The influence of some environmental factors on cytological and biometric parameters and chlorophyll content of Deschampsia antarctica Desv. in the maritime Antarctic
Under the environmental conditions of the Point Thomas Oasis (King George Island, the South Shetland Islands), we studied the influence of month-long artificial treatment with fresh water, salt water, and guano solution on the biometric characteristics, chlorophyll content, as well as the nuclear ar...
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Інститут клітинної біології та генетичної інженерії НАН України
2011
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| Cite this: | The influence of some environmental factors on cytological and biometric parameters and chlorophyll content of Deschampsia antarctica Desv. in the maritime Antarctic / І.Yu. Parnikoza, P. Loro, N.Yu. Miryuta, V.А. Kunakh, I.А. Kozeretska // Цитология и генетика. — 2011. — Т. 45, № 3. — С. 43-50. — Бібліогр.: 32 назв. — англ. |
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| author | Parnikoza, I.Yu. Loro, P. Miryuta, N.Yu. Kunakh, V.A. Kozeretska, I.A. |
| author_facet | Parnikoza, I.Yu. Loro, P. Miryuta, N.Yu. Kunakh, V.A. Kozeretska, I.A. |
| citation_txt | The influence of some environmental factors on cytological and biometric parameters and chlorophyll content of Deschampsia antarctica Desv. in the maritime Antarctic / І.Yu. Parnikoza, P. Loro, N.Yu. Miryuta, V.А. Kunakh, I.А. Kozeretska // Цитология и генетика. — 2011. — Т. 45, № 3. — С. 43-50. — Бібліогр.: 32 назв. — англ. |
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| description | Under the environmental conditions of the Point Thomas Oasis (King George Island, the South Shetland Islands), we studied the influence of month-long artificial treatment with fresh water, salt water, and guano solution on the biometric characteristics, chlorophyll content, as well as the nuclear area of leaf parenchymal cells and nuclear DNA content, in a maritime Antarctic aboriginal plant Deschampsia antarctica. The modeled factors induced an increase in the generative shoot height and the length of the largest leaf, but did not influence the number of flowers. Treatment with guano caused an increase in the chlorophyll a and b contents, while fresh water treatment only led to some increase in chlorophyll a. Fluctuations of physiologically significant traits, such as the nuclear area and DNA content in the leaf parenchyma cells of D. antarctica, have been traced under the influence of the studied factors. Understanding of the hierarchy of influence of these factors as well as and sensitivity of plants of this species to external agents require further investigation.
В условиях оазиса Поинт Томаса (остров Короля Георга, Южные Шетлендские острова) было изучено влияние искусственной обработки в течение месяца пресной, морской водой и раствором гуано на биометрические показатели, содержание хлорофиллов, а также площадь ядра паренхимных клеток листка и содержание ядерной ДНК в них у растений аборигенного вида Прибрежной Антарктики – antarctica. Искусственное влияние факторов окружающей среды вызвало увеличение высоты генеративного побега и длины наибольшего листа, но не влияло на количество цветков. Обработка раствором гуано увеличивала содержание хлорофиллов a и b, а пресной водой – только содержание хлорофилла a. Выявлены признаки флуктуации физиологически значимых параметров: площади ядра и содержания ДНК клеток паренхимы листа D. antarctica, для выяснения иерархии которых, а также степени чувствительности к внешним факторам требуются более детальные исследования.
В умовах оази Поінт Томаса (острів Короля Георга, Південні Шетлендські острови) було вивчено вплив штучної обробки протягом місяця прісною, морською водою та розчином гуано на біометричні показники, вміст хлорофілів, а також площу ядра паренхімних клітин листка та вміст ядерної ДНК в них, у рослин аборигенного виду Прибережної Антарктики – antarctica. Штучний вплив факторів довкілля викликав збільшення висоти генеративного пагону та довжини найбільшого листка, але не впливав на кількість квіток. Обробка розчином гуано збільшувала вміст хлорофілів a та b, а прісною водою – тільки вміст хлорофілу a. Виявлено ознаки флуктуації фізіологічно-значущих параметрів: площі ядра та вмісту ДНК клітин паренхіми листка D. antarctica, з’ясування ієрархії яких, а також ступеню чутливості до зовнішніх факторів потребує детальнішого дослідження.
|
| first_indexed | 2025-12-07T17:44:25Z |
| format | Article |
| fulltext |
УДК 292.3+551.8+582.35/.99+575.17
І.Yu. PARNIKOZA 1, P. LORO 2, N.Yu. MIRYUTA 1,
V.А. KUNAKH 1, I.А. KOZERETSKA 3
1 Institute of Molecular Biology and Genetics of the National Academy
of Sciences of Ukraine, Kyiv,
E�mail: kunakh@imbg.org.ua, parnikoza@gmail.com
2 University of Warmia and Mazury Department of Botany and Plant
Protection, Poland, Plac Lodzki 1, Olsztyn 10–727
3 Taras Shevchenko Kyiv National University
THE INFLUENCE OF SOME
ENVIRONMENTAL FACTORS
ON CYTOLOGICAL AND BIOMETRIC
PARAMETERS AND CHLOROPHYLL
CONTENT OF DESCHAMPSIA
ANTARCTICA DESV. IN THE MARITIME
ANTARCTIC
Under the environmental conditions of the Point Thomas
Oasis (King George Island, the South Shetland Islands), we
studied the influence of month�long artificial treatment with
fresh water, salt water, and guano solution on the biometric
characteristics, chlorophyll content, as well as the nuclear
area of leaf parenchymal cells and nuclear DNA content, in a
maritime Antarctic aboriginal plant Deschampsia antarctica.
The modeled factors induced an increase in the generative
shoot height and the length of the largest leaf, but did not
influence the number of flowers. Treatment with guano caused
an increase in the chlorophyll a and b contents, while fresh
water treatment only led to some increase in chlorophyll a.
Fluctuations of physiologically significant traits, such as the
nuclear area and DNA content in the leaf parenchyma cells of
D. antarctica, have been traced under the influence of the
studied factors. Understanding of the hierarchy of influence of
these factors as well as and sensitivity of plants of this species
to external agents require further investigation.
Introduction. Antarctic is a region currently
experiencing the most profound climate alteration
on the planet. Its ice caps continue to melt and
new areas are being released from ice as a conse�
quence of warming in the maritime Antarctic [1,
2]. Under such circumstances, the advance and
changes in the composition of plant communities
may serve as a reliable indicator of the progress of
these processes in different regions of the maritime
Antarctic [3]. Among these communities, first and
foremost attention should be paid to the formation
of cenoses of the Antarctic herb tundra composed
of two species of vascular plants: Antarctic hair�
grass (Deschampsia antarctica Desv.) and Antarctic
pearlworth (Colobanthus quitensis (Kunth.) Bartl.)
that are probably most sensitive to warming, as
well as of different mosses and macroalgae [4–6].
For using plant communities as indicators of cli�
matic changes it is essential to study the variability
of their composition, as well as the parameters of
plant edificators in relation to the ecological con�
ditions of their habitats. In this respect, the
dependence of the composition of plant commu�
nities on the distance from guano sources is the
most thoroughly studied topic so far [7–9].
Besides, a series of publications exists on the
dependence of the Antarctic herb tundra forma�
tion on the basic natural gradients, such as the dis�
tance gradient from ocean coast to the brink of
icecaps based, first of all, on differences in mois�
ture [10, 11]. In these studies, most attention has
been paid to the influence of ecological gradients
on the composition of multispecies cenoses. The
impact of the factors directly on certain species,
namely the Antarctic herb tundra formation edifi�
cator D. antarctica remains poorly understood.
In this respect, there are only a few specific
observations of the effects of direct influence of
ecological factors on individuals of D. antarctica in
situ which demonstrate a general growth rate
increase in the Antarctic hairgrass in places where
extra organic matter is available [12, 13]. Yet the
mechanism of the reaction of an individual plant
to changes in humidity or extra organics inflow
may be realized in a variety of ways. The primary
manifestation is in variability of the plant biomet�
rics. Besides, several authors demonstrated a rapid
chlorophyll content response to both internal and
external factors [7, 14–16]. Under extreme condi�
tions, polyploidization in separate tissues and in
the whole plant organism has been reported
[17–19]. In our previous research we also demon�
ІSSN 0564–3783. Цитология и генетика. 2011. № 3 43
© І.Yu. PARNIKOZA , P. LORO, N.Yu. MIRYUTA ,
V.А. KUNAKH, I.А. KOZERETSKA, 2011
strated a reaction of nucleus square metrics and its
DNA content to different growth conditions in D.
antarctica growing in the Argentine Islands region
[20, 21]. In view of this, the objective of the pres�
ent research was to study the influence of modeled
natural factors on some biometric, physiological,
and cytogenetic parameters of D. antarctica plants
in situ.
Materials and methods. Study areas. The exper�
iment was conducted during the 30th Polish and
the 10th Ukrainian expeditions (11.09.2005–
02.09.2006) in the Point Thomas Oasis near the
Polish Antarctic H. Arctowski Station on King
George Island of the South Shetland Islands, mar�
itime Antarctic. A plot was chosen with a relative�
ly homogenous cover of D. antarctica (Fig. 1).
Coordinates of the study area were determined
using GPS (Garmin eTrex H).
Characteristics of the area with the studied
plots are as follows: the Uplaz slopes region on the
bank of an ice stream, S 62°09.735', W 58°28.253',
20 m above sea level, inclination 5–10°, 350 m
away from the sea coast, mosaic inflow of guano
from birds. Four 1 m2 plots were designated within
the chosen area.
Experimental setup. The first plot was irrigated
with fresh water, plot 2 – with salt water, and plot
3 – with a guano solution. Plot 4 served as a con�
trol. The fresh water was taken from the nearby
stream, the salt water originated from Admiralty
Bay surface waters near the shore. The guano solu�
tion was prepared as follows: dry penguin guano
was collected on the beach of Admiralty Bay and
then drawn in a jar filled with fresh water in the
proportion of approximately 100 g dry guano per
1 liter of water. Irrigation of all types was per�
formed by pouring the solutions under D. antarcti�
ca clumps. Irrigation was performed on a daily
basis at noon every day from 12/14/2005 until
01/12/2006.
ISSN 0564–3783. Цитология и генетика. 2011. № 344
I.Yu. Parnikoza, P. Loro, N.Yu. Miryuta et al.
Fig. 1. Localization of study plot in the environs of Arctowski Station, King George Island, South Shetland Islands, maritime
Antarctic
Biometric analysis. At the beginning and in the
end of the experiment, the following biometric
parameters were measured at all plots: morphologi�
cal features of generative plants, namely the height
of the generative shoot (the distance between the
ground surface and the top of the most distal spikelet
in the inflorescence), the length of the largest leaf
(from the ramification point to the leaf apex), the
number of flowers in the inflorescence and the leaf
condition (dry or green). For each plot, 20 indi�
vidual plants were measured, the mean values cal�
culated (SD allowed for) and compared.
Chlorophyll analysis. The influence of irrigation
type on chlorophyll content in green leaves of D.
antarctica was also addressed. This trait was studied
on samples collected from all experimental plots
on the 30th day of the experiment. For this purpose,
we used the most common acetone method after
McKinney (1941) modified by Starnes & Hadley
(1965). The selected plant samples were grinded in
a mortar with adding 85 % acetone solution. After
homogenization, the samples were left in the mor�
tar for 2–3 hours in a dark place for chlorophyll
extraction from plant tissues. The resulting chloro�
phyll solution was filtered and poured into a gradu�
ated cylinder. The solution was brought up with
acetone to the volume of 25 ml. Chlorophyll light
absorbtion measurements were made using the
spectrophotometer Cintra 20 (GBC Scientific
Equipment, USA). Chlorophyll content in the
leaves was calculated using the samples of
McKinney and Wellburne [22, 23].
Cytological parameters. The following cytogenet�
ic parameters of the leaves, parenchyma, and cells
of D. antarctica from each plot were studied: the
relative content of DNA (for which purpose the
cells were stained after Feulgen) and the nucleus
area. The leaves were fixed for the analysis on the
1st (12/14/2005), 7th (12/20/2005) and 30th (01/12/
2006) day of experiment. For the DNA cytopho�
tometry assay, the lowest third part of a leaf of a vis�
ibly undamaged mature generative plant was taken.
After fixation in 100 % alcohol�acetic acid (3:1
v/v), material was kept in 70 % alcohol. Parenchyma
of the leaves collected for the nucleus area and the
relative DNA content (in follow nuclear DNA con�
tent) assays were then stained according to Feulgen
[24] (Fig. 2).
The green light filter of the microscope optical
system and the red Pal�n one of Asus V 3000 were
used. Four samples from each locality were ana�
lyzed, every sample contained 25 nuclei. The analy�
sis was performed using the digital camera Samsung
CCD SAC�410 PA, videodriver Asus V 3000, and
software packages Corel Draw 7.0, Photo Paint
7.0, and Scion Image (Scion Corporation, USA).
The nucleus area on the images was measured in
pixel units and then converted into SI. RDC was
estimated by comparing the staining intensity of
the nuclei to that of the anaphase nuclei of D.
antarctica rootlet cells in which the quantity of
DNA was estimated to be 4С [21]. The values
obtained for both parameters were broken down
into morphometric classes (Table 1).
Based on the estimated frequency of each class,
distribution curves were plotted for each parameter
over all localities. To compare the curves and to
determine the confidence intervals for correlation
values we applied modificated the Median Test [25].
ІSSN 0564–3783. Цитология и генетика. 2011. № 3 45
The influence of some environmental factors on cytological and biometric parameters
10 mμ
Fig. 2. The parenchyma leaf cells of D. antarctica stained
for nuclear area and DNA content measure according to
Feulgen
Results. The results of the experiment on the
influence of some modeled natural factors on D.
antarctica biometrics are summarized in Table 2.
The generative shoot height at fresh water and
guano plots significantly exceeded that of the con�
trol plants. However, the largest difference was
found at the plot watered with guano solution.
Besides, both guano and the other two experimen�
tal factors induced significant increase of the length
of the largest leaf, with the guano influence being
the most profound. Under guano treatment condi�
tions, the general D. antarctica growth enforcement
was accompanied by appearance of new green
leaves (unlike in plants from other plots, including
control, with yellowish and partially dried leaves).
The number of flowers per inflorescence never dif�
fered significantly from control, though in whole
this trait may vary in different conditions [26].
As to chlorophyll a, its content increased after
fresh water irrigation, but a particularly notable
effect was observed under treatment with the
guano solution. Chlorophyll b content significant�
ly increased only in plants watered with the guano
solution (Table 3).
During the whole experiment and under differ�
ent treatments, we observed cells with the nuclear
area within <10 – �70 μm2, while the relative
nuclear DNA content ranged between <1–10.9 C.
The range of variability, as well as the dominating
class based on these traits in different experimental
conditions, are summarized in Tables 4 and 5.
Experiments on the influence of some natural
factors on the nucleus area and DNA content in
the nuclei of leaf parenchyma cells of D. antarctica
demonstrated that under control conditions statis�
tically significant changes in nucleus area occurred
on the 7th and 30th days of the experiment as com�
pared to the original state (Table 6). Along with
this, significant changes in DNA content in the
nuclei of these cells in control plants have not been
registered.
Salt water and guano treatments differed from
control only in the absence of significant changes
of the nuclear area on the 7th day of the experiment.
Fresh water treatment didn’t induce nuclear area
changes on the 30th day (last day), as well as it did
induce DNA content changes on the 30th day of the
experiment unlike in all other types (in guano
solution case this occurred on the 7th day), which
renders them indistinguishable from control on
30th day.
Discussion. Comparisons between the influ�
ence of some natural factors on D. antarctica bio�
metrics demonstrate that both at fresh water and
guano plots the generative shoot height signifi�
cantly exceeded that of control, probably due to
certain additional watering.
The largest difference from the control was
found at the plot watered with guano solution,
probably due to a combination of two favorable
factors: water as the main one and guano as rein�
forcement. In conditions where mineral complex�
es of the underlying rocks are rich [27], the main
limiting factor seems to be humidity. Meanwhile
none of the analyzed factors was found to induce
significant changes in the number of flowers. This
agrees quite well with data in literature, as several
researchers reported seed yield in this species to
increase under unfavorable conditions, such as
clump densening, decrease water or biogens avail�
ability. In relatively favorable conditions seed
yields decreased [13, 28]. However, during our
experiment, irrigation, and, thus, better condi�
tions, did not produce any decrease in the number
of flowers per inflorescence. This allows specula�
tion that this trait may be highly conservative.
Another explanation could be that the experimen�
tal treatment period might have been insufficient
for significant changes to occur. However, it should
be noted that treatment of each type was per�
formed during the key vegetation period for D.
antarctica (30 days), when mean temperatures
remain above 0 °С. Additionally, during the whole
experimental period a general increase in the growth
rate of hairgrass was noticed as indicated by the
ISSN 0564–3783. Цитология и генетика. 2011. № 346
I.Yu. Parnikoza, P. Loro, N.Yu. Miryuta et al.
Table 1
The values of morphometric classes for nucleus area
and the relative DNA content
Class number Nuclear DNA content,
C
Nucleus area, μm2
1
2
3
4
5
6
7
8
<10
10–19.9
20–29.9
30–39.9
40–49.9
50–59.9
60–69.9
�70
<1
1–2.9
3–4.9
5–6.9
7–8.9
9–10.9
�11
–
appearance of green leaves at the plot with guano
treatment.
Only after our experiment had ended we found
out that Polish researchers conducted a similar
experiment during the season of 1988 near the
Arctowski Station. They planted clumps of similar
size of D. antarctica та Colobanthus quitensis (in
three combinations and three trials) which were
irrigated with a guano solution, urine, and fresh
water. However, no indications on how long the
treatment continued and how regular it was were
present in publications. In 1990, it was revealed
that C. quitensis died out completely, while hair�
grass grew slowly, and its cover had closed in. The
best growth was observed at plots fertilized with a
urea solution, guano�treated plants felt a little
worse, and only a few plants vegetated at fresh
water irrigated plots [12, 13]. The general growth
increase under increased nutrient content condi�
tions is known for other plants as well [29].
The increase of the positive effect of watering
and solved nutrients is corroborated by the elevat�
ed levels of chlorophyll a content under both treat�
ment types, while their complementary action
only in the case with guano solution treatment.
Along with this, it is known that both increase in
available moisture and the effect of organics may,
depending on conditions, increase, decrease, or
leave without changes chlorophyll content
[29–31].
It can be noticed also that the registered differ�
ence in chlorophyll a and b contents in control
possibly explains the yellowish color of the above�
ground parts of hairgrass from the control plot. In
the same time, the rich green color acquired by the
plants at the guano treated plot was accompanied
with a significant increase in chlorophyll b con�
tent. Chlorosis resulting from misbalance in the
ratio between chlorophylls a and b is known from
literature [32]. It has also been pointed out that
increase in chlorophyll b content may take place
due to considerable ramification of a plant and,
thus, decreased availability of light to some parts of
the plant [29], which has not been registered in our
case.
Increase in chlorophyll a and b contents as a
reaction to favorable conditions, specifically
increased availability of water, is probably not
restricted to Antarctic vascular plants; as such
mechanism has also been demonstrated for plants,
for instance for maritime Antarctic mosses [7].
In case of cytological traits, it should be taken
into account that the revealed changes of the
nuclear area in control during the whole experi�
mental period indicate that all four plots experi�
enced equal impact of natural fluctuations of the
available moisture and other factors. Meanwhile
the difference in the effect of all studied factors
from that in control allows considering these fac�
tors as primary causative agents of changes in the
nuclear area and DNA content, as well as of, prob�
ably, cell activity in all three cases. Similarity has
been found between salt water and guano treat�
ment effects which both do not induce nuclear
area changes on the 7th day. This trend in both indi�
cated cases disappears by the end of the experi�
ІSSN 0564–3783. Цитология и генетика. 2011. № 3 47
The influence of some environmental factors on cytological and biometric parameters
Table 2
The influence of some modeled natural factors on biometrics of the generative plants of D. antarctica
Variant
Control
Fresh water
Sea water
Guano solution
2.7 � 0.19/0.7
3.2 � 0.18/3.2
3 � 0.21/0.9
3.8 � 0.18/0.68
0.9 � 0.07/0.1
1.5 � 0.09/1.8
1.2 � 0.08/0.1
2.2 � 0.13/0.3
8.2 � 0.35/2.4
9.0 � 0.64/8.3
8.1 � 0.42/3.6
7.8 � 0.56/6.3
Some dry leafs
Some dry leafs
Some dry leafs
Without dry leafs
Generative shoot
height, cm
Length of biggest
leaf, cm
Number of flowers Leaf condition
Table 3
The influence of the some modeled natural factors on the
content of leaf chlorophyll a and b (mg g–1 DW)
in D. antarctica in situ
Variant
a b a + b
Chlorophyll
Control
Fresh water
Sea water
Guano solu�
tion
1,286 ± 0,149
2,107 ± 0,206
1,259 ± 0,262
3,234 ± 0,178
0,693 ± 0,286
0,685 ± 0,129
0,701 ± 0,321
1,304 ± 0,100
1,979 ± 0,403
2,800 ± 0,322
1,960 ± 0,581
4,539 ± 0,224
ment, which may be connected with fluctuations
in development of physiological reactions in plants
under the treatment conditions.
The statistically significant impacts of guano
treatment on the DNA content registered on the
7th (in guano solution case) and 30th (in fresh water
case) days seem to indicate that fluctuations of
physiological processes (which are detected by this
characteristic) took place during the whole period
or at some stages of the experiment.
Therefore, the influence of such factors like
irrigation with fresh water, salt water or guano
solution directly induces a reaction of physiologi�
cally important traits in individual plants of D.
antarctica, which may cause changes in their bio�
mass, reproductive potential, and cenotic activity,
and consequently potential variability of the
cenoses. Fluctuations of physiologically significant
traits, such as the nuclear area and DNA content
in the leaf parenchyma cells of D. antarctica, under
the influence of the studied factors have been
traced. Understanding the hierarchy of the influ�
ence of these factors, as well as the sensitivity of
the plants of this species to external agents,
requires further investigation.
ISSN 0564–3783. Цитология и генетика. 2011. № 348
I.Yu. Parnikoza, P. Loro, N.Yu. Miryuta et al.
Table 4
The influence of the some modeled natural factors on the general range of variability (a) and the dominant class (b)
and its percentage frequency based on the nuclear area of leaf parenchyma cells in D. antarctica
μm2
Variant а b
1st day
а b
7st day
а b
30st day
Control
Fresh water
Sea water
Guano solution
30–30.9 (63 %)
30–30.9 (58 %)
30–30.9 (52 %)
40–40.9 (42 %)
20–59.9
20–59.9
10–59.9
20–69.9
20–69.9
20–69.9
20–59.9
20–69.9
30–30.9 (49 %)
30–30.9 (46 %)
30–30.9 (69 %)
30–39.9 (42 %)
20 – 79.9
20 – �70
<10 – �70
20 – �70
40–49.9 (36 %)
50–59.9 (37 %)
40–49.9 (32 %)
40–49.9 (27 %)
Variant а b
1st day
а b
7st day
а b
30st day
Control
Fresh water
Sea water
Guano solution
Control
Fresh water
Sea water
Guano solu�
tion
χ2 > 3.84*
χ2 > 3.84
–
–
–
–
–
χ2 > 3.84
χ2 > 3.84
–
χ2 > 3.84
χ2 > 3.84
–
χ2 > 3.84
–
–
3–4.9 (72 %)
3–4.9 (82 %)
3–4.9 (65 %)
3–4.9 (72 %)
1–8.9
1–6.9
3–8.9
1–8.9
1–6.9
1–6.9
1–6.9
1–6.9
3–4.9 (85 %)
3–4.9 (80 %)
3–4.9 (80 %)
3–4.9 (75 %)
1–12.9
1–8.9
1–10.9
<1–10.9
5–6.9 (49 %)
3–4.9 (72 %)
3–4.9 (53 %)
5–6.9 (55 %)
Table 5
The influence of the some modeled natural factors on the general variability range (a) and the dominant class (b)
based on the relative nuclear DNA content in leaf parenchyma cells of D. antarctica
Table 6
The influence of the some modeled natural factors on
changes in the nuclear area and DNA content of leaf
parenchyma cells of D. antarctica in situ
Variant
Nuclear
area
DNA
content
7st day
Nuclear
area
DNA
content
30st day
* Events of significant change of a parameter on the day 7 and
day 30 from the start of the experiment under different treat�
ment types calculated by median test with given probabili�
ty of χ2 > 3.84 have been presented.
C
Authors thank the Ukrainian Antarctic Scientific
Center and the Department of Antarctic Biology of
Polish Academy of Sciences, particularly Prof. S. Ra�
kusa�Suszczewski, and participants of 30�th Polish
Antarctic expedition K. Lypszyc and A. Rjabokon
for their help in implementation of this study. We
thank also A. Rogok and E. Denis for their assistance
in preparing the article. This work has been realized
in frame of research project «The effects of environ�
mental changes on distribution, abundance and
diversity of biota in terrestrial ecosystems of the
Maritime Antarctic» in scientific cooperation of
NASU and PAS and supported by the Young Scientists
grant of President of Ukraine (GP/F11/0048)
И.Ю. Парникоза, П. Лоро, Н.Ю. Мирюта,
В.А. Кунах, И.А. Козерецкая
ВЛИЯНИЕ НЕКОТОРЫХ ФАКТОРОВ
ОКРУЖАЮЩЕЙ СРЕДЫ НА ЦИТОЛОГИЧЕСКИЕ
И БИОМЕТРИЧЕСКИЕ ПАРАМЕТРЫ, А ТАКЖЕ
СОДЕРЖАНИЕ ХЛОРОФИЛЛА
У DESCHAMPSIA ANTARCTICA DESV.
ПРИБРЕЖНОЙ АНТАРКТИКИ
В условиях оазиса Поинт Томаса (остров Короля
Георга, Южные Шетлендские острова) было изучено
влияние искусственной обработки в течение месяца
пресной, морской водой и раствором гуано на био�
метрические показатели, содержание хлорофиллов, а
также площадь ядра паренхимных клеток листка и со�
держание ядерной ДНК в них у растений аборигенно�
го вида Прибрежной Антарктики – antarctica. Искус�
ственное влияние факторов окружающей среды выз�
вало увеличение высоты генеративного побега и дли�
ны наибольшего листа, но не влияло на количество
цветков. Обработка раствором гуано увеличивала со�
держание хлорофиллов a и b, а пресной водой – толь�
ко содержание хлорофилла a. Выявлены признаки
флуктуации физиологически значимых параметров:
площади ядра и содержания ДНК клеток паренхимы
листа D. antarctica, для выяснения иерархии которых,
а также степени чувствительности к внешним факто�
рам требуются более детальные исследования.
І.Ю. Парнікоза, П. Лоро, Н.Ю. Мірюта,
В.А. Кунах, І.А. Козерецька
ВПЛИВ ДЕЯКИХ ФАКТОРІВ ДОВКІЛЛЯ НА
ЦИТОЛОГІЧНІ ТА БІОМЕТРИЧНІ ПАРАМЕТРИ,
А ТАКОЖ ВМІСТ ХЛОРОФІЛУ
DESCHAMPSIA ANTARCTICA DESV.
ПРИБЕРЕЖНОЇ АНТАРКТИКИ
В умовах оази Поінт Томаса (острів Короля Георга,
Південні Шетлендські острови) було вивчено вплив
штучної обробки протягом місяця прісною, морською
водою та розчином гуано на біометричні показники,
вміст хлорофілів, а також площу ядра паренхімних
клітин листка та вміст ядерної ДНК в них, у рослин
аборигенного виду Прибережної Антарктики – antarcti�
ca. Штучний вплив факторів довкілля викликав збіль�
шення висоти генеративного пагону та довжини най�
більшого листка, але не впливав на кількість квіток.
Обробка розчином гуано збільшувала вміст хлорофі�
лів a та b, а прісною водою – тільки вміст хлорофілу a.
Виявлено ознаки флуктуації фізіологічно�значущих
параметрів: площі ядра та вмісту ДНК клітин паренхі�
ми листка D. antarctica, з’ясування ієрархії яких, а та�
кож ступеню чутливості до зовнішніх факторів потре�
бує детальнішого дослідження.
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ISSN 0564–3783. Цитология и генетика. 2011. № 350
I.Yu. Parnikoza, P. Loro, N.Yu. Miryuta et al.
|
| id | nasplib_isofts_kiev_ua-123456789-66842 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 0564-3783 |
| language | English |
| last_indexed | 2025-12-07T17:44:25Z |
| publishDate | 2011 |
| publisher | Інститут клітинної біології та генетичної інженерії НАН України |
| record_format | dspace |
| spelling | Parnikoza, I.Yu. Loro, P. Miryuta, N.Yu. Kunakh, V.A. Kozeretska, I.A. 2014-07-23T08:04:54Z 2014-07-23T08:04:54Z 2011 The influence of some environmental factors on cytological and biometric parameters and chlorophyll content of Deschampsia antarctica Desv. in the maritime Antarctic / І.Yu. Parnikoza, P. Loro, N.Yu. Miryuta, V.А. Kunakh, I.А. Kozeretska // Цитология и генетика. — 2011. — Т. 45, № 3. — С. 43-50. — Бібліогр.: 32 назв. — англ. 0564-3783 https://nasplib.isofts.kiev.ua/handle/123456789/66842 292.3+551.8+582.35/.99+575.17 Under the environmental conditions of the Point Thomas Oasis (King George Island, the South Shetland Islands), we studied the influence of month-long artificial treatment with fresh water, salt water, and guano solution on the biometric characteristics, chlorophyll content, as well as the nuclear area of leaf parenchymal cells and nuclear DNA content, in a maritime Antarctic aboriginal plant Deschampsia antarctica. The modeled factors induced an increase in the generative shoot height and the length of the largest leaf, but did not influence the number of flowers. Treatment with guano caused an increase in the chlorophyll a and b contents, while fresh water treatment only led to some increase in chlorophyll a. Fluctuations of physiologically significant traits, such as the nuclear area and DNA content in the leaf parenchyma cells of D. antarctica, have been traced under the influence of the studied factors. Understanding of the hierarchy of influence of these factors as well as and sensitivity of plants of this species to external agents require further investigation. В условиях оазиса Поинт Томаса (остров Короля Георга, Южные Шетлендские острова) было изучено влияние искусственной обработки в течение месяца пресной, морской водой и раствором гуано на биометрические показатели, содержание хлорофиллов, а также площадь ядра паренхимных клеток листка и содержание ядерной ДНК в них у растений аборигенного вида Прибрежной Антарктики – antarctica. Искусственное влияние факторов окружающей среды вызвало увеличение высоты генеративного побега и длины наибольшего листа, но не влияло на количество цветков. Обработка раствором гуано увеличивала содержание хлорофиллов a и b, а пресной водой – только содержание хлорофилла a. Выявлены признаки флуктуации физиологически значимых параметров: площади ядра и содержания ДНК клеток паренхимы листа D. antarctica, для выяснения иерархии которых, а также степени чувствительности к внешним факторам требуются более детальные исследования. В умовах оази Поінт Томаса (острів Короля Георга, Південні Шетлендські острови) було вивчено вплив штучної обробки протягом місяця прісною, морською водою та розчином гуано на біометричні показники, вміст хлорофілів, а також площу ядра паренхімних клітин листка та вміст ядерної ДНК в них, у рослин аборигенного виду Прибережної Антарктики – antarctica. Штучний вплив факторів довкілля викликав збільшення висоти генеративного пагону та довжини найбільшого листка, але не впливав на кількість квіток. Обробка розчином гуано збільшувала вміст хлорофілів a та b, а прісною водою – тільки вміст хлорофілу a. Виявлено ознаки флуктуації фізіологічно-значущих параметрів: площі ядра та вмісту ДНК клітин паренхіми листка D. antarctica, з’ясування ієрархії яких, а також ступеню чутливості до зовнішніх факторів потребує детальнішого дослідження. Authors thank the Ukrainian Antarctic Scientific Center and the Department of Antarctic Biology of Polish Academy of Sciences, particularly Prof. S. Rakusa-Suszczewski, and participants of 30-th Polish Antarctic expedition K. Lypszyc and A. Rjabokon for their help in implementation of this study. We thank also A. Rogok and E. Denis for their assistance in preparing the article. This work has been realized in frame of research project «The effects of environmental changes on distribution, abundance and diversity of biota in terrestrial ecosystems of the Maritime Antarctic» in scientific cooperation of NASU and PAS and supported by the Young Scientists grant of President of Ukraine (GP/F11/0048). en Інститут клітинної біології та генетичної інженерії НАН України Цитология и генетика Оригинальные работы The influence of some environmental factors on cytological and biometric parameters and chlorophyll content of Deschampsia antarctica Desv. in the maritime Antarctic Вплив деяких факторів довкілля на цитологічні та біометричні параметри, а також вміст хлорофілу у Deschampsia antarctica Desv. прибережної Антарктики Влияние некоторых факторов окружающей среды на цитологические и биометрические параметры, а также содержание хлорофилла у Deschampsia antarctica desv. прибрежной Антарктики Article published earlier |
| spellingShingle | The influence of some environmental factors on cytological and biometric parameters and chlorophyll content of Deschampsia antarctica Desv. in the maritime Antarctic Parnikoza, I.Yu. Loro, P. Miryuta, N.Yu. Kunakh, V.A. Kozeretska, I.A. Оригинальные работы |
| title | The influence of some environmental factors on cytological and biometric parameters and chlorophyll content of Deschampsia antarctica Desv. in the maritime Antarctic |
| title_alt | Вплив деяких факторів довкілля на цитологічні та біометричні параметри, а також вміст хлорофілу у Deschampsia antarctica Desv. прибережної Антарктики Влияние некоторых факторов окружающей среды на цитологические и биометрические параметры, а также содержание хлорофилла у Deschampsia antarctica desv. прибрежной Антарктики |
| title_full | The influence of some environmental factors on cytological and biometric parameters and chlorophyll content of Deschampsia antarctica Desv. in the maritime Antarctic |
| title_fullStr | The influence of some environmental factors on cytological and biometric parameters and chlorophyll content of Deschampsia antarctica Desv. in the maritime Antarctic |
| title_full_unstemmed | The influence of some environmental factors on cytological and biometric parameters and chlorophyll content of Deschampsia antarctica Desv. in the maritime Antarctic |
| title_short | The influence of some environmental factors on cytological and biometric parameters and chlorophyll content of Deschampsia antarctica Desv. in the maritime Antarctic |
| title_sort | influence of some environmental factors on cytological and biometric parameters and chlorophyll content of deschampsia antarctica desv. in the maritime antarctic |
| topic | Оригинальные работы |
| topic_facet | Оригинальные работы |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/66842 |
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