A parentage study of closely related Ukrainian wine grape varieties using microsatellite markers
Four bred grapevine varieties released for commercial cultivation in Ukraine, namely ‘Antey Magarachskii’, ‘Rubinovyi Magaracha’, ‘Granatovyi Magaracha’ and ‘Rubin Golodrigi’, and their putative parental forms were genotyped using six microsatellite loci. Четыре селекционных сорта винограда Антей Ма...
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| Опубліковано в: : | Цитология и генетика |
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Інститут клітинної біології та генетичної інженерії НАН України
2010
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| Цитувати: | A parentage study of closely related Ukrainian wine grape varieties using microsatellite markers / S. Goryslavets, V. Risovanna, R. Bacilieri, J.-F. Hausman, M. Heuertz // Цитология и генетика. — 2010. — Т. 44, № 2. — С. 29-37. — Бібліогр.: 29 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1859765292714950656 |
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| author | Goryslavets, S. Risovanna, V. Bacilieri, R. Hausman, J.-F. Heuertz, M. |
| author_facet | Goryslavets, S. Risovanna, V. Bacilieri, R. Hausman, J.-F. Heuertz, M. |
| citation_txt | A parentage study of closely related Ukrainian wine grape varieties using microsatellite markers / S. Goryslavets, V. Risovanna, R. Bacilieri, J.-F. Hausman, M. Heuertz // Цитология и генетика. — 2010. — Т. 44, № 2. — С. 29-37. — Бібліогр.: 29 назв. — англ. |
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| description | Four bred grapevine varieties released for commercial cultivation in Ukraine, namely ‘Antey Magarachskii’, ‘Rubinovyi Magaracha’, ‘Granatovyi Magaracha’ and ‘Rubin Golodrigi’, and their putative parental forms were genotyped using six microsatellite loci.
Четыре селекционных сорта винограда Антей Магарачский, Рубиновый Магарача, Рубин Голодриги и Гранатовый Магарача, которые культивируются в Украине для приготовления сухих и крепленых вин, и их предполагаемые родительские формы были генотипированы с использованием шести микросателлитных локусов.
Чотири селекційних сорти винограду Антей Магарацький, Рубіновий Магарача, Рубін Голодриги та Гранатовий Магарача, що культивують в Україні для приготування сухих і десертних вин, та їх потенційні батьківські форми були генотиповані з використанням шести мікросателітних локусів.
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УДК 634.85:631.526.32:577.21(477)
S. GORYSLAVETS 1, V. RISOVANNA 1, R. BACILIERI 2,
J.�F. HAUSMAN 3, M. HEUERTZ 3, 4, 5 *
1 National Institute for Vine and Wine «Magarach»; Ampelography,
Breeding and Genetics of Grapevine, Yalta, Ukraine
E�mail: goricvet_2@rambler.ru
2 Institut National de la Recherche Agronomique, Centre INRA de
Montpellier 2 Place Viala, 34000 Montpellier, France
3 Centre de Recherche Public�Gabriel Lippmann; Environment and
Agro�Biotechnology; rue du Brill 41; L�4422 Belvaux, Luxembourg
4 Université Libre de Bruxelles; Evolutionary and Ecology cp160/12;
av. F.D. Roosevelt 50; B�1050 Brussels, Belgium
5 Centre of Forest Research CIFOR�INIA; Forest Systems and Resources;
ctra. de la Coruña km 7.5; E�28040 Madrid, Spain
A PARENTAGE STUDY OF CLOSELY
RELATED UKRAINIAN WINE GRAPE
VARIETIES USING MICROSATELLITE
MARKERS
Four bred grapevine varieties released for commercial cul�
tivation in Ukraine, namely ‘Antey Magarachskii’, ‘Rubinovyi
Magaracha’, ‘Granatovyi Magaracha’ and ‘Rubin Golodrigi’,
and their putative parental forms were genotyped using six
microsatellite loci. Genotypes were compared with breeding
records to verify genetic relationships among varieties. Results
of the analysis confirmed four of six parent�offspring relation�
ships. Results of the analysis allow to assume that genotype
‘Seyve Villard 20347’ is the direct parent of ‘Antey
Magarachskii’ instead of its grandparent. The first�studied
accession believed to be that of Granatovyi Magaracha was
identified as impurity. In order to verify the parentage of
Granatovyi Magaracha, rest accessions of that variety and its
putative parent Antey Magarachskii were additionally geno�
typed at 13 nuclear loci and at three chloroplast loci. The par�
ent�offspring relationship was confirmed, as all Granatovyi
Magaracha accessions had a common allele with the parent
variety Antey Magarachskii at each locus and the same
chlorotype A. Different Granatovyi Magaracha accessions could
have been obtained via vegetative propagation of two seedlings
which arose from one crossing.
Introduction. The south of Ukraine has a long�
standing tradition of growing table and wine grape
varieties. The latter are made into wines distin�
guished for their excellent quality. Recently, new
wine grapes produced by generative breeding are
being introduced into commercial cultivation.
This helps improving and enlarging the countryґs
wine grape assortment since such varieties have
better economical characters. Four newly�bred
promising vintage varieties are the focus of this
paper: ‘Rubin Golodrigi’, bred in 1974 and
released by the Research Company «Ampelos»;
‘Rubinovyi Magaracha’, ‘Antey Magarachskii’,
and ‘Granatovyi Magaracha’ were bred, respec�
tively, in 1928, 1971 and 1982 and released by the
National Institute for Vine and Wine «Magarach».
These varieties are highly resistant to diseases and
can be elaborated into table and dessert wines.
They have started to be widely grown in Russia and
in Ukraine. ‘Rubinovyi Magaracha’ and ‘Rubin
Golodrigi’ have juicy fruits with a flavour of the
berry of nightshade. ‘Antey Magarachskii’ has fruits
with a crisp juicy flesh distinguished for chocolate
flavours. Unlike the above�mentioned grapes, the
fruit of ‘Granatovyi Magaracha’ produces coloured
juice, enabling its use as teinturier [1].
An impressive diversity of varieties, forms and
species are involved in grapevine breeding. Their
origin is sometimes uncertain, which is why their
identification and the characterization of variety
pedigrees is an important task. The need to iden�
tify interspecific grapevine hybrids is especially
important for the south of Ukraine where grape
and wine growing dates back to Greek colonists
of antiquity and breeding activities have been
extensive since the middle of the 19th century [2].
Historically, ampelographic methods were the
only tool used to this end and relied mostly on
visual characters of the leaves, clusters and shoot
apices. Unfortunately, the potential and useful�
ness of this type of identification are restricted
due to considerable variation of the characters
and subjectivity of human estimates. Recently,
microsatellite markers (simple sequence repeats –
SSRs) have come into use to investigate genetic
diversity of grapevine [3, 4] and to establish
genetic relationships among varieties [5–8]. They
offer a number of advantages, including high
polymorphism and a co�dominant mode of
inheritance. They allow a precise molecular fin�
gerprint of grape genotypes and have proved to be
the most informative and popular type of DNA
ІSSN 0564–3783. Цитология и генетика. 2010. № 2 29
© S. GORYSLAVETS, V. RISOVANNA, R. BACILIERI,
J.�F. HAUSMAN, M. HEUERTZ, 2010
markers as concerns identification of varieties and
determination of their parentages [3, 5, 7–11].
The four varieties in the focus of this paper have
already been studied for a number of years using
conventional ampelographic characters, biochem�
ical indices and several morphometric variables
[12–14]. They constitute valuable breeding mate�
rial and have been used in numerous crossings.
‘Antey Magarachskii’ is a parent of several new
varieties, such as ‘Krasen’, ‘Pamyati Golodrigi’,
‘Safyanovyi’ and of eleven new forms. We report
here on genetic fingerprinting of the four varieties
and four putative parents of them in order to eval�
uate their parentages suggested by breeding
records.
The research was done at the Centre de
Recherche Public – Gabriel Lippmann (Luxem�
bourg) and the National Institute for Vine and
Wine «Magarach» (Yalta, Ukraine) in the frame�
work of the international project «Conservation and
Sustainable Use of Grapevine Genetic Resources
in the Caucasus and in the Northern Black Sea
Region» coordinated by Bioversity International
(formerly: IPGRI). The accessions of the variety
Granatovyi Magaracha and Antey Magarachskii
were genotyped at the Institut National de la
Recherche Agronomique (INRA, France) in the
context of a research programme supported by the
National Institute of Agricultural Research of
France (called ECO�NET).
Materials and Methods. Plant material and DNA
extraction. The plant material used for genetic
characterization in this study are four wine grape
varieties with black berries, namely ‘Antey Maga�
rachskii’, ‘Rubinovyi Magaracha’, ‘Granatovyi Ma�
garacha’ and ‘Rubin Golodrigi’and some of their
putative parents, namely the Georgian autochtho�
nous variety ‘Saperavi’, the old Moldavian variety
‘Maiskii Chernyi’, the widely grown French vari�
ety ‘Cabernet Sauvignon’ and the Vitis interspecif�
ic hybrid Seyve Villard 20347 (Table 1). ‘Rubin
Golodrigi’, ‘Antey Magarachskii’ and ‘Granatovyi
Magaracha’ possess multiple resistances to pests and
diseases [15, 16]. These varieties are of complex
interspecific origin since Vitis interspecific hybrids
(‘Seyve Villard 20347’, ‘Magarach 6–68–27’, ‘Ma�
garach 85–64–16’, etc.) are reported to have been
used as their parents at different stages of the
breeding process (Figure). The variety ‘Saperavi’ is
a teinturier with a lot of pigments in its skins,
which encourages its use as a colour�enhancing
element in white and red wine technologies [17,
18]. Unfortunately, all putative parents could not
be analysed since some of them have been lost. For
DNA extraction young shoots without symptoms
of pathology were collected from accessions of the
test varieties growing in the collection of the Institute
«Magarach» (experiment farm in the village of
Vilino, Ukraine). The shoots were frozen in liquid
nitrogen and stored at a temperature of –86 °С.
DNA was extracted from the leaf tissue follow�
ing the method of Lefort and Roubelakis�
Angelakis [19].
In order to standardize genotyping results
according to the «European Vitis Database», we
additionally analysed 28 varieties [20] for which
genotypes were published in This et al. [21].
Cuttings from accessions of these varieties were
kindly provided by Dr. Didier Vares from the Institut
National de la Recherche Agronomique (INRA),
Vassal, France. Buds were dissected out and DNA
was extracted using the NucleoSpin Plant kit
(Macherey Nagel). Accessions of ‘Cabernet Sau�
vignon’ from both the collection of the «Magarach»
Institute in Ukraine and from the INRA Vassal
collection in France have been included in the
analysis.
Microsatellite analysis. We chose six
microsatellite primers widely used for genetic fin�
gerprinting of grape varieties and recommended
by «Bioversity International» and the «European
Vitis Database» [21]: VVS2 [22], VVMD5,
VVMD7 [23, 24], VVMD27 [24], VrZAG62 and
VrZAG79 [25]. PCRs (10 μl) were performed
using 0.25 U of FideliTaq polymerase (GE
Healthcare), 1X reaction buffer (10 mM Tris�HCl
(pH 8.6), 50 mM KCl, 1.5 mM MgCl2) (GE
Healthcare), 2 mM of each dNTP, 0.4 μM of each
primer and 1 μl of DNA extract diluted 40 times
than give concentrations. PCR conditions were as
follows: 95 °С for 4 min, 40 cycles of 94 °С for
30 sec, 52 °С for 20 sec, 72 °С for 1 min, followed
by a final extension for 7 min at 72 °С and cooling
to 4 °С.
The forward primer of each microsatellite locus
was labelled with a fluorescent dye (6�FAM, PET,
NED or VIC, Table 2) to visualize PCR amplifica�
tion products from all loci in the same analysis run
on an automated monocapillary sequencer (ABI
Prism 310, Applied Biosystems). Allele sizes were
ISSN 0564–3783. Цитология и генетика. 2010. № 230
S. Goryslavets, V. Risovanna, R. Bacilieri, J.�F. Hausman, M. Heuertz
ІSSN 0564–3783. Цитология и генетика. 2010. № 2 31
A parentage study of closely related Ukrainian wine grape varieties
Table 1
Grape cultivars included in this study and parentage information on them on basis breeding reports
Genus
Vitis
Vitis
Vitis
Vitis
Vitis
Vitis
Vitis
Vitis
Species
Hybrid *
Hybrid
vinifera L.
Hybrid
vinifera L.
vinifera L.
Interspeci�
fic cross
vinifera L.
Antey Maga�
rachskii
Granatovyi
Magaracha
Cabernet
Sauvignon
Rubin Golo�
drigi
Rubinovyi
Magaracha
Saperavi
Seyve Villard
20347
Maiskii
Chernyi
Magarach
70�71�52
Magarach
77�81�3
Magarach
15�74�29
Magarach
56
Perle Noire
German
black
Variety
name
Possible
synonyms
Parentage
(A � B)
Use
(W, Wine
grape;
T, Table
grape)
T, W
W
W
W
W
W
T, W
W
Rubinovyi Maga�
racha � Magarach
85�64�16
Antey Magarachskii �
� Magarach
11�57�130
Cabernet franc � Sau�
vignon blanc
Rubinovyi Magara�
cha � Magarach
6�68�27
Cabernet Sauvignon �
� Saperavi
Unknown
V. vinifera � Seyve
Villard 12358
Unknown
Breeding
station
NIVW «Maga�
rach» **
NIVW «Maga�
rach»
Old French va�
riety
Research Com�
pany
«Ampelos»
NIVW «Maga�
rach»
Autochtonous
Georgian vari�
ety
Old Moldavian
variety
Authors
P. Golodriga, V. Usatov, L.
Troshin, Yu. Mal’chikov,
N. Dubovenco
V. Usatov, L. Kireeva, P. Go�
lodriga, L. Troshin, V. Vo�
linkin, V. Klimenko, N. Ole�
ynikov, Yu. Mal’chikov
P. Golodriga, M. Kostik,
V. Yurchenko
N. Paponov, V. Zotov, P. Tsa�
rev, P. Golodriga
Seyve Villard
* Accessions are qualified as hybrids if they have a complex genetic background, involving some non�vinifera varieties. **NIVW:
National Institute for Vine and Wine.
VVS2
dye:6�FAM *,
stdized to
n = 123 **
VVMD5
dye: PET
stdized to
n = 222
VVMD7
dye:6�FAM
stdized to
n = 232
VVMD27
dye: NED
stdized to
n = 175
Variety
Cabernet Sauvignon
Rubinovyi Magaracha
Saperavi
Antey Magarachskii
Granatovyi Magaracha N1
Maiskii Chernyi
Rubin Golodrigi
Seyve Villard 20347
240 240
240 240
240 240
240 250
240 240
240 250
240 252
250 252
232 240
232 240
224 240
226 232
234 234
226 234
232 240
226 232
139 151
133 151
133 145
133 145
133 145
143 143
133 151
145 149
175 189
0 0
189 192
179 189
0 0
179 189
192 194
179 189
188 194
188 200
188 200
186 188
196 196
188 194
194 200
186 194
248 248
248 262
244 262
248 256
244 244
238 244
246 248
256 262
VrZAG62
dye: VIC
stdized to
n = 174
VrZAG79
dye: NED
stdized to
n = 238
Table 2
Allele compositions of eight grape genotypes analysed with 6 microsatellite loci (VVS2, VVMD5, VVMD7, VVMD27,
VrZAG62 and VrZAG79) standardized to allele sizes published in This et al. [21]. Missing data are coded with «0»
* The fluorescent dyes used to label forward primers of each locus were 6�FAM, PET, NED or VIC. ** Allele sizes are expressed
in base pairs (bp). *** «Stdized to n = 123» means that the length of the shortest PCR product discovered at that particular
by This et al. [21] was 123 bp; it is the allele size to which PCR fragment lengths in this study were standardized.
determined as PCR product lengths by compari�
son with an internal size standard (GeneScanTM
500 LIZ�) using GeneMapper 3.0 software (both
Applied Biosystems). They were recorded in base
pairs (bp) with a two decimals precision. The com�
parison of the allele sizes obtained for reference
varieties to the This et al. [21] data allowed stan�
dardization of allele size of the complete data set in
order to achieve compatibility with the format of
the European Vitis Database.
In order to verify the parentage of Granatovyi
Magaracha, two others accessions of that variety
(Granatovyi Magaracha N2 and N3) and its par�
ent Antey Magarachskii were additionally ana�
lyzed for 13 nuclear loci and for three chloroplast
loci at the Institut National de la Recherche
Agro�nomique (INRA, France) on an automated
capillary sequencer (ABI Prism 3130X, Applied
Biosystems).
Data analysis. Genetic diversity statistics of
each locus in the total number of eight target
genotypes were computed using the SPAGeDi
version 1.2 software [26]. Diversity was estimated
as number of alleles and expected heterozygosity
(gene diversity). First degree parentage relation�
ships (parent�offspring relationships) were iden�
tified using GIMLET version 1.3.3 [27]. Absolute
coefficients of molecular coancestry (kinship)
between pairs of varieties were computed as aver�
ages over loci according to Lynch and Walsh [28].
The coefficient of coancestry between two vari�
eties is defined as the probability that two ran�
domly drawn genes at a locus, one in each variety,
are identical by descent. In our estimation, we
assume that genes are identical by descent if they
show the same PCR product length in
microsatellite analysis, i.e. if they are identical in
state. We hence do not account for possible
inbreeding of varieties. The reason for this is that
the base population of the varieties from which
inbreeding coefficients could be computed is
unknown.
Results and Discussion. Genotypes of eight
focal (Table 2) and twenty�eight reference varieties
ISSN 0564–3783. Цитология и генетика. 2010. № 232
S. Goryslavets, V. Risovanna, R. Bacilieri, J.�F. Hausman, M. Heuertz
Crossing records of the focal varieties and their parental forms. Varieties subjected to genetic analysis are marked with an
asterisk (*). Relationships suggested by genetic fingerprinting are depicted with bold arrows. The dotted arrow represents a
new relationship suggested by genetic data. The parent�offspring relationship between ‘Antey Magarachskii’ and ‘Granatovyi
Magaracha N1’ suggested from the breeding design was not confirmed with genetic data (bold bars)
[20] were obtained at six microsatellite loci. The
accession of ‘Cabernet Sauvignon’ from the col�
lections of the Institute «Magarach» was found to
be genetically identical to that of INRA at all six
microsatellites. No clear amplification product
could be obtained in ‘Rubinovyi Magaracha’ and
‘Granatovyi Magaracha’ at locus VVMD27 despite
repetitive PCRs. Non�amplification might be due
to a mutation in one of the PCR primer annealing
sites, causing a «null allele» [29]. The missing data
are coded with «0». Genetic diversity statistics of
microsatellite loci are summarized in Table 3. The
total number of alleles observed at the six loci was
30. The lowest polymorphism was observed at
locus VVMD7 with 3 alleles and a level of het�
erozygosity equal to HE = 0.508, the highest poly�
morphism occurred at VVS2 and VrZAG79 with
each 6 alleles and heterozygosity equal to HE =
= 0.833 (Table 3).
Putative first degree parentage relationships
among the focal varieties were identified using
GIMLET v1.3.3 software and compared with the
available crossing records (Figure). ‘Rubinovy
Magaracha’ was found to be a compatible off�
spring of a cross between ‘Cabernet Sauvignon’
and ‘Saperavi’, sharing 50 % of its allele compo�
sition with each of its putative parents. Similarly,
the genetic data confirmed that both ‘Rubin
Golodrigi’ and ‘Antey Magarachskii’ were com�
patible offspring of ‘Rubinovyi Magaracha’ used
as a female parent, as suggested from the breeding
records. Furthermore, ‘Antey Magarachskii’ was a
compatible offspring of the parent pair ‘Rubi�
novyi Magaracha’ and ‘Seyve Villard 20347’. This
suggests that ‘Seyve Villard 20347’ could be the
direct male parent rather than a grandparent to
‘Antey Magarachskii’. ‘Antey Magarachskii’ is
known to be close to its female parental line,
especially to the cultivar ‘Cabernet Sauvignon’,
as concerns morphological characteristics of the
leaves and clusters and the taste of the berries. On
the other hand good resistance to downy mildew,
powdery mildew and adaptation to low tempera�
tures is inherited from its male parent, ‘Seyve
Villard 20347’. Besides, ‘Antey Magarachskii’
and ‘Seyve Villard 20347’ both have two uses of
the fruit, either for fresh consumption or vine
making [17].
The genetic data showed that the accession of
‘Granatovyi Magaracha’ does not share one
allele at each locus with its supposed male parent
‘Antey Magarachskii’; as a matter of fact, three
loci (VVMD5, VrZAG62 and VrZAG79) do not
support a parent�offspring relationship between
ІSSN 0564–3783. Цитология и генетика. 2010. № 2 33
A parentage study of closely related Ukrainian wine grape varieties
Table 3
Characterization of microsatellite loci of the eight grape
genotypes described in this paper. HE: expected
heterozygosity or gene diversity
Locus
VVS2
VVMD5
VVMD7
VVMD27
VrZAG62
VrZAG79
16
16
16
12
16
16
6
5
3
5
5
6
0.833
0.817
0.508
0.788
0.825
0.833
142.0
232.8
243.4
186.3
192.5
249.9
49.6
28.7
27
40.8
25.9
55.2
Number
of
defined
Number
of alleles
HE
Mean
allele
size
Variance
of allele
size
Table 4
Matrix of coancestry coefficients between pairs of varieties. In the absence of inbreeding of the parents,
a coancestry coefficient of 0.25 is expected between parent and offspring. Overall high coancestry coefficients
highlight the high relatedness of varieties
Cabernet
Sauvignon
Saperavi
Rubinovyi
Magaracha
Antey Ma�
garachskii
Granatovyi
Magaracha
Rubin
Golodrigi
Seyve
Villard
Maiskii
Chernyi
Variety
Cabernet Sauvignon
Saperavi
Rubinovyi Magaracha
Antey Magarachskii
Granatovyi Magaracha
Rubin Golodrigi
Seyve Villard 20347
Maiskii Chernyi
0.667
0.292
0.500
0.292
0.200
0.333
0.125
0.208
0.583
0.450
0.250
0.400
0.250
0.125
0.208
0.600
0.300
0.250
0.400
0.100
0.150
0.500
0.200
0.167
0.333
0.250
0.900
0.150
0.050
0.300
0.500
0.125
0.083
0.500
0.208 0.583
these varieties. Contrary to other varieties which
were heterozygous at four loci at least, ‘Gra�
natovyi Magaracha’ was heterozygous only for
VVS2 and homozygous for VVMD5, VVMD7,
VrZAG62 and VrZAG79. This variety showed
therefore the highest coefficient of coancestry with
itself (0.90, Table 4). The coancestry coefficient
technically corresponds to the inbreeding coeffi�
cient of the variety’s selfed offspring. Moreover,
‘Granatovyi Magaracha’ also displayed the lowest
adaptive variation when morphogenetic responses
of the test varieties were studied in vitro [13]. A
possible reason for this may be that the variety
results from a multi�step breeding process includ�
ing self�fertilisation.
However, a low degree of allele diversity at neu�
tral loci such as microsatellites is not necessarily
associated with a low level of adaptive variation
and may be merely coincidental. ‘Granatovyi
Magaracha’ contained alleles that were absent
from other genotypes of its putative paternal line
(Table 2), namely n + 12 (234) at VVMD5, n + 22
(196) at VrZAG62 and n + 6 (244) at VrZAG79.
Two of these alleles (234 at VVMD5 and 244 at
VrZAG79) were detected in ‘Maiskii Chernyi’, a
putative maternal great�grandparent of ‘Grana�
tovyi Magaracha’, indicating that they could have
been inherited from the putative female line.
Unfortunately, the hybrid form ‘Magarach
11–57–130’ which is the supposed female parent
of ‘Granatovyi Magaracha’ has been lost, so that it
has become impossible to verify this suggestion.
Our results indicate that the origin of ‘Granatovyi
Magaracha’ is so far not clear and needs further
investigation. In this connection, the remaining
accessions of the variety Granatovyi Magaracha
(N2 and N3) growing in the collection of the
Institute for Vine and Wine «Magarach» were
genotyped at the Institut National de la Recherche
Agronomique (INRA, France).
Their relationships with each other and the par�
ent�offspring relationship with the putative parent
variety Antey Magarachskii were analyzed for
13 nuclear loci (VVIn16, VIp60, VVIv67,
VMC4f3, VVIb01, VVMD7, VVMD21, VVMD28,
VVIq52, VVIv37, VVS2, VrZAG62 and VrZAG79)
and for three chloroplast microsatellite loci
(CCMP3, CCMP5 and CCMP10). As a result, it
was found that the genotypes of the two accessions
were identical to that of Antey Magarachskii at loci
VVMD21, VVIq52 and VVIv37 while each acces�
sion shared one common allele with Antey
Magarachskii at the remaining ten loci (Table 5).
Thus, the percentage of shared alleles in the geno�
types of the two accessions of Granatovyi
Magaracha (0.7) indicates their close relatedness.
They could have been obtained via vegetative
propagation of two seedlings which arose from one
crossing.
This is compatible with results of analysis of the
accessions for chloroplast microsatellite loci
CCMP3, CCMP5 and CCMP10. Chlorotype D
ISSN 0564–3783. Цитология и генетика. 2010. № 234
S. Goryslavets, V. Risovanna, R. Bacilieri, J.�F. Hausman, M. Heuertz
Table 5
Allele compositions of the parent variety Antey Magarachskii and two asseccions of Granatoviy Magaracha analysed with
13 nuclear loci (VVIn16, VIp60, VVIv67, VVMD7, VVMD21, VMC4f3, VVIb01, VVMD28, VVIq52, VVIv37, VVS2,
VrZAG62 and VrZAG79) and for three chloroplast microsatellite loci (CCMP3, CCMP5 and CCMP10)
Accession
name VVIv67VVIv37VVIq52VVIp60VVIn16 VMD21 VMC4f3 VVIb01 VVMD28
VVMD7 VVS2 VrZAG62 VrZAG79 CCMP3 CCMP5 CCMP10 Haplotip
Antey Magarachskii
Granatovyi Magaracha N2
Granatovyi Magaracha N3
Accession
name
Antey Magarachskii
Granatovyi Magaracha N2
Granatovyi Magaracha N3
147 151
147 151
147 149
79 79
79 79
79 79
149 159
149 159
149 159
334 368
334 353
334 368
247 247
247 247
247 247
171 171
171 171
171 202
290 294
294 294
290 294
235 235
235 235
235 245
240 250
240 244
240 244
133 145
133 133
133 133
186 188
188 188
188 192
248 256
244 256
244 256
107
106
106
104
105
105
115
114
114
D
A
A
315 315
315 319
315 319
(107/104/115) was identified in the parent form
Antey Magarachskii.
The two accessions of Granatovyi Magaracha
had the same chlorotype А (106/105/114), which
suggest that they descend from one female form as
chloroplast inheritance is matrilinear. Thus, analy�
sis of the accessions of Granatovyi Magaracha
confirmed the parent�offspring relationship of the
male parent Antey Magarachskii and the former
variety.
Overall, fairly high coefficients of coancestry
were detected between the varieties investigated
(Table 4), considering that many values are higher
than 0.25, which is the average expectation for a
parent�offspring relationship ignoring inbreeding
[28]. However, despite the overall high related�
ness, each variety was found to have a unique SSR
profile.
The results from this study highlight the use�
fulness of microsatellites in parentage analysis
and for verifying pedigree information in
grapevine, as has been observed by other authors
[6–8].
Genetic data in our study were compatible
with five out of six parent�offspring relationships
tested (Figure), and they all included ‘Rubinovyi
Magaracha’. However, to safely confirm these
relationships, data at 30 to 50 micro� satellites
would be necessary [24]. Genetic data allowed to
identify first�studied accession of Granatovyi
Magaracha as impurity and to suggest ‘Seyve
Villard 20347’ as the father of ‘Antey
Magarachskii’.
We are grateful to Dr. V. Klimenko for valuable
comments on issues referring to breeding, Dr. Amine
Memetova, Ms. Valentina Petrashko and Mr. Sergey
Makeiev for their help in collecting plant materials.
We thank Dr. Didier Vares from INRA Vassal for
providing cuttings of reference varieties, Dr. Patrice
This for offer an opportunity to work in the Diversity
and Genome laboratory from Centre INRA de
Montpellier and Dr. Valerie Laukou from Centre
INRA de Montpellier for consultations in microssatel�
lite analysis and aleles standartization.
This work was funded by the Luxembourg
Ministry of Finance, by Bioversity International and
by the Ministry of Foreign Affairs of France (ECO�
NET program).
С. Гориславец, В.Рисованная,
R. Bacilieri, J.�F. Hausman, M. Heuertz
ИЗУЧЕНИЕ РОДОСЛОВНОЙ
БЛИЗКОРОДСТВЕННЫХ УКРАИНСКИХ
ТЕХНИЧЕСКИХ СОРТОВ ВИНОГРАДА
С ИСПОЛЬЗОВАНИЕМ МИКРОСАТЕЛЛИТНЫХ
МАРКЕРОВ
Четыре селекционных сорта винограда Антей Ма�
гарачский, Рубиновый Магарача, Рубин Голодриги
и Гранатовый Магарача, которые культивируются
в Украине для приготовления сухих и крепленых вин,
и их предполагаемые родительские формы были гено�
типированы с использованием шести микросателлит�
ных локусов. Для оценки генетических взаимоотно�
шений полученные генотипы были проанализирова�
ны на соответствие их селекционной схеме. Результа�
ты анализа подтвердили четыре связи родитель–пото�
мок из шести. Результаты проведенного анализа по�
зволяют предположить, что генотип Сейв Виллард
20347 является прямым родителем Антея Магарачско�
го, а не его прародителем. Проанализированный об�
разец сорта Гранатовый Магарача не соответствовал
отношению родитель–потомок и был идентифициро�
ван как примесь. Чтобы уточнить происхождение
упомянутого сорта, остальные два образца были до�
полнительно проанализированы по 13 ядерным и
трем хлоропластным локусам. Анализ наследования
показал, что изученные образцы имели общий мате�
ринский хлоротип, а в каждом из 13 локусов имели
общий аллель с отцовским сортом Антей Магарач�
ский, что соответствует генотипам сеянцев от одного
скрещивания.
С. Гориславець, В.Рісованна,
R. Bacilieri, J.�F. Hausman, M. Heuertz
ВИВЧЕННЯ РОДОВОДУ
БЛИЗЬКОСПОРІДНЕНИХ УКРАЇНСЬКИХ
ТЕХНІЧНИХ СОРТІВ ВИНОГРАДУ
З ВИКОРИСТАННЯМ МІКРОСАТЕЛІТНИХ
МАРКЕРІВ
Чотири селекційних сорти винограду Антей Мага�
рацький, Рубіновий Магарача, Рубін Голодриги та
Гранатовий Магарача, що культивують в Україні для
приготування сухих і десертних вин, та їх потенційні
батьківські форми були генотиповані з використан�
ням шести мікросателітних локусів. Для оцінки гене�
тичних взаємовідносин отримані генотипи були про�
аналізовані на їх відповідність селекційній схемі. Ре�
зультати аналізу підтвердили чотири зв’язки батько –
нащадок із шести. Результати проведеного аналізу до�
зволяють припустити, що генотип Сейв Віллард 20347
є прямий батько Антея Магарацького, а не його пра�
батько. Проаналізований зразок сорту Гранатовий Ма�
ІSSN 0564–3783. Цитология и генетика. 2010. № 2 35
A parentage study of closely related Ukrainian wine grape varieties
гарача не відповідав відношенню батько – нащадок
і був ідентифікований як домішка. Для уточнення по�
ходження Гранатовий Магарача решта зразків була до�
датково проаналізована за 13 ядерними і трьома хло�
ропластними локусами. Аналіз спадкування показав,
що вивчені зразки мали материнський хлоротип, а в
кожному із 13 локусів мали спільний алель із бать�
ківським сортом Антей Магарацький, що відповідає
генотипам сіянців від одного схрещування.
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A parentage study of closely related Ukrainian wine grape cultivars
|
| id | nasplib_isofts_kiev_ua-123456789-66706 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 0564-3783 |
| language | English |
| last_indexed | 2025-12-02T05:25:47Z |
| publishDate | 2010 |
| publisher | Інститут клітинної біології та генетичної інженерії НАН України |
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| spelling | Goryslavets, S. Risovanna, V. Bacilieri, R. Hausman, J.-F. Heuertz, M. 2014-07-20T17:54:00Z 2014-07-20T17:54:00Z 2010 A parentage study of closely related Ukrainian wine grape varieties using microsatellite markers / S. Goryslavets, V. Risovanna, R. Bacilieri, J.-F. Hausman, M. Heuertz // Цитология и генетика. — 2010. — Т. 44, № 2. — С. 29-37. — Бібліогр.: 29 назв. — англ. 0564-3783 https://nasplib.isofts.kiev.ua/handle/123456789/66706 634.85:631.526.32:577.21(477) Four bred grapevine varieties released for commercial cultivation in Ukraine, namely ‘Antey Magarachskii’, ‘Rubinovyi Magaracha’, ‘Granatovyi Magaracha’ and ‘Rubin Golodrigi’, and their putative parental forms were genotyped using six microsatellite loci. Четыре селекционных сорта винограда Антей Магарачский, Рубиновый Магарача, Рубин Голодриги и Гранатовый Магарача, которые культивируются в Украине для приготовления сухих и крепленых вин, и их предполагаемые родительские формы были генотипированы с использованием шести микросателлитных локусов. Чотири селекційних сорти винограду Антей Магарацький, Рубіновий Магарача, Рубін Голодриги та Гранатовий Магарача, що культивують в Україні для приготування сухих і десертних вин, та їх потенційні батьківські форми були генотиповані з використанням шести мікросателітних локусів. We are grateful to Dr. V. Klimenko for valuable comments on issues referring to breeding, Dr. Amine Memetova, Ms. Valentina Petrashko and Mr. Sergey Makeiev for their help in collecting plant materials. We thank Dr. Didier Vares from INRA Vassal for providing cuttings of reference varieties, Dr. Patrice This for offer an opportunity to work in the Diversity and Genome laboratory from Centre INRA de Montpellier and Dr. Valerie Laukou from Centre INRA de Montpellier for consultations in microssatellite analysis and aleles standartization. This work was funded by the Luxembourg Ministry of Finance, by Bioversity International and by the Ministry of Foreign Affairs of France (ECONET program). en Інститут клітинної біології та генетичної інженерії НАН України Цитология и генетика Оригинальные работы A parentage study of closely related Ukrainian wine grape varieties using microsatellite markers Изучение родословной близкородственных украинских технических сортов винограда с использованием микросателлитных маркеров Вивчення родоводу близькоспоріднених українських технічних сортів винограду з використанням мікросателітних маркерів Article published earlier |
| spellingShingle | A parentage study of closely related Ukrainian wine grape varieties using microsatellite markers Goryslavets, S. Risovanna, V. Bacilieri, R. Hausman, J.-F. Heuertz, M. Оригинальные работы |
| title | A parentage study of closely related Ukrainian wine grape varieties using microsatellite markers |
| title_alt | Изучение родословной близкородственных украинских технических сортов винограда с использованием микросателлитных маркеров Вивчення родоводу близькоспоріднених українських технічних сортів винограду з використанням мікросателітних маркерів |
| title_full | A parentage study of closely related Ukrainian wine grape varieties using microsatellite markers |
| title_fullStr | A parentage study of closely related Ukrainian wine grape varieties using microsatellite markers |
| title_full_unstemmed | A parentage study of closely related Ukrainian wine grape varieties using microsatellite markers |
| title_short | A parentage study of closely related Ukrainian wine grape varieties using microsatellite markers |
| title_sort | parentage study of closely related ukrainian wine grape varieties using microsatellite markers |
| topic | Оригинальные работы |
| topic_facet | Оригинальные работы |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/66706 |
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