Purification of recombinant GFP produced by Agrobacterum-mediated transient expression in Nicotiana excelsior

• Date: 2008
• Main Authors: Sindarovska, Y.R., Sheludko, Y.V., Gerasymenko, I.M., Bannikova, M.A., Kuchuk, N.V.
• Format: Article
• Language: English
• Published: Інститут клітинної біології та генетичної інженерії НАН України 2008
• Subjects: Оригинальные работы
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/8079
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Purification of recombinant GFP produced by Agrobacterum-mediated transient expression in Nicotiana excelsior / Y.R. Sindarovska, Y.V. Sheludko, I.M. Gerasymenko, M.A. Bannikova, N.V. Kuchuk // Цитология и генетика. — 2008. — Т. 42, № 2. — С. 16-20. — Бібліогр.: 20 назв. — англ.

Green fluorescent protein (GFP) is commonly used as a reporter protein in a wide range of biological experiments. The efficient protocol of Agrobacterium mediated transient expression in Nicotiana excelsior was applied for quick preparative production of recombinant GFP. The protein purification sch...