Relativistic electron influence on sanitary-model microorganisms and antibiotics in model samples
A series of the investigations of the electron beam influence on sanitary-model test cultures and antibiotics in
 model solutions has been carried out. For each of the test objects, the authors have found the boundary doses of the
 absorbed radiation. The higher doses cause the sharp...
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| Опубліковано в: : | Вопросы атомной науки и техники |
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| Дата: | 2004 |
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Національний науковий центр «Харківський фізико-технічний інститут» НАН України
2004
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| Цитувати: | Relativistic electron influence on sanitary-model microorganisms and antibiotics in model samples / V.S. Antipov, E.M. Babych, I.V. Berezhna, Yu.L. Voliansky, O.Yu. Karpenko*, O.F. Kovpik, E.O. Kornilov, V.A. Kiselev, A.F. Linnik, Yu.I. Sergienkо, N.I. Sklar, V.V. Uskov, T.V. Hirna // Вопросы атомной науки и техники. — 2004. — № 2. — С. 189-191. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1860080869729894400 |
|---|---|
| author | Antipov, V.S. Babych, E.M. Berezhna, I.V. Voliansky, Yu.L. Karpenko, O.Yu. Kovpik, O.F. Kornilov, E.O. Kiselev, V.A. Linnik, A.F. Sergienko, Yu.I. Sklar, N.I. Uskov, V.V. Hirna, T.V. |
| author_facet | Antipov, V.S. Babych, E.M. Berezhna, I.V. Voliansky, Yu.L. Karpenko, O.Yu. Kovpik, O.F. Kornilov, E.O. Kiselev, V.A. Linnik, A.F. Sergienko, Yu.I. Sklar, N.I. Uskov, V.V. Hirna, T.V. |
| citation_txt | Relativistic electron influence on sanitary-model microorganisms and antibiotics in model samples / V.S. Antipov, E.M. Babych, I.V. Berezhna, Yu.L. Voliansky, O.Yu. Karpenko*, O.F. Kovpik, E.O. Kornilov, V.A. Kiselev, A.F. Linnik, Yu.I. Sergienkо, N.I. Sklar, V.V. Uskov, T.V. Hirna // Вопросы атомной науки и техники. — 2004. — № 2. — С. 189-191. — англ. |
| collection | DSpace DC |
| container_title | Вопросы атомной науки и техники |
| description | A series of the investigations of the electron beam influence on sanitary-model test cultures and antibiotics in
model solutions has been carried out. For each of the test objects, the authors have found the boundary doses of the
absorbed radiation. The higher doses cause the sharp increase in the bactericidal influence, which becomes complete. The sanitary-bactericidal indices of the water samples remain stable during 6 days. The samples of antibiotics
in various concentrations (from 100 UA) have been irradiated. It is proved that the substratum processing by the
beam (in the regimes 30 kGy) causes diminution and complete neutralization of the antibacterial activity in all
probes of the samples.
Виконано цикл досліджень по впливу електронного пучка на санітарно-показові тест-культури та
антибіотики в модельних розчинах.. Для кожного тест-об’єкта визначено граничні дози поглинутого
випромінювання, вище яких бактерицидна дія різко посилюється і стає повною. При цьому забезпечується
стабільність санітарно-бактеріологічних показників зразків води протягом 6 діб. Показано, що оброблення
електронним пучком модельних зразків антибіотиків, взятих в концентраціях до 100 ОА, в режимах до 30
кГр приводило до зменшення та повної нейтралізації антибактеріальної активності у всіх пробах зразків.
Выполнен цикл исследований по влиянию электронного пучка на санитарно-показательные тест-культуры и антибиотики в модельных растворах. Для каждого тест-объекта установлены предельные дозы поглощенного облучения, выше которых бактерицидное действие резко усиливается и становится полным. При
этом обеспечивается стабильность санитарно-бактериологических показателей образцов воды на протяжении 6 дней. Показано, что обработка электронным пучком модельных образцов антибиотиков, взятых в концентрациях до 100 ОА, в режимах до 30 кГр приводило к уменьшению и полной нейтрализации антибактериальной активности во всех пробах образцов.
|
| first_indexed | 2025-12-07T17:16:50Z |
| format | Article |
| fulltext |
RELATIVISTIC ELECTRON INFLUENCE ON SANITARY-MODEL
MICROORGANISMS AND ANTIBIOTICS IN MODEL SAMPLES
V.S. Antipov, E.M.Babych*, I.V.Berezhna, Yu.L.Voliansky*, O.Yu. Karpenko*, O.F.Kovpik,
E.O.Kornilov, V.A.Kiselev, A.F.Linnik, Yu.I.Sergienko*, N.I.Sklar*, V.V.Uskov, T.V. Hirna*
NSC Kharkov`s Institute of Physics & Technology, Kharkiv, Ukraine
*Mechnikov Institute of Microbiology and Immunology, Kharkiv, Ukraine
A series of the investigations of the electron beam influence on sanitary-model test cultures and antibiotics in
model solutions has been carried out. For each of the test objects, the authors have found the boundary doses of the
absorbed radiation. The higher doses cause the sharp increase in the bactericidal influence, which becomes com-
plete. The sanitary-bactericidal indices of the water samples remain stable during 6 days. The samples of antibiotics
in various concentrations (from 100 UA) have been irradiated. It is proved that the substratum processing by the
beam (in the regimes 30 kGy) causes diminution and complete neutralization of the antibacterial activity in all
probes of the samples.
PACS: 87.23.-n
1. INTRODUCTION
Lately a new prospective approach to the problem of
the water disinfection takes shape. The approach in-
cludes the application of physical methods (processing
with γ -irradiation, electron beams, ultraviolet and ultra-
sound). The use of electron beams is especially prospec-
tive in the branch of disinfection and purification of
waste- waters. Processing of industrial and domestic ef-
fluents with electron beams can provide not only annihi-
lation of pathogenic bacteria but also purification of ef-
fluents from biological and synthetic admixtures. The
advantage of this method of the water purification is
that that its effectiveness is independent of the water
clarity - i.e., the efficiency of beam energy expenditure
makes 100%.
2. TECHNICAL APPROACH
Microbiological objects have been chosen among
sanitary-model microflora of taxonomic groups - such
as the cultures E.coli, Klebsiella pneumoniae, Enter-
obacter cloacae, Citobacter freundii, Serratia
marcescens as well as the plasma-coagulating staphylo-
coccus S.aureus and enterococcus E.faecalis.
Model samples of the test cultures had been pre-
pared and sowed. Their biological characteristics were
studied before and after processing of the model solu-
tions with physical and chemical agents in accordance
with the methodical guidelines.
The beam influence on antibiotics (penicillin, strep-
tomycin and tetracycline) is investigated by the express-
method. In this method, one applies antimicrobial medi-
cations, able to suppress dehydrogenation activity of test
cultures in a rare nutrient medium.
The processes of water disinfection and purification
have been investigated with a laboratory installation,
elaborated in the NSC KIPT. Its principal functional
units are the following: a linear resonance accelerator of
electrons with a system measuring the beam parameters;
a device for the beam extraction into the atmosphere;
contact reservoirs for the water sample processing by ir-
radiation and a measurer of the absorbed irradiation
dose.
For the water sample irradiation with various por-
tions of relativistic electrons, a linear resonance acceler-
ator of electrons has been used. The parameters of the
beam, generated in the accelerator, are the following:
the electron energy is 3...4 MeV; the electron current is
0.5 A; the current pulse duration is 2 sµ . If the electron
energy is 4 MeV, the track length of electrons in water
amounts to 2.5 cm.
In the experiments, the mode of the accelerator opera-
tion is chosen so that to provide a broad distribution of
the electrons at the accelerator output. The electron ener-
gy losses in the atmosphere diminish, not exceeding
100...150 keV at the distance up to 30 cm from the accel-
erator output window. The irradiation dose absorbed by
the sample is measured with the help of dacryl sensors.
Water samples have been irradiated in test tubes of
the volume (2...3) ml and specially designed 500-ml
volume pans of stainless steel (the height is 50 cm; the
width is 5 cm; the depth is 2 cm). For the pan pressur-
ization, a 50- mµ thick mayoral film is used, which
minimizes the beam energy losses. For irradiation of
samples of large volumes (in the pans), we have
scanned the beam with an electromagnet, placed at the
accelerator output after the extraction window.
3. THE EXPERIMENTAL RESULTS
The study of the beam influence has indicated that,
among the test cultures examined, the following repre-
sentatives of enterobacteria have to be considered as the
most vulnerable to the effect of the given physical
agent: E.coli, E. cloacae, C. Freundii and S. marcescens.
Low-intensive irradiation of model samples of the
given test-cultures (0.8...3.0) kGy has caused the total
annihilation of the bacteria in 54 of the 270 tests (19.6
± 2.4)% during 6 days. In 129 tests (47.0 ± 2.4)%, we
have observed a partial delay in the microbe multiplica-
tion, an amount of the viable cells being substantially
diminished. In other cases (33.4%), the colony-forming
subpopulations have survived in the initial concentra-
tion. These date indicate that, under the given regimes
of irradiation, there takes place the microbial suspension
disinfection mainly due to getting of the biological ob-
jects into the temporally inactive state. The beam bacte-
riostatic aftereffect is characterized by a steep diminu-
tion in the number of the colony-forming units of enter-
obacteria (103...107 times as small). Such delay in the
___________________________________________________________
PROBLEMS OF ATOMIC SIENCE AND TECHNOLOGY. 2004. № 2.
Series: Nuclear Physics Investigations (43), p.189-191.
189
test-culture development has been observed mainly dur-
ing the first hours after the experiment ((62.8 ± 4.3)%).
At the third and sixth days, the number of the model
samples with this specificity has diminished ((2...7)
times as little).
The total annihilation of test cultures of non-capsular
enterobacteria is correlated with the model sample irra-
diation in more intensive regimes - to start from
3.6 kGy.
That is, irradiation of staphylococcus test-cultures
and enterococcus suspensions in the regimes
(0.8...3.0) kGy has not provided for the model sample
total disinfection at the first day. However, at the third
day after the irradiation, the number of the test-objects,
where the beam bacteriostatic effect is detected, has be-
came twice as large. At the sixth day, microbial cells in
all the probes have become sterile. Processing of
staphylococcus cultures and samples of enterococci with
the beam has resulted in the bactericidal effect already
during their irradiation with the doses heightened up to
3.6 kGy.
For instance, in Fig.1 one can see the average in-
dices lg KYO/ml for staphylococci as a function of the
irradiation dose at different days of the observation.
There exist the analogous dependences for the other test
cultures (enterobacteria, enterococci and klebsiella).
0
2
4
6
8
1 0
0 1 2 3 4
lg CFU/ml
D, kGy
321
Fig.1. Average indices lg KYO/ml for staphylococci in
various regimes of the irradiation at the first (1), sec-
ond (2) and third (3) days of the observation
In contrast to the bacteria mentioned above, the total
restoration of the initial concentration of colony-form-
ing units of the subpopulation (109CFU/ml, the CFU
symbol marks the colony-forming units) at the sixth day
after the processing with the beam is typical of Klebsiel-
la model samples but not a partial destruction of viable
cells. An increase in the power load ((3.6...4.0) kGy) on
the given test cultures has caused a stable decrease in
the number of alive bacteria in (88.1 ± 3.5)% of the
tests. However, in the other cases (11.9 ± 3.5)%, the fi-
nal result does not differ from the check experiments.
Only the irradiation dose 7.2 kGy results in the bacteri-
cidal effect.
In our further experiments, the results of the model
sample disinfection in small volumes have completely
coincided with the data concerning the irradiation of the
same test cultures in the volumes, substantially larger
(100 times). This testifies to the same nature of the
physical agent influence both on the cultures in small
and large pans.
The irradiation effect in the bacteriostatic and bacte-
ricidal regimes has been examined from the viewpoint
of detecting the microbes in the association, most resis-
tant to the beam influence. The investigations indicate
that the test-object reaction depends not on the species
of the bacteria in the model solutions but it is correlated
with the concentration of each kind of the bacteria, tak-
en into the investigations. The response reaction of 2-or
3-component microbial associations essentially differs
from the reaction of individual test cultures only in the
cases when their total concentration exceeds the concen-
tration of a single suspension. If the initial density (9 lg
CFU/ml) is preserved after the composition of the asso-
ciation, the beam aftereffect in compound model sam-
ples is the same as in the case of irradiating each mem-
ber of the association under the given density.
The data obtained testify that, for the microbial sus-
pension disinfection, low or medium intensities of the
beam are required. For the destruction of medicaments,
higher energy expenditures are necessary.
The beam absorbed doses within (3.6...7.2) kGy pro-
vide destruction of antibiotics in 19 of 36 samples ((52.8
± 8.3)%) with the antibiotic low concentration
(0.31...5.0) UA, the symbol UA marks “the units of ac-
tivity” and in 9 of 36 samples with higher concentration
of antimicrobial means ((10.0...100.0)UA).
0 5 1 0 1 5 20 25 30
0
20
40
60
80
100
%, UA
D, kGy
321
Fig.2. The beam influence on the antibiotics in the con-
centrations: 5 UA (1); 10 UA (2) and 100 UA (3)
The general pattern of the dependence of the degree
of antibiotic decomposition versus the irradiation dose
for various values of UA is given in Fig.2.
Heightening of the power load up to 15 kGy favors
neutralization of chemical therapeutic medicaments in
25 of 30 ((83.3 ± 6.8)%) model samples in contrast to
44 of 72 ((38.9 ± 5.7)%) samples, irradiated in the
above-mentioned regimes - i.e., twice as more often.
The further investigations have indicated that the irradi-
ation optimal regimes are achievable with the power
loads that provide absorbed doses from 30 kGy and
higher. In these regimes, antibiotics have been de-
stroyed in all the samples, including those with the
medicaments in high concentrations ((50.0...100.0)
UA).
190
4. CONCLUSIONS
Thus, it is demonstrated that the absorbed doses
(2.4...3.6) kGy make the threshold of the sanitary-model
florula sensitivity to the beam influence. The bacterici-
dal effect has been observed under the model sample ir-
radiation with the following doses: about 3.8 kGy for
enterobacteria (except Klebsiella, the dose for which
makes 10 kGy); 4.5 kGy for staphylococci and 4.8 kGy
for enterococci.
It is found out that there is no essential difference in
the character of antimicrobial effect between the beam
influence on small and large volumes of model samples
of the test cultures examined.
In the case of the irradiation of 2- and 3-component
associations of test cultures, their response reaction de-
pends on the final density of the model samples. If the
total concentration of microbial association does not ex-
ceed that of a monosuspension (109 CFU/ml), the bacte-
ricidal doses coincide with those that cause destruction
of the most resistant members of the association.
The beam absorbed dose 7.2 kGy decomposes an-
tibiotics (penicillin, streptomycin and tetracycline) in
the concentrations (0.31...5.0) UA. If the medicament
concentrations are higher (up to 100 UA), the chemical
compound is decomposed with the doses (15...30) kGy.
The work is fulfilled within Project #1971 under the fi-
nancial support given by STCU.
ВЛИЯНИЕ РЕЛЯТИВИСТСКИХ ЭЛЕКТРОНОВ НА САНИТАРНО-ПОКАЗАТЕЛЬНЫЕ
МИКРООРГАНИЗМЫ И АНТИБИОТИКИ В МОДЕЛЬНЫХ ОБРАЗЦАХ
В.С.Антипов, Е.М.Бабич, И.В.Бережная, Ю.Л.Волянский, О.Ю.Карпенко, О.Ф.Ковпик, Е.А.Корнилов,
В.А.Киселев, А.Ф.Линник, Ю.И.Сергиенко, Н.И.Скляр, В.В.Усков, Т.В.Хирная
Выполнен цикл исследований по влиянию электронного пучка на санитарно-показательные тест-культу-
ры и антибиотики в модельных растворах. Для каждого тест-объекта установлены предельные дозы погло-
щенного облучения, выше которых бактерицидное действие резко усиливается и становится полным. При
этом обеспечивается стабильность санитарно-бактериологических показателей образцов воды на протяже-
нии 6 дней. Показано, что обработка электронным пучком модельных образцов антибиотиков, взятых в кон-
центрациях до 100 ОА, в режимах до 30 кГр приводило к уменьшению и полной нейтрализации антибакте-
риальной активности во всех пробах образцов.
ВПЛИВ РЕЛЯТИВІСТСЬКИХ ЕЛЕКТРОНІВ НА САНІТАРНО-ПОКАЗОВІ
МІКРООРГАНІЗМИ ТА АНТИБІОТИКИ В МОДЕЛЬНИХ ЗРАЗКАХ
В.С.Антіпов, Є.М.Бабич, І.В.Бережна, Ю.Л.Волянський, О.Ю.Карпенко, О.Ф.Ковпік, Є.О.Корнілов,
В.О.Кисельов, А.Ф.Ліннік, Ю.І.Сергієнко, Н.І.Скляр, В.В.Усков, Т.В.Хірна
Виконано цикл досліджень по впливу електронного пучка на санітарно-показові тест-культури та
антибіотики в модельних розчинах.. Для кожного тест-об’єкта визначено граничні дози поглинутого
випромінювання, вище яких бактерицидна дія різко посилюється і стає повною. При цьому забезпечується
стабільність санітарно-бактеріологічних показників зразків води протягом 6 діб. Показано, що оброблення
електронним пучком модельних зразків антибіотиків, взятих в концентраціях до 100 ОА, в режимах до 30
кГр приводило до зменшення та повної нейтралізації антибактеріальної активності у всіх пробах зразків
___________________________________________________________
PROBLEMS OF ATOMIC SIENCE AND TECHNOLOGY. 2004. № 2.
Series: Nuclear Physics Investigations (43), p.189-191.
191
NSC Kharkov`s Institute of Physics & Technology, Kharkiv, Ukraine
1. INTRODUCTION
2. TECHNICAL APPROACH
4. CONCLUSIONS
|
| id | nasplib_isofts_kiev_ua-123456789-79393 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1562-6016 |
| language | English |
| last_indexed | 2025-12-07T17:16:50Z |
| publishDate | 2004 |
| publisher | Національний науковий центр «Харківський фізико-технічний інститут» НАН України |
| record_format | dspace |
| spelling | Antipov, V.S. Babych, E.M. Berezhna, I.V. Voliansky, Yu.L. Karpenko, O.Yu. Kovpik, O.F. Kornilov, E.O. Kiselev, V.A. Linnik, A.F. Sergienko, Yu.I. Sklar, N.I. Uskov, V.V. Hirna, T.V. 2015-03-31T19:25:50Z 2015-03-31T19:25:50Z 2004 Relativistic electron influence on sanitary-model microorganisms and antibiotics in model samples / V.S. Antipov, E.M. Babych, I.V. Berezhna, Yu.L. Voliansky, O.Yu. Karpenko*, O.F. Kovpik, E.O. Kornilov, V.A. Kiselev, A.F. Linnik, Yu.I. Sergienkо, N.I. Sklar, V.V. Uskov, T.V. Hirna // Вопросы атомной науки и техники. — 2004. — № 2. — С. 189-191. — англ. 1562-6016 PACS: 87.23.-n https://nasplib.isofts.kiev.ua/handle/123456789/79393 A series of the investigations of the electron beam influence on sanitary-model test cultures and antibiotics in
 model solutions has been carried out. For each of the test objects, the authors have found the boundary doses of the
 absorbed radiation. The higher doses cause the sharp increase in the bactericidal influence, which becomes complete. The sanitary-bactericidal indices of the water samples remain stable during 6 days. The samples of antibiotics
 in various concentrations (from 100 UA) have been irradiated. It is proved that the substratum processing by the
 beam (in the regimes 30 kGy) causes diminution and complete neutralization of the antibacterial activity in all
 probes of the samples. Виконано цикл досліджень по впливу електронного пучка на санітарно-показові тест-культури та
 антибіотики в модельних розчинах.. Для кожного тест-об’єкта визначено граничні дози поглинутого
 випромінювання, вище яких бактерицидна дія різко посилюється і стає повною. При цьому забезпечується
 стабільність санітарно-бактеріологічних показників зразків води протягом 6 діб. Показано, що оброблення
 електронним пучком модельних зразків антибіотиків, взятих в концентраціях до 100 ОА, в режимах до 30
 кГр приводило до зменшення та повної нейтралізації антибактеріальної активності у всіх пробах зразків. Выполнен цикл исследований по влиянию электронного пучка на санитарно-показательные тест-культуры и антибиотики в модельных растворах. Для каждого тест-объекта установлены предельные дозы поглощенного облучения, выше которых бактерицидное действие резко усиливается и становится полным. При
 этом обеспечивается стабильность санитарно-бактериологических показателей образцов воды на протяжении 6 дней. Показано, что обработка электронным пучком модельных образцов антибиотиков, взятых в концентрациях до 100 ОА, в режимах до 30 кГр приводило к уменьшению и полной нейтрализации антибактериальной активности во всех пробах образцов. The work is fulfilled within Project #1971 under the financial support given by STCU. en Національний науковий центр «Харківський фізико-технічний інститут» НАН України Вопросы атомной науки и техники Применение ускоренных пучков Relativistic electron influence on sanitary-model microorganisms and antibiotics in model samples Вплив релятивістських електронів на санітарно-показові мікроорганізми та антибіотики в модельних зразках Влияние релятивистских электронов на санитарно-показательные микроорганизмы и антибиотики в модельных образцах Article published earlier |
| spellingShingle | Relativistic electron influence on sanitary-model microorganisms and antibiotics in model samples Antipov, V.S. Babych, E.M. Berezhna, I.V. Voliansky, Yu.L. Karpenko, O.Yu. Kovpik, O.F. Kornilov, E.O. Kiselev, V.A. Linnik, A.F. Sergienko, Yu.I. Sklar, N.I. Uskov, V.V. Hirna, T.V. Применение ускоренных пучков |
| title | Relativistic electron influence on sanitary-model microorganisms and antibiotics in model samples |
| title_alt | Вплив релятивістських електронів на санітарно-показові мікроорганізми та антибіотики в модельних зразках Влияние релятивистских электронов на санитарно-показательные микроорганизмы и антибиотики в модельных образцах |
| title_full | Relativistic electron influence on sanitary-model microorganisms and antibiotics in model samples |
| title_fullStr | Relativistic electron influence on sanitary-model microorganisms and antibiotics in model samples |
| title_full_unstemmed | Relativistic electron influence on sanitary-model microorganisms and antibiotics in model samples |
| title_short | Relativistic electron influence on sanitary-model microorganisms and antibiotics in model samples |
| title_sort | relativistic electron influence on sanitary-model microorganisms and antibiotics in model samples |
| topic | Применение ускоренных пучков |
| topic_facet | Применение ускоренных пучков |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/79393 |
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