Вплив нових потенційних фунгістатичних агентів на деякі функції клітин крові людини

• Дата: 2014
• Автори: Kalashnіkova, L. E., Kopernyk, I. M., Semenyuta, I. V., Golod, L. P., Hodyna, D. M., Metelytsya, L. O.
• Формат: Стаття
• Мова: Ukrainian
• Опубліковано: National University of Pharmacy 2014
• Теми: in vitro screening; oxazoles; nitrogen-containing bisphosphonates; functional biosensors; UDC 615.076; in vitro скрининг; оксазолы; азотсодержащие бисфосфонаты; функциональные биосенсоры; УДК 615.076; in vitro скринінг; оксазоли; азотовмісні бісфосфонати; функціональні біосенсори
• Онлайн доступ: https://ophcj.nuph.edu.ua/article/view/ophcj.14.786
• Назва журналу: Journal of Organic and Pharmaceutical Chemistry

Erythrocytes and neutrophilic leukocytes of human blood have been used as biosensors for in vitro screening of new biologically active functionally substituted oxazoles and nitrogen-containing bisphosphonates with the known fungistatic activity. It has been found that none of the compounds under research do not reveal the hemolytic action in the reaction of osmotic hemolysis of human red blood cells. For all compounds tested the erythrocyte sedimentation rate is changed. The functionally substituted oxazoles decreased the reaction rate approximately by 70%, and nitrogen-containing bisphosphonates increased it approximately by 30%. The reactivity found can indicate high affinity of the compounds studied to erythrocyte membranes adrenoreceptors. It can be used for characteristics and prediction of a number of other potential effects of the antimycotical compounds tested.According to the NBT-test the functionally substituted oxazoles are activators of the antimicrobial potential of neutrophilic leukocytes and can be considered as potential stimulators of nonspecific human immunity reactivity. The experimental data obtained significantly expand the range of biological effects of the fungistatic compounds synthesized and confirm perspectiveness of their further study as antimycotic agents. In vitro screening with the use of human blood cells as biosensors is an important part of potential drugs research and can be recommended for testing new biologically active compounds with the known biological activity.