Влияние ДМСО на фазовые переходы и стеклование в суспензии эритроцитов кордовой крови ниже 0°C

Using the DSC method there were investigated the cord blood erythromass, mixed in 1:1 ratio with DMSO aqueous solutions of different concentrations, as well as the samples of dense sediment and supernatant fluid, obtained after these mixtures’ centrifugation. The temperatures of vitrification, dev...

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Збережено в:
Бібліографічні деталі
Дата:2006
Автори: Zinchenko, A. V., Bobrova, E. N., Schetinskiy, M. I.
Формат: Стаття
Мова:English
Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2006
Теми:
Онлайн доступ:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1026
Теги: Додати тег
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Назва журналу:Problems of Cryobiology and Cryomedicine

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Problems of Cryobiology and Cryomedicine
Опис
Резюме:Using the DSC method there were investigated the cord blood erythromass, mixed in 1:1 ratio with DMSO aqueous solutions of different concentrations, as well as the samples of dense sediment and supernatant fluid, obtained after these mixtures’ centrifugation. The temperatures of vitrification, devitrification, crystallisation and melting of eutectic compositions and the temperature of complete melting of systems were determined. There was built a part of diagram for physical states of water-DMSO binary system within the concentration range from 0 up to 60 % (w/w) of DMSO. Using the NMR method it was shown, that the DMSO concentration in supernatant fluid was higher, than in a dense suspension of erythrocytes. This explains the differences in eutectics crystallisation in the samples of erythrocyte dense suspension and in supernatant fluid.