Розробка методик ідентифікації та кількісного визначення Епіфіну – нового потенційного антиконвульсанта

• Дата: 2015
• Автор: Perekhoda, L. O.
• Формат: Стаття
• Мова: English
• Опубліковано: National University of Pharmacy 2015
• Теми: pharmaceutical analysis; identification; assay; anticonvulsant; UDC 615.213; 658.562.47; 547.791.1; фармацевтический анализ; идентификация; анализ; антиконвульсант; УДК 615.213; фармацевтичний аналіз; ідентифікація; аналіз
• Онлайн доступ: https://ophcj.nuph.edu.ua/article/view/ophcj.15.840
• Назва журналу: Journal of Organic and Pharmaceutical Chemistry

According to the results of the pharmacological testing the substance of 4-methoxy-3-chloroanilide 1-(3’-fluorophenyl)-5-methyl-1,2,3-triazole (1H)-4-carboxylic acid has shown a pronounced anticonvulsant activity that exceeds the level of the reference drug – Depakine. It is recommended for further profound studies under conditional name Epifine. Development of methods for the quality control of a substance occupies an important place among the stages of drug introduction.For preparation of the substance to introduction we have developed the methods for identification and quantitative determination of the promising substance Epifine. Standardization has been performed in accordance with the existing requirements for development of modern methods for analysis of pharmaceutical substances possessing the pharmacopoeial quality. For identification a set of physical, physicochemical (NMR 1H, IR and UV spectroscopy) and chemical methods has been proposed. The physical and chemical properties of Epifine have been investigated, and the intervals of melting point and solubility in different solvents have been determined. As expected, in the IR spectrum the characteristic absorption bands confirming the presence of aromatic rings, in particular benzene and triazole, carbonyl groups (amide-1, amide-2), substituted amino group, methyl and methoxy groups, were observed. We have also proposed the chemical methods of identification of this compound conditioned by the presence of the anilide residue, the triazole ring, and covalently bound halogens. The non-aqueous acid-base titration has been developed for the quantitative analysis of Epifine.