Settling down of Azospirillum bacteria in root zone of spring wheat under the influence of soil fungus Chaetomium cochliodes 3250
Pure bacteria cultures of Azospirillum genus able to fix atmospheric nitrogen were isolated from rhizosphere soil, washed-off roots and histosphere of spring wheat plants. By their phenotype signs and the results of molecular-genetic analysis they were related to the Azospirillum brasilense species....
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| Опубліковано в: : | Сільськогосподарська мікробіологія |
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Інститут мікробіології і вірусології ім. Д.К. Заболотного НАН України
2009
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| Цитувати: | Settling down of Azospirillum bacteria in root zone of spring wheat under the influence of soil fungus Chaetomium cochliodes 3250 / E.P. Kopilov // Сільськогосподарська мікробіологія: Міжвід. темат. наук. зб. — Чернігів, 2009. — Вип. 9. — С. 33-42. — Бібліогр.: 8 назв. — англ. |
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Kopilov, E.P. 2011-08-29T18:54:24Z 2011-08-29T18:54:24Z 2009 Settling down of Azospirillum bacteria in root zone of spring wheat under the influence of soil fungus Chaetomium cochliodes 3250 / E.P. Kopilov // Сільськогосподарська мікробіологія: Міжвід. темат. наук. зб. — Чернігів, 2009. — Вип. 9. — С. 33-42. — Бібліогр.: 8 назв. — англ. 1997-3004 https://nasplib.isofts.kiev.ua/handle/123456789/26195 579.64:631.461.52 Pure bacteria cultures of Azospirillum genus able to fix atmospheric nitrogen were isolated from rhizosphere soil, washed-off roots and histosphere of spring wheat plants. By their phenotype signs and the results of molecular-genetic analysis they were related to the Azospirillum brasilense species. It was shown that strain A. brasilensе 104 actively colonizes rhizospheric soil, root surface and is able to penetrate in inner plant tissues during its introduction in root system of spring wheat. The soil ascomycete Chaetomium cochliodes 3250 promotes settling down of Azospirillum bacteria in root zone of spring wheat especially in plants hystosphere which results in increasing of chlorophyll a and b content in leaves. Из ризосферной почвы, отмытых корней и гистосферы яровой пшеницы выделены активные штаммы бактерий рода Azospirillum, способные к фиксации атмосферного азота. Изучение фенотипических признаков и проведение молекулярно-генетичес-кого анализа позволило отнести их к Azospirillum brasilense. Показано, что штамм A. brasilensе 104 при интродукции в корневую систему яровой пшеницы активно колонизирует ризосферную почву, поверхность корней и способен проникать во внутренние ткани растений. Почвенный сумчатый гриб Chaetomium cochliodes 3250 способствует лучшей приживаемости азоспирилл в корневой зоне яровой пшеницы, особенно в гистосфере растений, что отражается на увеличении содержания хлорофиллов a и b в листьях культуры. Із ризосферного ґрунту, відмитих кооренів та гістосфери пшениці ярої виділені активні штами бактерій роду Azospirillum, здатні фіксувати атмосферний азот. Вивчення ознак фенотипу і проведення молекулярно-генетичного аналізу дозволило віднести їх до Azospirillum brasilense. Показано, що штам A. brasilensе 104 при інтродукції в кореневу систему пшениці ярої активно колонізує ризосферний ґрунт, поверхню коренів і здатен проникати у внутрішні тканини рослин. Ґрунтовий сумчатий гриб C. cochliodes 3250 сприяє кращій приживаності азоспірил в кореневій зоні пшениці ярої, особливо у гістосфері рослин, що позначається на збільшенні вмісту хлорофілів a і b в листках культури. en Інститут мікробіології і вірусології ім. Д.К. Заболотного НАН України Сільськогосподарська мікробіологія Загальна і ґрунтова мікробіологія Settling down of Azospirillum bacteria in root zone of spring wheat under the influence of soil fungus Chaetomium cochliodes 3250 Приживаемость азоспирилл в корневой зоне яровой пшеницы под влиянием почвенного гриба Chaetomium cochliodes 3250 Приживаність азоспірил у кореневій зоні пшениці ярої під впливом грунтового гриба Chaetomium cochliodes 3250 Article published earlier |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| title |
Settling down of Azospirillum bacteria in root zone of spring wheat under the influence of soil fungus Chaetomium cochliodes 3250 |
| spellingShingle |
Settling down of Azospirillum bacteria in root zone of spring wheat under the influence of soil fungus Chaetomium cochliodes 3250 Kopilov, E.P. Загальна і ґрунтова мікробіологія |
| title_short |
Settling down of Azospirillum bacteria in root zone of spring wheat under the influence of soil fungus Chaetomium cochliodes 3250 |
| title_full |
Settling down of Azospirillum bacteria in root zone of spring wheat under the influence of soil fungus Chaetomium cochliodes 3250 |
| title_fullStr |
Settling down of Azospirillum bacteria in root zone of spring wheat under the influence of soil fungus Chaetomium cochliodes 3250 |
| title_full_unstemmed |
Settling down of Azospirillum bacteria in root zone of spring wheat under the influence of soil fungus Chaetomium cochliodes 3250 |
| title_sort |
settling down of azospirillum bacteria in root zone of spring wheat under the influence of soil fungus chaetomium cochliodes 3250 |
| author |
Kopilov, E.P. |
| author_facet |
Kopilov, E.P. |
| topic |
Загальна і ґрунтова мікробіологія |
| topic_facet |
Загальна і ґрунтова мікробіологія |
| publishDate |
2009 |
| language |
English |
| container_title |
Сільськогосподарська мікробіологія |
| publisher |
Інститут мікробіології і вірусології ім. Д.К. Заболотного НАН України |
| format |
Article |
| title_alt |
Приживаемость азоспирилл в корневой зоне яровой пшеницы под влиянием почвенного гриба Chaetomium cochliodes 3250 Приживаність азоспірил у кореневій зоні пшениці ярої під впливом грунтового гриба Chaetomium cochliodes 3250 |
| description |
Pure bacteria cultures of Azospirillum genus able to fix atmospheric nitrogen were isolated from rhizosphere soil, washed-off roots and histosphere of spring wheat plants. By their phenotype signs and the results of molecular-genetic analysis they were related to the Azospirillum brasilense species. It was shown that strain A. brasilensе 104 actively colonizes rhizospheric soil, root surface and is able to penetrate in inner plant tissues during its introduction in root system of spring wheat. The soil ascomycete Chaetomium cochliodes 3250 promotes settling down of Azospirillum bacteria in root zone of spring wheat especially in plants hystosphere which results in increasing of chlorophyll a and b content in leaves.
Из ризосферной почвы, отмытых корней и гистосферы яровой пшеницы выделены активные штаммы бактерий рода Azospirillum, способные к фиксации атмосферного азота. Изучение фенотипических признаков и проведение молекулярно-генетичес-кого анализа позволило отнести их к Azospirillum brasilense. Показано, что штамм A. brasilensе 104 при интродукции в корневую систему яровой пшеницы активно колонизирует ризосферную почву, поверхность корней и способен проникать во внутренние ткани растений. Почвенный сумчатый гриб Chaetomium cochliodes 3250 способствует лучшей приживаемости азоспирилл в корневой зоне яровой пшеницы, особенно в гистосфере растений, что отражается на увеличении содержания хлорофиллов a и b в листьях культуры.
Із ризосферного ґрунту, відмитих кооренів та гістосфери пшениці ярої виділені активні штами бактерій роду Azospirillum, здатні фіксувати атмосферний азот. Вивчення ознак фенотипу і проведення молекулярно-генетичного аналізу дозволило віднести їх до Azospirillum brasilense. Показано, що штам A. brasilensе 104 при інтродукції в кореневу систему пшениці ярої активно колонізує ризосферний ґрунт, поверхню коренів і здатен проникати у внутрішні тканини рослин. Ґрунтовий сумчатий гриб C. cochliodes 3250 сприяє кращій приживаності азоспірил в кореневій зоні пшениці ярої, особливо у гістосфері рослин, що позначається на збільшенні вмісту хлорофілів a і b в листках культури.
|
| issn |
1997-3004 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/26195 |
| citation_txt |
Settling down of Azospirillum bacteria in root zone of spring wheat under the influence of soil fungus Chaetomium cochliodes 3250 / E.P. Kopilov // Сільськогосподарська мікробіологія: Міжвід. темат. наук. зб. — Чернігів, 2009. — Вип. 9. — С. 33-42. — Бібліогр.: 8 назв. — англ. |
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2025-11-26T00:09:30Z |
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| fulltext |
33
�� 579.�64:631.�461.�52
SETTLING DOWN OF Azospirillum BACTERIA
IN ROOT ZONE OF SPRING WHEAT UNDER THE
INFLUENCE OF SOIL FUNGUS Chaetomium
CoChliodes 3250
Kopilov E�P�
Institute of Agriculture �icrobiology UAAS
97, Shevchenko str.�, �hernihiv, 14027, Ukraine, Shevchenko str.�, �hernihiv, 14027, UkraineShevchenko str.�, �hernihiv, 14027, Ukrainestr.�, �hernihiv, 14027, Ukraine.�, �hernihiv, 14027, Ukraine, Ukraine
E-mail: evhenykopilov@rambler.�ru
Pure bacteria cultures of Azospirillum genus able to fix
atmospheric nitrogen were isolated from rhizosphere soil, washed-off
roots and histosphere of spring wheat plants. By their phenotype signs
and the results of molecular-genetic analysis they were related to the
Azospirillum brasilense species. It was shown that strain A. brasilensе
104 actively colonizes rhizospheric soil, root surface and is able to
penetrate in inner plant tissues during its introduction in root system
of spring wheat. The soil ascomycete Chaetomium cochliodes 3250
promotes settling down of Azospirillum bacteria in root zone of spring
wheat especially in plants hystosphere which results in increasing of
chlorophyll a and b content in leaves.
Key words: Azospirillum brasilense, nitrogen-fixing bacteria,
spring wheat, endophytes, chlorophyll, Chaetomium cochliodes 3250
�acteria of Azospirillum genus are the one of the most investigated
ob�ects among plants rhizosphere.� The manner of their influence on plants
is multifaceted.� This is a high N2-fixing activity and as a consequence
proving of nitrogen plants nutrition, capability to produce biological
active substances which activate chloroplast genesis, stimulate plants
growth and development, rise up their resistant to unfavorable conditions
of environment and deceases agents.�
The aim of this work was studying of Chaetomium cochliodes
Palliser 3250 peculiarities to be conductive to Azospirillum bacteria to
penetrate into spring wheat roots.�
materials and methods. �alculation of Azospirillum bacteria we
made on �aceras solid agar medium with red congo where they make
dark red, little, dry, wrinkled colonies 0,5-1,5 mm in diameter [1].�
Preliminary identification of selected bacteria was carried out by
morphological, cultural, physiological and biochemical features accord-
34
ing to �ergi key [8].� �esides we also used the description of bacteria
from Azospirillum genus in original publications [4].�
We used universal oligonucleotide primers which was conformed
to conservative positions on 3-’ and 5-’ endings of 16S rRNA gene.�
16S Forward (�GG-���-AGA-�T�-�TA-�GG-GAG-G�A-G�A)
and 16S Revers (G�G-TGG-A�T-A��-AGG-GTA-T�T-AAT-��).�
The reaction of amplification was carried out on Applied �iosystems
appliance.� P�R-products were separated in 1,5 % agarose by horizontal
electrophoresis.� Detection of obtained P�R-products was made on
Applied �iosystems A�I Prism 3130 automatic capillary analizer.�
The ability of C. cochliodes 3250 to colonize spring wheat root
system was investigated in laboratory experiment with sterile conditions
[7].� �olouring of mold mycelium in root tissues made by Kobel method
[6].� �icropreparations were investigated under microscope МС 200(Т)
(“�icros”, Austria).� Photos were made by color digital camera Е 5400
(“Nikon”, Korea).�
The maintenance of chlorophylls a and b in leafs was investigated
by spectrophotometer method [5].�
results and discussions. From rhizosphere soil, washed off roots
and histosphere of spring wheat we picked out clean bacteria cultures
of Azospirillum genus.� There were selected the 11 most active strains
able to fix atmospheric nitrogen.� They were described by phenotypic
features and investigated with molecular-genetic analysis methods.� For
the present day Gen�ank database contains information about 11 species
of Azospirillum genus bacteria.� As is well known from the literature
sources all the Azospirillum strains selected from Ukrainian soils belong
to A. brasilense and A. lipoferum.� Therefore we carried through the
phylogenetic analysis of picked up strains as well as typical strains of
A. brasilense Sp7 and A. lipoferum 59b obtained from the �acterial
�ollection of the Institute of �iochemistry and Physiology of Plants and
�icroorganisms RAS.�
�n the basic of cultural, morphological, physiological and
biochemical signs the selected strains were considered to Azospirillum
genus, A. brasilense species.�
With the help of P�R-analysis the amplificants of 16S rRNA gene
were obtained and sequenced.� Electrophoresis of P�R-products in 1,5 %
agarose is presented on the fig.� 1
35
Fig. 1. Electrophoresis of PCR-products in 1,5 % agarose
The results of investigated strains identification obtained during
comparative analysis of nucleotide sequences of the bacterial 16S rRNA
genuse with similar sequences of A. brasilense and A. lipoferum from
Gen�ank database are presented in the table 1.�
The comparative analysis of sequencing results revealed 100 %
identity of 16S rRNA of investigated bacteria with the similar sequences
of A. brasilense Gen�ank database.� Thus the identification of the selected
strains with the help of sequence analysis of 16S rRNA gene matches
with the results obtained during investigation of phenotypic signs.�
The selected Azospirillum strains were tested in series of vegetative
experiments with spring wheat, where their ability to form associations
with plants rising up N2-fixing activity in root zone, activating synthesis
of photosynthetic pigments, proving plants growth and development
have been studied.� As a result we found out the most effective strain
A. brasilensе 104.�
�ne of the important conditions of the effective interaction of
introduced associative bacteria strains with plants is the capability of
microorganisms to colonize plants roots actively.� For investigation of
the ability of A. brasilensе 104 to settle down in spring wheat root zone
we obtained streptomycin stable mutant which didn’t differ from primer
strain by its cultural, morphological and physiological properties.�
We investigated the dynamics of quantity of mutant cells
introduced into root zone of spring wheat in rhizospheric soil, on the
washed off roots and hystosphere of plants in vegetative experiment.�
The obtained results testify that Azospirillum cells are able to settle
down not only in rhizosphere soil and root surface but they penetrate
36
into inner tissues of spring wheat plants.�
Table 1. the results of identification of investigated strains
during comparative analysis of nucleotide sequences of
16s-RNa gene from Gen Bank database
Strain
number Species and the number in Gen �ank Identity,
%
The number
of nucleoti-
des identical
to 16S RNA
fragment
1 2 3 4
145 Azospirillum brasilense DQ438999
Azospirillum sp.� 7� AF411852 100 392 (r)
121 A. brasilense DQ438999
Azospirillum sp.� 7� AF411852 100 394 (r)
114 A. brasilense DQ438999
Azospirillum sp.� 7� AF411852 100 395 (r)
108 A. brasilense DQ438999
Azospirillum sp.� 7� AF411852 100 382 (r)
93 A. brasilense brasilensebrasilense DQ438999
Azospirillum sp.� 7� AF411852 100 373 (r)
89
A. brasilense DQ438999
Azospirillum sp.� 7� AF411852
A. brasilense A�324110
A. brasilense (N�I�� 11860) Z29617
100 388 (r)
102
A. brasilense DQ438999
Azospirillum sp.� 7� AF411852
A. brasilense A�324110
A. brasilense (N�I�� 11860) Z29617
100 389 (r)
85
A. brasilense DQ438999
Azospirillum sp.� 7� AF411852
A. brasilense A�324110
A. brasilense (N�I�� 11860) Z29617
100 395 (r)
83
A. brasilense DQ438999
Azospirillum sp.� 7� AF411852
A. brasilense A�324110
A. brasilense (N�I�� 11860) Z29617
100 395 (r)
72
A. brasilense DQ438999
Azospirillum sp.� 7� AF411852
A. brasilense A�324110
A. brasilense (N�I�� 11860) Z29617
100 373 (r)
37
1 2 3 4
67
A. brasilense DQ438999
Azospirillum sp.� 7� AF411852
A. brasilense A�324110
A. brasilense (N�I�� 11860) Z29617
100 361 (r)
A. brasilense
Sp.� 7
A. brasilense DQ438999
Azospirillum sp.� 7� AF411852
A. brasilense A�324110
A. brasilense (N�I�� 11860) Z29617
100 366 (r)
A. lipoferum
59b
A. lipoferum strain I��P EF100150
A. lipoferum (ncimb 11861) Z29619 100 355 (r)
Note: (r) is a reverse primer used for amplification of 16S rRNA gene at
the investigated strains
Endophytic property is very useful for micro- and macrosymbionts.�
Plats infected with endophyts own their growth processes activated,
heightened immune status and formed resistance to stress factors of
environment.� Localization of endophyts inside the plant tissues gives
them advantages in comparison with rhizosphere microorganisms
according to nutritious substances access and the absent of competition
with aboriginal microflora.� As well, endophyts which penetrate in inner
tissues, found themselves in more comfortable conditions because of
they are protected from negative influence of environment.� N2-fixing
endophyts, particularly Azospirillum, arouse interest because of their
ecological role and possible practical application.� Localization of
Azospirillum bacteria in plant tissues is favourable for N2-fixing because
in microzones of their localization ensured low partial oxygen pressure
which is necessary for active functioning of bacterial nitrogenase and
contains accessible material for this process.�
�esides, the close interaction realizes between micro- and
macropartner which contributes to exchange with signal molecules and
metabolites.� Particularly, N2 and phytohormones come in into plants
without any loss.�
However there are not enough understood and investigated the
questions of bacteria penetrating into plant tissues.� It considers that
Azospirillum bacteria don’t have enough pectolytic and proteolytic
activity for that [2].� At the same time during thoroughly checked
experimental conditions it was achieved that under the influence of
host-plant the incensement of pectolytic activity became 7-8 times more
38
and the incensement of proteolytic activity became 10-11 times more.�
According to fuller information some mycorrhizal fungi can favour
Azospirillum bacteria to penerate into plants [3].�
At the Institute of Agriculture �icrobiology UAAS the strain of
soil ascomycete Chaetomium cochliodes Palliser 3250, which manifests
high antagonistic activity against culture plants root rot agents was
singled out.� We revealed that foil fungus C. cochliodes Palliser 3250
actively colonizes the root system of spring wheat forming carposomes
on the root surface, penetrating into the root hairs and rhizoderma cells.�
At the same time the level of micro- and macroelements absorption gets
higher, rises up the maintenance of photosynthetic pigments, proves the
growth and development of plants.� Thus, C. cochliodes 3250 is able to
form endophytic association with spring wheat roots.�
For investigation the ability of C. cochliodes 3250 to further to
penetrate in hystosphere of spring wheat of Azospirillum bacteria in
vegetative experimtnt we examined the quantity dynamics of mutant
introduced in spring wheat root system together with C. cochliodes
3250 (fig.� 2.�)
Fig. 2. Colonization of spring wheat root sphere with
Azospirillum spstr
The obtained results testify that in rhizosphere soil observed little
lowering and further stabilization of introduced mutant quantity on the
level of 1,6-4,0 Ч 105 bacterial cells in 1 g of soil.� C. cochliodes 3250
was conductive to increasing the quantity of Azospirillum cells up to 3,6-
6,4 Ч 106.� We explain such fact on the basic of high antagonistic activity
of C. cochliodes 3250 according to phytopathogenic fungi which can
0
1
2
3
4
5
6
7
8
7 14 21 28 35 42
days after introduction
lg
o
f q
ua
nt
ity
, c
el
ls
/g
o
f d
ry
ro
ot
s(
so
il)
Root surface
Rhizosphere
Hystosphere
39
provoke root rots, and as we revealed earlier, during its introduction
in chernozem and podzol soils takes place considerable limitation of
phytopathogenic fungi from Fusarium genus.� Limiting the development
of phytopathogenic fungi C. cochliodes 3250 furthers to settling down
of Azospirillum bacteria in rhizospheric soil.� It is necessary to take
into account the fact that for Azospirillum cells development the most
favourable are the microaerobic conditions which can be made by
C. cochliodes 3250 during lowering �2 containing in N2-fixing zone.�
The results reveal that on the surface of spring wheat roots
Azospirillum bacteria settled down more actively and their quantity
was on one order more than in rhizospheric soil.� The influence of
C. cochliodes 3250 in this case was less aloud.�
The quantity of Azospirillum bacteria in spring wheat inner tissues
was greatly less and amounted 1,0-2,5 × 102 bacterial cells in 1g of roots.�
Under the influence of C. cochliodes 3250 the ability of Azospirillum
bacteria to penetrate into the roots became much more (on 3-4 order)
and in hystosphere of spring wheat counted 4,0 × 105-2,5 × 106 bacterial
cells in 1 g of roots (table 2).�
Table 2. Colonization of different spring wheat root spheres
with a. brasilense 102str (vegetative experiment)
The variants of experiment
The number of bacterial cells
piece/g absolutely dry roots (soil)
days after introduction
7 14 21
Rhizospheric soil
inoculation with
A. brasilense 102str 3,1 × 106 1,0 × 105 3,2 × 105
inoculation with
A. brasilense 102str +
C. cochliodes 3250
5,6 × 106 1,3 × 106 5,6 × 106
Washed off roots
inoculation with
A. brasilense 102str 3,1 × 107 6,3 × 106 2,5 × 106
inoculation with
A. brasilense 102str +
C. cochliodes 3250
2,0 × 107 7,1 × 106 4,0 × 106
Hystosphere
inoculation with
A. brasilense 102str 0,12 × 102 0,32 × 102 1,26 × 102
inoculation with
A. brasilense 102str +
C. cochliodes 3250
4,5 × 105 4,0 × 105 2,5 × 106
40
Thus it is possible to make a conclusion that forming endophyt
association with spring wheat roots C. cochliodes 3250 furthers to settle
down of Azospirillum bacteria in rhizospheric soil and hystosphere of
spring wheat.�
�omplex inoculation of spring wheat with A. brasilensе 104str
and C. cochliodes 3250 told upon chloroplast genesis of the culture
(table 3).� The plants during it were characterized by high maintenance
of chlorophylls a and b in leafs.�
Table 3. the influence of spring wheat inoculation with mi-
croorganisms on chlorophyll a and b maintenance in leafs of
plants (vegetative experiment)
The variants of experiment
�hlorophyll concentration, mg/100 g
of leafs
a b a+b
Without inoculation (control) 42,56 13,86 56,42
Inoculation with
A. brasilense 104str 52,87 17,95 70,85
Inoculation of seeds with
A. brasilense 104 str +
C. cochliodes 3250
68,85 23,84 92,69
The less essential difference 05 0,80 1,23
Thus, the strain of A. brasilensе 104 during its introduction in
root system of spring wheat actively colonizes rhizospheric soil, root
surface and is able to penetrate in inner plant tissues.� The soil ascomucete
C. cochliodes 3250 furthers for better settling down of Azospirillum cells
in spring wheat root zone especially in plants hystosphere which has an
effect on increasing of chlorophyll a and b in the leafs of the culture.�
1.� �aceras S.�A.�R.� Improved medium for isolation of Azospirillum spp.�
/�aceras S.�A.�R.� //Appl.� Environ.� �icrobiol.� – 1982.� – Vol.� 44, № 4.� – P.� 990-
991.�
2.� �kon �.� Development and function of Azospirillum- inoculated roots
/�kon �.�, Kapulnik �.� //Plant and Soil.� – 1986.� – Vol.� 90, N 1.� − P.� 3-16.�
3.� Paula �.�A.� Synergistic effects of vesicular-arbuscular mycorrhizal
fungi and diazotrophic bacteria on nutrition and growth of sweet potato
(Ipomoea batatas) /Paula �.�A.�, Urquiaga S.�, Siqueira J.��.�, Dodereiner J.�
//�iol.� Fertil.� Soils.� – 1992.� – Vol.� 14.� – P.� 61-66.�
4.� Tarrand J.�J.� A taxonomic study of the Spirillum lipoferum group,
41
with descriptions of a new genus, Azospirillum gen.� nov.� and two species,
Azospirillum lipoferum (�ei�erinck) comb. nov.� and Azospirillum brasilense
sp. nov.� /Tarrand J.�J.�, Krieg N.�R.�, Dobereiner J.� //�an.� J.� �icrobiol.� – 1978.�
– Vol.� 24.� – P.� 967-980.�
5.� Гродзинский А.�М.� �раткий справочник по физиологии растений
/А.�М.� Гродзинский, �.�М.� Гродзинский.� – �.�: Наук.� думка, 1973.� – 567 с.�
6.� Методы �кспериментальной микологии: Справочник /Под ред.�
В.�И.� Билай.� – �.�: Наук.� думка, 1982.� – 549 с.�
7.� Мирчинк Т.�Г.� Почвенная микология /Мирчинк Т.�Г.� – М.�: Изд.�
МГУ, 1976.� – 206 с.�
8.� Определитель бактерий Берджи /Под ред.� �ж.� Хоулта, Н.� �рига,
П.� Снита, �ж.� Стейнли, С.� Уилльямса: Пер.� с англ.� под ред.� Г.�А.� �аварзина.�
– М.�: Мир, 1997.� – 800 с.�
ПРИЖИВАЕМОСТЬ АЗОСПИРИЛЛ В КОРНЕВОЙ
ЗОНЕ ЯРОВОЙ ПШЕНИЦЫ ПОД ВЛИЯНИЕМ
ПОЧВЕННОГО ГРИБА Chaetomium CoChliodes
3250
Копылов Е�П�
Институт сельскохозяйственной микробиологии УААН,
г.� Чернигов
Из ризосферной почвы, отмытых корней и гистосферы
яровой пшеницы выделены активные штаммы бактерий рода
Azospirillum, способные к фиксации атмосферного азота. Изучение
фенотипических признаков и проведение молекулярно-генетичес-
кого анализа позволило отнести их к Azospirillum brasilense.
Показано, что штамм A. brasilensе 104 при интродукции в корневую
систему яровой пшеницы активно колонизирует ризосферную
почву, поверхность корней и способен проникать во внутренние
ткани растений. Почвенный сумчатый гриб Chaetomium cochliodes
3250 способствует лучшей приживаемости азоспирилл в корневой
зоне яровой пшеницы, особенно в гистосфере растений, что
отражается на увеличении содержания хлорофиллов a и b в
листьях культуры.
�лючевые слова: Azospirillum brasilense, диазотрофы, яровая
пшеница, эндофиты, хлорофилл, Chaetomium cochliodes 3250.
42
ПРИЖИВАНІСТЬ АЗОСПІРИЛ У КОРЕНЕВІЙ ЗОНІ
ПШЕНИЦІ ЯРОЇ ПІД ВПЛИВОМ ГРУНТОВОГО
ГРИБА Chaetomium CoChliodes 3250
Копилов Є�П�
Інститут сільськогосподарської мікробіології УААН, м.� Чернігів
Із ризосферного ґрунту, відмитих кооренів та гістосфери
пшениці ярої виділені активні штами бактерій роду Azospirillum,
здатні фіксувати атмосферний азот. Вивчення ознак фенотипу і
проведення молекулярно-генетичного аналізу дозволило віднести
їх до Azospirillum brasilense. Показано, що штам A. brasilensе 104
при інтродукції в кореневу систему пшениці ярої активно колонізує
ризосферний ґрунт, поверхню коренів і здатен проникати у
внутрішні тканини рослин. Ґрунтовий сумчатий гриб C. cochliodes
3250 сприяє кращій приживаності азоспірил в кореневій зоні
пшениці ярої, особливо у гістосфері рослин, що позначається на
збільшенні вмісту хлорофілів a і b в листках культури.
�лючові слова: Azospirillum brasilense, діазотрофи, пшениця
яра, ендофіти, хлорофіл, Chaetomium cochliodes 3250.
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