery period, the total cytokinin content in the shoots of 21-day-old winter
wheat plants increased by 75.9% to 613.9 ± 30.7 ng/g FW, while in roots it decreased by 18.2%
and amounted to 309.1 ± 15.5 ng/g FW. Th ese values were 13.2% and 43.9% higher than the
corresponding values in unstressed 21-day-old control plants. Th e recovered plant shoots were
dominated by t-ZOG, iP and t-Z, which were 245.4 ± 12.3 ng/g FW, 231.8 ± 11.6 ng/g FW, and
104.3 ± 5.2 ng/g FW. t-ZOG and iP exceeded controls by 47,9% and 2,7%, while t-Z content was
8.3% lower. In recovered plants roots, t-ZOG content was 53.6% higher than control, reaching
221.6 ± 11,1 ng/g FW. t-Z, t-ZR and iPA levels in shoots and t-Z and iPA levels in the roots did not
reach control values (see Fig. 1).
In shoots and roots of spelt wheat plants recovered at 21 days aft er stress, total cytokinins
content increased by 20.7% and 23.8%, reaching 138.7 ± 6.9 ng/g FW and 128.6 ± 6.4 ng/g FW,
respectively. Th ese values were 58.7% and 70.4% below the control values. t-ZOG and t-ZR
dominated in recovered plants, distributed evenly between shoots and roots. Th eir levels were
30.5% and 73.9% lower in shoots and 32.6% and 67.9% in roots compared to control. t-Z content
in shoots and roots of recovered plants was at control levels, while isopentenyl form levels were
lower (see Fig. 2).
ng
/g
fr
es
h
w
ei
gh
t
t-Z
O
G
t-Z
O
G
t-Z
R
t-Z
Rt-Z t-ZiP iPiP
A
iP
A
t-Z
O
G
t-Z
O
G
t-Z
R
t-Z
Rt-Z t-ZiP iPiP
A
iP
A
280 Shoots
Roots
210
*
* *
* **
*
*
*
140
70
Control
14 days
Chilling stress Control
21 days
Recovery
0
Fig. 2. Accumulation and distribution of endogenous cytokinins in Triticum
spelta L. cv. “Frankenkorn” plants after chilling stress (+4 °С, 2 h) and during
recovery period (ng/g FW). іP — isopentenyladenine; іPА — isopentenyl ade-
nosine; t-Z — trans-zeatin; t-ZR — trans-zeatin riboside; t-ZOG — trans-
zeatin-O-glucoside. * — significant difference at P ≤ 0.05 vs. control; data are
the mean ± SE, n = 9
73ISSN 1025-6415. Допов. Нац. акад. наук Укр. 2024. № 3
Role of cytokinins in the regulation the chilling stress response in Triticum aestivum and Triticum spelta
In general, the total cytokinin content in 14-day control spelt wheat plants was 1.6 times lower
than in winter wheat. By the day 21, the accumulation of cytokinins in both species was equally
high. When exposed to low positive temperature, the cytokinin content in 14-day-old winter
wheat plants increased 1.6 times, while in spelt it decreased 1.4 times. Aft er recovery, the level
of cytokinins in 21-day-old winter wheat and spelt wheat plants increased by 1.3 and 1.2 times.
Winter wheat plants exhibited a 21.9% higher total cytokinin content, while spelt wheat showed a
65.3% lower content compared to control non-stressed plants (Table).
We previously demonstrated that aft er short-term chilling (+2 °C, 2 h), the total cytokinin
content in the roots and shoots of 14-day-old winter wheat plants of the frost-resistant variety
“Volodarka” decreased. Hormone accumulation occurred mainly in shoots, with t-ZR predom-
inating in roots and t-Z in shoots. Additionally, we established that aft er chilling, the level of
zeatin-type cytokinins signifi cantly decreased in the roots of the heat-resistant winter wheat vari-
ety “Yatran 60”, while it increased in the shoots [19]. Other researchers reported that under low-
temperature stress in Triticum monococcum wheat plants, cytokinin levels decreased, but during
the adaptation phase (aft er 21 days), active hormone forms increased, reaching maximum values
[20]. In response to chilling (+4 °C), bioactive cytokinin levels decreased in winter and spring
wheat plants. Aft er 3—7 days of exposure to low positive temperature, endogenous cytokinin
levels increased, but diminished by the 21st day, with winter wheat plants showing faster and more
pronounced responses [21]. Studies of cytokinin content changes during short- and long-term
low-temperature treatment revealed signifi cant diff erences between sensitive and cold-resistant
wheat genotypes. Th e cold-resistant genotype showed decreased levels of active cytokinin forms
in response to cold stress, accompanied by activation of the gene encoding isopentenyltransferase,
a key enzyme cytokinin biosynthesis [22].
Conclusions. Our study revealed that aft er short-term chilling stress, the total cytokinin
content in the roots of 14-day-old winter wheat plants increased threefold due to de novo
biosynthesis and accumulation of cytokinins, such as t-ZOG, t-Z, iP and iPA. Low positive tem-
perature did not aff ect t-ZR accumulation in roots, but induced a fourfold increase in its content
in winter wheat shoots. Conversely, aft er exposure to low positive temperature, the total cytokinin
content in the roots of 14-day-old spelt wheat plants decreased by 1.4-fold due to a decline in t-
ZOG and t-ZR content, while in shoots, it decreased by 1.2-fold due to reduction in t-ZOG and
iP levels. Cytokinins have been shown to promote shoot growth and simultaneously inhibit root
system growth. We believe that the increase in the content of endogenous cytokinins in the roots
of winter wheat plants aft er chilling mediates the inhibition of growth processes, which increases
Total cytokinin content in Triticum aestivum L. cv “Podolyanka”
and Triticum spelta L. cv “Frankenkorn” plants exposed to chilling stress (+4 oC, 2 hour)
and in recovery period, ng/g FW
Option experiment
14-day-old 21-day-old
С-plants LT-plants С-plants Aft er recovery
Triticum aestivum 460.2 ± 23.1 726.7 ± 36.3* 757.2 ± 37.9 922.9 ± 46.1*
Triticum spelta 296.4 ± 14.8 218.8 ± 10.9* 770.5 ± 38.5 267.3 ± 13.4*
Note. Data are the mean ± SE, n = 9. * Significant difference at P ≤ 0.05 vs. control.
74 ISSN 1025-6415. Dopov. Nac. akad. nauk Ukr. 2024. No. 3
L.V. Voytenko, M.M. Shcherbatiuk, V.A. Vasyuk, I.V. Kosakivska
resistance. Th e prolonged eff ect of chilling was manifested by an increase in the level of the hor-
mone in both 21-day-old species. In general, chilling stress caused complex changes in the content
and distribution of cytokinins, the characteristics of which depended on the species and plant
organ. Our data revealed common features as well as organ and species specifi cities of cytokinin
homeostasis of winter wheat and spelt plants during rapid adaptation to chilling stress and during
the recovery period, which provided new insights into the response of these related wheat species
to low positive temperature at early vegetative stages.
Th e publication contains the results of research carried out as part of the project funded by the
National Academy of Sciences of Ukraine № ІІІ-90-19.463 “Hormonal regulation of growth and de-
velopment of cereal plants under the infl uence of negative climatic factors” (2019—2023).
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Received 27.02.2024
76 ISSN 1025-6415. Dopov. Nac. akad. nauk Ukr. 2024. No. 3
L.V. Voytenko, M.M. Shcherbatiuk, V.A. Vasyuk, I.V. Kosakivska
Л.В. Войтенко, https://orcid.org/0000-0003-0380-0807
М.М. Щербатюк, https://orcid.org/0000-0002-6453-228X
В.А. Васюк, https://orcid.org/0000-0003-1069-9698
І.В. Косаківська, https://orcid.org/0000-0002-2173-8341
Інститут ботаніки ім. М.Г. Холодного НАН України, Київ, Україна
E-mail: lesyavoytenko@gmail.com, chrom.botany@ukr.net, vasyuk@ukr.net, irynakosakivska@gmail.com
ЦИТОКІНІНИ У ФОРМУВАННІ РЕАКЦІЇ-ВІДПОВІДІ НА ОХОЛОДЖЕННЯ
В РОСЛИНАХ TRITICUM AESTIVUM І TRITICUM SPELTA
Робота присвячена дослідженню впливу охолодження (+4 °C, 2 год) на гомеостаз цитокінінів у 14-добових
стресованих та 21-добових відновлених рослин озимої пшениці (Triticum aestivum L.) сорту “Подолянка” та
спельти (Triticum spelta L.) сорту “Франкенкорн”. Показано, що охолодження зумовлює комплексні зміни у
вмісті і розподілі цитокінінів, які є видо- та органоспецифічними. Після охолодження загальний вміст
цитокінінів (нг/г сирої речовини) у коренях пшениці зріс утричі за рахунок накопичення транс-зеатин-O-
глюкозиду, транс-зеатину, ізопентеніладеніну та ізопентеніладенозину. Стрес не вплинув на накопичення
транс-зеатинрибозиду у коренях, але спричинив збільшення його вмісту в чотири рази в надземній части-
ні озимої пшениці “Подолянка”. Загальний вміст цитокінінів у коренях спельти “Франкенкорн” зменшився
в 1,4 раза за рахунок зниження вмісту транс-зеатин-O-глюкозиду і транс-зеатинрибозиду, а в надземній
частині — в 1,2 раза за рахунок зниження рівня транс-зеатин-O-глюкозиду та ізопентеніладеніну. Віддале-
ний ефект охолодження виявився у підвищенні рівня цитокінінів у обох видах рослин. Визначені загальні
і специфічні зміни цитокінінового гомеостазу озимої пшениці “Подолянка” та спельти “Франкенкорн” під
час швидкої адаптації і в період відновлення додають нові знання про реакцію споріднених видів пшениці
на охолодження на ранніх етапах вегетації.
Ключові слова: Triticum aestivum, Triticum spelta, цитокініни, охолодження, відновлення.
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| id | nasplib_isofts_kiev_ua-123456789-202313 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1025-6415 |
| language | English |
| last_indexed | 2025-12-01T08:53:26Z |
| publishDate | 2024 |
| publisher | Видавничий дім "Академперіодика" НАН України |
| record_format | dspace |
| spelling | Voytenko, L.V. Shcherbatiuk, M.M. Vasyuk, V.A. Kosakivska, I.V. 2025-03-13T11:09:49Z 2024 Role of cytokinins in the regulation the chilling stress response in Triticum aestivum and Triticum spelta / L.V. Voytenko, M.M. Shcherbatiuk, V.A. Vasyuk, I.V. Kosakivska // Доповіді Національної академії наук України. — 2024. — № 3. — С. 69-76. — Бібліогр.: 22 назв. — англ. 1025-6415 https://nasplib.isofts.kiev.ua/handle/123456789/202313 58.036:577.175.1:582.542.1 DOI: doi.org/10.15407/dopovidi2024.03.069 The research is devoted to the study of the eff ect of chilling (+4 °C, 2 h) on cytokinin homeostasis in 14-day stressed and 21-day restored plants of winter wheat (Triticum aestivum L.) cv. “Podolyanka” and spelt wheat (Triticum spelta L.) cv. “Frankenkorn”. Our study revealed that chilling induces complex changes in the content and distribution of cytokinins in plants that are species- and organ-specifi c. Aft er chilling, the total cytokinin content in the roots of winter wheat cv. “Podolyanka” increased threefold due to the accumulation of trans-zeatin-O-glucoside, trans-zeatin, isopentenyladenine and isopentenyladenosine. Stress did not aff ect trans-zeatin riboside accumulation in roots but induced a fourfold increase in its content in the shoots of winter wheat cv. “Podolyanka”. Th e total content of cytokinin in the roots of spelt wheat cv. “Frankenkorn” decreased by 1.4 times due to decline in trans-zeatin-O-glucoside and trans-zeatin riboside content, while in shoots, it decreased by 1.2 times owing to reduced trans-zeatin-O-glucoside and isopentenyladenine levels. Prolonged exposure to chilling was manifested by an increase in hormone levels in both 21-day-old species. Our data revealed common and distinct traits in cytokinin homeostasis between winter wheat cv. “Podolyanka” and spelt wheat cv. “Frankenkorn” during rapid adaptation and the recovery period, providing new insights into the response of these species to chilling. Робота присвячена дослідженню впливу охолодження (+4 °C, 2 год) на гомеостаз цитокінінів у 14-добових стресованих та 21-добових відновлених рослин озимої пшениці (Triticum aestivum L.) сорту “Подолянка” та спельти (Triticum spelta L.) сорту “Франкенкорн”. Показано, що охолодження зумовлює комплексні зміни у вмісті і розподілі цитокінінів, які є видо- та органоспецифічними. Після охолодження загальний вміст цитокінінів (нг/г сирої речовини) у коренях пшениці зріс утричі за рахунок накопичення транс-зеатин-O- глюкозиду, транс-зеатину, ізопентеніладеніну та ізопентеніладенозину. Стрес не вплинув на накопичення транс-зеатинрибозиду у коренях, але спричинив збільшення його вмісту в чотири рази в надземній частині озимої пшениці “Подолянка”. Загальний вміст цитокінінів у коренях спельти “Франкенкорн” зменшився в 1,4 раза за рахунок зниження вмісту транс-зеатин-O-глюкозиду і транс-зеатинрибозиду, а в надземній частині — в 1,2 раза за рахунок зниження рівня транс-зеатин-O-глюкозиду та ізопентеніладеніну. Віддалений ефект охолодження виявився у підвищенні рівня цитокінінів у обох видах рослин. Визначені загальні і специфічні зміни цитокінінового гомеостазу озимої пшениці “Подолянка” та спельти “Франкенкорн” під час швидкої адаптації і в період відновлення додають нові знання про реакцію споріднених видів пшениці на охолодження на ранніх етапах вегетації. The publication contains the results of research carried out as part of the project funded by the National Academy of Sciences of Ukraine № ІІІ-90-19.463 “Hormonal regulation of growth and development of cereal plants under the influence of negative climatic factors” (2019—2023). en Видавничий дім "Академперіодика" НАН України Доповіді НАН України Біологія Role of cytokinins in the regulation the chilling stress response in Triticum aestivum and Triticum spelta Цитокініни у формуванні реакції-відповіді на охолодження в рослинах Triticum aestivum і Triticum spelta Article published earlier |
| spellingShingle | Role of cytokinins in the regulation the chilling stress response in Triticum aestivum and Triticum spelta Voytenko, L.V. Shcherbatiuk, M.M. Vasyuk, V.A. Kosakivska, I.V. Біологія |
| title | Role of cytokinins in the regulation the chilling stress response in Triticum aestivum and Triticum spelta |
| title_alt | Цитокініни у формуванні реакції-відповіді на охолодження в рослинах Triticum aestivum і Triticum spelta |
| title_full | Role of cytokinins in the regulation the chilling stress response in Triticum aestivum and Triticum spelta |
| title_fullStr | Role of cytokinins in the regulation the chilling stress response in Triticum aestivum and Triticum spelta |
| title_full_unstemmed | Role of cytokinins in the regulation the chilling stress response in Triticum aestivum and Triticum spelta |
| title_short | Role of cytokinins in the regulation the chilling stress response in Triticum aestivum and Triticum spelta |
| title_sort | role of cytokinins in the regulation the chilling stress response in triticum aestivum and triticum spelta |
| topic | Біологія |
| topic_facet | Біологія |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/202313 |
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