Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer

• Date: 2009
• Main Authors: Gryshkova, V.S., Lituyev, D.S., Filonenko, V.V., Kiyamova, R.G.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2009
• Subjects: Структура і функції біополімерів
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/5651
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer / V.S. Gryshkova, D.S. Lituyev, V.V. Filonenko, R.G. Kiyamova // Біополімери і клітина. — 2009. — Т. 25, № 2. — С. 95-100. — Бібліогр.: 23 назв. — англ.

The aim of present study was to develop a model for a functional analysis of a recently identified marker of the ovarian cancer - sodium-dependent phosphate transporter NaPi2b. For this purpose, we have created HEK293 stable cell lines expressing wild type or mutant forms of NaPi2b (T330V substitution in a large extracellular loop and a 6 amino acid residues deletion in the C-terminal cytoplasmic tail), revealed in the ovarian cancer cell lines. The expression of wild type and mutant forms NaPi2b in the stable cell lines created was confirmed by Western-blot analysis with monoclonal antibodies against NaPi2b. The cellular models described here will be useful for studying the function of sodium-dependent phosphate transporter NaPi2b in health and disease.