Изучение жизнеспособности микроорганизмов и сохранности структурных участков их ДНК, амплифицируемых в ПЦР, после низкотемпературного хранения в различных условиях

• Date: 2003
• Main Authors: Vysekantsev, I. P., Omelchenko, E. A., Gurina, T. M.
• Format: Article
• Language: English
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2003
• Subjects: мікроорганізми; кріоконсервування; ДНК; ПЦÐ
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1013
• Journal Title: Problems of Cryobiology and Cryomedicine

The obtained experimental data testify to the fact that under low temperature preservation the cyclic changes in storage temperature from –196 to –100°C can result in additional death of microbial cells. In microbial cells, stored under liquid nitrogen constant temperature and under conditions...