Determination of isotopic ratio of сalcium by gamma activation analysis and free radical oxidation of lipids in normal and cancerous tissues of thyroid gland
Chemiluminescence and gamma activation analysis was used to determine the isotopic ratio of calcium in normal, non-malignant and cancerous tissues of thyroid gland. This result was used for diagnostics of cancerous thyroid gland morbidity. The high value of isotopic ratio ⁴⁴Ca/⁴⁸Ca in paratumor tiss...
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| Zitieren: | Determination of isotopic ratio of сalcium by gamma activation analysis and free radical oxidation of lipids in normal and cancerous tissues of thyroid gland / N.P. Dikiy, A.N. Dovbnya, Е.P. Меdvedeva, N.A. Khizhnyak, V.L. Uvarov, A.A. Khizhnyak, N.V. Krasnoselsky // Вопросы атомной науки и техники. — 2000. — № 2. — С. 62-63. — Бібліогр.: 8 назв. — англ. |
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nasplib_isofts_kiev_ua-123456789-822752025-02-09T17:53:59Z Determination of isotopic ratio of сalcium by gamma activation analysis and free radical oxidation of lipids in normal and cancerous tissues of thyroid gland Определение изотопного соотношения кальция гамма активационным анализом и свободно-радикальное окисление липидов в нормальных и опухолевых тканях щитовидной железы Dikiy, N.P. Dovbnya, A.N. Меdvedeva, Е.P. Khizhnyak, N.A. Uvarov, V.L. Khizhnyak, A.A. Krasnoselsky, N.V. Аpplication of the nuclear methods Chemiluminescence and gamma activation analysis was used to determine the isotopic ratio of calcium in normal, non-malignant and cancerous tissues of thyroid gland. This result was used for diagnostics of cancerous thyroid gland morbidity. The high value of isotopic ratio ⁴⁴Ca/⁴⁸Ca in paratumor tissues of thyroid gland has allowed concluding about significance of transmembrane circulation calcium. 2000 Article Determination of isotopic ratio of сalcium by gamma activation analysis and free radical oxidation of lipids in normal and cancerous tissues of thyroid gland / N.P. Dikiy, A.N. Dovbnya, Е.P. Меdvedeva, N.A. Khizhnyak, V.L. Uvarov, A.A. Khizhnyak, N.V. Krasnoselsky // Вопросы атомной науки и техники. — 2000. — № 2. — С. 62-63. — Бібліогр.: 8 назв. — англ. 1562-6016 PACS: 87.58.Ji https://nasplib.isofts.kiev.ua/handle/123456789/82275 en Вопросы атомной науки и техники application/pdf Національний науковий центр «Харківський фізико-технічний інститут» НАН України |
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Аpplication of the nuclear methods Аpplication of the nuclear methods |
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Аpplication of the nuclear methods Аpplication of the nuclear methods Dikiy, N.P. Dovbnya, A.N. Меdvedeva, Е.P. Khizhnyak, N.A. Uvarov, V.L. Khizhnyak, A.A. Krasnoselsky, N.V. Determination of isotopic ratio of сalcium by gamma activation analysis and free radical oxidation of lipids in normal and cancerous tissues of thyroid gland Вопросы атомной науки и техники |
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Chemiluminescence and gamma activation analysis was used to determine the isotopic ratio of calcium in normal, non-malignant and cancerous tissues of thyroid gland. This result was used for diagnostics of cancerous thyroid gland morbidity. The high value of isotopic ratio ⁴⁴Ca/⁴⁸Ca in paratumor tissues of thyroid gland has allowed concluding about significance of transmembrane circulation calcium. |
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Article |
| author |
Dikiy, N.P. Dovbnya, A.N. Меdvedeva, Е.P. Khizhnyak, N.A. Uvarov, V.L. Khizhnyak, A.A. Krasnoselsky, N.V. |
| author_facet |
Dikiy, N.P. Dovbnya, A.N. Меdvedeva, Е.P. Khizhnyak, N.A. Uvarov, V.L. Khizhnyak, A.A. Krasnoselsky, N.V. |
| author_sort |
Dikiy, N.P. |
| title |
Determination of isotopic ratio of сalcium by gamma activation analysis and free radical oxidation of lipids in normal and cancerous tissues of thyroid gland |
| title_short |
Determination of isotopic ratio of сalcium by gamma activation analysis and free radical oxidation of lipids in normal and cancerous tissues of thyroid gland |
| title_full |
Determination of isotopic ratio of сalcium by gamma activation analysis and free radical oxidation of lipids in normal and cancerous tissues of thyroid gland |
| title_fullStr |
Determination of isotopic ratio of сalcium by gamma activation analysis and free radical oxidation of lipids in normal and cancerous tissues of thyroid gland |
| title_full_unstemmed |
Determination of isotopic ratio of сalcium by gamma activation analysis and free radical oxidation of lipids in normal and cancerous tissues of thyroid gland |
| title_sort |
determination of isotopic ratio of сalcium by gamma activation analysis and free radical oxidation of lipids in normal and cancerous tissues of thyroid gland |
| publisher |
Національний науковий центр «Харківський фізико-технічний інститут» НАН України |
| publishDate |
2000 |
| topic_facet |
Аpplication of the nuclear methods |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/82275 |
| citation_txt |
Determination of isotopic ratio of сalcium by gamma activation analysis and free radical oxidation of lipids in normal and cancerous tissues of thyroid gland / N.P. Dikiy, A.N. Dovbnya, Е.P. Меdvedeva, N.A. Khizhnyak, V.L. Uvarov, A.A. Khizhnyak, N.V. Krasnoselsky // Вопросы атомной науки и техники. — 2000. — № 2. — С. 62-63. — Бібліогр.: 8 назв. — англ. |
| series |
Вопросы атомной науки и техники |
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| fulltext |
DETERMINATION OF ISOTOPIC RATIO OF СALCIUM BY GAMMA
ACTIVATION ANALYSIS AND FREE RADICAL OXIDATION OF LIP-
IDS IN NORMAL AND CANCEROUS TISSUES OF THYROID GLAND
N.P. Dikiy, A.N. Dovbnya, Е.P. Меdvedeva, N.A. Khizhnyak, V.L. Uvarov
National Science Center “Kharkov Institute of Physics and Technology”, Kharkov, Ukraine
A.A. Khizhnyak, N.V. Krasnoselsky
Kharkov State Medical University, Kharkov, Ukraine
Chemiluminescence and gamma activation analysis was used to determine the isotopic ratio of calcium in normal,
non-malignant and cancerous tissues of thyroid gland. This result was used for diagnostics of cancerous thyroid
gland morbidity. The high value of isotopic ratio 44Ca/48Ca in paratumor tissues of thyroid gland has allowed con-
cluding about significance of transmembrane circulation calcium.
PACS: 87.58.Ji
INTRODUCTION
The influence of isolated and combined factors of ra-
diation, physical, chemical and biological nature on the
human organism promotes occurrence of different an-
omalies in the organism and, in particular, in the thyroid
gland. The pathogeny of the given disease is extremely
combined and is investigated insufficiently. Thus the
leading role of an autoimmune component is not called
in question [1]. Individual features of the person (hor-
monal, sexual, and emotional) and specificity of meta-
bolic processes proceeding in the human organisms in
different ecological regions stipulate the functional
activity of thyroid gland. Analysis of available statistical
data shows that there is the threshold quantity of atmo-
sphere pollution leading to activation of adaptive poten-
tialities of organism, and, consequently, to reduction of
the rate of thyroid gland cancer. The majority of sci-
entific research devoted to diagnostics, search of tumor-
al markers, problems of treatment and subsequent
checking the patients with thyroid gland cancer, includes
the whole complex of the tests [2]. However, despite of
considerable number of publications, unique answers to
many problems till now are not obtained.
The choice of necessary treatment is possible only at
valid diagnosis. The majority of research devoted to dia-
gnostics and searching markers of cancerous process
and the subsequent control of treatment efficiency is
possible with application of the whole complex of exam-
inations.
For the last two decades the rate of nodal formations
in thyroid gland is observed: for children in tens times,
for adults in 3-4 times. There are modern methods (son-
oradiography, X-ray computer tomography, nuclear-
magnetic resonance tomography, and the radioindicator
examinations), giving estimation of available changes.
But all of them do not satisfy completely the clinicians.
The opportunity of differential diagnostics of malignant
and non-malignant process in tissues of thyroid gland is
a problem and requires heightened attention to develop-
ment and upgrading of a system of diagnostics.
The facts of calcium level disturbance in blood are
known in the case of thyroid pathology [3]. The calcium
is universal intermediary during transmit of endocellular
signals from the outside and influences on the character
of exchange processes. The increase of calcium concen-
tration near the membrane represents a long-term calci-
um signal, which essentially changes the endocellular
control cascade, promotes disturbance of barrier func-
tion of membranes for Са2+ ions and can influence on
the induction of peroxide oxidation of lipids.
The purpose of the paper is to study the isotopic ra-
tio of calcium and intensity of the free radical peroxide
oxidation of lipids in normal, paratumor tissues and ma-
lignant tissues of thyroid gland.
METHODS
For measuring the isotopic ratio of calcium the activ-
ation analysis by bremsstrahlung radiation from the elec-
tron accelerator R&D "Accelerator" was used. After ir-
radiation the isotopic ratio of calcium was measured
with a Ge(Li)) - detector (volume 50 cm3, energy resolu-
tion 2.2 keV on line 1333 keV). The nuclear reactions
48Ca(γ,n)47Са, 44Ca(γ,p)43K induced by a bremsstrahlung
radiation and photoneutrons from the electron accelerat-
or with Е = 25 МeV and current 700 µА were used.
The method of chemiluminescence is a direct highly
sensitive method for determining electron-excited states
of molecular products and gives information about free
radical processes [4]. The intensity of chemilumines-
cence is measured in light quantum’s (pulse/s). The
volume of the sample measured was 0.2-0.5 ml. Time of
measurement was 3-5 minutes.
The chemiluminescence measuring of homogenates
of tissues, induced with hydrogen dioxide, was conduc-
ted on a quantometer with PEA-140. The measurement
was conducted in a temperature-controlled bottle at
370 C. The three parameters of luminescence kinetics
were analysed: intensity of maximal scintillation (Imax),
intensity of final value (Ifin) and light pulse sum for 4
minutes of measuring (Σ).
28 patients in the age from 12 to 71 years, in total of
them 8 men and 20 women were examined. The thyroid
gland cancer was detected for 19 patients, for 5 - nodal
macrofollicular goitre, for 4 - adenoma of thyroid gland.
62 ВОПРОСЫ АТОМНОЙ НАУКИ И ТЕХНИКИ. 2000, № 2.
Серия: Ядерно-физические исследования (36), c. 62-63.
The materials were taken from 3 bands: 2 cm3 of tumor
from the tumor centre, 2 cm3 of tissue on the boundary
of tumoral and normal tissue (paratumor) and similar
volume of tissue from the non-malignant band. The ho-
mogenates of tissues were prepared from 50 mg of tis-
sues examined with the help of mechanical and hand-op-
erated homogenises.
RESULTS
The measuring of the isotopic ratio 44Ca/48Ca in tis-
sues of thyroid gland has allowed one to suggest about
the transmembrane calcium circulation. The ratios were
in normal tissue -1, in paratumor - 1.76, in non-malig-
nant - 0.87, in malignant - 0.58. The error of measure-
ment was 10%. The presented data have shown that the
isotopic ratio 44Ca/48Ca in cancerous tissues is reduced
as compared to normal and paratumor tissues. Probably
here the change of calcium-transporting ability due to
transformation of normal tissues into cancerous tissues
takes place.
The higher content of the light calcium fraction in
paratumor tissue testifies to enhancing its circulation.
The disturbance of a membranous Са2+ transport modi-
fies endocellular control cascades, development of
membranous damages, which can be the factor of chan-
ging the free radical of peroxide oxidation of membrane
lipids.
It is known that the enhanced circulation of Са2+ is
conditioned by the activation of proteinkinase C [5] with
phosphorylation of some proteins and realization of long
term control stage. On the other hand the activators of a
proteinkinase C disturb interlinking with receptors of the
cell of tumor necrosis factor (TNFα) [6]. At the same
time the proteinkinase C stimulates production of
mRNA coding synthesis of TNFα. The tumor necrosis
factor is executive mediator of inflammation, which
causes high values of scintillation chemiluminescence in
paratumor. The superexpression of proteinkinase C
causes transformation of cells in vitro [6]. In our case
the nonreversible activation of proteinkinase C with ini-
tiation of cell fission processes is possible [7].
The table represents the data on kinetics of induced
chemiluminescence of tissue homogenates.
Form of tissues Imax Ifin Σ
Normal tissues 2060
± 128
1156
± 81
2328
± 113
Paratumor 6982
± 111
1877
± 97
5248
± 191
Non-malignant-
tissues
1231
± 91
739
± 39
1234
± 87
Cancerous
tissues
639
± 44
392
± 25
1093
± 66
The low intensity of chemiluminescence of malignant
tissues is already the proven fact. It is explained by high
concentration of inhibitors of free radical of peroxide
oxidation, which is determined by the high total antiox-
idative activity of tumor lipids of [8]. The chemilumin-
escence of paratumor tissue homogenates differ high
amplitude of maximal chemiluminescence scintillation
that evidences on high oxidizability of lipids in mem-
branes. The sum of chemiluminescence light pulses has
high values too. Apparently, the paratumor tissue can be
considered as a precursor of cancerous germ formation
and consequently of cancerous progression. The intens-
ity of free radical in this case is as inhibitor of cell pro-
liferation. But at the same time higher value Ifin evid-
ences that the cancerous progression is caused by accu-
mulation of bioantioxidants. The chemiluminescence
method is one of the most sensitive for studying the kin-
etics of free radical oxidation both in malignant and
non-malignant tumors, and in paratumor tissues. The
disequilibrium between anti- and proradical products,
apparently, is connected with damage of physicochemic-
al properties for membranous calcium transport.
Thus, the gamma activation analysis of isotopic ratio of
calcium and chemiluminescence methods of estimating free
radical oxidation intensity are high-performance research
techniques for studying the processes in cancerous and nor-
mal tissues of thyroid gland. The character of isotopic ratio
between calcium and free radical in tissues of thyroid gland
has a definite dependence for normal and malignant pro-
cesses. This process can appear to be interdependent cas-
cades in damage of endocellular structures and explains
some parties of control in metabolic processes of an organ-
ism.
REFERENCES
1. Е.S. Rom-Boguslavskaya et al. Radioimmunological
examinations in complex estimation of thyroid status
in the case of primary hypothyroidism // Med.
Radiol., 1990, No 2, p. 14-17 (in Russian).
2. О.М. Аstap`eva et al. Prognosis of effectiveness of
cancer thyroid gland treatment // Ukr. Radiol.
Zhourn., 1999, No 1, p. 14-15 (in Russian).
3. S.S. Yakovleva. Metabolism of Са in cells and interre-
lation with its exchange infringement // Usp. Sovr. Biol.
1990, v. 110, No 3(6), p. 363-377 (in Russian).
4. N.P. Dikiy et al. Physical analytical complex in
medicine. Nuclear-physical methods. Statistical
handling. Computer diagnostics. Preprint KFTI, 94-
13, Kharkov, 1994, 17 p. (in Russian).
5. D.L. Alkon, H. Rasmussen. A Spatial–Temporal
Model of Cell Activation // Science, 1988, v. 239,
No 4843, p. 998-1005.
6. M. Kronke, S. Schultze, P. Scheurich et al. Tumor
necrosis factor signal transduction // Cell.
Signalling. 1990, v. 2, p. 1-8.
7. M.A. Paltzev, A.A. Ivanov. Intercellular interaction,
M., Medicine, 1995, 224 p. (in Russian).
8. Е.А. Neyfakh. Superoxide dependent toxicity of
lipids from tumoral-carrier organs. Free radical
oxidizing of lipids in norm and in pathology. M.,
1976, p. 121-122 (in Russian).
63
National Science Center “Kharkov Institute of Physics and Technology”, Kharkov, Ukraine
A.A. Khizhnyak, N.V. Krasnoselsky
INTRODUCTION
METHODS
RESULTS
Form of tissues
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