Photonuclear method of production Cu-67
The efficiently separation of ⁶⁷Cu is achieved by using diantipyrylpropylmethane (DАPPМ). Experimental separation of ⁶⁷Cu from sulphuric solution (1 mol./L) of zinc (2 mol./L) with addition potassium iodide (0.1 mol./L) is realized with help DAPPM (0.02 mol./L), which dissolve in chloroform. The 84...
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nasplib_isofts_kiev_ua-123456789-802812025-02-10T01:32:52Z Photonuclear method of production Cu-67 Aizatskyi, N.I. Dikiy, N.P. Dovbnya, A.N. Dolzhek, M.A. Lyashko, Yu.V. Medvedeva, E.P. Medvedev, D.V. Применение ускорителей в радиационных технологиях The efficiently separation of ⁶⁷Cu is achieved by using diantipyrylpropylmethane (DАPPМ). Experimental separation of ⁶⁷Cu from sulphuric solution (1 mol./L) of zinc (2 mol./L) with addition potassium iodide (0.1 mol./L) is realized with help DAPPM (0.02 mol./L), which dissolve in chloroform. The 84.4% ⁶⁷Сu was extracted from water phase of ZnSO₄ into organic phase (solution DAPPM in chloroform). The ⁶⁷Cu remainder in organic phase is 4.6%. Effective extraction of protonated forms of reagent and ionic associate of metal+halide was realized by means of antipyrin forms in acid halide solution. The decrease of acidity of a water phase is necessary for effective reextraction. Re-extraction was realised by means of consecutive washing of organic phase (DAPPM in chloroform) by means of distilled water. Some amount of ZnSO₄ in process of extraction gets in organic phase that causes a low level re-extraction in first two tests. These tests re-extraction have low values рН. Re-extraction ⁶⁷Cu from solution DAPPM in chloroform is carried out consistently four times by distilled water. The activity of ⁶⁷Cu in third and fourth re-extraction tests was 72.7%. Экстракция диантипирилпропилметаном (ДАППМ) позволяет эффективно выделять ⁶⁷Cu. Реализовано экспериментальное выделение ⁶⁷Cu из сернокислого раствора (1 моль/л) цинка (2 моль/л) с добавлением йодистого калия (0,1 моль/л) при помощи ДАППМ (0,02 моль/л), растворенного в хлороформе. При экстракции ⁶⁷Сu из водной фазы ZnSO₄ переходит в органическую фазу (раствор ДАППМ в хлороформе) 84,4% ⁶⁷Сu. В органической фазе остается 4,6% ⁶⁷Сu. Производные антипирина образуют в кислых галогенидных растворах хорошо экстрагирующиеся ионные ассоциаты металл + галогенидного аниона и протонированной формы реагента. Поэтому для эффективной реэкстракции необходимо снижение кислотности водной фазы, что и реализуется при последовательных перемешиваниях органической фазы (ДАППМ в хлороформе) с дистиллированной водой. Некоторое количество ZnSO₄ в процессе экстракции попадает в органическую фазу, что обуславливает низкую степень реэкстракции в первых двух пробах. Эти пробы реэкстракции имеют низкие значения рН. Реэкстракцию ⁶⁷Сu из раствора ДАППМ в хлороформе проводили последовательно четыре раза дистиллированной водой. В третью и четвертую пробы реэкстрагировалось 72,7% активности ⁶⁷Сu. Екстракція діантіпірілпропілметаном (ДАППМ) дозволяє ефективно виділяти ⁶⁷Cu. Реалізовано експериментальне виділення ⁶⁷Cu з сірчанокислого розчину (1 моль/л) цинку (2 моль/л) з додаванням йодистого калію (0,1 моль/л) за допомогою ДАППМ (0,02 моль/л), розчиненого в хлороформі. При екстракції ⁶⁷Cu з водної фази ZnSO₄ переходить в органічну фазу (розчин ДАППМ в хлороформі) 84,4% ⁶⁷Cu. У органічній фазі залишається 4,6% ⁶⁷Cu. Похідні антипірину утворюють у кислих галогенідних розчинах добре екстрагуючі іонні асоціати метал + галогенідні аніони і протоновану форми реагенту. Тому для ефективної реекстракції необхідне зниження кислотності водної фази, що і реалізується при послідовних перемішуваннях органічної фази (ДАППМ в хлороформі) з дистильованою водою. Деяка кількість ZnSO₄ в процесі екстракції потрапляє в органічну фазу, що обумовлює низьку ступінь реекстракції в перших двох пробах. Ці проби реекстракції мають низькі значення рН. Реекстракції ⁶⁷Cu з розчину ДАППМ у хлороформі проводили послідовно чотири рази дистильованою водою. У третю і четверту проби реекстрагувалося 72,7% активності ⁶⁷Cu 2014 Article Photonuclear method of production Cu-67 / N.I. Aizatskyi, N.P. Dikiy, A.N. Dovbnya, M.A. Dolzhek, Yu.V. Lyashko, E.P. Medvedeva, D.V. Medvedev // Вопросы атомной науки и техники. — 2014. — № 3. — С. 182-185. — Бібліогр.: 16 назв. — англ. 1562-6016 PACS: 28.60.+s; 87.53.Jw https://nasplib.isofts.kiev.ua/handle/123456789/80281 en Вопросы атомной науки и техники application/pdf Національний науковий центр «Харківський фізико-технічний інститут» НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
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| topic |
Применение ускорителей в радиационных технологиях Применение ускорителей в радиационных технологиях |
| spellingShingle |
Применение ускорителей в радиационных технологиях Применение ускорителей в радиационных технологиях Aizatskyi, N.I. Dikiy, N.P. Dovbnya, A.N. Dolzhek, M.A. Lyashko, Yu.V. Medvedeva, E.P. Medvedev, D.V. Photonuclear method of production Cu-67 Вопросы атомной науки и техники |
| description |
The efficiently separation of ⁶⁷Cu is achieved by using diantipyrylpropylmethane (DАPPМ). Experimental separation of ⁶⁷Cu from sulphuric solution (1 mol./L) of zinc (2 mol./L) with addition potassium iodide (0.1 mol./L) is realized with help DAPPM (0.02 mol./L), which dissolve in chloroform. The 84.4% ⁶⁷Сu was extracted from water phase of ZnSO₄ into organic phase (solution DAPPM in chloroform). The ⁶⁷Cu remainder in organic phase is 4.6%. Effective extraction of protonated forms of reagent and ionic associate of metal+halide was realized by means of antipyrin forms in acid halide solution. The decrease of acidity of a water phase is necessary for effective reextraction. Re-extraction was realised by means of consecutive washing of organic phase (DAPPM in chloroform) by means of distilled water. Some amount of ZnSO₄ in process of extraction gets in organic phase that causes a low level re-extraction in first two tests. These tests re-extraction have low values рН. Re-extraction ⁶⁷Cu from solution DAPPM in chloroform is carried out consistently four times by distilled water. The activity of ⁶⁷Cu in third and fourth re-extraction tests was 72.7%. |
| format |
Article |
| author |
Aizatskyi, N.I. Dikiy, N.P. Dovbnya, A.N. Dolzhek, M.A. Lyashko, Yu.V. Medvedeva, E.P. Medvedev, D.V. |
| author_facet |
Aizatskyi, N.I. Dikiy, N.P. Dovbnya, A.N. Dolzhek, M.A. Lyashko, Yu.V. Medvedeva, E.P. Medvedev, D.V. |
| author_sort |
Aizatskyi, N.I. |
| title |
Photonuclear method of production Cu-67 |
| title_short |
Photonuclear method of production Cu-67 |
| title_full |
Photonuclear method of production Cu-67 |
| title_fullStr |
Photonuclear method of production Cu-67 |
| title_full_unstemmed |
Photonuclear method of production Cu-67 |
| title_sort |
photonuclear method of production cu-67 |
| publisher |
Національний науковий центр «Харківський фізико-технічний інститут» НАН України |
| publishDate |
2014 |
| topic_facet |
Применение ускорителей в радиационных технологиях |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/80281 |
| citation_txt |
Photonuclear method of production Cu-67 / N.I. Aizatskyi, N.P. Dikiy, A.N. Dovbnya, M.A. Dolzhek, Yu.V. Lyashko, E.P. Medvedeva, D.V. Medvedev // Вопросы атомной науки и техники. — 2014. — № 3. — С. 182-185. — Бібліогр.: 16 назв. — англ. |
| series |
Вопросы атомной науки и техники |
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| first_indexed |
2025-12-02T12:13:51Z |
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2025-12-02T12:13:51Z |
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1850398612309147648 |
| fulltext |
ISSN 1562-6016. ВАНТ. 2014. №3(91) 182
PHOTONUCLEAR METHOD OF PRODUCTION Cu-67
N.I. Aizatskyi, N.P. Dikiy, A.N. Dovbnya, M.A. Dolzhek, Yu.V. Lyashko, E.P. Medvedeva,
D.V. Medvedev
National Science Center “Kharkov Institute of Physics and Technology”, Kharkov, Ukraine
E-mail: ndikiy@kipt.kharkov.ua
The efficiently separation of 67Cu is achieved by using diantipyrylpropylmethane (DАPPМ). Experimental sepa-
ration of 67Cu from sulphuric solution (1 mol./L) of zinc (2 mol./L) with addition potassium iodide (0.1 mol./L) is
realized with help DAPPM (0.02 mol./L), which dissolve in chloroform. The 84.4% 67Сu was extracted from water
phase of ZnSO4 into organic phase (solution DAPPM in chloroform). The 67Cu remainder in organic phase is 4.6%.
Effective extraction of protonated forms of reagent and ionic associate of metal+halide was realized by means of
antipyrin forms in acid halide solution. The decrease of acidity of a water phase is necessary for effective re-
extraction. Re-extraction was realised by means of consecutive washing of organic phase (DAPPM in chloroform)
by means of distilled water. Some amount of ZnSO4 in process of extraction gets in organic phase that causes a low
level re-extraction in first two tests. These tests re-extraction have low values рН. Re-extraction 67Cu from solution
DAPPM in chloroform is carried out consistently four times by distilled water. The activity of 67Cu in third and
fourth re-extraction tests was 72.7%.
PACS: 28.60.+s; 87.53.Jw
INTRODUCTION
Copper is the irreplaceable microelement necessary
for normal vital functions of the person. Copper is main-
ly involved in redox reactions throughout the body, but
also plays a role in iron transportation in blood plasma.
Copper is part of hormones and influences growth, de-
velopment, reproduction, an exchange, processes of
formation of haemoglobin, phagocytic activity of leuko-
cytes.
67Cu is one of the most promising isotopes for radio-
immunotherapy. The half-value period of 67Cu is 61.5 h
and it well matched to the residence time of a typical
antibody on the tumour. 67Cu emit β-particles as well as
a moderate abundance of 93 and 184 keV gamma rays.
These gamma rays permit radiation dosimetry to be pre-
dicted from the information obtained from quantitative
imaging studies on pretherapy doses of 67Cu. This
pretherapy imaging and kinetics, followed by similar
pharmacokinetics of the actual therapy doses, allows
treatment planning and on-going verification of that
planning with actual dosimetric information on the radi-
ation delivered.
Various ways of use of 67Cu for therapy and diag-
nostics of cancer diseases are used. For example ethyl-
glyoxal bis(thiosemicarbazone) has potential utility as
radiopharmaceutical with the various isotopes of copper
[1]. 64Cu TETA-Octreotide is a chelate that has been
shown to bind to the somatostatin receptor [2]. CB-
TE2A a stable chelation system for 67Cu was incorpo-
rated with Bombesin analogs for in vitro and in vivo
studies of prostate cancer [3]. 67Cu-ATSM (diacetyl-bis
(N4-methylthiosemicarbazone)) has been shown to in-
crease the survival time of tumor-bearing animals with
no acute toxicity [4]. 67Cu is widely used in radioim-
munoassay therapy and in early diagnosis and treatment
of lung cancer. For this purpose the unicellular suspen-
sion was treated by solution 5, 10, 15, 20-tetrakis (4-
carboxyphenyl) porphine for a time sufficient to sub-
stantially capture by malignant cells. The treated cell
suspension was irradiated by UV irradiation. Diagnosis
of malignant lung cells realize by the number of fluo-
rescent cells. The localization of malignant process of
lung in the whole organism was performed by means of
introducing in a patient's blood of porphyrin derivatives
with subsequent analysis of the image produced in the
emitted gamma radiation. In the case of treatment of the
patient is administered complex 5, 10, 15, 20-tetrakis
(4-carboxyphenyl)-67Cu-(II) porphine in the form of an
aerosol.
Low cost and high specific activity is necessary
conditions of wide use of 67Cu in nuclear medicine.
NSC KIPT is developing a process for production of
67Cu using an electron linac. The two most significant
criteria are the specific activity of the 67Cu and the ab-
sence of other metals that could compete with the chelat-
ing agent that medical researchers plan to use to target the
delivery of the 67Cu. Moreover yield and waste disposal
requirements necessitate the use of an isotopically en-
riched target, which must be recovered and reused.
The purpose of the present work is development of
method of production of 67Cu with low cost, with high
specific activity and absence of metal impurity in solution.
RESULTS AND DISCUSSION
One of effective ways of separation of 67Cu is a
method internal electrolysis, which allows receiving
pure copper without the carrier [5]. Recently the 67Cu
finds more and more wide use for treatment of tumours
[6]. The significant number of articles [7 - 13] is devot-
ed to development of methods of synthesis 67Cu. Most
frequently at manufacture of isotopes of copper is used
extraction and electrochemical sedimentation.
Extraction by organic reagents allows realizing au-
tomation of the separation of the radioactive isotope.
Extraction by acetone [7] provides the attitude of distri-
bution approximately 100 that does not satisfy to condi-
tions for further use 67Cu as pharmaceutical production.
Using diantipyrylpropylmethane provides a distribution
coefficient more than 103 copper and about 10-4 for zinc,
which corresponds to a ratio of zinc for initial and final
solution 10-7 [14 - 15].
Metal zinc, zinc oxide from closed Joint-stock com-
pany "OEZ" “VladMiVa", chemically pure sulfuric ac-
id, pure hydrochloric acid, chemically pure chloroform,
http://en.wikipedia.org/wiki/Redox
ISSN 1562-6016. ВАНТ. 2014. №3(91) 183
chemically pure (PDS 2930-0002), diantipyrylpropyl-
methane (DAPPM) (С26Н30N4О2) of analytical grade,
dithizone (diphenylthiocarbazone, C13H11N4S), reagent
grade, TU 6-09-07-1684, Pyrex glass, Viton tubing
(Cole Parmer Instrument, Vernon Hills), connecting
silicone a tube had been used at fulfillment of investiga-
tions.
Series of experiments about dissolution of zinc metal
and zinc oxide was carried out. This procedure is given
special attention, because dissolving the irradiated zinc
should take place in the shortest possible time and in a
special container made of glass, not containing in the
structure of that element. This procedure is being per-
formed in a hot cell and should be as simple as possible.
The sample of metal zinc (998 mg) was placed in a bowl
with a solution of hydrochloric acid (HCl) which was
diluted (1:1). After dilution of zinc the hydrochloric acid
was evaporated with addition of 0.6 ml of sulfuric acid to
a final volume of aqueous phase of Н2SO4 with concen-
tration 1…2 mol./L of zinc. Solution was heated until
vapours of sulfuric acid and complete dilution of sample.
The resulting solution has a transparent lemon colour.
For simplification of procedure of dissolution was
used zinc oxide (manufactured by JSC "OEZ"
“VladMiVa") in an amount of 8 g. The 7 ml of concen-
trate sulfuric acid was added in a bowl and was added
~20 ml of distilled water. Heating of a bowl carried out
before full dissolution of the zinc sample. In order to
enhance the dissolution of zinc in bowl was added a
50% solution of hydrogen peroxide.
The most suitable concentration of hydrogen ions for
dilution of zinc is in the range from pH 2 to 3. In the
case when metallic zinc after adding sulfuric acid is not
completely dissolved it was necessary to add to a cold
solution of hydrogen peroxide. Then a mix of solutions
in addition warmed up.
For obtaining isotope 67Cu the technique of extrac-
tion from iodide system is developed. In this case potas-
sium iodide is being entered into a solution of sulfuric
acid. In sour iodide solutions the copper is present at a
degree of oxidation (+1), forming strong anionic com-
plexes CuI2¯. Their stability is much more than of zinc
(ІІ). Therefore in this system the greatest contrast is
being observed at extraction of copper concerning zinc.
Optimum pH for the separation of copper from the zinc
is equal to 1 mol./L for Н2SО4 and 0.1 mol./L for KI. In
these conditions the copper extraction was realized in
form of ionic associate CuI2¯ with protonate form of
reagent LH+. In less sour area the significant contribu-
tion of copper as introduction complex creates difficul-
ties in re-extraction.
The important condition of extraction of copper is
the ratio of concentration of reagent L and iodine-ions.
Extraction is optimum at 2-3 multiple surplus of I¯ in
relation to L. In these conditions K copper exceeds 103,
and for zinc it is less 10-3. Falling of extraction of cop-
per at [L]>[I−] is caused by destruction of iodine com-
plex of copper by reagent surplus:
CuI2ˉ + 2LH+ → Cu+ + LHI. (1)
к1
к2
к3
к4
к5
к6
к7
condenser
extractor,
CHCl3,
DAPPM evaporation
bowl 6
heater
Sol. ZnSO4,
H2SO4, КI
Re-extractor,
DAPPM
Н2О
peristaltic
pump
peristaltic pump
compressor
Assembly
of solution
heater
Н2О
Н2О
1 2
3 4 5
Solution KI
Solution
67Cu
1 sample re-extraction
Н2SO4, Н2O2, H2O CHCl3, DAPPM
tap 1,2
tap 3,4
waste Zn, Н2SO4 waste CHCl3, DAPPM
tap 5
control panel
1. pomps (к1-к7)
2. 2 heater
3. instrument illumination
4. 2 peristaltic pump
Waste
Н2О
Fig. 1. The scheme of installation
Reduction of copper extraction at [L]<[I−] is caused
by deficiency of a reagent owing to his linkage in ionic
associate with an iodine-ion:
LHICuI2 + I → LHI + CuI2¯. (2)
At significant concentration of zinc (to 2 mol./L) the
factor of distribution Kp of copper does not change.
Also down to concentration of copper 10−3 mol./L Kp of
copper remains constant. Essential feature of use of the
given system is simplicity re-extraction copper. De-
crease of acidity of an organic phase by water results to
effective re-extraction copper. The sulfuric water phase
with concentration of a sulfuric acid 1 mol./l, of zinc
2 mol./l and of potassium iodide 0.1 mol./l was located
in separating funnel (2) (Fig. 1). In extraction column
ISSN 1562-6016. ВАНТ. 2014. №3(91) 184
(1) solution DAPPM in chloroform with concentration
0.02 mol./L was located. Volumes of chloroform and a
water phase were equal (130 ml) and allowed to dissolu-
tion 20 g of metal zinc. Extraction of copper-67 was
carried out by pass of water phase through a capillary
extraction column with the help peristaltic pump. Speed
of swapping of a water phase is 13 ml/minute. The wa-
ter passed through solution DAPPM in chloroform and
again arrives in separating funnel (2). Time of extrac-
tion of copper-67 was 20 minutes. After the termination
of extraction the solution DAPPM in chloroform was
transported in extraction column (3). In separating fun-
nel (4) was poured 130 ml of distilled water and was
included peristaltic pump (7), which pumped water
through a capillary extraction column (3) within
10 minutes. In this case the water rose upwards and ar-
rived in separating funnel (5). The first test of a contact
mix lowers acidity of a solution up to рН=2, that creates
necessary conditions about acidity for transition of cop-
per-67 in the subsequent re-extracts. The first test of a
contact mix merges in a glass for waste products. Then
in extraction column (3) 130 ml of distilled water are
again added and the cycle re-extraction of copper-67 is
carried out. After that re-extract from separating funnel
(4) with the help peristaltic pump (10) was pumped over
in a flask (9). In a result it is carried out four consecu-
tive cycles re-extraction, which transported in a contact
solution more than 90% of copper-67.
The isotope 67Cu allows carry out therapy of many
cancer diseases. Extraction diantipyrylpropylmethane
(DAPPM) allows to effectively separation of 67Cu. Ex-
perimental separation 67Cu from sulfuric solution
(1 mol./L) zinc (2 mol./L) with addition potassium io-
dide (0.1 mol./L) is realized with help (DAPPM
(0.02 mol./L), dissolved in chloroform. At extraction of
67Cu from water phase ZnSO4 passes in an organic
phase (solution DAPPM in chloroform) 84.4% 67Сu
(Table).
Activity and the content of impurity in re-extract
№
sample
Yield
67Сu,
%
рН
solution
re-
extract
The share of
activity 65Zn
in re-extract
Content
Zn
in re-
extract,
µg/ml
Content
Cu
in re-
extract,
µg/ml
1 0.7 2 < 2⋅10−4 20.8 0.0757
2 0.8 2.5 < 6⋅10−5 3.92 0.132
3 33.7 4.5 < 2⋅10−5 0.45 0.0052
4 39.0 5 < 2⋅10−5 0.05 0.0045
5* 5.6 7 < 2⋅10−5 − −
6** 4.6 − < 2⋅10−5 − −
5* − solution NaOH for quantitative re-extraction;
6** − solution DAPPM in CHCl3 after re-extraction.
The activity of 67Cu in organic phase is 4.6%. Deriv-
atives of antipyrine are producing in acidic halide solu-
tions ion associates well extractable metal-halide anions
and the protonated forms of the reagent. Therefore, the
basic method of re-extraction is a reduction in acidity of
the aqueous phase by mixing the organic phase with an
alkaline solution or water. The destruction of the copper
iodide is achieved by the introduction of excess reagent
DAPPM. Some amount ZnSO4 in process extraction
gets in an organic phase that causes a low degree re-
extraction in first two tests. These tests re-extraction
have low values рН.
The activity of initial solution ZnSO4, of organic
phase and of tests re-extract was measured by Ge(Li)-
detector in volume of 40 cm3 with the power resolution
3.2 by 1332 keV (Fig. 2).
Re-extraction of 67Сu from solution DAPPM in
chloroform was carried out consistently four times by
distilled water (see Table). In third and fourth tests were
re-extracted 72.7% of activity 67Сu (Fig. 3). The fifth re-
extraction of 67Сu was carried out by quantitatively a
solution of caustic soda (рН=7) and its activity was
5.6% from initial activity of 67Сu.
200 400 600 800 1000 1200 1400
101
102
103
104
67Cu 93 keV
co
un
ts
number channel
65Zn 1115 keV
511 keV
67Cu 184 keV
Fig. 2. The spectrum of solution after irradiation of Zn
200 400 600 800 1000 1200 1400
101
102
103
104
105
67Cu 394 keV
67Cu 300 keV
67Cu 93 keV
67Cu 184 keV
511 keV
65Zn 1115 keV
co
un
ts
number channel
Fig. 3. The spectrum of solution after extraction of 67Cu
Use of 67Cu for radio immune therapy shows the cer-
tain requirements about the content of zinc in re-extract.
The content of zinc in re-extract of copper should not
exceed a level of copper. Therefore for the further
downturn of the content of zinc in re-extract is supposed
to use silica gel as an absorber [16]. Sorption of zinc by
silica gel is realized at рН=7. Therefore pass of re-
extraction through activated silica gel will allow reach
the set content of zinc in a preparation of 67Cu which
can be used for radio immune therapy. Research of in-
fluence of masking of zinc for increase in factors of
distribution of copper [13] and reduction of factor of
distribution for zinc is supposed also.
ISSN 1562-6016. ВАНТ. 2014. №3(91) 185
CONCLUSIONS
1. The model of installation for extraction of copper-67
with use diantipyrylpropylmethane is created and tested.
2. The ratio of the content of zinc in re-extract in rela-
tion to initial zinc 3⋅10−6 is received.
3. The extraction of copper is achieved at level of 90%.
REFERENCES
1. M.A. Green, C.J. Mathia, L.R. Willi, et al. Assess-
ment of Cu-ETS as a PET radiopharmaceutical for
evaluation of regional renal perfusion // Nuclear
Medicine and Biology. 2007, №3, p. 247-255.
2. L.A. Bass, M. Wang, M.J. Welch, et al. In Vivo
Transchelation of Copper-64 from TETA-Octreotide
to Superoxide Dismutase in Rat Liver // Bioconju-
gate Chemistry. 2000, p. 527-532.
3. J.J. Parry, R. Andrews, B.E. Rogers. MicroPET im-
aging of breast cancer using radiolabeled bombesin
analogs targeting the gastrin-releasing peptide recep-
tor // Springer. 2007, p.175-183.
4. J.S. Lewis, R. Laforest, T.L. Buettner, et al. Copper-
64-diacetyl-bis(N4-methylthiosemicarbazone): An
agent for radiotherapy // Proceedings of the National
Academy of Sciencies. 2001, p.1206-1211.
5. B.Z. Iofa, G.M. Dakar, N.M. Kolesnikova. Methods of
reception and measurement of radioactive preparations,
collected articles. М.: “Atomizdat”. 1960, p. 278-333.
6. A.A. Shishkin, A.G. Ivanov, A.V. Zajtsev, et al.
Copperinclusive monokarboranilporfin the prototype
new DNA-connecting cytotoxic compound // DAN.
2005, №6, p. 833-836 (in Russian).
7. P. Polak, J. Geraldts, R. Vlist, L. Lindner. Photo-
nuclear production of 67Cu from targets // Radi-
ochimica Acta. 1986, p. 169-173.
8. R. Schwarzbach, K. Zimmermann, P. Blauenstein, et
al. Development of a simple and selective separation
of 67Cu from irradiated zinc for use in antibody la-
beling: a comparison of methods // Appl. Radiat.
Isot. 1995, №5, p. 329-336.
9. H. Itabashi, Y. Shigeta, H. Kawamoto, H. Akaiwa.
Simultaneous determination of the complexing ca-
pacity and conditional stability constant of soluble
copper (II) complexes in natural-water samples by
using a chelate extraction technique // Anal. Scien.
2000, p. 1179-1182.
10. I.E. Alekseev, V.V. Darmogray, N.S. Marchenko.
Development of diffusion-thermal technologies for
67Сu and 124I for radionuclide therapy and positron
emission tomography // Radiochemistry. 2005, № 5,
p. 460-466.
11. S.I. Bondarevskiy, V.V. Eremin, F.S. Nasredinov.
Separation of radionuclides from irradiated metal
matrices with high "dry" methods // Radiochemistry.
2002, № 1, p. 45-48.
12. M. Yagi, K.J. Kondo. Preparation of carrier-free
67Cu by the 68Zn(γ,p) reaction // Int. jour. Appl. Rad.
Isot. 1978, p. 757-759.
13. K. Watanabe, T. Tanaka, A. Iburaim, M. Itagaki.
Effects of masking agents on the separation of copper
(II) from iron (III) by continuous extraction with 8-
hydroxyquinoline // Anal. Sci. 2001, p. 671-674.
14. B.I. Petrov. Diantipyrylmethane as extraction reagents
// JAKh. (ХХХУIII). 1983, iss.11, p. 2051-2077.
15. A.N. Planovsky, V.M. Ramm, S.Z. Kagan. Process-
es and devices of chemical technologies. M.:
“Goskhimizdat”. 1962, 322 p.
16. V.N. Podchaynova, L.N. Simonova. Copper. M.:
“Nauka”. 1990, 279 p.
Article received 29.09.2013
ФОТОЯДЕРНЫЙ МЕТОД ПРОИЗВОДСТВА Cu-67
Н.И. Айзацкий, Н.П. Дикий, A.Н. Довбня, М.А. Должек, Ю.В. Ляшко, Е.П. Медведева, Д.В. Медведев
Экстракция диантипирилпропилметаном (ДАППМ) позволяет эффективно выделять 67Cu. Реализовано
экспериментальное выделение 67Cu из сернокислого раствора (1 моль/л) цинка (2 моль/л) с добавлением йо-
дистого калия (0,1 моль/л) при помощи ДАППМ (0,02 моль/л), растворенного в хлороформе. При экстрак-
ции 67Сu из водной фазы ZnSO4 переходит в органическую фазу (раствор ДАППМ в хлороформе) 84,4%
67Сu. В органической фазе остается 4,6% 67Сu. Производные антипирина образуют в кислых галогенидных
растворах хорошо экстрагирующиеся ионные ассоциаты металл + галогенидного аниона и протонированной
формы реагента. Поэтому для эффективной реэкстракции необходимо снижение кислотности водной фазы,
что и реализуется при последовательных перемешиваниях органической фазы (ДАППМ в хлороформе) с ди-
стиллированной водой. Некоторое количество ZnSO4 в процессе экстракции попадает в органическую фазу,
что обуславливает низкую степень реэкстракции в первых двух пробах. Эти пробы реэкстракции имеют низ-
кие значения рН. Реэкстракцию 67Сu из раствора ДАППМ в хлороформе проводили последовательно четыре
раза дистиллированной водой. В третью и четвертую пробы реэкстрагировалось 72,7% активности 67Сu.
ФОТОЯДЕРНИЙ МЕТОД ВИРОБНИЦТВА Cu-67
М.І. Айзацький, М.П. Дикий, A.М. Довбня, М.О. Должек, Ю.В. Ляшко, О.П. Медведєва, Д.В. Медведєв
Екстракція діантіпірілпропілметаном (ДАППМ) дозволяє ефективно виділяти 67Cu. Реалізовано експери-
ментальне виділення 67Cu з сірчанокислого розчину (1 моль/л) цинку (2 моль/л) з додаванням йодистого ка-
лію (0,1 моль/л) за допомогою ДАППМ (0,02 моль/л), розчиненого в хлороформі. При екстракції 67Cu з вод-
ної фази ZnSO4 переходить в органічну фазу (розчин ДАППМ в хлороформі) 84,4% 67Cu. У органічній фазі
залишається 4,6% 67Cu. Похідні антипірину утворюють у кислих галогенідних розчинах добре екстрагуючі
іонні асоціати метал + галогенідні аніони і протоновану форми реагенту. Тому для ефективної реекстракції
необхідне зниження кислотності водної фази, що і реалізується при послідовних перемішуваннях органічної
фази (ДАППМ в хлороформі) з дистильованою водою. Деяка кількість ZnSO4 в процесі екстракції потрапляє
в органічну фазу, що обумовлює низьку ступінь реекстракції в перших двох пробах. Ці проби реекстракції
мають низькі значення рН. Реекстракції 67Cu з розчину ДАППМ у хлороформі проводили послідовно чотири
рази дистильованою водою. У третю і четверту проби реекстрагувалося 72,7% активності 67Cu.
http://www.ncbi.nlm.nih.gov/pubmed?term=Parry%20JJ%5BAuthor%5D&cauthor=true&cauthor_uid=16838112
http://www.ncbi.nlm.nih.gov/pubmed?term=Andrews%20R%5BAuthor%5D&cauthor=true&cauthor_uid=16838112
http://www.ncbi.nlm.nih.gov/pubmed?term=Rogers%20BE%5BAuthor%5D&cauthor=true&cauthor_uid=16838112
http://www.pnas.org/search?author1=Richard+Laforest&sortspec=date&submit=Submit
http://www.pnas.org/search?author1=Thomas+L.+Buettner&sortspec=date&submit=Submit
INTRODUCTION
CONCLUSIONS
references
ФОТОЯДЕРНЫЙ МЕТОД ПРОИЗВОДСТВА Cu-67
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