Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer

Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer

The aim of present study was to develop a model for a functional analysis of a recently identified marker of the ovarian cancer - sodium-dependent phosphate transporter NaPi2b. For this purpose, we have created HEK293 stable cell lines expressing wild type or mutant forms of NaPi2b (T330V substituti...

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Date:2009
Main Authors: Gryshkova, V.S., Lituyev, D.S., Filonenko, V.V., Kiyamova, R.G.
Format: Article
Language:English
Published: Інститут молекулярної біології і генетики НАН України 2009
Subjects:
Структура і функції біополімерів
Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/5651
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer / V.S. Gryshkova, D.S. Lituyev, V.V. Filonenko, R.G. Kiyamova // Біополімери і клітина. — 2009. — Т. 25, № 2. — С. 95-100. — Бібліогр.: 23 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-5651
record_format dspace
spelling Gryshkova, V.S.
Lituyev, D.S.
Filonenko, V.V.
Kiyamova, R.G.
2010-02-01T14:19:49Z
2010-02-01T14:19:49Z
2009
Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer / V.S. Gryshkova, D.S. Lituyev, V.V. Filonenko, R.G. Kiyamova // Біополімери і клітина. — 2009. — Т. 25, № 2. — С. 95-100. — Бібліогр.: 23 назв. — англ.
0233-7657
https://nasplib.isofts.kiev.ua/handle/123456789/5651
577.2, 577.27
The aim of present study was to develop a model for a functional analysis of a recently identified marker of the ovarian cancer - sodium-dependent phosphate transporter NaPi2b. For this purpose, we have created HEK293 stable cell lines expressing wild type or mutant forms of NaPi2b (T330V substitution in a large extracellular loop and a 6 amino acid residues deletion in the C-terminal cytoplasmic tail), revealed in the ovarian cancer cell lines. The expression of wild type and mutant forms NaPi2b in the stable cell lines created was confirmed by Western-blot analysis with monoclonal antibodies against NaPi2b. The cellular models described here will be useful for studying the function of sodium-dependent phosphate transporter NaPi2b in health and disease.
en
Інститут молекулярної біології і генетики НАН України
Структура і функції біополімерів
Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer
Створення клітинних моделей для аналізу Na-залежного фосфатного транспортера NaPi2b - потенційного маркера раку яєчників
Создание клеточных моделей для анализа Na-зависимого фосфатного транспортера NaPi2b – потенциального маркера рака яичников
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer
spellingShingle Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer
Gryshkova, V.S.
Lituyev, D.S.
Filonenko, V.V.
Kiyamova, R.G.
Структура і функції біополімерів
title_short Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer
title_full Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer
title_fullStr Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer
title_full_unstemmed Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer
title_sort creation of cellular models for the analysis of sodium-dependent phosphate transporter napi2b, a potential marker for ovarian cancer
author Gryshkova, V.S.
Lituyev, D.S.
Filonenko, V.V.
Kiyamova, R.G.
author_facet Gryshkova, V.S.
Lituyev, D.S.
Filonenko, V.V.
Kiyamova, R.G.
topic Структура і функції біополімерів
topic_facet Структура і функції біополімерів
publishDate 2009
language English
publisher Інститут молекулярної біології і генетики НАН України
format Article
title_alt Створення клітинних моделей для аналізу Na-залежного фосфатного транспортера NaPi2b - потенційного маркера раку яєчників
Создание клеточных моделей для анализа Na-зависимого фосфатного транспортера NaPi2b – потенциального маркера рака яичников
description The aim of present study was to develop a model for a functional analysis of a recently identified marker of the ovarian cancer - sodium-dependent phosphate transporter NaPi2b. For this purpose, we have created HEK293 stable cell lines expressing wild type or mutant forms of NaPi2b (T330V substitution in a large extracellular loop and a 6 amino acid residues deletion in the C-terminal cytoplasmic tail), revealed in the ovarian cancer cell lines. The expression of wild type and mutant forms NaPi2b in the stable cell lines created was confirmed by Western-blot analysis with monoclonal antibodies against NaPi2b. The cellular models described here will be useful for studying the function of sodium-dependent phosphate transporter NaPi2b in health and disease.
issn 0233-7657
url https://nasplib.isofts.kiev.ua/handle/123456789/5651
citation_txt Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer / V.S. Gryshkova, D.S. Lituyev, V.V. Filonenko, R.G. Kiyamova // Біополімери і клітина. — 2009. — Т. 25, № 2. — С. 95-100. — Бібліогр.: 23 назв. — англ.
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first_indexed 2025-12-07T17:42:11Z
last_indexed 2025-12-07T17:42:11Z
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