Ozonizer with superimposed discharge for inactivation of microorganisms
The working characteristics of the sterilizer, which acts with use of ozone and ultrasonic cavitation, have been investigated. In this sterilizer the ozone is produced by ozone reactor with superposition of dielectric barrier discharge and surface discharge. The ozone concentration in sterilization...
Збережено в:
| Опубліковано в: : | Вопросы атомной науки и техники |
|---|---|
| Дата: | 2011 |
| Автори: | , , , |
| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
Національний науковий центр «Харківський фізико-технічний інститут» НАН України
2011
|
| Теми: | |
| Онлайн доступ: | https://nasplib.isofts.kiev.ua/handle/123456789/90953 |
| Теги: |
Додати тег
Немає тегів, Будьте першим, хто поставить тег для цього запису!
|
| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Ozonizer with superimposed discharge for inactivation of microorganisms / V.S. Taran, O.G. Chechelnizkij, V.V. Krasnyj, O.M. Shvets // Вопросы атомной науки и техники. — 2011. — № 1. — С. 161-163. — Бібліогр.: 5 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| id |
nasplib_isofts_kiev_ua-123456789-90953 |
|---|---|
| record_format |
dspace |
| spelling |
Taran, V.S. Chechelnizkij, O.G. Krasnyj, V.V. Shvets, O.M. 2016-01-06T12:01:26Z 2016-01-06T12:01:26Z 2011 Ozonizer with superimposed discharge for inactivation of microorganisms / V.S. Taran, O.G. Chechelnizkij, V.V. Krasnyj, O.M. Shvets // Вопросы атомной науки и техники. — 2011. — № 1. — С. 161-163. — Бібліогр.: 5 назв. — англ. 1562-6016 PACS: 52.77.-J https://nasplib.isofts.kiev.ua/handle/123456789/90953 The working characteristics of the sterilizer, which acts with use of ozone and ultrasonic cavitation, have been investigated. In this sterilizer the ozone is produced by ozone reactor with superposition of dielectric barrier discharge and surface discharge. The ozone concentration in sterilization bath was enhanced up to 10 mg/l owing to water temperature lowering to 15 °C with help of thermoelectric cooling module. The spectrometric researches of dielectric barrier discharge, surface discharge, and theirs combination have been accomplished. The experiments on bacteria sterilization in present sterilizer have shown that inactivation of Bacillus Cereus, E. coli, S. aurues takes 3…5 min, whereas the inactivation of spores requires 15 minutes. Досліджено робочі характеристики стерилізатора, у якому використовується спільна дія озону й ультразвукової кавітації . Озон виробляється в генераторі озону із суперпозицією діелектричного бар’єрного і поверхневого розрядів. Концентрація озону в стерилізаційній ванні – до 10 мг/л при температурі води 15 °C. Виконано спектрометричні дослідження діелектричного бар’єрного розряду, поверхневого розряду і їхньої комбінації. Проведені експерименти по знищенню бактерій показали, що інактивація Bacillus Cereus, E. coli, S.aurues займає 3…5 хв., а для знищення спор потрібно 15 хв. Исследованы рабочие характеристики стерилизатора, в котором используется совместное действие озона и ультразвуковой кавитации. Озон вырабатывается в генераторе озона с суперпозицией диэлектрического барьерного и поверхностного разрядов. Концентрация озона в стерилизационной ванне – до 10 мг/л при температуре воды 15 °C. Выполнены спектрометрические исследования диэлектрического барьерного разряда, поверхностного разряда и их комбинации. Проведенные эксперименты по уничтожению бактерий показали, что инактивация Bacillus Cereus, E. coli, S. aurues занимает 3...5 мин., а для уничтожения спор требуется 15 мин. The present work was supported by STCU project P381 en Національний науковий центр «Харківський фізико-технічний інститут» НАН України Вопросы атомной науки и техники Низкотемпературная плазма и плазменные технологии Ozonizer with superimposed discharge for inactivation of microorganisms Озонатор на основі сумісного розряду для інактивації мікроорганізмів Озонатор на основе совместного разряда для инактивации микроорганизмов Article published earlier |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| title |
Ozonizer with superimposed discharge for inactivation of microorganisms |
| spellingShingle |
Ozonizer with superimposed discharge for inactivation of microorganisms Taran, V.S. Chechelnizkij, O.G. Krasnyj, V.V. Shvets, O.M. Низкотемпературная плазма и плазменные технологии |
| title_short |
Ozonizer with superimposed discharge for inactivation of microorganisms |
| title_full |
Ozonizer with superimposed discharge for inactivation of microorganisms |
| title_fullStr |
Ozonizer with superimposed discharge for inactivation of microorganisms |
| title_full_unstemmed |
Ozonizer with superimposed discharge for inactivation of microorganisms |
| title_sort |
ozonizer with superimposed discharge for inactivation of microorganisms |
| author |
Taran, V.S. Chechelnizkij, O.G. Krasnyj, V.V. Shvets, O.M. |
| author_facet |
Taran, V.S. Chechelnizkij, O.G. Krasnyj, V.V. Shvets, O.M. |
| topic |
Низкотемпературная плазма и плазменные технологии |
| topic_facet |
Низкотемпературная плазма и плазменные технологии |
| publishDate |
2011 |
| language |
English |
| container_title |
Вопросы атомной науки и техники |
| publisher |
Національний науковий центр «Харківський фізико-технічний інститут» НАН України |
| format |
Article |
| title_alt |
Озонатор на основі сумісного розряду для інактивації мікроорганізмів Озонатор на основе совместного разряда для инактивации микроорганизмов |
| description |
The working characteristics of the sterilizer, which acts with use of ozone and ultrasonic cavitation, have been investigated. In this sterilizer the ozone is produced by ozone reactor with superposition of dielectric barrier discharge and surface discharge. The ozone concentration in sterilization bath was enhanced up to 10 mg/l owing to water temperature lowering to 15 °C with help of thermoelectric cooling module. The spectrometric researches of dielectric barrier discharge, surface discharge, and theirs combination have been accomplished. The experiments on bacteria sterilization in present sterilizer have shown that inactivation of Bacillus Cereus, E. coli, S. aurues takes 3…5 min, whereas the inactivation of spores requires 15 minutes.
Досліджено робочі характеристики стерилізатора, у якому використовується спільна дія озону й ультразвукової кавітації . Озон виробляється в генераторі озону із суперпозицією діелектричного бар’єрного і поверхневого розрядів. Концентрація озону в стерилізаційній ванні – до 10 мг/л при температурі води 15 °C. Виконано спектрометричні дослідження діелектричного бар’єрного розряду, поверхневого розряду і їхньої комбінації. Проведені експерименти по знищенню бактерій показали, що інактивація Bacillus Cereus, E. coli, S.aurues займає 3…5 хв., а для знищення спор потрібно 15 хв.
Исследованы рабочие характеристики стерилизатора, в котором используется совместное действие озона и ультразвуковой кавитации. Озон вырабатывается в генераторе озона с суперпозицией диэлектрического барьерного и поверхностного разрядов. Концентрация озона в стерилизационной ванне – до 10 мг/л при температуре воды 15 °C. Выполнены спектрометрические исследования диэлектрического барьерного разряда, поверхностного разряда и их комбинации. Проведенные эксперименты по уничтожению бактерий показали, что инактивация Bacillus Cereus, E. coli, S. aurues занимает 3...5 мин., а для уничтожения спор требуется 15 мин.
|
| issn |
1562-6016 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/90953 |
| citation_txt |
Ozonizer with superimposed discharge for inactivation of microorganisms / V.S. Taran, O.G. Chechelnizkij, V.V. Krasnyj, O.M. Shvets // Вопросы атомной науки и техники. — 2011. — № 1. — С. 161-163. — Бібліогр.: 5 назв. — англ. |
| work_keys_str_mv |
AT taranvs ozonizerwithsuperimposeddischargeforinactivationofmicroorganisms AT chechelnizkijog ozonizerwithsuperimposeddischargeforinactivationofmicroorganisms AT krasnyjvv ozonizerwithsuperimposeddischargeforinactivationofmicroorganisms AT shvetsom ozonizerwithsuperimposeddischargeforinactivationofmicroorganisms AT taranvs ozonatornaosnovísumísnogorozrâdudlâínaktivacíímíkroorganízmív AT chechelnizkijog ozonatornaosnovísumísnogorozrâdudlâínaktivacíímíkroorganízmív AT krasnyjvv ozonatornaosnovísumísnogorozrâdudlâínaktivacíímíkroorganízmív AT shvetsom ozonatornaosnovísumísnogorozrâdudlâínaktivacíímíkroorganízmív AT taranvs ozonatornaosnovesovmestnogorazrâdadlâinaktivaciimikroorganizmov AT chechelnizkijog ozonatornaosnovesovmestnogorazrâdadlâinaktivaciimikroorganizmov AT krasnyjvv ozonatornaosnovesovmestnogorazrâdadlâinaktivaciimikroorganizmov AT shvetsom ozonatornaosnovesovmestnogorazrâdadlâinaktivaciimikroorganizmov |
| first_indexed |
2025-11-26T15:11:37Z |
| last_indexed |
2025-11-26T15:11:37Z |
| _version_ |
1850625866252419072 |
| fulltext |
PROBLEMS OF ATOMIC SCIENCE AND TECHNOLOGY. 2011. 1. 161
Series: Plasma Physics (17), p. 161-163.
OZONIZER WITH SUPERIMPOSED DISCHARGE FOR INACTIVATION
OF MICROORGANISMS
V.S. Taran, O.G. Chechelnizkij, V.V. Krasnyj, O.M. Shvets
Institute of Plasma Physics, NSC “Kharkov Institute of Physics and Technology”, Kharkov, Ukraine
E-mail: vtaran@ipp.kharkov.ua
The working characteristics of the sterilizer, which acts with use of ozone and ultrasonic cavitation, have been
investigated. In this sterilizer the ozone is produced by ozone reactor with superposition of dielectric barrier discharge
and surface discharge. The ozone concentration in sterilization bath was enhanced up to 10 mg/l owing to water
temperature lowering to 15 ° with help of thermoelectric cooling module. The spectrometric researches of dielectric
barrier discharge, surface discharge, and theirs combination have been accomplished. The experiments on bacteria
sterilization in present sterilizer have shown that inactivation of Bacillus Cereus, E. coli, S. aurues takes 3…5 min,
whereas the inactivation of spores requires 15 minutes.
PACS: 52.77.-J
1. INTRODUCTION
At the present time the problem of low temperature
sterilization is very actual. The ozone technologies with the
use of the barrier glow discharge are one of the promising
methods and a good alternative for the above-mentioned
methods [1,2,3,4]. The expansion of the spheres of the
application of ozone initiates new studies directed toward
improvements in the characteristics of reactors for obtaining
ozone. Different methods, connected with the pre-ionization in
the main discharge, so-called dual discharges, also attract an
attention [5]. The ozoniser with superposition of dielectric
barrier discharge (DBD) and surface discharge seems as one
of the most perspective.
Additional possibilities appear with generation of
ultrasonic cavitation in ozonized media. In present work
we investigated the low-temperature ozone sterilizer with
ultrasonic cavitation.
2. EXPERIMENTAL APPARATUS
AND METHOD
The ozone reactor with superposition of double barrier
and surface discharges was used for ozone producing. The
scheme of superposed discharge under atmospheric
pressure is shown in Fig. 1. Dielectric barrier discharge
was generated between two flat metallic electrodes
(180×100 mm), which were coated with glass-enamel
dielectric material, having a dielectric constant equal to 7
and a thickness of 0.2 mm. The high-voltage pulses were
applied to these electrodes. The first of the electrodes for
the surface discharge was composed of still strips of
2 mm width and was placed on the top glass-enamel
surface. The second one was arranged on the bottom
surface. The dielectric barrier discharge and the surface
discharge have been excited by ac voltages of 8 and 4 kV
respectively. The phase between applied voltages can be
varied from 0 to 180°. Discharges were exited in dry air
which after airflow controller flows through a 2.5 mm gap
between electrodes. Parameters of these discharges were
measured using ozone monitor, oscillograph, Rogowski
coil and spectrometer. The data from these devices were
processed by PC system with multifunctional plate input-
output type L-Card L-783 (3MHz, 32 channels). The
information system modules were based on C++Builder-6.
3. RESULTS AND DISCUSSION
3.1. OZONE REACTOR CHARACTERISTICS
The ozone concentration at reactor output was
measured for every of three possible regimes of its
functioning: 1) surface discharge; 2) dielectric barrier
discharge, and 3) the superposition of these discharges.
The results presented in Fig. 2 shows that breakdown
~
Air
flow
Glass-
enamel
~
Fig. 1. Ozone reactor with superposition of barrier
and surface discharges
Metallic strip
electrode
Fig. 2. Ozone generation characteristics of
surface discharge, DBD and superimposed
discharge
2 4 6 8 10
0
2
4
8
10
Surface
DBD
Superposition
O
zo
ne
C
on
ce
nt
ra
tio
n,
m
g/
l
Voltage, kV
mailto:vtaran@ipp.kharkov.ua
162
voltage required for the dielectric barrier discharge
ignition is lower in combined discharge than in single
barrier discharge (6 and 8 kV respectively). In addition,
the ozone concentration is approximately 1.5 times
greater in combined discharge than in DBD discharge.
The number of streamers, estimated by Rogowski coil,
in superposed discharges is greater as compared to the
singles DBD or surface ones. The maximal number of
streamers is observed under the maximum phase shift, in
180°, between of voltages applied to the electrodes of
surface and DBD discharges.
Spectroscopic measurements, which were carried out
on spectrometer SL40-2-3648USB, have shown the
changes in concentration of nitrogen in dependence on
parameters of discharges that was exited in pure air at one
atmosphere and room temperature. Fig. 3 shows the
emission spectra obtained over the wavelength range of
200…800 nm from surface and DBD combined
discharges under phase shift in 180° between of voltages
applied to surface and DBD discharges. The spectral
lines intensity of nitrogen is maximal at phase shift in
180° that corresponds to the maximum electric field
strength in the discharge gap of the reactor and connected
with increasing the discharge power. The lines intensity in
combined discharge under 180° phase shift between the
applied voltages is substantially larger than in all others
cases. The growth in ozone concentration is a
consequence of the major intensity of combined discharge
as compared with surface and DBD discharges.
3.2. OPERATING MODES OF THE STERILIZER
The functional diagram of sterilizer is shown in Fig. 4.
From reactor an ozone-air mixture goes to the ultrasonic
bath filled with distilled water. The ultrasonic source
generates ultrasonic cavitations that enable the penetration
of ozonized water into cavitations that enables the
penetration of ozonized water into cavities and gaps of
sterilized instruments and performance of theirs
presterilization treatment. Water is cooling by
thermoelectric cooler. The work of ozone reactor,
ultrasonic source and thermoelectric cooler is headed by
microprocessor. It is well known that ozone solubility
strongly depends on water temperature. The considerable
enhancement of ozone concentration and effectiveness of
the sterilization may be provided by periodic switching and
turning off of the ultrasonic source. When the water
temperature is decreased to the lower designated limit, the
ultrasonic source starts to operate, providing a cavitations
that, in turn, causes water heating. When the water
temperature reaches the upper of these designated limits,
the ultrasonic source stops operating and water temperature
drops. Fig. 5 shows the operating mode of sterilizer, when
the upper setting temperature have been chosen at 150C,
and lower level of temperature was 140 C. The initial water
and air temperatures was 19 0C, the rate of air flow through
the reactor was maintained near 0.2 l/min. The reactor
yielded at these conditions the ozone concentration of
36 mg/l in air and near 10 mg/l in water.
Fig. 4. Block diagram of sterilizer with a cooling module
3.3. BACTERICIDAL INFLUENCE OF STERILIZER
ON THE TEST-CULTURES OF MICROORGANISM
Experiments on the bacteria inactivation were carried
out in ozonized distilled water. The following test-
cultures of microorganism have been studied:
1. E. coli 055 K59 3912/41, the Staphylococci aureus
ATCC 25923, Pseudomonas aeruginoza 27/99 seeded
on meat-peptone broth (MPB), as well as Cl.Oedematiens
198 and B. cereus 8035 seeded on this medium.
2. Test-cultures seeded on the agar endo, 6, 5 % salt agar
and meat-peptone agar (MPA) with 1% glucose. In the
smears it is established 70…80% of spore culture
Cl. Oedematiens 198 and B. cereus 8035. On the endo
agar it is seen the increase of the colonies of dark-cherry
color with the metallic luster. On the salt agar it is seen
the growing of colonies of gray-white color. On MPA the
colonies of blue-green color increased.
Fig. 3. Emission intensity for lines of N2 .in
combined discharges under voltage of 8 kV and
a phase shift in 180° between of voltages applied to
the electrodes of surface and DBD discharges
COOLER
OZON
PUMP
MIXER
ULTRASOUND
BATH
WATER
Time, min
O
zo
ne
c
on
ce
nt
ra
tio
n,
m
g/
l
W
at
er
te
m
pe
ra
tu
re
,0 C
20 40 60 80 100
0
2
4
6
8
10
12
0 120
0
5
10
15
20
Ozone concentration
Water temperature
Fig. 5. Time dependence of ozone concentration
under maintaining of water temperature between
14 and 15 0C
200
400
800
0
Wavelength (nm)
300 400 500 600
600
Em
is
si
on
In
te
ns
ity
N
2
35
7.
69
n
m
N
2
33
7.
13
n
m
Combined discharge.
8 kV, 1800 phase shift
163
3. The isolated colonies were seeded on MPB and MPA.
4. The plasma-coagulation reaction is positive with the
staphylococcal culture.
5. Daily cultures E. coli, Staphylococcus aureus,
Pseudomonas aeruginoza and seven-day spore culture
Cl. Oedematiens were placed into sterilization camera and
processed by ozone and ultrasound over the time of 90
minutes. The control samples have been taken from the
sterilization water bath after 1, 3, 5, 10, 20, 30, 60, 90
minutes for a microbiological analysis. During these time
intervals the ozone concentration increased from 0 to the
values indicated in second column of the Table.
Time-kill assay for some bacteries
Test - culture
Conc. 3
in water
From
0 mg/l to:
Water
temper.
from
0° to:
Time-kill
assay
(min)
E. coli 1.8 19 2
Staphylococcus
aureus
1.8 19 2
Pseudomonas
aeruginoza
6 17.5 5
Cl.Oedematiens
198
8.5 16.25 10
B. cereus 8035 10 15 15
It have been found that the complete inactivation of
E. coli and Staphylococcus aureus is realized under small
ozone concentration and demands no more than 2 minutes.
Meanwhile, the most persistent micro organisms – spores
B. cereus 8035, takes 15 minutes during which the
ozone concentration in water rises from 0 to 10 mg/l, and
the temperature reduces from 19 to 15 0C.
CONCLUSIONS
The low-temperature sterilizers used for disinfection and
sterilization in water has been developed and investigated. The
combined action of ozone and ultrasonic cavitations in
aqueous medium makes it possible to achieve effective
cleaning of the articles of complex form with internal cavities
and possibilities of the inactivation of microorganisms on tools
of complex configuration.
Physical principles grounded in the sterilization process
allowed applying such devices in industry and for everyday
purposes, for instance: medical tools, biotechnology,
microelectronics, food industry, automobile construction,
household devices, etc.
The output ozone concentration from the reactor of
30 mg/l provided the ozone concentration in a sterilization
bath (2.5 and 0.5 liters) of order 10 mg/l. This concentration
was obtained due to a specially designed water cooling
module.
The ozonizer with superposition of double barrier and
surface discharges has been proposed as an alternative to
double discharge-type generators. Two high-voltage pulsed
power supplies of 10 and 20 W have been used in such an
ozonizer equipped with three parallel electrodes (central,
surface, and outer one).
The phase between applied voltages was varied from 0° to
180°. An increase of the phase displacement in the main
discharge leads to an increase in the output concentration of
ozone and nitric components in the consequence of an increase
of the field strength in the reactor
The breakdown voltage in the superimposed discharge
occurs with the smaller applied voltage as compared to DBD
(6 and 8 kV, respectively).
The output ozone concentration increases with increasing
the discharge power. An increase of the main discharge gap
from 1.5 to 2.5 mm allows increasing the output ozone
concentration.
ACKNOWLEDGEMENTS
The present work was supported by STCU project P381
REFERENCES
1. N.N. Morgan // International Journal of Physical
Sciences. 2009, v. 4, N 13, p. 885-892.
2. G. Fridman, A. Gutsol et al. // Applied Plasma Medicine.
Plasma Processes and Polymers. 2008, v. 5(6), p. 503.
3. M. Laroussi, D.A. Mendis and M Rosenberg // New
Journal of Physics. 2003, v. 5, . 41.1– 41.10.
4. A. Chirokov, A. Gutsol and A. Fridman. Atmospheric
pressure plasma of dielectric barrier discharges // Pure
Appl. Chem. 2005, v. 77(2), p. 487–495.
5. Hee-Sung Ahn, Nobuya Hayashi et al. Ozone Generation
Characteristics of Superimposed Discharge with Surface
and DC Discharge //Rep. Fac. Sci. Engrg./ Saga Univ,
2001, p. 25-32.
Article received 23.11.10
. , . , . , .
,
.
. – 10
15 ° . ,
. ,
Bacillus Cereus, E. coli, S. aurues 3…5 , 15 .
. , . , . , .
,
.
. – 10 15 ° .
,
. , Bacillus Cereus, E. coli,
S. urues 3…5 ., 15 .
|