The dynamics of element content in patients with lung cancer during radiotherapy
Determination of the element contents in the biological samples (blood, serum of blood, hair) of patients with the
 diagnosis of lung cancer III degree is carried out during beam therapy. The method of PIXE excited by protons on
 the accelerator with energy 3 MeV has been used. Durin...
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| Опубліковано в: : | Вопросы атомной науки и техники |
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| Дата: | 2009 |
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Національний науковий центр «Харківський фізико-технічний інститут» НАН України
2009
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| Цитувати: | The dynamics of element content in patients with lung cancer during radiotherapy / N.P. Dikiy, Yu.V. Lyashko, E.P. Medvedeva, V.I. Borovlev, V.D. Zabolotny, D.V. Medvedev, N.I. Pilipenko, V.P. Starenky, E.N. Suhina // Вопросы атомной науки и техники. — 2009. — № 5. — С. 50-53. — Бібліогр.: 10 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1860148711057784832 |
|---|---|
| author | Dikiy, N.P. Lyashko, Yu.V. Medvedeva, E.P. Borovlev, V.I. Zabolotny, V.D. Medvedev, D.V. Pilipenko, N.I. Starenky, V.P. Suhina, E.N. |
| author_facet | Dikiy, N.P. Lyashko, Yu.V. Medvedeva, E.P. Borovlev, V.I. Zabolotny, V.D. Medvedev, D.V. Pilipenko, N.I. Starenky, V.P. Suhina, E.N. |
| citation_txt | The dynamics of element content in patients with lung cancer during radiotherapy / N.P. Dikiy, Yu.V. Lyashko, E.P. Medvedeva, V.I. Borovlev, V.D. Zabolotny, D.V. Medvedev, N.I. Pilipenko, V.P. Starenky, E.N. Suhina // Вопросы атомной науки и техники. — 2009. — № 5. — С. 50-53. — Бібліогр.: 10 назв. — англ. |
| collection | DSpace DC |
| container_title | Вопросы атомной науки и техники |
| description | Determination of the element contents in the biological samples (blood, serum of blood, hair) of patients with the
diagnosis of lung cancer III degree is carried out during beam therapy. The method of PIXE excited by protons on
the accelerator with energy 3 MeV has been used. During radiotherapy the difference in the element contents in hair
of patients has not been detected. Trustworthy reduction of Fe, Cu, Mn, Ca, Sr, Rb etc is marked during treatment.
The obtained results can be used as the additional test at carrying out adequate treatment in radiotherapy.
Проведено визначення вмiсту мiкроелементiв в бiологiчних зразках (кров, сироватка кровi, волосся)
пацiєнтiв з дiагнозом раку легенi III ступеня в процесi променевої терапiї. Використовувався метод характеристичного рентгенiвського випромiнювання, яке збуджувалось протонами прискорювача з енергiєю 3 МеВ. Протягом радiотерапiї вiдмiнностей у вмiстi елементiв у волоссях пацiєнтiв виявлено не
було. У процесi лiкування вiрогiдно виявлене зменшення Fe, Cu, Mn, Ca, Sr, Rb i т.д. в кровi i сироватцi
кровi. Отриманi результати можуть бути використанi як додатковий тест при проведеннi адекватної
терапiї в курсi променевого лiкування.
Проведено определение содержания микроэлементов в биологических образцах (кровь, сыворотка крови, волосы) пациентов с диагнозом рака легкого III степени в процессе лучевой терапии. Использован
метод характеристического рентгеновского излучения, возбуждаемого протонами на ускорителе с энергией 3 МэВ. В течение радиотерапии отличий в содержании элементов в волосах пациентов обнаружено
не было. В процессе лечения достоверно обнаружено уменьшение Fe, Cu, Mn, Ca, Sr, Rb и т.д. в крови и
сыворотке крови. Полученные результаты могут быть использованы в качестве дополнительного теста
при проведении адекватной терапии в курсе лучевого лечения.
|
| first_indexed | 2025-12-07T17:51:26Z |
| format | Article |
| fulltext |
THE DYNAMICS OF ELEMENT CONTENT IN PATIENTS
WITH LUNG CANCER DURING RADIOTHERAPY
N.P. Dikiy1∗, Yu.V. Lyashko1, E.P. Medvedeva1,
V.I. Borovlev1, V.D. Zabolotny1, D.V. Medvedev1,
N.I. Pilipenko2, V.P. Starenky2, E.N. Suhina2
1National Science Center ”Kharkov Institute of Physics and Technology”, 61108, Kharkov, Ukraine
2Grigoriev Institute for Radiology, 61024, Kharkov, Ukraine
(Received June 6, 2009)
Determination of the element contents in the biological samples (blood, serum of blood, hair) of patients with the
diagnosis of lung cancer III degree is carried out during beam therapy. The method of PIXE excited by protons on
the accelerator with energy 3 MeV has been used. During radiotherapy the difference in the element contents in hair
of patients has not been detected. Trustworthy reduction of Fe, Cu, Mn, Ca, Sr, Rb etc is marked during treatment.
The obtained results can be used as the additional test at carrying out adequate treatment in radiotherapy.
PACS: 29.30.Kw
1. INTRODUCTION
The lung cancer is the most widespread oncological
disease, in particular, among males, and its incidence
is above 30% [1]. The risk factors of this disease may
be related to smoking, occupational activity, environ-
mental contamination, chronic bronchitis and pneu-
monia. The influence of many risk factors on the
tumor development and different mechanisms of mi-
croelement (ME) transport into the malignant and
unaffected tissues is one of primary problems in the
study of this disease. The ME content in normal or-
gans and tissues for the most part will depend on the
age, sex, mode of nutrition, geographic-and-climatic
conditions and genetic factor.
Biological role of such elements as Fe, Cu, Zn,
Mn, Se, Rb, Br, I, Ca etc in different diseases is in-
tensively studied [2,3]. Almost all these elements are
basic components of ferments and biologically active
systems. All metals, except I, F and Se play impor-
tant role as cofactors in enzymes. Fe in the form of
heme is contained in the composition of hemoglobin,
myoglobin, cytochrome and dehydrogenase. Cu is
contained in a number of ferments (tyrozine, amine
oxidase, cytochrome-c-oxidase, transferase etc) and
the role of Cu in the vitamin B12 is known. Zinc is
in the composition of carboxypeptide, aminopeptide
etc [4]. Se, as an element-antioxidant, was detected
in many tumor tissues [5]. Different concentrations of
Mn are found in earlier proliferative phases. There is
available information about the function of someone
else essential ME in the case of oncogenesis.
The concentration of essential ME can undergo
significant changes in the course of the radiotherapy
and chemotherapy. In the latter case it is very impor-
tant to know how these ME can be used as biological
additives for cancer carriers in the course of diagnosis
and prognosis. The main goal of this work is to study
the element contents of the blood serum and hairs of
lung cancer patients in the course of radiotherapy.
2. MATERIALS AND METHODS
The method of characteristic X-ray (PIXE) excited
by protons was used to determine the ME content
in the biological samples (blood, blood serum, hair)
being investigated [6].
The main principle of the method applied is the
X-ray excitation in the samples under the action of
accelerator protons. The PIXE spectrum from the
target permits to obtain qualitative information on
the ME content in the samples.
The measurements were carried at accelerator
PG-5 with energy 3 MeV of NSC KIPT. The 2.5 MeV
proton beam collimated to 3 mm was impinging on
the target at an angle of 45o. The intensity of the
beam impinging on the Faraday cap was measured
by the current integrator. PIXE from the target,
after passing through the 10 µm window of thin
aluminium-metallized Mylar, was measured by means
of the Si(Li)-detector with a resolution of 280 eV on
the line of 6.4 keV and 30 mm2 area placed in the
vacuum system. The absolute limit of detecting was
achieved 10−12 g, and relative - 0,1 µg/g. The ab-
solute values of ME concentrations were determined
by the standard sample-preparation method and by
introducing the internal standard (yttrium).
Mathematical spectrum treatment was performed
by the SLED program. The papers presents the re-
∗Corresponding author E-mail address: ndikiy@kipt.kharkov.ua
50 PROBLEMS OF ATOMIC SCIENCE AND TECHNOLOGY, 2009, N5.
Series: Nuclear Physics Investigations (52), p.50-53.
sults of survey for 32 lung cancer patients (stage III)
undergoing the course of radiotherapy by the radi-
cal program in the department of distance radio- and
complex therapy of the Kharkov Research Institute
for Medical Radiology. In the group of examined pa-
tients were males at the age from 45 to 65 years. For
71% of these patients the diagnosis was confirmed
morphologically and corresponded to the following
diseases: adenocarcinoma of a different degree of dif-
ferentiation 6 (26%), epidermoid carcinoma 17 (74%),
in the rest 9 cases the cancer was diagnosed without
histological structure specification.
The patients were irradiatied at the linear elec-
tron accelerator LUEV-15M1 under conditions of
bremsstrahlung with a limiting quantum energy of
15 MeV. The treatment was carried out by the two-
pole method - anterior and posterior fields on the
lesion side or with the capture of an opposite root of
lung. The total focal dose was varying from 55 to
65 Gy depending on the morphological structure and
the degree of tumor differentiation and was reached
during 5-7 weeks by the splitting method.
The control group comprised 20 donors. Inves-
tigations of blood serum- and hair samples were re-
peated three times: before the treatment, in the mid-
dle and after the termination of the first step of the
radical irradiation program, respectively.
Liquid blood, blood serum samples were dried in
the drying chamber at a temperature of 40◦ C until
the sample weight was unchangeable during 3 days,
then its were minced in the agate mortar and were
applied by thin layers (1...20 mg/cm2) onto the sub-
strate of spectroscopically pure graphite with a car-
rier of 50 mg/cm2 thickness. In one of samples added
were the reference elements, for example, yttrium and
elements-additives (from 3 to 5) of a given concentra-
tion minced in the agate mortar. The hair samples
were prepared according to the IAEA recommenda-
tions [7]. At the front of the detector an aluminium
filter of 15µm thickness was placed. The proton dose
was 3 µC and the intensity did not exceed 15 nA/cm2.
The detection limit was 1 µg/g of dried mass.
3. RESULTS AND DISCUSSION
The determination of the ME content in the blood
serum and hairs was carried out for each of samples
in the group of patients and donors. In Fig.1 shown
is the original spectrum of the element content in the
hairs of patients with lung cancer in stage III (pa-
tient P.) before the treatment. There were not ob-
served appreciable changes in the investigated sam-
ples in comparison with the norm. In the course of
radiotherapy there were not observed any significant
distinctions in the content of elements in the patient
hairs. However, during the investigation the Pb con-
centration in the hairs of all patients was 3...10 µg/g
that exceeded the mean Pb concentration relative to
the control group. The samples of some patients con-
tained uncertain increased selenium amount.
Fig.1. Spectrum of X-ray radiation from the blood serum of patients with lung cancer (patient O)
According to the data, obtained before the treat-
ment, the concentration of such elements as man-
ganese, strontium, rubidium etc in the blood serum
of patients is higher that of donors. The dynamics
of investigations on the elemental composition in the
blood serum during the course of radiotherapy has
demonstrated a certain decrease of almost all deter-
mined elements as compared to the content of these
51
elements before the treatment. Fig.2a and Fig.2b
show that such elements as iron, copper, calcium,
strontium undergo the most significant changes. It
is common knowledge that iron is a metal of a vari-
able valence participating in the process of free rad-
ical peroxidation (FRPO) in the stage of hydrogen
peroxide decomposition with formation of an active
radical leading to the chain branching [8,9].
Calcium ions are the FRPO regulators capable to
replace iron and to transform it in the active form.
Fig.2a. Content of elements in the blood of patients
with lung cancer depending on the treatment period
Fig.2.b. Content of elements in the blood of patients
with lung cancer depending on the treatment period
he decrease in the concentration of manganese and
copper is obviously related with the reducing activity
of such ferments as superoxide dismutase and cerulo-
plasmin that evidences on the decrease of the reserve
in this system and on the probability of inclusion of
other regulatory factors.
The presence of bromide in the blood serum and
character of its changing in the course of radiotherapy
may be related with the iatrogenic effect of patient
treatment.
It should pay attention to the behavior of such
an element as zinc, which in the dynamics of inves-
tigations almost does not undergo any changes. It is
in good agreement with the literature data confirm-
ing that the zinc concentration in the blood serum
is one of the most stable characteristics [10]. Zinc is
the element - FRPO inhibitor stabilizing the perme-
ability of cellular and intercellular membranes. This
element comes into competitive interrelations with
calcium and realizes its antagonism at a level of fer-
ments. So, by analyzing the results of investigation
on the elemental composition of the blood serum of
patients with lung cancer, it could be noted that some
of revealed changes reflect adequately the peculiari-
ties of organism reaction on the radiotherapy action.
The performed correlation analysis between the ele-
ment content values in the patient blood depending
on the treatment period did not revealed significant
changes, although the certain simplification of cor-
relation bonds took place. It has been established
that there are strong correlations between the ele-
ments determined before the treatment and these de-
termined in the middle of course of treatment (cor-
relation coefficient CC=0.88). The correlation bonds
were also significant (CC=0.52)) for the element con-
tents in the middle and in the termination of course
of treatment. Weakening of the correlation structure
(CC=0.44) is observed for the element content after
the termination of course of treatment with the same
value as in the beginning of the course of therapy.
The loss of significant bonds between zinc and boron
(CC=0.45), and, also, between rubidium and zinc
(CC=0.31) was observed. In conclusion, it should
be noted that the use of the multiple-element high-
sensitive PIXE method for determining the element
content in samples of patients with lung cancer (stage
III) is of a particular interest for the study of the
dynamics of changes in the organism’s microelement
status. The results obtained can be used when the ad-
equate corrective therapy is assigned in the process of
radiation exposure including Ca-antagonists, copper-
containing drugs and antioxidants.
4. CONCLUSIONS
1. The nuclear method PIXE was used for deter-
mining the ME content in the hair, blood and blood
serum of patients with lung cancer (stage III) during
the radical course of radiotherapy. Such elements as
Fe, Mn, Cu, Zn, Ca, Br, Sr, Rb were determined.
2. In the course of radiotherapy any significant
distinctions in the element content in the patient
hairs relative to the norm were not observed.
3. In the blood of patients with lung cancer be-
fore the radiotherapy application the concentration
of such elements as manganese, calcium, strontium,
rubidium was reliably higher than that of the donor.
The radiotherapy effect resulted in the significant de-
crease of all the elements being investigated.
4. The obtained results on the ME content in the
radiotherapy dynamics can be used for the course of
corrective therapy.
52
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ДИНАМИКА СОДЕРЖАНИЯ ЭЛЕМЕНТОВ У БОЛЬНЫХ РАКОМ ЛЕГКОГО В
ПРОЦЕССЕ ЛУЧЕВОЙ ТЕРАПИИ
Н.П. Дикий, Ю.В. Ляшко, Е.П. Медведева, В.И. Боровлев, В.Д. Заболотный,
Д.В. Медведев, Н.И. Пилипенко, В.П. Старенький, Е.Н. Сухина
Проведено определение содержания микроэлементов в биологических образцах (кровь, сыворотка кро-
ви, волосы) пациентов с диагнозом рака легкого III степени в процессе лучевой терапии. Использован
метод характеристического рентгеновского излучения, возбуждаемого протонами на ускорителе с энер-
гией 3 МэВ. В течение радиотерапии отличий в содержании элементов в волосах пациентов обнаружено
не было. В процессе лечения достоверно обнаружено уменьшение Fe, Cu, Mn, Ca, Sr, Rb и т.д. в крови и
сыворотке крови. Полученные результаты могут быть использованы в качестве дополнительного теста
при проведении адекватной терапии в курсе лучевого лечения.
ДИНАМIКА ВМIСТУ ЕЛЕМЕНТIВ У ХВОРИХ РАКОМ ЛЕГЕНI В ПРОЦЕСI
ПРОМЕНЕВОЇ ТЕРАПIЇ
М.П. Дикий, Ю.В. Ляшко, О.П. Медведєва, В.I. Боровльов, В.Д. Заболотний,
Д.В. Медведєв, М.I. Пилипенко, В.П. Старенький, О.М. Сухiна
Проведено визначення вмiсту мiкроелементiв в бiологiчних зразках (кров, сироватка кровi, волосся)
пацiєнтiв з дiагнозом раку легенi III ступеня в процесi променевої терапiї. Використовувався метод ха-
рактеристичного рентгенiвського випромiнювання, яке збуджувалось протонами прискорювача з енер-
гiєю 3 МеВ. Протягом радiотерапiї вiдмiнностей у вмiстi елементiв у волоссях пацiєнтiв виявлено не
було. У процесi лiкування вiрогiдно виявлене зменшення Fe, Cu, Mn, Ca, Sr, Rb i т.д. в кровi i сироватцi
кровi. Отриманi результати можуть бути використанi як додатковий тест при проведеннi адекватної
терапiї в курсi променевого лiкування.
53
|
| id | nasplib_isofts_kiev_ua-123456789-96428 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1562-6016 |
| language | English |
| last_indexed | 2025-12-07T17:51:26Z |
| publishDate | 2009 |
| publisher | Національний науковий центр «Харківський фізико-технічний інститут» НАН України |
| record_format | dspace |
| spelling | Dikiy, N.P. Lyashko, Yu.V. Medvedeva, E.P. Borovlev, V.I. Zabolotny, V.D. Medvedev, D.V. Pilipenko, N.I. Starenky, V.P. Suhina, E.N. 2016-03-16T20:00:15Z 2016-03-16T20:00:15Z 2009 The dynamics of element content in patients with lung cancer during radiotherapy / N.P. Dikiy, Yu.V. Lyashko, E.P. Medvedeva, V.I. Borovlev, V.D. Zabolotny, D.V. Medvedev, N.I. Pilipenko, V.P. Starenky, E.N. Suhina // Вопросы атомной науки и техники. — 2009. — № 5. — С. 50-53. — Бібліогр.: 10 назв. — англ. 1562-6016 PACS: 29.30.Kw https://nasplib.isofts.kiev.ua/handle/123456789/96428 Determination of the element contents in the biological samples (blood, serum of blood, hair) of patients with the
 diagnosis of lung cancer III degree is carried out during beam therapy. The method of PIXE excited by protons on
 the accelerator with energy 3 MeV has been used. During radiotherapy the difference in the element contents in hair
 of patients has not been detected. Trustworthy reduction of Fe, Cu, Mn, Ca, Sr, Rb etc is marked during treatment.
 The obtained results can be used as the additional test at carrying out adequate treatment in radiotherapy. Проведено визначення вмiсту мiкроелементiв в бiологiчних зразках (кров, сироватка кровi, волосся)
 пацiєнтiв з дiагнозом раку легенi III ступеня в процесi променевої терапiї. Використовувався метод характеристичного рентгенiвського випромiнювання, яке збуджувалось протонами прискорювача з енергiєю 3 МеВ. Протягом радiотерапiї вiдмiнностей у вмiстi елементiв у волоссях пацiєнтiв виявлено не
 було. У процесi лiкування вiрогiдно виявлене зменшення Fe, Cu, Mn, Ca, Sr, Rb i т.д. в кровi i сироватцi
 кровi. Отриманi результати можуть бути використанi як додатковий тест при проведеннi адекватної
 терапiї в курсi променевого лiкування. Проведено определение содержания микроэлементов в биологических образцах (кровь, сыворотка крови, волосы) пациентов с диагнозом рака легкого III степени в процессе лучевой терапии. Использован
 метод характеристического рентгеновского излучения, возбуждаемого протонами на ускорителе с энергией 3 МэВ. В течение радиотерапии отличий в содержании элементов в волосах пациентов обнаружено
 не было. В процессе лечения достоверно обнаружено уменьшение Fe, Cu, Mn, Ca, Sr, Rb и т.д. в крови и
 сыворотке крови. Полученные результаты могут быть использованы в качестве дополнительного теста
 при проведении адекватной терапии в курсе лучевого лечения. en Національний науковий центр «Харківський фізико-технічний інститут» НАН України Вопросы атомной науки и техники Ядернo-физические методы и обработка данных The dynamics of element content in patients with lung cancer during radiotherapy Динамiка вмiсту елементiв у хворих раком легенi в процесi променевої терапiї Динамика содержания элементов у больных раком легкого в процессе лучевой терапии Article published earlier |
| spellingShingle | The dynamics of element content in patients with lung cancer during radiotherapy Dikiy, N.P. Lyashko, Yu.V. Medvedeva, E.P. Borovlev, V.I. Zabolotny, V.D. Medvedev, D.V. Pilipenko, N.I. Starenky, V.P. Suhina, E.N. Ядернo-физические методы и обработка данных |
| title | The dynamics of element content in patients with lung cancer during radiotherapy |
| title_alt | Динамiка вмiсту елементiв у хворих раком легенi в процесi променевої терапiї Динамика содержания элементов у больных раком легкого в процессе лучевой терапии |
| title_full | The dynamics of element content in patients with lung cancer during radiotherapy |
| title_fullStr | The dynamics of element content in patients with lung cancer during radiotherapy |
| title_full_unstemmed | The dynamics of element content in patients with lung cancer during radiotherapy |
| title_short | The dynamics of element content in patients with lung cancer during radiotherapy |
| title_sort | dynamics of element content in patients with lung cancer during radiotherapy |
| topic | Ядернo-физические методы и обработка данных |
| topic_facet | Ядернo-физические методы и обработка данных |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/96428 |
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