Surface enhanced imaging and IR spectroscopy of the biological cells on the nanostructured gold film
A new approach for optical imaging, structural study, and cell cultivation based on the effect of the enhancement of optical signals from biomolecules and biological cells near a nanostructured rough gold surface is proposed. The surface-enhanced IR absorption (SEIRA) spectroscopy and confocal micro...
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| Veröffentlicht in: | Semiconductor Physics Quantum Electronics & Optoelectronics |
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| Datum: | 2017 |
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | Englisch |
| Veröffentlicht: |
Інститут фізики напівпровідників імені В.Є. Лашкарьова НАН України
2017
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| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/214939 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Surface enhanced imaging and IR spectroscopy of the biological cells on the nanostructured gold film / G.I. Dovbeshko, O.P. Gnatyuk, S.O. Karakhim, T.P. Doroshenko, V.R. Romanyuk // Semiconductor Physics Quantum Electronics & Optoelectronics. — 2017. — Т. 20, № 2. — С. 159-167. — Бібліогр.: 28 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Zusammenfassung: | A new approach for optical imaging, structural study, and cell cultivation based on the effect of the enhancement of optical signals from biomolecules and biological cells near a nanostructured rough gold surface is proposed. The surface-enhanced IR absorption (SEIRA) spectroscopy and confocal microscopy experiments were made using the culture of SPEV (porcine embryonic kidney epithelium transplantable line) and fibroblast cells, cultivated and/or adsorbed on the gold substrate. The SEIRA spectra registered from a monolayer of the SPEV cells cultivated on the rough gold showed a low frequency shift of about 2 to 7 cm⁻¹ for the most characteristic IR vibrations, compared with those adsorbed from suspension on the same substrate. An enhancement factor of 15…30 was obtained for different molecular vibrations. The confocal microscopy contrast images of the SPEV cells on rough gold substrate were obtained in laser fluorescence mode. This approach opens new possibilities for the visualization of living cells in vivo without staining. The fluorescence of the rough gold surfaces and the effects responsible for our findings have been discussed.
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| ISSN: | 1560-8034 |