Synthesis and Investigation of Modified Silica Coatings for Biotechnology
Mesoporous organic-inorganic hybrid composites on glass substrates were prepared by the sol-gel method for testing the proteins adhesion. Different types of hydrophobic/hydrophilic silica sol-gels were prepared using tetraethoxysilane (TEOS) as starting material and modified with hexamethyldisilazan...
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Інститут хімії поверхні ім. О.О. Чуйка НАН України
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| Cite this: | Synthesis and Investigation of Modified Silica Coatings for Biotechnology / V. Tomkute, A. Beganskiene, A. Kareiva, S. Zemljic Jokhadar, U Batista // Хімія, фізика та технологія поверхні. — 2010. — Т. 1, № 3. — С. 348-354. — Бібліогр.: 19 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1859811540255899648 |
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| author | Tomkute, V. Beganskiene, A. Kareiva, A. Zemljic Jokhadar, S. Batista, U. |
| author_facet | Tomkute, V. Beganskiene, A. Kareiva, A. Zemljic Jokhadar, S. Batista, U. |
| citation_txt | Synthesis and Investigation of Modified Silica Coatings for Biotechnology / V. Tomkute, A. Beganskiene, A. Kareiva, S. Zemljic Jokhadar, U Batista // Хімія, фізика та технологія поверхні. — 2010. — Т. 1, № 3. — С. 348-354. — Бібліогр.: 19 назв. — англ. |
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| description | Mesoporous organic-inorganic hybrid composites on glass substrates were prepared by the sol-gel method for testing the proteins adhesion. Different types of hydrophobic/hydrophilic silica sol-gels were prepared using tetraethoxysilane (TEOS) as starting material and modified with hexamethyldisilazane (HMDS). Sol-gel thin films were successfully prepared with the dip-coating technique on glass surfaces. The coatings surface characteristics were evaluated. The prepared sol-gel derived colloidal silica coatings and modified coatings were characterized by wettability measurements. Also, infrared spectroscopy, atomic force microscope (AFM) assay were used to characterise the surfaces. The coatings of colloidal silica (VT104, water contact angle 17°), polysiloxane sol (VT111, 64°) methyl-modified sols (VT079, 144° and VT112, 47°) with various wettability properties were tested for CaCo-2 cells proliferation. Methylmodified coating VT112 proved to be the best substrate for cell proliferation.
Для тестування адгезії білків золь-гель методом приготовано мезопоруваті органо-неорганічні гібридні композити на скляних підкладинках. Різні типи гідрофобних та гідрофільних кремнеземних золів та гелів було приготовано з використанням тетраетоксисилану (ТЕОС) як вихідного матеріалу і модифіковано гексаметилдисилазаном (ГМДС). Тонкі золь-гель плівки було успішно приготовано за допомогою процедури глазурування зануренням на скляних поверхнях. Одержано характеристики поверхонь покриттів. Одержані шляхом золь-гель синтезу покриття – похідні колоїдного кремнезему та модифіковані покриття було охарактеризовано вимірюванням змочуваності. Для характеризації поверхонь було також використано аналіз за допомогою інфрачервоної спектроскопії та атомної силової мікроскопії (АСМ). Покриття з колоїдного кремнезему (VT104, кут змочування водою 17°), полісилоксановий золь (VT111, 64°), метил-модифіковані золі (VT079, 144° та VT112, 47°) з різною змочуваністю було протестовано для розмноження клітин СаСо-2. Метил-модифіковане покриття VT112 виявилось найкращим субстратом для розмноження клітин.
Для тестирования адгезии белков золь-гель методом приготовлены мезопористые органо-неорганические гибридные композиты на стеклянных подложках. Разные типы гидрофобных и гидрофильных кремнеземных золей и гелей были приготовлены с использованием тетраэтоксисилана (ТЭОС) как исходного материала и модифицированы гексаметилдисилазаном (ГМДС). Тонкие золь-гель пленки были успешно приготовлены с помощью процедуры глазурирования погружением на стеклянных поверхностях. Получены характеристики поверхностей покрытий. Полученные путем золь-гель синтеза покрытия – производные коллоидного кремнезема и модифицированные покрытия были охарактеризованы измерением смачиваемости. Для характеризации поверхностей был также использован анализ с помощью инфракрасной спектроскопии и атомной силовой микроскопии (АСМ). Покрытия из коллоидного кремнезема (VT104, угол смачивания водой 17°), полисилоксановый золь (VT111, 64°), метил-модифицированные золи (VT079, 144° и VT112, 47°) с разной смачиваемостью были протестированы для размножения клеток СаСо-2. Метилмодифицированное покрытие VT112 оказалось наилучшим субстратом для размножения клеток.
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Хімія, фізика та технологія поверхні. 2010. Т. 1. № 3. С. 348–354
_____________________________________________________________________________________________
348 ХФТП 2010. Т. 1. № 3
UDC 544.723
SYNTHESIS AND INVESTIGATION OF MODIFIED SILICA
COATINGS FOR BIOTECHNOLOGY
V. Tomkute1, A. Beganskiene1, A. Kareiva1, S. Zemljic Jokhadar2, U Batista2
1Department of General and Inorganic Chemistry, Vilnius University
24 Naugarduko Street, Vilnius Lt-3225, Lithuania
2Faculty of Medicine, Institute of Biophysics
2 Lipiceva Street, Ljubljana SI-1000, Slovenia
Mesoporous organic-inorganic hybrid composites on glass substrates were prepared by the sol-gel
method for testing the proteins adhesion. Different types of hydrophobic/hydrophilic silica sol-gels were
prepared using tetraethoxysilane (TEOS) as starting material and modified with hexamethyldisilazane
(HMDS). Sol-gel thin films were successfully prepared with the dip-coating technique on glass surfaces.
The coatings surface characteristics were evaluated. The prepared sol-gel derived colloidal silica coat-
ings and modified coatings were characterized by wettability measurements. Also, infrared spectroscopy,
atomic force microscope (AFM) assay were used to characterise the surfaces. The coatings of colloidal
silica (VT104, water contact angle 17°), polysiloxane sol (VT111, 64°) methyl-modified sols (VT079, 144°
and VT112, 47°) with various wettability properties were tested for CaCo-2 cells proliferation. Methyl-
modified coating VT112 proved to be the best substrate for cell proliferation.
INTRODUCTION
A combination of sol-gel compounds and
biomaterials like bio-molecules, cell, bacteria,
viruses and etc. resulting in added functionality.
Bio-doped hybrid materials provide a unique op-
portunity for physicists, chemists, biologists and
material scientists to mould the new area of nano-
biotechnology [1, 2]. Organic and biological ma-
terials combination with the sol-gel matrices
could be used for various applications: in bio-
molecular electronics, biosensors, bio-actuators
and medicines, namely in photodynamic antican-
cer therapy, targeted delivery of radio isotopes,
drug delivery, electronic DNA sequencing,
nanotechnology of gene delivery system and gene
therapy [1–7].
Bio-molecules are naturally occurring mole-
cules in living organisms, e.g., amino acids and
nucleotides, consisting primarily of carbon, hy-
drogen, oxygen, nitrogen, phosphorus, and sulfur.
Various biological molecules adhesion and
growth on the sol-gel matrix is influenced by bio-
active surface properties as type and the density
of surface charge, balance between the hydro-
philicity and the hydrophobicity on surface, the
chemical structure and functional groups, surface
topography and roughness, the interfacial free
energy, etc. [6]. The surface structure must be
similar to the biological molecules properties,
consequently inorganic surfaces are modifiable
with substrates which enhances interaction: or-
ganic materials, proteins, antibodies, antigens,
polymers, etc. [1–7]. Thus, the essential require-
ments for the formation of surface structures are
the possibility to modify, reiteration of the results,
method must be simple and low-cost. The sol-gel
derived inorganic matrices (films, micro-spheres
or fibers) offer several advantages compared with
organic polymers such as mechanical strength,
non-toxicity or chemical inertness, so they may
be used in applications where biocompatibility
and/or thermal stability requirements are essen-
tial. During this method is available to use or-
ganic and inorganic compounds, it is possible to
synthesize a various substances: powders, thick,
thin or multilayer films, ceramic structures,
nanoporous organic-inorganic membrane materi-
als, etc. [8–14]. Moreover, the sol-gel matrix with
the large surface area, porosity provides the ad-
vantages of optical transparency, good compati-
bility and large immobilization capacity. Sol-gel
entrapment method not only improves the resis-
tance of bio-molecules to thermal and chemical
denaturation but also increases the storage stabil-
ity. In the past few years, numerous silica- and/or
Synthesis and Investigation of Modified Silica Coatings for Biotechnology
_____________________________________________________________________________________________
ХФТП 2010. Т. 1. № 3 349
porous siloxane based organic–inorganic materi-
als have been employed to immobilize or capsu-
late proteins, enzymes, polysaccharides, nucleic
acids, phospholipids, as well as the cells hybrid
materials [1–6, 15]. Hybrid sol-gel films were
developed for tissue-derived cell growth [7]. It
was found that immobilized biological materials
trapped within sol-gel glasses usually retain their
catalytic activity and can even be protected
against degradation [4, 5, 7, 8, 10, 11]. Bioactive
coating is also applicable for detection of viruses
and for the generation of anti-viral vaccines [4].
This report presents investigations of the
formation, modification and characterization of
different silica-based mesoporous organic–
inorganic hybrid materials on glass substrates
which could useful for the adhesion or growth of
bio-molecules have done. Different amount of
methyl groups were introduced onto the colloidal
silica to get surfaces with miscellaneous proper-
ties. In further investigations, modified and pro-
tein coated sol-gel surface with different wettabil-
ity properties have been tested for CaCo-2 cell
proliferation.
EXPERIMENTAL
Preparation of Sols. The precursor of SiO2
(A sol) colloidal sol was prepared by the base
catalyzed hydrolysis of tetraethylorthosilicate
TEOS (TEOS, C8H20O4Si, ≥98%, Fluka) by the
following method of preparation of Stöber silica
[16]. The ammonia solution in ethanol was added
to the solution of TEOS in ethanol with continu-
ous stirring at room temperature 20±2oC
(TEOS:NH3:H2O:EtOH molar ratio 1:0.2:2.37:38,
respectively). The molar ratio of ammonium hy-
droxide to alkoxide was 0.2 mol, to water –
0.4 mol. The solution with final silica concentra-
tion of 3% was prepared. The obtained reaction
mixture was stored for 4 days at room tempera-
ture to allow hydrolysis as much as possible. The
final product consisted of colloidal suspension of
SiO2 nanoparticles in an anhydrous solvent. Poly-
siloxane (PS) 3% (B sol) sol was obtained using
acid hydrolysis of TEOS (TEOS:HCl:H2O:EtOH
molar ratio 1:0.01:4:37.4) in ethanol.
Methyl-modified SiO2 sols were prepared by
adding different amount of hexamethyldisilazane
(HMDS, C9H19NSi2, 98 %, Aldrich) to the 3%
colloidal silica suspension. HMDS modified sols
were aged 2 days (SiO2:HMDS:EtOH molar ra-
tios: C1 sol 1:0.16:44.05 (0,625 % HMDS); C2
sol 1:0.32:44.59 (1.25 % HMDS); C3 sol 1:2.5:45
(10 % HMDS)) at room temperature.
Preparation of Coatings. Coatings were pre-
pared on glass (Menzel-Glaser, 76x26 mm) sub-
strates previously cleaned and dried. Dip-coating
method on both sides of the glass was employed
to produce sol-gel coatings using apparatus KSV
Instruments Ltd. KSV D™. The parameters of
dipping were as following: immersion rate –
40 mm/min and dipping time – 20 s.
Characterization of Sols and Coatings. IR
spectra of the materials were recorded using ATR
Perkin-Elmer Spectrum BX FT-IR spectrometer.
The AFM images of the silica coatings on glass
were performed on Multimode Scanning Probe
Microscope (Digital Instruments). For the charac-
terization of surface properties, the measurements
of water, dimethylformamide (DMF) and ethyl-
ene glycol (MEG) contact angle on KVS Instru-
ment CAM 100 were recorded.
Cell culture. CaCo-2 cells from American
Type Culture Collection (ATTC) were grown in
advanced RPMI 1640 culture medium (Gibco,
12633-012) supplemented with 5% fetal bovine
serum (FBS) (Gibco, 10106-169) and
L-glutamine (2 mmol/l) without antibiotics, at
37°C in a CO2 incubator (5% CO2, 95% air, 95%
relative humidity). FBS was heat inactivated prior
to use (at 56°C for 30 min with gentle shaking).
FlexiPERM micro 12 wells (Vivascience IV-
50011436, growth area 0.3 cm2) were stacked on
microscope slides and VT079, VT104, VT111
and VT112 sample slides. The seeding number of
cells was 1.5x104 cell/ cm2.
Protein coatings. Laminin-1 from basement
membrane of Engelbreth-Holm-Swarm mouse
sarcoma (Sigma, L2020) was used. It was slowly
thawed in the refrigerator and diluted in Hank's
balanced salt solution (HBSS, Gibco, 14060-
040). The surface was coated with a minimal vol-
ume (18 µl) of working solution (0.1 mg/ml) and
left for 45 min. Excess fluid was sucked off and
the surface was left to air dry before introducing
the cell suspension. Fibronectin (Sigma F1141)
solution in PBS (0.2 mg/ml) was used for coat-
ings. A minimal amount (11 µl) of fibronectin
solution was added to each well and left to dry for
45 min. Excess fluid was sucked off. The coated
surface was rinsed with culture medium before
the cell suspension was added. Collagen-1 (Sig-
ma C7661) was dissolved in acetic acid
(0.1 mg/ml). A minimal amount (18 µl) of colla-
V. Tomkute, A. Beganskiene, A. Kareiva et al.
_____________________________________________________________________________________________
350 ХФТП 2010. Т. 1. № 3
gen solution was placed in each well and the col-
lagen was allowed to bind for two hours at 37ºC.
Excess fluid was sucked off and the surface was
left to air dry. The coated surface was rinsed with
HBSS before adding the cell suspension.
Assessment of cell proliferation. Prolifera-
tion of cells was measured with the colorimetric
cell proliferation BrdU (5-bromo-2-deoxyuridine)
test (Kit No. 1 647 229, Roche) two days post-
seeding on coated and non-coated VT samples
and glass. The supernatant was replaced by
100 µl of fresh growth medium and 10 µl BrdU
labelling solution was added. The cells were in-
cubated for 90 min at 37ºC. After removal of the
supernatant, 150 µl of FixDent solution was add-
ed and the cells were left at room temperature for
30 min. The supernatant was sucked off and 75 µl
of anti-BrdU-POD was added. The cells were
then stored for 90 min at room temperature. Af-
terwards, the cells were washed three times with
150 µl washing buffer. 75 µl of substrate solution
was added and left for some minutes protected
from light for the colour to develop. 75 µl of the
solution was transferred to 96-well microtiter
plates and 20 µl of 1M sulphuric acid was added.
The absorbance was measured at 450 nm. Data
were derived from three independent experiments
and presented as means with standard deviations.
The differences were analyzed using Student's t
test on two populations and One-way ANOVA;
p<0.01 was considered significant.
Fig. 1. Hybrid sol-gels coatings with different surface
properties synthesis scheme
RESULTS AND DISCUSSION
During the immobilization of various bio-
logical molecules on the sol-gel matrixes particu-
lar attention is paid to the bioactive surface prop-
erties (density of surface charge, hydrophilicity or
hydrophobicity, the chemical structure and func-
tional groups, surface topography and roughness, the
interfacial free energy, etc. [6]). Using sol-gel syn-
thesis method it is possible to prepare materials with
small pores (d<0.4 nm) and high specific surface area
(S
BET
>250 m
2
/g), different chemical structure, etc.
[3–6]. Total sol-gels coatings with different surface
properties synthesis scheme is shown in Fig. 1.
Characterization of the Silica Hybrids. Con-
tact angle on the coating films. Surface properties
that influence protein adsorption and subsequent
cell adhesion include surface free energy, rough-
ness and chemistry [17]. The prepared sol-gel
derived colloidal silica coatings and modified
coatings were characterized by wettability meas-
urements using the contact angle measurements
because hydrophilicity/hydrophobicity and sur-
face free energy (surface tension) is an important
thermodynamic characteristic of the surface of
liquids and solids. Solids with low surface free
energy hydrophobic and hydrophilic with solids
with high-energy [18]. One of the most frequently
used methods of contact angle assessments is the
sessile drop technique and the surface free energies
were estimated from the contact angles. Fig. 2
shows water drop on the different coating films.
Sol compositions and data of water, dimethylform-
amide (DMF) and ethylene glycol (MEG) contact
angles of coatings are shown in Table 1.
a
b
c
Fig. 2. Imagines of water drop on the differente coat-
ing films: a – SiO2 3% (θ=17°), b – PS 3%
(θ=64°), c – HMDS modified SiO2 (θ=144°)
Synthesis and Investigation of Modified Silica Coatings for Biotechnology
_____________________________________________________________________________________________
ХФТП 2010. Т. 1. № 3 351
Table 1. Sol composition and data of H2O, DMF and
MEG contact angles, surface free energies of
coatings
Contact angle (θ),°
Sample
No.
Sol
composition H2O DMF MEG
Surface
free energy
γs, mN/m
VT104 SiO2 (3%) 17±1 11±1 30±1 71
VT111
polysiloxane
(3%)
64±1 32±1 34±1 39
VT115
SiO2 (3%)/
HMDS (0.625%)
42±1 20±1 46±1 55
VT112
SiO2 (3%)/
HMDS (1.25%)
47±1 17±1 48±1 54
VT079
SiO2 (3%)/
HMDS (10%)
144±1 7±1 87±1 30
The results showed that the colloidal silica sur-
face (water contact angle 17°) is more hydrophilic
and its surface free energy is lower compare to PS
(water contact angle 64°). In the acid-catalyzed
TEOS hydrolysis system the protonation of the al-
koxide group causes electron density to be with-
drawn from Si allowing the nucleophilic attack from
water. In contrast, the base-catalyzed hydrolysis of
silicon alkoxides proceeds through the attack of a
nucleophilic deprotonated silanol on a neutral silicic
acid. In general, silicon oxide networks obtained via
acid-catalyzed conditions consist of linear or ran-
domly branched polymers; by contrast, base-
catalyzed systems result in highly branched clusters,
so contact angle measurements and IR spectra con-
firms that on the SiO2 coating film surface are more
–OH functional groups than on the PS.
With the aim of finding the optimal hydropho-
bicity for cells adhesion and growth, the methyl-
modified silica coating were prepared. The colloidal
silica particles are covered by hydroxyl groups, after
HMDS addition some of the hydroxyl groups are
replaced by methyl groups. It can be said that the
contact angle varies significantly with the mole ratio
of SiO2/HMDS by considering the accuracy. The
contact angle of water and surface free energy in-
creased with increasing amount of HMDS. In case
HMDS, each monomer of HMDS consists of two
trialkylsilane groups, which gets attached to the sur-
face. The HMDS modified silica coating (VT079)
show the highest contact angle (144°) and the low-
est SiO2 (VT104, 17°). The hydrophilic methyl
modified silica surfaces were prepared using
0.625% and 1.25% of HMDS during modification.
FTIR Characterization. FTIR characteriza-
tion of the SiO2, polysiloxane (PS), and with
HMDS modified SiO2 hybrids is shown in Fig. 3.
3500 3000 2500 2000 1500 1000 500
0,0
0,2
0,4
0,6
0,8
1,0
B
A
C
790
820
950
10601090
164023602950
A
bs
or
ba
nc
e
(a
.u
.)
Wavenumber (cm-1)
SiO
2
/HMDS
PS
SiO
2
3440
Fig. 3. FTIR spectra of the a – SiO2, b – polysiloxane
and c – SiO2 modified with HMDS
The SiO2, PS and HMDS gels exhibits hy-
droxyl absorption bands at 3450–3400 cm-1,
which arise from relatively free, non–hydrogen-
bonded, and hydrogen-bonded silanols (Si–OH),
respectively. The IR absorption bands at 1090
and 1060 cm-1 in the difference spectrums are
assigned to the Si–O vibrations and 790 cm-1
symmetric Si–O vibrations, 950 cm-1 attributed to
symmetric Si–OH vibrations. After the surface
modification with HMDS, the intensity of absorp-
tion bands at 2950 cm-1 corresponding to C–H,
–CH2–, –CH3 groups intensities are higher. The
overlapped absorption bands from 800 to 1260 cm-1
can be attributed to SiO2, Si–OH and organic
groups (C–H, –CH2–, –CH3).
AFM analysis of the coating films. Coatings
and the sol particles morphological studies were
carried out by atomic force microscope. As can
be seen from the AFM images (Fig. 4) that PS
coating is rather smooth, the surface roughness is
RMS=0.79 nm than other coatings. The surface of
SiO2 coating roughness is RMS=3.05 nm and the
particles show tendency to form bigger agglom-
erates when SiO2 is modified with HMDS (RMS is
increasing with increasing amount of HMDS). It
is evident from the images that the nanosilica sur-
face is modified by methyl groups.
Cell behaviour on biomaterial surface. The
CaCo-2 cells from American Type Culture Col-
lection (ATTC) were grown on sol-gel derived
coatings with different wettability properties. The
coatings obtained from colloidal silica (VT104),
polysiloxane sol (VT111) methyl-modified sols
(VT079 and VT112) were selected for prolifera-
tion test. The sol composition and data of water
contact angles, surface free energy and surface
roughness of coatings are shown in Table 2.
V. Tomkute, A. Beganskiene, A. Kareiva et al.
_____________________________________________________________________________________________
352 ХФТП 2010. Т. 1. № 3
Fig. 4. AFM imagines of modified surfaces: a – 3% SiO2
(RMS=3.05 nm, VT104); b – 3% polysiloxane
(RMS=0.79 nm, VT111); c – SiO2 (3%)/HMDS
(1.25%) (RMS=2.55 nm, VT112); d – SiO2
(3%)/HMDS (10%) (RMS=3.27 nm, VT079)
Table 2. The sol composition and data of water contact
angles, surface free energy and surface rough-
ness of hybrid silica coatings tested for the pro-
liferation and adhesion test of CaCo-2 cells
Sol
No.
Sample
No.
Functional
groups on
the surface
Surface
roughness
(RMS), nm
Contact
angle (θ),°
Surface free
energy
γs, mN/m
A
VT
104
–OH 3.05 17±1 71
B
VT
111
–OH 0.79 64±1 39
C2
VT
112
–OH,
–CH3
2.55 47±1 54
C3
VT
079
–OH,
–CH3
3.27 144±1 30
Cell behaviour on biomaterial surface is
modulated by the concentration, composition and
conformation of absorbed extracellular matrix
(ECM) proteins [19]. The sol-gel coatings were
coated with a minimal volume of different pro-
teins as laminin, fibronectin or collagen-1 solu-
tions. AFM imagines of protein coated silica sur-
face are shown in Fig. 5 and Fig. 6.
Fig. 5. AFM imagines of fibronectin coating on: a –
glass; b – 3% polysiloxane (VT111); c –
SiO2:HMDS (VT112) sol-gel modified samples
Fig. 6. AFM imagines of Collagen-1 coating on: a –
glass; b – 3% polysiloxane (VT111); c –
SiO2:HMDS (VT112) sol-gel modified samples
Results of proliferation on coatings surfaces
are presented in Fig. 7 and Fig. 8.
Surfaces
0,0
0,1
0,2
0,3
A
b
so
rp
ti
o
n
a
t
45
0
n
m
Glass VT079 VT104 VT111 VT112
Fig. 7. Proliferation of CaCo-2 cells on VT079,
VT104, VT111, VT112 samples and glass as
control surface
When only simple samples (Fig. 7) are com-
pared the means within all samples are signifi-
cantly different (p<0.01, One-way ANOVA).
Proliferation on VT079 was significantly slower
and on VT112 significantly faster (p<0.01) then
on glass control (Fig. 7). This two substrates
were both methyl-modified, however contend of
HMDS was 10% in VT079 and 1.25% in
VT112.
Control Laminin Fibronectin Collagen-1
0,0
0,2
0,4
0,6
0,8
1,0
A
bs
or
pt
io
n
at
4
50
n
m
VT079
VT104
VT111
VT112
Fig. 8. Proliferation of CaCo-2 cells on laminin-1,
fibronectin and collagen-1 coated VT079,
VT104, VT111 and VT112 samples and cor-
responding controls
Synthesis and Investigation of Modified Silica Coatings for Biotechnology
_____________________________________________________________________________________________
ХФТП 2010. Т. 1. № 3 353
The means within the surfaces for all protein
coated VT samples and controls are significantly
different (p<0.01, One-way ANOVA) and almost
all protein coated samples stimulated proliferation
compared to the corresponding controls (Fig. 8).
Laminin-1 coated VT104 sample was the only
protein coated surface where proliferation was
significantly slower (p<0.01) then on control.
CONCLUSIONS
The modified sol-gel derived silica coatings
were prepared and characterized. The topography
of protein coatings differs distinctly between
glass and various sol-gel modified samples. The
coatings of colloidal silica (VT104, water contact
angle 17°), polysiloxane sol (VT111, 64°) me-
thyl-modified sols (VT079, 144° and VT112,
47°) with various wettability properties were
tested for CaCo-2 cells proliferation. Methyl-
modified coating VT112 proved to be the best
substrate for cell proliferation. Cell proliferation
two days post seeding was significantly faster on
almost all proteins coated samples compared to
corresponding controls. The difference in drop
contact angle between VT079 (144°) and VT104
(17°) is the biggest. From results we can assume,
that surface characteristic contact angle and func-
tional groups are significant for protein adsorp-
tion and consecutive for cell growth and prolif-
eration. The advent of hybrid sol-gel materials
opens new possibilities for tailoring efficient sub-
strates for cell adhesion and growth. A major ad-
vantage of these materials is that the quantity of
preparation methods and chemicals with which
one can design almost any desired surface prop-
erty is practically unlimited.
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Received 20.05.2010, accepted 17.08.2010
Синтез та дослідження модифікованих кремнеземних покриттів для біотехнології
В. Томкуте, А. Беганскієне, А. Карейва, С. Земліч Джохадар, У. Батіста
Кафедра загальної та неорганічної хімії, Вільнюський університет
вул. Наугардуко 24, Вільнюс Lt-3225, Литва
Факультет медицини, Інститут біофізики
вул. Ліпічева 2, Любляна SI-1000, Словенія
Для тестування адгезії білків золь-гель методом приготовано мезопоруваті органо-неорганічні гібридні
композити на скляних підкладинках. Різні типи гідрофобних та гідрофільних кремнеземних золів та гелів
було приготовано з використанням тетраетоксисилану (ТЕОС) як вихідного матеріалу і модифіковано гек-
саметилдисилазаном (ГМДС). Тонкі золь-гель плівки було успішно приготовано за допомогою процедури гла-
зурування зануренням на скляних поверхнях. Одержано характеристики поверхонь покриттів. Одержані
шляхом золь-гель синтезу покриття – похідні колоїдного кремнезему та модифіковані покриття було оха-
рактеризовано вимірюванням змочуваності. Для характеризації поверхонь було також використано аналіз
за допомогою інфрачервоної спектроскопії та атомної силової мікроскопії (АСМ). Покриття з колоїдного
кремнезему (VT104, кут змочування водою 17°), полісилоксановий золь (VT111, 64°), метил-модифіковані золі
(VT079, 144° та VT112, 47°) з різною змочуваністю було протестовано для розмноження клітин СаСо-2.
Метил-модифіковане покриття VT112 виявилось найкращим субстратом для розмноження клітин.
Синтез и исследование модифицированных кремнеземных покрытий для биотехнологии
В. Томкуте, А. Беганскиене, А. Карейва, С. Землич Джохадар, У. Батиста
Кафедра общей и неорганической химии, Вильнюсский университет
ул. Наугардуко 24, Вильнюс Lt-3225, Литва
Факультет медицины, Институт биофизики
ул. Липичева 2, Любляна SI-1000, Словения
Для тестирования адгезии белков золь-гель методом приготовлены мезопористые органо-
неорганические гибридные композиты на стеклянных подложках. Разные типы гидрофобных и гидрофиль-
ных кремнеземных золей и гелей были приготовлены с использованием тетраэтоксисилана (ТЭОС) как ис-
ходного материала и модифицированы гексаметилдисилазаном (ГМДС). Тонкие золь-гель пленки были ус-
пешно приготовлены с помощью процедуры глазурирования погружением на стеклянных поверхностях. По-
лучены характеристики поверхностей покрытий. Полученные путем золь-гель синтеза покрытия – произ-
водные коллоидного кремнезема и модифицированные покрытия были охарактеризованы измерением смачи-
ваемости. Для характеризации поверхностей был также использован анализ с помощью инфракрасной
спектроскопии и атомной силовой микроскопии (АСМ). Покрытия из коллоидного кремнезема (VT104, угол
смачивания водой 17°), полисилоксановый золь (VT111, 64°), метил-модифицированные золи (VT079, 144° и
VT112, 47°) с разной смачиваемостью были протестированы для размножения клеток СаСо-2. Метил-
модифицированное покрытие VT112 оказалось наилучшим субстратом для размножения клеток.
|
| id | nasplib_isofts_kiev_ua-123456789-29006 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 2079-1704 |
| language | English |
| last_indexed | 2025-12-07T15:19:30Z |
| publishDate | 2010 |
| publisher | Інститут хімії поверхні ім. О.О. Чуйка НАН України |
| record_format | dspace |
| spelling | Tomkute, V. Beganskiene, A. Kareiva, A. Zemljic Jokhadar, S. Batista, U. 2011-11-27T18:09:15Z 2011-11-27T18:09:15Z 2010 Synthesis and Investigation of Modified Silica Coatings for Biotechnology / V. Tomkute, A. Beganskiene, A. Kareiva, S. Zemljic Jokhadar, U Batista // Хімія, фізика та технологія поверхні. — 2010. — Т. 1, № 3. — С. 348-354. — Бібліогр.: 19 назв. — англ. 2079-1704 https://nasplib.isofts.kiev.ua/handle/123456789/29006 544.723 Mesoporous organic-inorganic hybrid composites on glass substrates were prepared by the sol-gel method for testing the proteins adhesion. Different types of hydrophobic/hydrophilic silica sol-gels were prepared using tetraethoxysilane (TEOS) as starting material and modified with hexamethyldisilazane (HMDS). Sol-gel thin films were successfully prepared with the dip-coating technique on glass surfaces. The coatings surface characteristics were evaluated. The prepared sol-gel derived colloidal silica coatings and modified coatings were characterized by wettability measurements. Also, infrared spectroscopy, atomic force microscope (AFM) assay were used to characterise the surfaces. The coatings of colloidal silica (VT104, water contact angle 17°), polysiloxane sol (VT111, 64°) methyl-modified sols (VT079, 144° and VT112, 47°) with various wettability properties were tested for CaCo-2 cells proliferation. Methylmodified coating VT112 proved to be the best substrate for cell proliferation. Для тестування адгезії білків золь-гель методом приготовано мезопоруваті органо-неорганічні гібридні композити на скляних підкладинках. Різні типи гідрофобних та гідрофільних кремнеземних золів та гелів було приготовано з використанням тетраетоксисилану (ТЕОС) як вихідного матеріалу і модифіковано гексаметилдисилазаном (ГМДС). Тонкі золь-гель плівки було успішно приготовано за допомогою процедури глазурування зануренням на скляних поверхнях. Одержано характеристики поверхонь покриттів. Одержані шляхом золь-гель синтезу покриття – похідні колоїдного кремнезему та модифіковані покриття було охарактеризовано вимірюванням змочуваності. Для характеризації поверхонь було також використано аналіз за допомогою інфрачервоної спектроскопії та атомної силової мікроскопії (АСМ). Покриття з колоїдного кремнезему (VT104, кут змочування водою 17°), полісилоксановий золь (VT111, 64°), метил-модифіковані золі (VT079, 144° та VT112, 47°) з різною змочуваністю було протестовано для розмноження клітин СаСо-2. Метил-модифіковане покриття VT112 виявилось найкращим субстратом для розмноження клітин. Для тестирования адгезии белков золь-гель методом приготовлены мезопористые органо-неорганические гибридные композиты на стеклянных подложках. Разные типы гидрофобных и гидрофильных кремнеземных золей и гелей были приготовлены с использованием тетраэтоксисилана (ТЭОС) как исходного материала и модифицированы гексаметилдисилазаном (ГМДС). Тонкие золь-гель пленки были успешно приготовлены с помощью процедуры глазурирования погружением на стеклянных поверхностях. Получены характеристики поверхностей покрытий. Полученные путем золь-гель синтеза покрытия – производные коллоидного кремнезема и модифицированные покрытия были охарактеризованы измерением смачиваемости. Для характеризации поверхностей был также использован анализ с помощью инфракрасной спектроскопии и атомной силовой микроскопии (АСМ). Покрытия из коллоидного кремнезема (VT104, угол смачивания водой 17°), полисилоксановый золь (VT111, 64°), метил-модифицированные золи (VT079, 144° и VT112, 47°) с разной смачиваемостью были протестированы для размножения клеток СаСо-2. Метилмодифицированное покрытие VT112 оказалось наилучшим субстратом для размножения клеток. en Інститут хімії поверхні ім. О.О. Чуйка НАН України Хімія, фізика та технологія поверхні Біомедичні аспекти поверхневих явищ Synthesis and Investigation of Modified Silica Coatings for Biotechnology Синтез та дослідження модифікованих кремнеземних покриттів для біотехнології Синтез и исследование модифицированных кремнеземных покрытий для биотехнологии Article published earlier |
| spellingShingle | Synthesis and Investigation of Modified Silica Coatings for Biotechnology Tomkute, V. Beganskiene, A. Kareiva, A. Zemljic Jokhadar, S. Batista, U. Біомедичні аспекти поверхневих явищ |
| title | Synthesis and Investigation of Modified Silica Coatings for Biotechnology |
| title_alt | Синтез та дослідження модифікованих кремнеземних покриттів для біотехнології Синтез и исследование модифицированных кремнеземных покрытий для биотехнологии |
| title_full | Synthesis and Investigation of Modified Silica Coatings for Biotechnology |
| title_fullStr | Synthesis and Investigation of Modified Silica Coatings for Biotechnology |
| title_full_unstemmed | Synthesis and Investigation of Modified Silica Coatings for Biotechnology |
| title_short | Synthesis and Investigation of Modified Silica Coatings for Biotechnology |
| title_sort | synthesis and investigation of modified silica coatings for biotechnology |
| topic | Біомедичні аспекти поверхневих явищ |
| topic_facet | Біомедичні аспекти поверхневих явищ |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/29006 |
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