Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer
The aim of present study was to develop a model for a functional analysis of a recently identified marker of the ovarian cancer - sodium-dependent phosphate transporter NaPi2b. For this purpose, we have created HEK293 stable cell lines expressing wild type or mutant forms of NaPi2b (T330V substituti...
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| Datum: | 2009 |
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| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | Englisch |
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Інститут молекулярної біології і генетики НАН України
2009
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| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/5651 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer / V.S. Gryshkova, D.S. Lituyev, V.V. Filonenko, R.G. Kiyamova // Біополімери і клітина. — 2009. — Т. 25, № 2. — С. 95-100. — Бібліогр.: 23 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862713233740136448 |
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| author | Gryshkova, V.S. Lituyev, D.S. Filonenko, V.V. Kiyamova, R.G. |
| author_facet | Gryshkova, V.S. Lituyev, D.S. Filonenko, V.V. Kiyamova, R.G. |
| citation_txt | Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer / V.S. Gryshkova, D.S. Lituyev, V.V. Filonenko, R.G. Kiyamova // Біополімери і клітина. — 2009. — Т. 25, № 2. — С. 95-100. — Бібліогр.: 23 назв. — англ. |
| collection | DSpace DC |
| description | The aim of present study was to develop a model for a functional analysis of a recently identified marker of the ovarian cancer - sodium-dependent phosphate transporter NaPi2b. For this purpose, we have created HEK293 stable cell lines expressing wild type or mutant forms of NaPi2b (T330V substitution in a large extracellular loop and a 6 amino acid residues deletion in the C-terminal cytoplasmic tail), revealed in the ovarian cancer cell lines. The expression of wild type and mutant forms NaPi2b in the stable cell lines created was confirmed by Western-blot analysis with monoclonal antibodies against NaPi2b. The cellular models described here will be useful for studying the function of sodium-dependent phosphate transporter NaPi2b in health and disease.
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| first_indexed | 2025-12-07T17:42:11Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-5651 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 0233-7657 |
| language | English |
| last_indexed | 2025-12-07T17:42:11Z |
| publishDate | 2009 |
| publisher | Інститут молекулярної біології і генетики НАН України |
| record_format | dspace |
| spelling | Gryshkova, V.S. Lituyev, D.S. Filonenko, V.V. Kiyamova, R.G. 2010-02-01T14:19:49Z 2010-02-01T14:19:49Z 2009 Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer / V.S. Gryshkova, D.S. Lituyev, V.V. Filonenko, R.G. Kiyamova // Біополімери і клітина. — 2009. — Т. 25, № 2. — С. 95-100. — Бібліогр.: 23 назв. — англ. 0233-7657 https://nasplib.isofts.kiev.ua/handle/123456789/5651 577.2, 577.27 The aim of present study was to develop a model for a functional analysis of a recently identified marker of the ovarian cancer - sodium-dependent phosphate transporter NaPi2b. For this purpose, we have created HEK293 stable cell lines expressing wild type or mutant forms of NaPi2b (T330V substitution in a large extracellular loop and a 6 amino acid residues deletion in the C-terminal cytoplasmic tail), revealed in the ovarian cancer cell lines. The expression of wild type and mutant forms NaPi2b in the stable cell lines created was confirmed by Western-blot analysis with monoclonal antibodies against NaPi2b. The cellular models described here will be useful for studying the function of sodium-dependent phosphate transporter NaPi2b in health and disease. en Інститут молекулярної біології і генетики НАН України Структура і функції біополімерів Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer Створення клітинних моделей для аналізу Na-залежного фосфатного транспортера NaPi2b - потенційного маркера раку яєчників Создание клеточных моделей для анализа Na-зависимого фосфатного транспортера NaPi2b – потенциального маркера рака яичников Article published earlier |
| spellingShingle | Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer Gryshkova, V.S. Lituyev, D.S. Filonenko, V.V. Kiyamova, R.G. Структура і функції біополімерів |
| title | Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer |
| title_alt | Створення клітинних моделей для аналізу Na-залежного фосфатного транспортера NaPi2b - потенційного маркера раку яєчників Создание клеточных моделей для анализа Na-зависимого фосфатного транспортера NaPi2b – потенциального маркера рака яичников |
| title_full | Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer |
| title_fullStr | Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer |
| title_full_unstemmed | Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer |
| title_short | Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer |
| title_sort | creation of cellular models for the analysis of sodium-dependent phosphate transporter napi2b, a potential marker for ovarian cancer |
| topic | Структура і функції біополімерів |
| topic_facet | Структура і функції біополімерів |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/5651 |
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