Chimerism and multiple numerical chromosome imbalances in a spontaneously aborted fetus

• Опубліковано в: : Цитология и генетика
• Дата: 2006
• Автори: Vorsanova, S.G., Iourov, I.Y., Demidova, I.A., Kirillova, E.A., Soloviev, I.V., Yurov, Y.B.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут клітинної біології та генетичної інженерії НАН України 2006
• Теми: Оригинальные работы
• Онлайн доступ: https://nasplib.isofts.kiev.ua/handle/123456789/82073
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Chimerism and multiple numerical chromosome imbalances in a spontaneously aborted fetus / S.G. Vorsanova, I.Y. Iourov, I.A. Demidova, E.A. Kirillova, I.V. Soloviev, Y.B. Yurov // Цитология и генетика. — 2006. — Т. 40, № 5. — С. 28-30. — Бібліогр.: 7 назв. — англ.

We report on a case of chimerism and multiple abnormalities of chromosomes 21, X and Y in spontaneous abortion specimen. To the best our knowledge the present case is the first documented chimera in a spontaneously aborted fetus. The application of interphase fluorescence in situ hybridization (FISH) using chromosome enumeration and site-specific DNA probes showed trisomy X in 92 nuclei (23 %), tetrasomy X in 100 nuclei (25 %), pentasomy of chromosome X in 40 nuclei (10 %), XXY in 36 nuclei (9 %), XXXXXXYY in 12 nuclei (3 %), XXXXXYYYYY in 8 nuclei (2 %), trisomy 21 and female chromosome complement in 40 nuclei (10 %), normal female chromosome complement in 72 nuclei (18 %) out of 400 nuclei scored. Our experience indicates that the frequency of chimerism coupled with multiple chromosome abnormalities should be no less than 1 : 400 among spontaneous abortions. The difficulties of chimerism identification in fetal tissues are discussed.