Electron Microscopical Study of Spermatozoa of the Cestode Dilepis undula (Cyclophyllidea, Dilepididae)
The fine structure of spermatozoon of the dilepidid cestode Dilepis undula is described. The main structural elements of the sperm cell are: 1) plasma membrane and underlying single row of cortical microtubules; 2) a single axoneme of the unique platyhelminth 9+˝1˝ type; 3) periaxonemal sheath compo...
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Swiderski, Z. Salamatin, R.V. Tkach, V.V. 2014-06-15T18:42:48Z 2014-06-15T18:42:48Z 2000 Electron Microscopical Study of Spermatozoa of the Cestode Dilepis undula (Cyclophyllidea, Dilepididae) / Z. Swiderski, R.V. Salamatin, V.V. Tkach // Вестник зоологии. — 2000. — Т. 34, № 4-5. — С. 93-97. — Бібліогр.: 12 назв. — англ. 0084-5604 https://nasplib.isofts.kiev.ua/handle/123456789/64496 595.121 The fine structure of spermatozoon of the dilepidid cestode Dilepis undula is described. The main structural elements of the sperm cell are: 1) plasma membrane and underlying single row of cortical microtubules; 2) a single axoneme of the unique platyhelminth 9+˝1˝ type; 3) periaxonemal sheath composed of the crystalline-like material; 4) elongated electron-dense nucleus; 5) crested body. The similarities and differences between the ultrastructure of spermatozoa of D. undula and that of some other cestodes, are briefly discussed. Описано тонкое строение сперматозоидов Dilepis undula. Основными структурными элементами сперматозоида являются: 1) плазматическая мембрана с подлежащим одним рядом кортикальных микротрубочек; 2) единственная аксонема, устроенная по типу 9+1, уникальному для плоских червей; 3) цилиндрический периаксонемальный футляр, образованный из кристаллоподобного материала; 4) удлиненное электронно-плотное ядро и 5) гребневидное тело. Кратко обсуждены сходства и различия в структуре сперматозоидов у D. undula и некоторых других цестод The authors wish to thank Dr Yuri Kuzmin (Institute of Zoology NANU, Kyiv) for his technical assistance. The research was carried out in the framework of the scientific co-operation between the Institute of Zoology of the Ukrainian National Academy of Sciences and the Institute of Parasitology of the Polish Academy of Sciences. This study was financially supported by the grant ¹ 6 PO4Ñ 01314 from the Polish Committee for Scientific Research (KBN) and by a fellowship from the J. Mianowski Foundation (Warsaw, Poland) to R. Salamatin. en Інститут зоології ім. І.І. Шмальгаузена НАН України Вестник зоологии Краткие сообщения Electron Microscopical Study of Spermatozoa of the Cestode Dilepis undula (Cyclophyllidea, Dilepididae) Электронно-микроскопическое исследование сперматозоидов цестоды Dilepis undula (Cyclophyllidea, Dilepididae) Article published earlier |
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Electron Microscopical Study of Spermatozoa of the Cestode Dilepis undula (Cyclophyllidea, Dilepididae) |
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Electron Microscopical Study of Spermatozoa of the Cestode Dilepis undula (Cyclophyllidea, Dilepididae) Swiderski, Z. Salamatin, R.V. Tkach, V.V. Краткие сообщения |
| title_short |
Electron Microscopical Study of Spermatozoa of the Cestode Dilepis undula (Cyclophyllidea, Dilepididae) |
| title_full |
Electron Microscopical Study of Spermatozoa of the Cestode Dilepis undula (Cyclophyllidea, Dilepididae) |
| title_fullStr |
Electron Microscopical Study of Spermatozoa of the Cestode Dilepis undula (Cyclophyllidea, Dilepididae) |
| title_full_unstemmed |
Electron Microscopical Study of Spermatozoa of the Cestode Dilepis undula (Cyclophyllidea, Dilepididae) |
| title_sort |
electron microscopical study of spermatozoa of the cestode dilepis undula (cyclophyllidea, dilepididae) |
| author |
Swiderski, Z. Salamatin, R.V. Tkach, V.V. |
| author_facet |
Swiderski, Z. Salamatin, R.V. Tkach, V.V. |
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Краткие сообщения |
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Краткие сообщения |
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2000 |
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English |
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Вестник зоологии |
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Інститут зоології ім. І.І. Шмальгаузена НАН України |
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Article |
| title_alt |
Электронно-микроскопическое исследование сперматозоидов цестоды Dilepis undula (Cyclophyllidea, Dilepididae) |
| description |
The fine structure of spermatozoon of the dilepidid cestode Dilepis undula is described. The main structural elements of the sperm cell are: 1) plasma membrane and underlying single row of cortical microtubules; 2) a single axoneme of the unique platyhelminth 9+˝1˝ type; 3) periaxonemal sheath composed of the crystalline-like material; 4) elongated electron-dense nucleus; 5) crested body. The similarities and differences between the ultrastructure of spermatozoa of D. undula and that of some other cestodes, are briefly discussed.
Описано тонкое строение сперматозоидов Dilepis undula. Основными структурными элементами сперматозоида являются: 1) плазматическая мембрана с подлежащим одним рядом кортикальных микротрубочек; 2) единственная аксонема, устроенная по типу 9+1, уникальному для плоских червей; 3) цилиндрический периаксонемальный футляр, образованный из кристаллоподобного материала; 4) удлиненное электронно-плотное ядро и 5) гребневидное тело. Кратко обсуждены сходства и различия в структуре сперматозоидов у D. undula и некоторых других цестод
|
| issn |
0084-5604 |
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https://nasplib.isofts.kiev.ua/handle/123456789/64496 |
| citation_txt |
Electron Microscopical Study of Spermatozoa of the Cestode Dilepis undula (Cyclophyllidea, Dilepididae) / Z. Swiderski, R.V. Salamatin, V.V. Tkach // Вестник зоологии. — 2000. — Т. 34, № 4-5. — С. 93-97. — Бібліогр.: 12 назв. — англ. |
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2025-11-24T05:44:13Z |
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Vestnik zoologii, 34(4—5): 93—97, 2000
© 2000 Z. Swiderski, R. V. Salamatin, V. V. Tkach
UDC 595.121
ELECTRON MICROSCOPICAL STUDY
OF SPERMATOZOA OF THE CESTODE DILEPIS UNDULA
(CYCLOPHYLLIDEA, DILEPIDIDAE)
Z. Swiderski1, R. V. Salamatin2, V. V. Tkach2
1W. Stefański Institute of Parasitology, Polish Academy of Sciences, 51/55 Twarda st., 00-818 Warsaw, Poland
2I. I. Schmalhausen Institute of Zoology, vul. B. Khmelnits’kogo, 15, Kyiv-30, MSP, 01601 Ukraine
Accepted 25 January 2000
Electron Microscopical Study of Spermatozoa of the Cestode Dilepis undula (Cyclophyllidea, Dilepididae). Swid-
erski Z., Salamatin R. V., Tkach V. V. – The fine structure of spermatozoon of the dilepidid cestode Dilepis
undula is described. The main structural elements of the sperm cell are: 1) plasma membrane and underlying
single row of cortical microtubules; 2) a single axoneme of the unique platyhelminth 9+˝1˝ type;
3) periaxonemal sheath composed of the crystalline-like material; 4) elongated electron-dense nucleus;
5) crested body. The similarities and differences between the ultrastructure of spermatozoa of D. undula and
that of some other cestodes, are briefly discussed.
Key wo rd s : spermatozoa, ultrastructure, Cestoda, Dilepididae, Dilepis undula.
Ýëåêòðîííî-ìèêðîñêîïè÷åñêîå èññëåäîâàíèå ñïåðìàòîçîèäîâ öåñòîäû Dilepis undula (Cyclophyllidea,
Dilepididae). Ñâèäåðñêèé Ç., Ñàëàìàòèí Ð. Â., Òêà÷ Â. Â. – Îïèñàíî òîíêîå ñòðîåíèå ñïåðìàòîçîèäîâ
Dilepis undula. Îñíîâíûìè ñòðóêòóðíûìè ýëåìåíòàìè ñïåðìàòîçîèäà ÿâëÿþòñÿ: 1) ïëàçìàòè÷åñêàÿ
ìåìáðàíà ñ ïîäëåæàùèì îäíèì ðÿäîì êîðòèêàëüíûõ ìèêðîòðóáî÷åê; 2) åäèíñòâåííàÿ àêñîíåìà, óñò-
ðîåííàÿ ïî òèïó 9+˝1˝, óíèêàëüíîìó äëÿ ïëîñêèõ ÷åðâåé; 3) öèëèíäðè÷åñêèé ïåðèàêñîíåìàëüíûé
“ôóòëÿð”, îáðàçîâàííûé èç êðèñòàëëîïîäîáíîãî ìàòåðèàëà; 4) óäëèíåííîå ýëåêòðîííî-ïëîòíîå ÿäðî è
5) ãðåáíåâèäíîå òåëî. Êðàòêî îáñóæäåíû ñõîäñòâà è ðàçëè÷èÿ â ñòðóêòóðå ñïåðìàòîçîèäîâ ó D. undula
è íåêîòîðûõ äðóãèõ öåñòîä.
Êëþ÷åâûå ñëîâà : ñïåðìàòîçîèäû, óëüòðàñòðóêòóðà, Cestoda, Dilepididae, Dilepis undula.
Inroduction
Ultrastructural characteristics of spermatozoa provide important criteria for the systematics and analysis of the
phylogenetic relationships among cestodes (Swiderski, 1968, 1970; Euzet et al., 1981; Justine, 1991, 1995, 1998; Bâ,
Marchand, 1995).
Up to now, data on the spermatozoa ultrastructure of about 70 cestode species belonging to all 12 recognised
orders of Eucestoda, are available in the literature (see Justine, 1991, 1998; Bâ, Marchand, 1995). For more detailed
analysis, however, spermatozoa of more cestode taxa should be studied. According to our knowledge, ultrastructure
of spermatozoa of only one dilepidid species, Molluscotaenia crassiscolex, has been examined so far (Swiderski,
Tkach, 1996). Miquel and Marchand (1997) in their paper on the spermatozoa of Dipylidium caninum noted that it
was the first study on the representative of Dilepididae. However, in their later paper (Miquel, Marchand, 1998) the
authors corrected this systematic allocation of D. caninum which, according to the widely accepted classification
(Jones, 1994), belongs to the family Dipylidiidae. The aim of the present study is to describe the fine structure of
spermatozoon of the cestode Dilepis undula which is a characteristic representative of the family Dilepididae and a
type species of the genus Dilepis.
Material and methods
Adult specimens of Dilepis undula (Schrank, 1788), containing mature and pregravid proglottids, were removed
from the intestine of a fieldfare (Turdus pilaris), from the vicinity of Kyiv, Ukraine. The proglottids were quickly
rinsed in saline, cut into small pieces and fixed in 4% glutaraldehyde in sodium cacodylate buffer (pH 7.4). After
washing in the same buffer and postfixation for 2 h in 1% OsO4, they were dehydrated in an ethanol series, infil-
trated with propylene oxide and embedded in Spurr's resin. Ultrathin sections, mounted on uncoated copper grids,
double stained with lead citrate and uranyl acetate, were examined under a JEM-1200EX electron microscope oper-
ated at an accelerateing voltage of 80 kV.
Z. Swiderski, R. V. Salamatin, V. V. Tkach 94
Results
Mature spermatozoa of D. undula are strongly elongated, filiform cells, tapered at both
extremities and lacking mitochondria. They measure about 300—350 μm in length and their
maximum diameter, in the nucleus containing region, is 0,5—0,6 μm.
Fig. 1. Diagram and corresponding reconstruction of the spermatozoon of D. undula from the representative cross-
sections of consecutive regions along longitudinal axis of sperm body.
Abbreviations to all figures: Ax – axoneme; c – core; Cb – crested body; cm – central matrix; CU – central core
unit of the axoneme; cx – cortex; DA – dynein arms; DPT – double peripheral tubules; M – plasma membrane;
Mt – microtubules; N – nucleus; P – periaxonemal sheath; s – spokes; sf A – subfiber A or tubule A; sf B – subfi-
ber B or tubule B.
Ðèñ. 1. Äèàãðàììà è ñîîòâåòñòâóþùàÿ ðåêîíñòðóêöèÿ ñïåðìàòîçîèäà D. undula, ñîñòàâëåííàÿ èç ïîïåðå÷íûõ
ñðåçîâ ó÷àñòêîâ, ïîñëåäîâàòåëüíî ðàñïîëîæåííûõ âäîëü ïðîäîëüíîé îñè ñïåðìàòîçîèäà.
Ñîêðàùåíèÿ êî âñåì èëëþñòðàöèÿì: Ax – àêñîíåìà; c – öåíòðàëüíûé ýëåêòðîííî-ïëîòíûé öèëèíäð ñåðäöå-
âèííîé ÷àñòè àêñîíåìû; Cb – ãðåáíåâèäíîå òåëî; cm – öåíòðàëüíûé ìàòðèêñ; CU – ñåðäöåâèííàÿ ÷àñòü
àêñîíåìû; cx – êîðòåêñ; DA – äèíåèíîâûå âûðîñòû; DPT – äâîéíûå ïåðèôåðè÷åñêèå ìèêðîòðóáî÷êè (äóá-
ëåòû); M – ïëàçìàòè÷åñêàÿ ìåìáðàíà; Mt – êîðòèêàëüíûå ìèêðîòðóáî÷êè; N – ÿäðî; P – öèëèíäðè÷åñêèé
ïåðèàêñîíåìàëüíûé “ôóòëÿð”; s – “ñïèöû”; ñîåäèíÿþùèå ïåðèôåðè÷åñêèå äóáëåòû ñ ñåðäöåâèííîé ÷àñòüþ
àêñîíåìû; sf A – ìèêðîòðóáî÷êà “À” â äóáëåòå; sf B – ìèêðîòðóáî÷êà “Â” â äóáëåòå.
Study of Spermatozoa of the Cestode Dilepis undula… 95
Main structural components of the sperm cell are: (1) plasma membrane and underlying
single row of cortical microtubules; (2) a single axoneme of the unique platyhelminth 9+˝1˝
type; (3) periaxonemal sheath composed of the crystalline-like material; (4) elongated elec-
tron-dense nucleus; (5) crested body (Mokhtar-Maamouri, Swiderski, 1975).
Smooth plasma membrane is of uniform thickness. Cortical microtubules, situated under
the plasma membrane (figs 2, 6, 7) are spiralled at variable angle (between 35—45°) to the
spermatozoon axis.
The axoneme is 0.15—0.18 μm in diameter (figs 6, 7). It contains a central core unit
(0.05—0.06 μm) and nine peripheral doublets of microtubules (figs 1, 2, 6). The central core
unit consists of an electron-dense core and two cylindrical areas around it composed of: (a)
an electron-lucent matrix, and (b) an electron-dense cortex. The peripheral doublet unit of
axoneme is composed of: (1) subfiber A or tubule A connected by spoke with the central
region through an electron-dense region which partially occlude the lumen of the tubule A;
(2) two dyneine arms attached to tubule A; and (3) subfiber B or tubule B (figs 1, 2, 6). The
spokes connecting the peripheral doublets with the central region, sometimes appear on the
cross-section with different prominence, which confirms their helical arrangement around
the central core unit. The periaxonemal sheath has striated appearance on the sections. It is
composed of crystalline-like material and consists of moderately electron-dense radial rods
separated by electron-lucent spaces of the same thickness (figs 3—6). Numerous granules of
moderately electron-dense material occur in the middle and posterior regions of the sper-
matozoa.
An electron-dense elongated nucleus forming a loose spiral around the axoneme (figs 1,
3, 5), is changing its shape and thickness, when observed on cross-sections at different levels.
It begins as a cylindrical structure, initially round on cross-sections, and progressively flattens
towards the posterior end, becoming crescent-shaped on the cross-sections (fig. 2). The pe-
riodicity of the nuclear spiral varies along the nucleus length. The nucleus occupies relatively
short part of the spermatozoon length. Externally, the spermatozoon shows near its anterior
end a single, short, helical crested body, associated with cortical microtubules.
Cross-sections through the spermatozoon, near its anterior and posterior tips, are much
smaller in diameter and contain only the axoneme surrounded by the plasma membrane
(fig. 1).
Fig. 2. A three-dimentional diagram of the nucleus containing part of the spermatozoon. Note a complex structure of
the 9+”1” axoneme of platyhelmynth type.
Ðèñ. 2. Òðåõìåðíàÿ äèàãðàììà ÿäðîñîäåðæàùåãî ó÷àñòêà ñïåðìàòîçîèäà. Âàæíî îòìåòèòü ñëîæíóþ ñòðóêòóðó
àêñîíåìû, óñòðîåííîé ïî òèïó 9+”1”, õàðàêòåðíîìó äëÿ ïëîñêèõ ÷åðâåé.
Z. Swiderski, R. V. Salamatin, V. V. Tkach 96
Discussion
An apical cone and a posterior electron-dense material, described in spermatozoa of
some other cyclophyllidean cestodes (Justine, 1998; Miquel, Marchand, 1998), were not ob-
served in two dilepidids examined so far (Swiderski, Tkach, 1996; present study); in both
cases the fully mature spermatozoa stored in the seminal receptacle, were studied.
In D. undula, only one crested body was observed, while 1 to 6 crest-like bodies were
reported in other cestodes (for references see Miquel, Marchand, 1997, 1998). The main
Figs 3—7. Details of supermatozoal ultrastructure. Figs 3—5: oblique sections through the nuclear part showing arrange-
ment of microtubiules at different angles to the axoneme, spiral pattern of nucleus and periaxonemal sheath composed
of the crystalline-like material. Figs 6—7: cross-sections through the anuclear parts. Note: a single row of cortical micro-
tubules and striated appearance of the periaxonemal sheath.
Ðèñ. 3—7. Äåòàëè óëüòðàñòðóêòóðû ñïåðìàòîçîèäà. Ðèñ. 3—5: ñðåçû ÷åðåç ÿäðîñîäåðæàùóþ ÷àñòü, äåìîíñòðè-
ðóþùèå ðàñïîëîæåíèå ìèêðîòðóáî÷åê ïîä ðàçëè÷íûì óãëîì ê àêñîíåìå, ñïèðàëüíóþ ôîðìó ÿäðà, à òàêæå
ïåðèàêñîíåìàëüíûé “ôóòëÿð”, ñîñòîÿùèé èç êðèñòàëëîïîäîáíîãî âåùåñòâà. Ðèñ. 6—7: ïîïåðå÷íûå ñðåçû ÷åðåç
áåçúÿäåðíóþ ÷àñòü. Âàæíî îòìåòèòü íàëè÷èå îäíîãî ðÿäà êîðòèêàëüíûõ ìèêðîòðóáî÷åê è èñ÷åð÷åííîñòü ïåðè-
àêñîíåìàëüíîãî “ôóòëÿðà”.
Study of Spermatozoa of the Cestode Dilepis undula… 97
difference between two dilepidids, examined so far, is the presence of the periaxonemal
sheath in D. undula (present study) and its absence in Molluscotaenia crassiscolex (Swiderski,
Tkach, 1996).
The fine structure of the spermatozoon of D. undula is similar to that described in other
cyclophyllideans (Swiderski, 1970; Euzet et al., 1981; Justine, 1991, 1995, 1998; Bâ, Mar-
chand, 1995) and is characterised by the presence of a single axoneme. This type of the
spermatozoon organisation is observed in the evolutionary more advanced groups of cesto-
des. It represents probably a result of the secondary reduction in the number of axonemes
from the ancestral 2-axoneme type which is observed in more primitive Pseudophyllidea,
Tetrarhynchidea and Proteocephalidea (Euzet et al., 1981).
Acknowledgements
The authors wish to thank Dr Yuri Kuzmin (Institute of Zoology NANU, Kyiv) for his technical assistance.
The research was carried out in the framework of the scientific co-operation between the Institute of Zoology of the
Ukrainian National Academy of Sciences and the Institute of Parasitology of the Polish Academy of Sciences. This
study was financially supported by the grant ¹ 6 PO4Ñ 01314 from the Polish Committee for Scientific Research
(KBN) and by a fellowship from the J. Mianowski Foundation (Warsaw, Poland) to R. Salamatin.
Bâ C. T., Marchand B. Spermiogenesis, spermatozoa and phyletic affinities in the Cestoda // Mémoires du Muséum
national d’Histoire naturelle. – 1995. – 166: Advances in spermatozoal phylogeny and taxonomy / Eds.
B. G. M. Jamieson, J. Ausio, J.-L. Justine. – P. 87—95.
Euzet L., Swiderski Z., Mokhtar-Maamouri F. Ultrastructure comparée du spermatozoïde des Cestodes. Relations
avec la phylogenèse // Ann. Parasitol. – 1981. – 58. – P. 247—259.
Jones A. Family Dipylidiidae // Keys to the Cestode parasites of Vertebrates / Eds. L. K. Khalil, A. Jones. – Cam-
bridge : University Press, 1994. – P. 555—558.
Justine J.-L. Phylogeny of parasitic Platyhelminthes: a critical study of synapomorphies proposed on the basis of the
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Justine J.-L. Spermatozoal ultrastructure and phylogeny in the parasitic Platyhelminthes // Mémoires du Muséum
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