Experimental study of the plasticostimulating effect of drugs based on the synthetic polymers when topically applied
Експериментально на білих щурах встановлено, що препарати на основі синтетичних полімерів: органічного кремнію (силікін), поліамідного гелю (акваліфт), диметиламінометанолу (ДМАЕ) при внутрішньослизовому введенні у дозі 0,1 мл не викликають місцевих негативних реакцій у тварин. При морфологічному...
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Кримський науковий центр НАН України і МОН України
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| Cite this: | Experimental study of the plasticostimulating effect of drugs based on the synthetic polymers when topically applied / V.M. Zubachyk, M.O. Iskiv, I.V. Gan, A.M. Yashchenko // Таврический медико-биологический вестник. — 2013. — Т. 16, № 1, ч. 2 (61). — С. 58-60. — Бібліогр.: 5 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1859739835253653504 |
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| author | Zubachyk, V.M. Iskiv, M.O. Gan, I.V. Yashchenko, A.M. |
| author_facet | Zubachyk, V.M. Iskiv, M.O. Gan, I.V. Yashchenko, A.M. |
| citation_txt | Experimental study of the plasticostimulating effect of drugs based on the synthetic polymers when topically applied / V.M. Zubachyk, M.O. Iskiv, I.V. Gan, A.M. Yashchenko // Таврический медико-биологический вестник. — 2013. — Т. 16, № 1, ч. 2 (61). — С. 58-60. — Бібліогр.: 5 назв. — англ. |
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| description | Експериментально на білих щурах встановлено, що препарати на основі синтетичних полімерів:
органічного кремнію (силікін), поліамідного гелю (акваліфт), диметиламінометанолу (ДМАЕ) при
внутрішньослизовому введенні у дозі 0,1 мл не викликають місцевих негативних реакцій у тварин. При
морфологічному дослідженні виявлено рівномірне включення пластикостимуляторів у міжклітинний
матрикс, їх здатність збільшувати кількість і активність фібробластів, сприяти ангіогенезу та трофіці тканин.
Це зумовило формування сполучної тканини і спричинило потовщення субепітеліального шару слизової
оболонки порожнини рота.
Экспериментально на белых крысах показано, что препараты, содержащие синтетические
полимеры: органический кремний (силикин), полиамидный гель (аквалифт), диметиламинометанол
(ДМАЕ) при внутрислизистом введении 0,1 мл не вызывают местных негативных реакций у животных. При
морфологическом исследовании выявлено равномерное включение пластикостимуляторов в межклеточный
матрикс, их свойство увеличивать количество и активность фибробластов, содействовать ангиогенезу
и трофике тканей. Это обусловило формирование соединительной ткани и содействовало утолщению
субэпителиального слоя слизистой оболочки полости рта.
|
| first_indexed | 2025-12-01T16:13:21Z |
| format | Article |
| fulltext |
ТАвРИЧЕСкИЙ мЕДИкО-бИОЛОГИЧЕСкИЙ вЕСТНИк2013, том 16, №1, ч.2 (61)
UDC 616.311:615.462] –092.8
© Group of the authors, 2013
EXPERIMENTAL STUDY OF THE PLASTICOSTIMULATING EFFECT
OF DRUGS BASED ON THE SYNTHETIC POLYMERS WHEN
TOPICALLY APPLIED
v. M. Zubachyk, M. o. iskiv, i. v. gan, a. M. yashchenko*
Department of Therapeutic Dentistry (Head.– D. M. Sci, Prof. V. M. Zubachyk); *Department of Histology, Cell biology and
Embryology (Head – D. M. Sci., Prof. O. D. Lutsyk), Danylo Halytskyi National Medical University in Lviv. 79010, Ukraine, Lviv,
Pekarska str.,69 B. Е-mail: zubachyk@meduniv.lviv.ua
ЕКСпЕРИмЕНТАЛьНЕ ДОСЛіДЖЕННЯ пЛАСТИКОСТИмУЛЮВАЛьНОГО ЕФЕКТУ пРЕпАРАТіВ НА
ОСНОВі СИНТЕТИчНИх пОЛімЕРіВ пРИ міСЦЕВОмУ ЗАСТОСУВАННі
В. м. Зубачик, м. О. іськів, і. В. Ган, А. м. Ященко
РЕЗюМЕ
Експериментально на білих щурах встановлено, що препарати на основі синтетичних полімерів:
органічного кремнію (силікін), поліамідного гелю (акваліфт), диметиламінометанолу (ДМАЕ) при
внутрішньослизовому введенні у дозі 0,1 мл не викликають місцевих негативних реакцій у тварин. При
морфологічному дослідженні виявлено рівномірне включення пластикостимуляторів у міжклітинний
матрикс, їх здатність збільшувати кількість і активність фібробластів, сприяти ангіогенезу та трофіці тканин.
Це зумовило формування сполучної тканини і спричинило потовщення субепітеліального шару слизової
оболонки порожнини рота.
эКСпЕРИмЕНТАЛьНОЕ ИССЛЕДОВАНИЕ пЛАСТИКОСТИмУЛИРУЮЩЕГО эФФЕКТА пРЕпАРАТОВ
НА ОСНОВАНИИ СИНТЕТИчЕСКИх пОЛИмЕРОВ пРИ мЕСТНОм пРИмЕНЕНИИ
В. м. Зубачик, м. О. Иськив, И. В. Ган, А. м. Ященко
РЕЗюМЕ
Экспериментально на белых крысах показано, что препараты, содержащие синтетические
полимеры: органический кремний (силикин), полиамидный гель (аквалифт), диметиламинометанол
(ДМАЕ) при внутрислизистом введении 0,1 мл не вызывают местных негативных реакций у животных. При
морфологическом исследовании выявлено равномерное включение пластикостимуляторов в межклеточный
матрикс, их свойство увеличивать количество и активность фибробластов, содействовать ангиогенезу
и трофике тканей. Это обусловило формирование соединительной ткани и содействовало утолщению
субэпителиального слоя слизистой оболочки полости рта.
key words: sylikin, aqualift, dMae, oral mucosa, histomorphology.
Aesthetic aspects are an integral part of the compre-
hensive concept of treating dental patients. It concerns
not only the harmony in shape, size and color of teeth,
but also the outline of lips and gums. Less attention is
paid to the latter one, but the shape, contour, thickness,
features of the gingival papillae cause the perception of
the gums beauty [1, 3]. Therefore, the scientific rationale
as to both the choice of medicine and best methods of
their application for the removal of the aesthetic soft
tissue defects through minimally invasive contouring
were the subject of our experimental study.
The aim of the work was to analyze the histomor-
phological changes in the rats’ mucous membrane after
local application of the drug based on the compounds
of the synthetic origin.
MATERIALS AND METHODS
The examination was carried out as an experiment
on 30 white rats aged 9–10 weeks, weighing 210–230 gr.
Interventions were performed under the general anaes-
thesia (0,5 ml of 4 % solution of thiopental sodium into
the peritoneum). Animals were injected intramucously
with 0,1 ml of the studied medicine into the inside of the
cheek in the area near the molars close to the transition
convolution of the alveolar process of the upper maxilla
with the depth of the injection up to 2mm.
All animals were divided into 6 experimental groups
of 5 animals each: the first group – intact animals served
as a control, the second group – animals which were
injected with placebo (saline); and the third group – ani-
mals which were injected intramucously with the drug
based on the solution of organic silicon „Sylikin 1 %”
(„ID-Farma”, Spain); the fourth group – animals injected
with hydrogel „Aqualift” (JSC „National Center for
Medical Technology”, Ukraine); the fifth group – animal
injected with 2-demethylaminoethanol 4-ethanolatce-
toaminobenzoat 6 % – a synthetic medicine „DMAE”
(OOO „Laboratory of Tuscany”, Russia).
The condition of the mucous membrane of the buc-
cal cavity in each rat was determined due to the results
of the visually-instrumental and histological studies. On
the 30th day of the experiment, euthanasia of the rats
was performed under the thiopental anaesthesia (20 mg/
kg), in the injection site, areas of the mucous membrane
together with the adjacent „healthy” areas of the tissue
were carved. Samples of the tissue were fixed in the 10 %
58
ОРИГИНАЛЬНЫЕ СТАТЬИ
neutral formalin and embedded in paraffin. Paraffin sec-
tions with the thickness up to 7,5 mkm were produced
on a rotary microtomes, stained with hematoxylin and
eosin. Viewing and photography of the preparations
were carried out using video system Nicon Eclips 200.
THE RESULTS AND DISCUSSION
Macroscopically, oral mucous membrane of the first
control group is of pale pink colour, moist, with gleam
and expressed tension without folds and visible damage.
The results of the histological examination confirmed
that the mucous membrane of the cheeks of the intact
animals is covered with multilayered flat epithelium,
in which a basal, spinous, granular and thin, oxyphilic-
colored, horny layer is differentiated. Net plate of the
mucous membrane is formed by loose connective tissue
with a large number of cellular elements, among which
fibroblasts dominate. It forms high papillae and smoothly
develops into the submucosa containing bundles of col-
lagen fibers, a large number of cellular elements and
muscle fibers. Net plate and submucous base are well
vascularized, in maxillary cheek area has finite secretory
departments of the protein- mucous glands.
The animals of the second group after the single lo-
cal injection of 0,1 ml of saline within a prolonged long
period of observation, visible morphological changes in
the local area of the drug use were not found. Mucous and
submucous base had distinctive layered structure which
was typical for the healthy animals of the control group.
Thus, the intramucous placebo injection does not affect
the morphological and functional condition of animals’
mucosa. Therefore the results of the examination of the
injectional usage of different, as to drugs may be com-
pared and analytically considered.
In the third group of rats on the 30th day after the
injection of a 1 %- sylikin into the buccal mucosa neither
gel or their encapsulation or formation of cavities were
not diagnosed. The obtained results testify bioinert-
ness of the drug. The results of the histological studies
showed, that the mucous membrane of the cheek has a
typical structure similar to that of the previous animal
group after the injection of the organic silicon. It is
formed by the epithelial plate, represented by a stratified
epithelium with signs of keratinization. In the epithelial
plate, a basal layer is clearly differentiated (1 row of
highly prismatic cells), spinous layer is formed by 10
rows polygonal-shape cells, among which are ones with
signs of mitosis. Basal and lower rows of the spinous
layer form the germinal (sprout) zone of the laminated
epithelium. The granular layer – two rows of cells, in the
cytoplasm of which there are basophilic colored granules
of keratohyalin. In the granular layer oxyphilic colored
thin stratum corneum lies. Net plate – loose connective
tissue that contains a large number of cellular elements.
Submucous base – the connective tissue that is character-
ized by a large number of thick bundles of collagen fibers,
the muscle fibers are in the distal part, in the submucous
of the maxillary part of the cheek protein-and-mucous
salivary glands are differentiated. Thus, the drug sylikin
after a single injection for a long time does not cause
significant changes in the oral mucosa of the animal.
Taking into consideration the fact, that the organic sili-
con has a clearly marked defibrous effect, promotes the
strength of collagen and elastin [2]; it proves to be use
ful as a medicine not for monotherapy but in combina-
tion with other drugs.
In the fourth group of animals, after the adminis-
tration of the hydrophilic polyamide gel Aqualift, no
clinical complications were found within the experi-
ment. The drug does not move from the input zone, is
not different in texture from soft tissues, is biocompat-
ible, not allergic, does not cause an irritant effect and
inflammatory reactions. It is histologically defined that,
mucous membrane is covered with flat epithelium with
keratinization and normal differentiation of the lay-
ers. Net plate does not almost form papillae. In some
areas there are preserved papillary structures against
the narrow subepithelial layer, areas with lower papil-
lary structures and a doublenlarged subepithelial layer
due to proliferation of the epithelium. A great number
of vessels, which are concentrated around the cellular
elements of connective tissue and intensely painted
oxyphilic collagen fibers can be determined. Thus, the
drug Aqualift based on the synthetic polymers creates
conditions under which the synthesis component of
extracellular matrix improves. Branching of the vessels
of the microvasculature that occurs under the influence
of the drug, improves microcirculation, which enhances
the synthetic and metabolic processes and have a positive
impact on the tissue regeneration.
In the fifth group the drug DMAE is evenly dis-
tributed within the mucosa, is not rejected by the body
after its administration. The bioinert allows administer
it repeatedly into the one anatomic site. During the
experiment mucous was not visually altered. After the
DMAE application the mucous membrane is covered
with stratified flat epithelium with keratinization and
differentiation of normal layers. Net plate does not form
papillae. Subepithelial layer is greatly thickened (its
thickness is about twice bigger than the epithelial layer).
In the submucous base between the bundles of collagen
fibers, the number of primary amorphous substances and
accumulation of adipocytes increases. A large number
of vessels and oxyphilic collagen fibers is determined,
blood vessels are dilated, filled with blood corpusles,
among which red blood cells predominate. Taking into
consideration the mechanisms of action, DMAE may
cause subclinical vasodilatation, transient swelling and
accumulation of water in the hydrophilic structures of
the subepithelial layer that is required to supply and
maintain the function of the mucous membrane [4]. Mi-
nor accumulations of cellular elements around vessels,
infiltration of the net plate and submucosal membrane
with polymorphonuclear leukocytes (immune stimula-
59
ТАвРИЧЕСкИЙ мЕДИкО-бИОЛОГИЧЕСкИЙ вЕСТНИк2013, том 16, №1, ч.2 (61)
tion processes) were observed. Thus, the DMAE drug
affects not only the extracellular matrix, enhancing the
processes of cell proliferation, but also as a stimulant
acetylcholine receptor cells and endothelial sprout zone,
causing activation of growth and differentiation of the
cells. It is involved in the regulation of basic cellular
functions [5], thereby changing the structure of intercel-
lular space, the elasticity of the soft tissues increases and
plastico stimulating effect is realized.
CONCLUSIONS
1. Macroscopically, on the 30th day of the experi-
ment carried on rats, in the area of localization of drugs
gel conglomerates or solution, their encapsulation or
formation of cavities were not diagnosed, grafting
materials are uniformly distributed evenly in the area
of administration and didn’t go beyond it. No signs of
tissue aggression were not observed, drugs are bioin-
ert which allows repeatedly typing them into the one
anatomic site.
2. Microscopically, the formation of the connec-
tive- tissue capsule around the injected gel drug or the
microcapsulation of its fragments were not discovered
in the fragments of the mucous membrane of the cheek.
Interstitial distribution and inclusion of means of remod-
eling into the extracellular matrix was observed, which
caused the increased number and activity of fibroblasts,
formation of bundles of collagen and other connective
tissue components, and moreover improved angiogen-
esis and trophic of local tissues. As a result a significant
thickening of the subepithelial layer and expressed
plastic stimulation, occurred.
REFERENCES
1. Bekker U. et al. Minimal-invasive treatment of
papillas defects in esthetic zone. / U. Bekker, I. Gabitov,
M. Stepanov [et al.] // Dentistry.– 2011.– № 1 (151).– P.
14–19.
2. Porfenova I. A. Microelements in correction
programs of esthetic face and body problems / I. A. Por-
fenova // Mesotherapy.– 2010.– № 11.– P. 38–46.
3. Rafiy C. Treatment possibilities in esthetic zone
/C. Rafiy // New in Dentistry.– 2011.– № 2.–P. 6–27.
4. Smus V. V. Scientific investigations of DMAE in
Cosmetology / V. V. Smus // Mesotherapy.– 2008.– Issue.
6–7, № 3–4.– P. 14–18.
5. Uhoda I. [et al.] Split face study on the cutane-
ous tensile effect of 2-dimethylaminoethanol (deanol)
gel /I. Uhoda, N. Faska, C. Robert [et al.] // Skin. Res.
Technol.– 2002.– Vol. 8, № 3.– Р. 164–167.
60
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| id | nasplib_isofts_kiev_ua-123456789-75432 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 2070-8092 |
| language | English |
| last_indexed | 2025-12-01T16:13:21Z |
| publishDate | 2013 |
| publisher | Кримський науковий центр НАН України і МОН України |
| record_format | dspace |
| spelling | Zubachyk, V.M. Iskiv, M.O. Gan, I.V. Yashchenko, A.M. 2015-01-30T08:09:58Z 2015-01-30T08:09:58Z 2013 Experimental study of the plasticostimulating effect of drugs based on the synthetic polymers when topically applied / V.M. Zubachyk, M.O. Iskiv, I.V. Gan, A.M. Yashchenko // Таврический медико-биологический вестник. — 2013. — Т. 16, № 1, ч. 2 (61). — С. 58-60. — Бібліогр.: 5 назв. — англ. 2070-8092 https://nasplib.isofts.kiev.ua/handle/123456789/75432 616.311:615.462]-092.8 Експериментально на білих щурах встановлено, що препарати на основі синтетичних полімерів: органічного кремнію (силікін), поліамідного гелю (акваліфт), диметиламінометанолу (ДМАЕ) при внутрішньослизовому введенні у дозі 0,1 мл не викликають місцевих негативних реакцій у тварин. При морфологічному дослідженні виявлено рівномірне включення пластикостимуляторів у міжклітинний матрикс, їх здатність збільшувати кількість і активність фібробластів, сприяти ангіогенезу та трофіці тканин. Це зумовило формування сполучної тканини і спричинило потовщення субепітеліального шару слизової оболонки порожнини рота. Экспериментально на белых крысах показано, что препараты, содержащие синтетические полимеры: органический кремний (силикин), полиамидный гель (аквалифт), диметиламинометанол (ДМАЕ) при внутрислизистом введении 0,1 мл не вызывают местных негативных реакций у животных. При морфологическом исследовании выявлено равномерное включение пластикостимуляторов в межклеточный матрикс, их свойство увеличивать количество и активность фибробластов, содействовать ангиогенезу и трофике тканей. Это обусловило формирование соединительной ткани и содействовало утолщению субэпителиального слоя слизистой оболочки полости рта. en Кримський науковий центр НАН України і МОН України Таврический медико-биологический вестник Оригинальные статьи Experimental study of the plasticostimulating effect of drugs based on the synthetic polymers when topically applied Експериментальне дослідження пластикостимулювального ефекту препаратів на основі синтетичних полімерів при місцевому застосуванні Экспериментальное исследование пластикостимулирующего эффекта препаратов на основании синтетических полимеров при местном применении Article published earlier |
| spellingShingle | Experimental study of the plasticostimulating effect of drugs based on the synthetic polymers when topically applied Zubachyk, V.M. Iskiv, M.O. Gan, I.V. Yashchenko, A.M. Оригинальные статьи |
| title | Experimental study of the plasticostimulating effect of drugs based on the synthetic polymers when topically applied |
| title_alt | Експериментальне дослідження пластикостимулювального ефекту препаратів на основі синтетичних полімерів при місцевому застосуванні Экспериментальное исследование пластикостимулирующего эффекта препаратов на основании синтетических полимеров при местном применении |
| title_full | Experimental study of the plasticostimulating effect of drugs based on the synthetic polymers when topically applied |
| title_fullStr | Experimental study of the plasticostimulating effect of drugs based on the synthetic polymers when topically applied |
| title_full_unstemmed | Experimental study of the plasticostimulating effect of drugs based on the synthetic polymers when topically applied |
| title_short | Experimental study of the plasticostimulating effect of drugs based on the synthetic polymers when topically applied |
| title_sort | experimental study of the plasticostimulating effect of drugs based on the synthetic polymers when topically applied |
| topic | Оригинальные статьи |
| topic_facet | Оригинальные статьи |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/75432 |
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