Quantitative Optical Trapping and Optical Manipulation of Micro-Sized Objects

• Published in: Semiconductor Physics Quantum Electronics & Optoelectronics
• Date: 2017
• Main Author: Sayed, Rania
• Format: Article
• Language: English
• Published: Інститут фізики напівпровідників імені В.Є. Лашкарьова НАН України 2017
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/214946
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Quantitative Optical Trapping and Optical Manipulation of Micro-Sized Objects / Rania Sayed // Semiconductor Physics Quantum Electronics & Optoelectronics. — 2017. — Т. 20, № 3. — С. 349-354. — Бібліогр.: 29 назв. — англ.

An optical tweezers technique is used for ultraprecise micromanipulation to measure positions of micrometer-scale objects with a precision down to the nanometer scale. It consists of a high-performance research microscope with a motorized scanning stage and a sensitive position detection system. Up to 10 traps can be used quasisimultaneously. Non-photodamage optical trapping of Escherichia coli (E. coli) bacteria cells of 2 µm in length, as an example of motile bacteria, has been shown in this paper. Also, efficient optical trapping and rotation of polystyrene latex particles of 3 µm in diameter have been studied, as an optical handle for the pick and place of other tiny objects. A fast galvoscanner is used to produce multiple optical traps for the manipulation of micro-sized objects, and the optical forces of these trapped objects are quantified and measured according to the explanation of the ray optics regime. The diameter of the trapped particle is bigger than the wavelength of the trapping laser light. The force constant (k) has been determined in real time from the positional time series recorded from the trapped object that is monitored by a CCD camera through a personal computer.