Влияние предварительного замораживания и низкотемпературного хранения на взаимодействие ацетилхолинэстеразы с озоном

• Date: 2007
• Main Authors: Грищенко, В.И., Дюбко, Т.С., Зинченко, В.Д.
• Format: Article
• Language: Russian
• Published: Видавничий дім "Академперіодика" НАН України 2007
• Subjects: Біологія
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/3828
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Влияние предварительного замораживания и низкотемпературного хранения на взаимодействие ацетилхолинэстеразы с озоном / В.И. Грищенко, Т.С. Дюбко, В.Д. Зинченко // Доп. НАН України. — 2007. — № 12. — С. 150-155. — Бібліогр.: 15 назв. — рос.

Ozone (O₃) effect on the conformation state and the enzyme activity of acetylcholinesterase (CE) in solutions subjected to freezing by two regimes: “slow” down to −18 °C (I regime) and “rapid” down to −196 °C (II regime) with the following storage for 1 week under corresponding temperatures has been studied by using the methods of optical pectroscopy. Freezing rate and storage temperature were established to affect the CE conformation state determining the protein sensitivity to the following ozone treatment. The enzyme samples treated according to regime I are characterized by the highest sensitivity to increasing ozone doses. A feasible relation of sensitivity to the ozone effect of CE samples, treated according to regimes I and II, with differences in the structure of molecular aggregates formed at the “rapid” and “slow” freezing of the enzyme solution is discussed.