Specific Differences in AMELX and AMELY Genes from Sumatran Tigers, Panthera tigris sumatrae (Felidae), for Molecular Sex Identification
Sex determination by DNA-based molecular techniques in Sumatran tigers needs to be investigated and developed for forensic and population genetic purposes. The amelogenin gene is a marker commonly used for sex determination. In some species, the difference between the AMELX and AMELY sequences has b...
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Publishing House "Akademperiodyka" of the National Academy of Sciences of Ukraine
2024
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oai:ojs.akademperiodyka.org.ua:article-4762024-08-20T08:50:37Z Specific Differences in AMELX and AMELY Genes from Sumatran Tigers, Panthera tigris sumatrae (Felidae), for Molecular Sex Identification Asrori , I. Tjong, D. H. Novarino, W. Roesma, D. I. tiger DNA sexing intron sequence Sex determination by DNA-based molecular techniques in Sumatran tigers needs to be investigated and developed for forensic and population genetic purposes. The amelogenin gene is a marker commonly used for sex determination. In some species, the difference between the AMELX and AMELY sequences has been reported to be in the intron region. However, the difference between the AMELX and AMELY sequences in the Sumatran tiger is unknown. Therefore, it is necessary to investigate the sequence differences in introns between AMELX and AMELY Sumatran tigers to determine the specific differences between male and female samples. This study aimed to analyse the sequence of nucleotide bases in the Sumatran tiger amelogenin gene introns based on the nucleotide base sequences in the amelogenin gene introns. The method in this research is descriptive, with a molecular observation of the AMELX and AMELY Sumatran tiger sequences. The amplified samples were sequenced, and it was found that the lengths of the AMELX and AMELY Sumatran tiger sequences were 215 bp and 194 bp, respectively, with a 21 bp deletion in the AMELY sequence. Publishing House "Akademperiodyka" of the National Academy of Sciences of Ukraine 2024-06-10 Article Article application/pdf https://ojs.akademperiodyka.org.ua/index.php/Zoodiversity/article/view/476 10.15407/zoo2024.04.299 Zoodiversity; Vol. 58 No. 4 (2024): Zoodiversity Zoodiversity (Vestnik Zoologii); Том 58 № 4 (2024): Zoodiversity 2707-7268 2707-725X 10.15407/zoo2024.04 en https://ojs.akademperiodyka.org.ua/index.php/Zoodiversity/article/view/476/282 Copyright (c) 2024 ikrima asrori, Dewi Imelda Roesma, Djong Hon Tjong |
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Zoodiversity |
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| datestamp_date |
2024-08-20T08:50:37Z |
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OJS |
| language |
English |
| topic |
tiger DNA sexing intron sequence |
| spellingShingle |
tiger DNA sexing intron sequence Asrori , I. Tjong, D. H. Novarino, W. Roesma, D. I. Specific Differences in AMELX and AMELY Genes from Sumatran Tigers, Panthera tigris sumatrae (Felidae), for Molecular Sex Identification |
| topic_facet |
tiger DNA sexing intron sequence |
| format |
Article |
| author |
Asrori , I. Tjong, D. H. Novarino, W. Roesma, D. I. |
| author_facet |
Asrori , I. Tjong, D. H. Novarino, W. Roesma, D. I. |
| author_sort |
Asrori , I. |
| title |
Specific Differences in AMELX and AMELY Genes from Sumatran Tigers, Panthera tigris sumatrae (Felidae), for Molecular Sex Identification |
| title_short |
Specific Differences in AMELX and AMELY Genes from Sumatran Tigers, Panthera tigris sumatrae (Felidae), for Molecular Sex Identification |
| title_full |
Specific Differences in AMELX and AMELY Genes from Sumatran Tigers, Panthera tigris sumatrae (Felidae), for Molecular Sex Identification |
| title_fullStr |
Specific Differences in AMELX and AMELY Genes from Sumatran Tigers, Panthera tigris sumatrae (Felidae), for Molecular Sex Identification |
| title_full_unstemmed |
Specific Differences in AMELX and AMELY Genes from Sumatran Tigers, Panthera tigris sumatrae (Felidae), for Molecular Sex Identification |
| title_sort |
specific differences in amelx and amely genes from sumatran tigers, panthera tigris sumatrae (felidae), for molecular sex identification |
| description |
Sex determination by DNA-based molecular techniques in Sumatran tigers needs to be investigated and developed for forensic and population genetic purposes. The amelogenin gene is a marker commonly used for sex determination. In some species, the difference between the AMELX and AMELY sequences has been reported to be in the intron region. However, the difference between the AMELX and AMELY sequences in the Sumatran tiger is unknown. Therefore, it is necessary to investigate the sequence differences in introns between AMELX and AMELY Sumatran tigers to determine the specific differences between male and female samples. This study aimed to analyse the sequence of nucleotide bases in the Sumatran tiger amelogenin gene introns based on the nucleotide base sequences in the amelogenin gene introns. The method in this research is descriptive, with a molecular observation of the AMELX and AMELY Sumatran tiger sequences. The amplified samples were sequenced, and it was found that the lengths of the AMELX and AMELY Sumatran tiger sequences were 215 bp and 194 bp, respectively, with a 21 bp deletion in the AMELY sequence. |
| publisher |
Publishing House "Akademperiodyka" of the National Academy of Sciences of Ukraine |
| publishDate |
2024 |
| url |
https://ojs.akademperiodyka.org.ua/index.php/Zoodiversity/article/view/476 |
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