Изучение жизнеспособности микроорганизмов и сохранности структурных участков их ДНК, амплифицируемых в ПЦР, после низкотемпературного хранения в различных условиях

The obtained experimental data testify to the fact that under low temperature preservation the cyclic changes in storage temperature from –196 to –100°C can result in additional death of microbial cells. In microbial cells, stored under liquid nitrogen constant temperature and under conditions...

Повний опис

Збережено в:
Бібліографічні деталі
Дата:2003
Автори: Vysekantsev, I. P., Omelchenko, E. A., Gurina, T. M.
Формат: Стаття
Мова:English
Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2003
Теми:
Онлайн доступ:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1013
Теги: Додати тег
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Назва журналу:Problems of Cryobiology and Cryomedicine

Репозитарії

Problems of Cryobiology and Cryomedicine
Опис
Резюме:The obtained experimental data testify to the fact that under low temperature preservation the cyclic changes in storage temperature from –196 to –100°C can result in additional death of microbial cells. In microbial cells, stored under liquid nitrogen constant temperature and under conditions of storage temperature variation within the mentioned range, there are preserved the DNA structural sites, providing the amplification in polymerase chain reaction (PCR) of specific fragments.